Conclusions

Indoor and outdoor culture conditions could support good growth of specific strains of fresh water algae evident from the community structure. The lipid characterization of the consortium of algae provided insights into applying mixed population for enhanced lipid productivity and hence biofuel. This study established the proof-of-concept for production of biodiesel from a consortium of algae from both indoor as well as outdoor culture conditions. The changes in neutral lipid emphasize the importance of knowing how nutrient levels play an important role in each of the microalgae for an enhanced accumulation of neutral lipids. Modifications using genetic engineering can be used to convert the autotrophic microalgae to heterotrophic microalgae which can accumulate maximum oils. For further application of this technique, role of each keystone microalgae species in the contribution towards lipid production in a consortium with its ecological preference has to be studied. This can be achieved by not limiting the consortium to just one alga, but for other classes as well. Biodiesel produced from the mass cultivation of microalgae potentially offers a high attractive and ecologically friendly biodiesel but after almost half a century of research the full promise of microalgae as a feed stock for biofuel production has remained largely unfulfilled.