ID: 59820
Title: PSEUDALLESCHERIA BOYDII IN WILD RODENTS IN KERALA, INDIA.
Author: Manuel Thomas, Rogimon P.Thomas and M.Thangavel.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 107-108 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Psedallescheria boydii, Wild rodents, Kerala.
Abstract: During mycological examination of wild rats in Kerala, P. boydii was isolated from lungs of two species of rats viz water rats (Rattus norvegicus) and larger bandicoot rat (Bandicota indica). The lung samples were inoculated into Sabouraud Dextrose Agar (SDA) with antibiotics and incubated at room temperature. The developed colonies were further subcultured on to SDA, Potato Dextrose Agar (PDA) and Cornmeal Agar (CMA). Typical white colonies which alter becomes dark gray to smoky brown with ruptured cleisthothecium-the hallmark of P. boydii was observed. Among the tested rodent species, 15 % of Rattus norvegicus specimens and 10 % of Bandicota indica were found positive for P. boydii.
Location: TE 12 New Biology Building
Literature cited 1: Annam, V., Athaniker, V.S. and Yeliker, B.R. 2008. Isolated frontal sinusitis due to Pseudallescheria boydii. Indian J. Pathol. Microbiol. 51: 435-436.
Cortez, K.J., Roilides, E., Quiroz-Telles, F., Meletiadis, J., Antachopoulos, C., Knudsen, T., Buchanan, W., Milanovich, J., Sutton, D.A., Fothergill, A., Rinaldi, M.G. and Shea, Y.R. 2008. Infections caused by Scedosporium spp. Clin. Microbiol. Rev. 21 (1): 157-197.
Literature cited 2: Davis, P.R., Meyer, G.A., Hanson, R.R. and Stringfellow, J.S.2000. Pseudallescheria boydii infection of the nasal cavity of a horse. Journal of the American Veterinary Medical Association. 217 (5): 617-792.
Guaro, J., Kantarcioglu, A.S., Horre, R., Rodriguez-Tudela, J.L., Estrella, M.C., Berenguer, J. and de Hoog, G.S. 2006. Scedosporium apiospermum: Changing clinical spectrum of a therapy-refractory opportunist. Med. Mycol. 44: 295-327.
ID: 59819
Title: ASSESSMENT OF POLLUTION IN MIR ALAM LAKE, HYDERABAD, A.P., INDIA.
Author: Amin Hossaini Motlagh, K. Navatha and P.Manikya Reddy.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 103-106 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pollution, Water quality, Mir Alam Lake, Phytoplankton.
Abstract: An assessment of pollution of the Mir Alam Lake has been made with the help of chemical and phycological parameters. Four stations were selected within the lake. Biocarbonates, Chlorides, Phosphates, Nitrites and Total solids were recorded in very high concentrations. Dissolved oxygen was present in low levels in the lake. The biological data reveal that the individuals of some organisms have increased. The data compared with ISI and WHO standards and water quality criteria. The importance of algae as biomonitors in pollution studies has also been emphasized.
Location: TE 12 New Biology Building
Literature cited 1: APHA, AWWA and WPCF, (American Public Health Association, American Water Works Association and Water Pollution Control Federation). 1995. Standard Methods for the Examination of Water and Waste Water 19th ed. Published by APHA, AWWA and WPCF, Washington, USA.
Ashesh Tiwari and Chauhan, V.S. 2006. A check list of Chlorococcales from Bandh Baretha water reservoir at Bharatpur, Rajasthan. J. Indian Bot. Soc. 86 (1-2): 43-47.
Literature cited 2: Butcher, R.W. 1949. Problems of distribution of sessile algae in running water. Verm. Inst. Ver. Limnol. 10: 98-103.
Cairns, J. and Lanza, G.R. 1972. Pollution controlled changes in algal and Protozoan communities in water pollution. Microbiology (Ed. R Mitchell, John Wiley and Sons.) Inc. pp. 245-273.
ID: 59818
Title: BIOEFFICIENCY AND CYTOTOXIC EFFECT OF AQUEOUS LEAF EXTRACT OF ANNONA MURICATA LINN ON ROOT TIPS OF ALLIUM CEPA LINN.
Author: M.Mini and P.M. Beebi Razeena.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 97-101 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Annona muricata Linn, Acetogenins, Bioefficacy, Cytotoxicity, Conservation.
