The growing need to cater the energy demand coupled with the urge to mitigate GHG footprint in the energy sector has necessitated inventorying of renewable and sustainable energy alternatives. Green seaweeds have the potential to serve as a renewable and sustainable feedstock for bioethanol production. Dilute acid, H₂SO₄ is an appropriate pretreatment method for extraction of sugars from algal biomass. Algal biomass pretreated using dilute acid yields higher sugar during enzyme hydrolysis by exposing cellulose fibres for enzyme degradation. Neutralization using Na₂CO₃ exhibited lower sugar removal for U.lactuca (39.8%) and E.intestinalis (14.7%). Green seaweeds U.lactuca and E. intestinalis are viable feedstock for bioethanol production due to higher carbohydrate content of 62.15% and 40.1% respectively. Saccharification and Fermentation (SSF) process yielded higher conversion efficiency compared to SHF (Separate hydrolysis and fermentation) process and SSF achieved higher efficiency for U.lactuca indicating fermentation of more than one sugar present in the medium. Toddy yeast strain exhibited higher efficacy in fermentation of E.intestinalis hydrolysate in both SHF and SSF process achieving 25.8% and 77.4% efficiency respectively. Macroalgal biomass has potential to produce various value added products of commercial significance in addition to the production of biofuels. The study highlights the scope for bioethanol from macroalgae and also the availability of potential sites for cultivation. The comprehensive knowledge of macro algal resources distribution, seasonality and biochemical composition through field survey and choice of appropriate enzyme and yeast would aid in realizing the path of the sustainable, economical and commercially viable biofuel.