Conclusions

The present study demonstrates the potential of E. intestinalis and U. lactuca as marine source for production of sugar for biofuel production. Feedstock E. intestinalis and U. lactuca were subjected to dilute acid pretreatment yielded 239.94±1.3 mg g^-1 and 214.67±0.9 mg g^-1of reducing sugar. An enzyme extracted from marine bacteria Vibrio parahaemolyticus hydrolysed the algal biomass efficiently, releasing one-fold higher reducing sugar than dilute acid pretreatment. Enzymatic hydrolysis of pretreated macroalgal biomass produced 1-fold higher reducing sugar than the dilute acid pretreatment. Dilute acid pretreatment prior to Enzymatic hydrolysis improves algal biomass saccharification and releases higher reducing sugar, increasing bioethanol yield. However, tailor-made approaches need to be employed depending on algal species, as some sugars are easily hydrolysed by acid and few by a direct enzyme.