Abstract: Annona muricata Linn belongs to Annonaceae family called ' Soursop tree ' (vernacular name- ' Mullenchakka ' or ' lakshmana Pazham ' ) and can grow in low elevated areas of Kerala. The present study focuses on bioefficiency and cytotoxic activity of the leaf of this plant on root meristem of Allium cepa Linn. In vitro studies of aqueous leaf extract showed inhibition of the growth of Aspergillus flavus Link; Fr, Aspergillus fumigatus and aspergillus niger Van Tieghem. The cytotoxic study of different concentration of Annona muricata leaf extract (90 %, 80 %) on root meristem of Allium cepa Linn showed chromosome aberrations (chromosome bridges, rings, lagging and fragmentations, diagonal Anaphase and metaphase, vacuolated and sickle shaped nucleus, ball metaphase and Micronuclei). In 60 % and control have no chromosomal aberrations. This study again proved this plant can prevent the pathological malignancy of the cells. Nowadays this plant is endangered and threatened. At these circumstances this plant must protect and conserve for the welfare of human beings.
Location: TE 12 New Biology Building
Literature cited 1: Arthur, F.K.N., Woode, E., Terlahi, E.O. and Larbie, C. 2011.Evaluation of acute and subchronic toxicities of Annona muricata Linn aqueous extract in animals. Euro. J. Exp. Bio. 1 (4): 115-124.
Chang, F.R. and Wu, Y.C.2001. Novel cytotoxic Annonaceous Acetogenins from Annona muricata. J. Nat Prod 64 (7): 925-931.
Literature cited 2: Gajalakshmi, S., Vijayalakshmi, S., and Devi Rajeswari, V. 2012. Phytochemical and pharmacological properties of Annona muricata: a review. Int. J. of Pharmacy and Pharmaceutical Science. 4 (2): 3-6.
Kim, G.S., Zens, L., Alali, F., Rogrs, L.L., Wu, F.E., Sastrodihardjo, S. and Mc Laughlin, J.L. 1998. Muricoreasin and murihexocin C Mono tetrahydrofuran acetogenins from the leaves of Annona muricata. J. of Phytochemistry. 49 (2): 565-571.
ID: 59817
Title: PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF MEDICINAL PLANTS.
Author: Sonali Dilip Randive.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 91-96 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Phytochemical, Medicinal Plants, Antimicrobial activity.
Abstract: The phytochemical analysis and antimicrobial activity of six medicinal plants like caesalpinia bonducella Murraya koengi, Tinospora cordifolia, Morinda citrifolia, Ficus fistulosa, Roasted kirkasaali have been tested for their antimicrobial activity against pathogenic organisms Bacillus subtilis staphylococcus aureus, E. coli, Pseudomonas aueroginosa, Klebsiella pneumoniaalong.Caeselpinia bonducella show excellent antimicrobial activity against S. aureus and E.coli Bacillus subtilis, Murraya koenigii show activity against Klebsiella pneumonia Tinospora cordifolia show good activity against Klebsiella pneumonia and E.coli.
Location: TE 12 New Biology Building
Literature cited 1: Acar, J.F. and Goldstein F.W., 1996, Disk Susceptibility Test In Antibiotics In Laboratory Medicine. 4th edition, edited by victor L, Editor (Williams and Wilkins publishers) 1-51.
Agarry, O.O., M.T. and Bello Michael, C.O. 2005. Comparative antimicrobial activities of Aloe vera gel and leaf. African Journal of Biotechnology. 4 (12): 1413-1414.
Literature cited 2: Ahmad, M.M., Salim-ur-Rehman, Z. Iqbal, F.M. Anjum and Sultan, J.I. 2006. Genetic variability to essential oil composition in four citrus fruit species. PakJ. Bot. 38 (2): 319-324.
Almas, K., and Ansallafi, T.R. 1995. The natural toothbrush. World Health Forum. 16: 206-210.
ID: 59816
Title: ECO-FRIENDLY DOSE OF ANTIBIOTICS FOR SUPPRESSION OF AGROBACTERIUM TUMEFACEINS.
