1Energy and Wetlands Research Group, Centre for Ecological Sciences [CES],
Indian Institute of Science, Bangalore – 560012, India.
2 Centre for Sustainable Technologies [CST], Indian Institute of Science.
3Centre for Infrastructure, Sustainable Transport and Urban Planning [CiSTUP],
Indian Institute of Science, Bangalore 560 012.
*Corresponding author:
trv@iisc.ac.in
Conclusion
The Cladosporium tenuissimum CS4 fungal strain extracted lipase has proven
characteristics to be a low-cost
biocatalyst over con- ventional acid catalysts for microalgal biodiesel production with
assured
environmentally
friendly bioproducts. Cladosporium sp. extracted lipase being used as a biocatalyst
for
optimizing micro-
algal biodiesel production is reported for the first time in this study. Biochemical and
enzyme
characterization of
such thermotolerant enzyme could aid as an industrially important enzyme serving diverse
interdisciplinary
applications. The extracted and purified enzyme is effective in catalyzing the conversion of
microalgal oil into
biodiesel with higher efficiency (87.2 ± 0.47%) compared to the conventional acid catalyst
(83.02
± 0.35%). Future
studies on pro- tein characterization, sequencing and enzyme immobilization us- ing
different
solid substrate
sources or chemical methods are expected to improve the enzyme viability and reuse.
Data and accessibility
Data used in the analyses are compiled from the field. Data is anlysed and organized in the
form
of table, which are
presented in the manuscript. Also, synthesized data are archived at http://wgbis.ces.iisc.ernet.in/energy/paper/researchpaper.
html#bio.
http://wgbis.ces.iisc.ernet.in/biodiversity/
Funding
The authors acknowledge the financial support from ISRO e IISc space technology cell
[ISTC/BES/TVR/389] and Gajanana
and Mad- havi Hegde [CES/TVR/GJH001] endowment for biofuel research.
Research ethics
The publication is based on the original research and has not been submitted elsewhere for
publication or web
hosting.
Animal ethics
The research does not involve either humans, animals or tissues.
Permission to carry out fieldwork
Ecological research carried by us in the Western Ghats is commissioned by the Ministry of
Environment, Forests and
Climate Change (ENVIS Division), Government of India and hence no further permission is
required
as the field work
was carried out in the non- restricted areas/protected areas.
Declaration of competing interest
On behalf of all authors, the corresponding author states that there is no conflict of
interest.
CRediT authorship contribution statement
G. Saranya: Data curation, Writing - original draft. T.V. Ram- achandra: Formal analysis,
Writing
- review &
editing.
Acknowledgements
The authors acknowledge the financial support from ISRO e IISc space technology cell
[ISTC/BES/TVR/389] and Gajanana
and Mad- havi Hegde [CES/TVR/GJH001] endowment for biofuel research. We are grateful to the
researchers at Prof. D.
N Rao’s lab, Department of Biochemistry, Indian Institute of Science for providing the
infra-
structure support,
guidance and useful inputs for enzyme charac- terization and purification. We thank central
facilities of IISc e
Society for Innovation and Development for Mass spectroscopy (GC-MS) analysis, Advanced
Facility
for Microscopy
and
Microanalysis for SEM imaging and Micro Nano characterization Facility
(MNCF) for FT-IR
analysis.