Author: J.Rastogi, N.N Tiwari, S.Gupta, P.Bubber and R.K.Singh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 85-87 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Agrobacterium tumefaciens, Sugarcane, Antibiotics, Eco-friendly
Abstract: One of the major obstacles in sugarcane transformed tissue is the persistence of Agrobacteriaum tumefaciens after co-cultivation. Many antibiotics are used to elimi8nate the bacteria. Antibiotics interrupt the synthesis of bacterial cell wall and also suppressing the growth of co-cultivating tissues at high concentration. An assay was performed to fix the minimal eco-friendly lethal dose of the eliminating agent that was precisely efficient against A. tumefaciens. The disk diffusion assay and co-cultivation of leaf disc explants were performed by using the A. tumefaciens strain LBA 4404 P-35 GUS Intron having the plasmid PCAMBIA 1301, which carries the selectable marker gene (npt II) for kanamycin. It was also correlated whether these antibiotics at high concentration inhibit growth of sugarcane callus and shoot formation. In this study five antibiotics i.e. Cefotaxime, Erythromycin, Chloramphenicol, Amoxicillin and Ciprofloxacin were tested with seven different concentrations @ 50, 100, 150, 200, 300, 400 & 500 mg/1 along with control. The antibiotics which showed maximum zones of inhibition were assayed to determine their effects on shoot growth and elimination of A. tumefaciens. Maximum inhibition was observed with Chloramphenicol @ 50 mg/1 against A. tumefaciens LBA 4404 P-35 GUS Intron without any adverse effect on regenerated shoots through young leaf disc. The Cefotaxime @ 200 mg/ 1 was found effective to eliminate A. Tumefaciens LBA 4404 P-35 GUS Intron eco-friendly, an efficient and cost-effective transformation system in sugarcane.
Location: TE 12 New Biology Building
Literature cited 1: Arvinth, S., Arun, S., Selvakesavan, R.K. Sikanth, J., Mukunthan, N., Ananda, P.K., Premchandran, M.N. and Subramonian, N. 2010. Genetic transformation and pyramiding of aprotinin-expressing sugarcane with cry1 Ab for shoot borer (Chilo infuscatellus ) resistance. Pant Cell Rep. 29: 383-395.
April. 2012. Amoxicillin spectrum of bacterial susceptibility and Resistance. www. Toku-e.com.
Literature cited 2: Christy, L.A., Arvinth, S., Sarvanakumar, M., Kanchana, M., Mukunthan, N., Srikanth, J., Thomas, G. and Subramonan, N. 2009. Engineering sugarcane cultivars with bovine pancreatic trysin inhibitor (aprotinin) gene for protection against top borer (Scripophaga excerptalis Walker) Plant Cell Rep. 28: 175-184.
Dessoky, S.E., Roba, M.I., Abdel, H.A., Abdel, H. and Naglaa, A. 2011. Establishment of regeneration and transformation system of sugarcane cultivar GT54-9 (C9). GM Crops. 2 (2): 126-134.
ID: 59815
Title: PHYTOCHEMICAL AND ANTIINFLAMMATORY STUDIES OF STEM BARK EXTRACT OF DELONIX ELATA
Author: T.Raga Sudha and B. Ganga Rao.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 81-83 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Delonix elata, Anti-inflammatory activity, Stem bark, Phyto-chemical activities.
Abstract: Delonix elata (Fabaceae) is an ornamental and medicinal tree found in Africa and Asia. The methanolic extract of the stem bark in Delonix elata was screened for anti-inflammatory activity in carrageenan-induced rat paw oedema at the dose levels of 100, 200 and 400 mg/kg was compared with the standard drug ibuprofen. The extract produced significant and dose dependent inhibition of carrageenan-induced paw oedema. The results were found to be significant (P<0.001) when compared to control. Preliminary phytochemical analysis of Delonix elata indicates the presence of triterpinoids, flavonoids and tannins. Further studies may reveal the exact mechanism of action and phytochemical constituents responsible for anti-inflammatory activity.
Location: TE 12 New Biology Building
Literature cited 1: Alj Habaubi and Zeitlin, I.J.1983. Europ. J. Pharmacol. 88: 1969.
Anonymous Pharmacopoeia of India. 1980. 2nd Edn, Ministry of Health, New Delhi, 67-69.
Literature cited 2: Azza Agh, M. and Gad, Z. 1995. Lipid peroxidation and lysosomal integrity different inflammatory models in rats. The effects of indomethacin and naftazone. Pharmacol. Res. 329: 279-285.
Cragg, G.M. and Newman, D.G. 2001. Medicines from the millennia. Ann. NY Acad. Sci. 953: 3-25.
ID: 59814
Title: PRODUCTION AND CHARACTERIZATION OF XYLANASE FROM LOCAL FUNGAL ISOLATES BY SUBMERGED FERMENTATION.
Author: Varsha D.Savanth and Seema J. Patel.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 75-80 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Aspergillus sp, Hhydrolysis, Zymogram, Dialysis.
Abstract: Xylanases showing better stability of alkaline pH and higher temperatures have potential applications in several industrial processes. The objective of this study is to determine the kinetic properties and thermal and alkaline stability of partially purified xylanase from Aspergillus niger isolates using birch wood xylan as carbon source. The fungal strains were isolated from soil of different places around Davangere, identified and preserved. Xylanase was extracted by submerged fermentation using these isolates on standard substrate. Further it was purified by ammonium sulfate (80 %) fractionation and dialysis. The molecular mass of the xylanase was ~ 22 kDa and the zymogram analysis indicated that this enzyme was an active xylanase. Highest xylanase activity of 1.222 units.mL-1 was obtained with the isolate 5. The optimum temperature was found to be 40 ? C and pH 4.5. In the presence of metal ions such as Mg2+ and Cu2+, the activity of the enzyme increased, whereas strong inhibition of the enzyme activity was observed in the presence of Hg+2 and chelator EDTA. The hydrolysis study of birch wood xylan by TLC showed the formation of xylose as an end product.
Location: TE 12 New Biology Building
Literature cited 1: Abu, E.A., Onyenekwe, P.C., Ameh, D.A., Agbaji, A.S. and Ado SA. 2000. Cellulase (E.C.3.2.1.3) production from sorghum bran by Aspergillus niger SL 1: An assessment of pretreartment methods. Proceedings of the International Conference on Biotechnology: Commercialization and Food security, Abuja, Nigeria. 153-159.
Anand, L.Krishnamurthy, S. and P.J. Vithayathil, 1990. Purification and properties of xylanase from the thermophilic fungus, Humicola lanuginose (griffon and manublanc) bunce, Arch, Arch.Biochem. Biophys. 276: 546-553.
Literature cited 2: Bhat, P.1985. Microbial xylanolytic system.Trends. Biotechnol. 3 (2): 286-290.
Bhatt Nikhil, Adhyaru Dharmesh and Thakor Eriksson, K-EL. 1985. Swedish developments in biotechnology related to the pulp and paper industry.Tech. Assoc. Pulp. Pap. Ind. 68: 46-55.
ID: 59813
Title: SCREENING OF ANTIVIRTAL ACTIVITY OF THE CHOSEN SEAWEED EXTRACT.
Author: D.Jenilarani, M.Millicent Mabel, N.Parthasarthy and A.Uma.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 70-74 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Seaweeds, Shrimp, Enteromorpha sp., Ulva sp. and Sargassum sp.
Abstract: Seaweeds are possessed anti-viral properties that was designed to evaluate the antiviral properties of selected seaweeds namely, Enteromorpha sp., Ulva sp. and Sargassum sp. in controlling the White Spot Sundrome disease in shrimp. In the present investigation found the viral infection cannot be completely inhibited, the methanol extract from Enteromorpha sp. reduced the effect of the virus up to its 3rd dilution (200 mg with 400 mL medium/well) against WSSV. The peeling off cells was observed to be lesser in the infected cells treated with the extract in this concentration when compared to the wells infected with WSSV (virus control). The significant anti-viral activity was observed up to its 3rd dilution in Ulva sp. extracted with chloroform and at 4th and 5th dilutions in Ulva sp. extracted with hexane.
Location: TE 12 New Biology Building
Literature cited 1: Amin Ismail and Tan Siew Hong, 2002. Anti-oxidant activity of selected commercial seaweeds. J. Nutr. 8 (2): 167-177.
Ballesteros, E and D. Martin, 1992. Biological activity of extracts from Mediterranean Sea. Bot. Mar. 35: 481-485.
Literature cited 2: Blunden, G., Barwell, C.J and Jewers, K. 1981. A survey of some British marine algae for anti-influenza virus activity. Bot. Mar. 24: 478-488.
Caccamese, S.R., Azzolina, Furnari, G. and Cormaci, M. 1981. Antimicrobial and antiviral activities of some marine algae from eastern sicily.Bot.Mar. 24: 365-367.
ID: 59812
Title: OPTIMIZATION OF BIODIESEL PRODUCTION FROM WASTE COOKING OIL USING PURE LIPASE AND RHIZOPUS ORYZAE THROUGH RESPONSE SURFACE METHODOLOGY.
Author: B. Bharathiraja, A. Saravana Raj, J.Jayamuthunagai, M. Jayakumar, M.Arul Kirubakaran, P. Vivek, R. Praveen Kumar and S.Palani.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 63-70 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Response surface methodology, Transesterification, Fatty acid alkyl esters, Acyl acceptor.
Abstract: Biodiesel is the Fatty Acid Alkyl Ester of vegetable oils produced through transesterification reaction. Immobilized pure lipase and Rhizopus oryzae was used as the catalyst for the conversion of Waste Cooking oil (WCO) into biodiesel using methanol as an acyl acceptor. Response surface methodology (RSM) was adopted to optimize the parameters such as temperature, time, oil to solvent ratio, enzyme concentration for lipase and pH, olive oil concentration, rpm for Rhizopus oryzae. A high conversion of 94.01 % was obtained through lipase catalyzed reaction at oil to solvent ratio of 1:3, enzyme concentration of 10 % at 30 ? C after 24 hrs. Similarly, a high conversion of 83.76 % was obtained at an optimum pH of 5.5, olive oil concentration 25 g/L and 150 rpm was obtained for Rhizopus oryzae. The lipase resulted in higher yield of biodiesel when compared to the Rhizopus oryzae. Thus the influential parameters were optimized using RSM.
Location: TE 12 New Biology Building
Literature cited 1: Arjun, B., Chhetri, K., Chris Watts and Rafiqul Islam, M., 2008.Waste Cooking Oil as an Alternate Feedstock for Biodiesel Production. Energies. 1: 3-18.
Bharathiraja Balasubramaniam, Ayyappasamy Sudalaiyadum Perumal, Jayamuthunagai Jayaraman, Jayakumar Mani, Praveen kumar Ramanujam., 2012. Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil). Waste Management. 32: 1539-1547.
Literature cited 2: Box, G.E.P. and Wilson, K.N. 1951. On the experimental attainment of optimum conditions. J. Royal Statist. Soc., Series B. 13: 1-45.
Chen, Y., Xiao, B., Chang, I., Fu, Y., Pengmei, L.V. and Wang, X. 2009. Synthesis of biodiesel from waste cooking oil using immobilized lipase fixed bed reactor. Energy conserve. Manage. 50: 668-673.
ID: 59811
Title: ASSESSMENT OF ANTIMICROBIAL POTENCY OF MACTRA VIOLACEAE CRUDE AND PROTEIN ISOLATES.
Author: M.Arputha Bibiana, M.S. Manikandan, P.Selvamani and S.Latha.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 59-62 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: AMPs, Marine Invertebrate, HPLC, Antimicrobial Assay.
Abstract: The marine source was collected from the coast of Cuddalore, Tamil Nadu and it was extracted with 30 % acetic acid solution and Tris Hcl buffer. The crude was centrifuged to precipitate and precipitated with Ammonium Sulphate. The attained precipitate was dialyzed with 3.0 kDa membrane using magnetic stirrer and retentates were collected. The antimicrobial activity assay of crude protein extracts of Marine invertebrate was tested against microorganisms by agar well plate method. The marine invertebrate species Mactra violaceae was found to respond to produce the antimicrobial activity against E.coli, S. aureus, and P. aeruginosa. Further the crude extract was fractionated by RP-HPLC C-18 with the gradient range of 5-95 % CAN in 0.1% TFA solution. Eight fractions were achieved and the faction eight (F8) were responded with good antimicrobial potency against E.coli, S. aureus and P.aeruginosa. On SDS-PAGE analysis dark band was obtained with 14 k Da compared to marker. The crude protein extract of Mactra violaceze was also characterized by FTIR. The crude protein content of Mactra vuilaceae was estimated by biuret method which gives concentration at 5001g/mL of sample.
Location: TE 12 New Biology Building
Literature cited 1: Anbuselvi, S., Chellaram, C., Jonesh, S. Jayanthi, L. and Edward, 2009. Bioactive Potential of Coral Associated Gastropod, Trochus tentorium of Gulf of Mannar, Southeastern India. J. Med. Sci. 9 (5): 240-244.
Arti Nigam, Archana Ayyagari, 2008. Lab Manual in Biochemistry, Immunology and Biotechnology. 2nd edition. New Delhi: Tata Mcgraw-Hill; 49-50.
Literature cited 2: Boobathy, S., Soundarapandian, P., Subasri, V., Vembu, N.and Gunasundari, V. 2009. Bioactivities of Protein Isolated from Marine Sponge, Sigmadocia fibulatus. Current Research Journal of Biological Sciences. 1 (3): 160-162.
Carlos Lopez-Abarrategui et al. 2012.Screening of antimicrobials from carribean sea animals and isolation of bactericidal proteins from the littoral mollusk cenchritis muricatus. Curr Micribiol 64: 501-505.
ID: 59810
Title: EVALUATION OF PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY ON LEAVES AND BARK OF ALBIZZIA LEBBECK (L) BENTH.
Author: Vasanthi Padmanabhan, Manimekalai Ganapathy, Vasthi Kennedy Evanjelene, Nirmala Ayyavuv and Jagajothi Angamuthu.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 53-58 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Phytochemical, Antibacterial, Albizzia lebbeck.
Abstract: During ancient times the plant were used to cure the diseases and infections. Medicinal plants are cheap and easily available and affordable. The medicinal importance of plants lies in some chemical substances that produce a specific physiological action on the human body. Natural products, due to their easy availability, low cost and lack of side effects, make themselves attractive candidates for drug research. One such plant is Albizzia lebbeck (L) Benth medicinal plant belonging to the family Mimosaceae. The present study was designated to evaluate the antibacterial and phytochemical activity from the leaves and bark of Albizzia lebbeck. The result of hydroalcohol extracts of leaf showed better antimicrobial activity followed by the bark extracts. The extracts of Albizzia lebbeck showed better inhibition towards Escherichia coli and Salmonella typhi when compared with Cefoperazone/Tazobactam.
Location: TE 12 New Biology Building
Literature cited 1: Anpin Raja, R.D., Jeeva, S., Prakash, J.W., Johnson, M. and Irudayaraj, V. 2011. Antibacterial activity of selected etnomedicinal plants from south India. Asian Pacific Journal of Tropical Medicine. 4 (5): 375-378.
Baker, J.T., Borris, R.P. and Carte, B. 1995. Natural product drug discovery and development: new perspective on international collaboration. J Nat Prod. 58: 1325-1357.
Literature cited 2: Balakumar, S., Rajan, S., Thirunalasundari, T. and Jeeva, S. 2011. Antifungalnactivity of Aeglemarmelos (L) Correa (Rutaceae) leaf extract on dermatophytes. Asian Pacific Journal of Tropical Biomedicine. 1 (4): 309-312.
Bauer, A.W., Kirby, W.M., Sheris, J.C. and Turck, M. 1966. Antimicrobial susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 45 (4): 493-496.
ID: 59809
Title: ANTOGONASTIC EFFECT OF IRON IN REDUCING HEAVY METAL TOXICITY IN A MICRO ALGAE-A STEP TOWARDS PHYTOREMEDIATION.
Author: Sini K. Skariah and M.R.Manju
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 47-52 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Heavy metal toxicity, Micro-algae, Phytoremediation.
Abstract: The present study aimed to investigate the effect of ZnSO4 on test species, Spirulina platensis-a fresh water micro algae and how the toxic effect can be reduced by the addition of iron (FeCl3). Phytoremediation is a promising alternative which utilizes active biomass or dead biomass to bind and concentrate heavy metal from the aqueous solution. Different types of biomaterials have different levels of metal uptake. The most promising biomaterial studied is algal biomass. The effect of metal on the biomass, growth rate and mechanism of metal tolerance, there by suggesting the importance of this algae in bioremediation and environmental cleanup operation.
Location: TE 12 New Biology Building
Literature cited 1: Agi, M. Thomson and Muraleedhara Kurup, G. 2010. Heavy metal tolerance & metal induced oxidative stress in Spirulina platensis. AJMBES. 12 (2): 461-68.
Ayehunie, S., Belay, A., Baba, T.W. and Ruprecht, R.M., 1998.Inhibition of HIV1 replication by an aqueous extract of Spirulina platensis [Arthospira platensis], J. Acquir. Immune. Defic. Syndr. Hum. Retrovitrol. 18 (1): 7-12.
Literature cited 2: Baker, A.J.M., 1978. Newphytol. 80: 635.
Chamorro, G., Salazar, M., Favila, L. and Bourges, H. 1996. Pharmacology and Toxicology of Spirulina alga. Rev. Inves. Clin. 48: 389-399.
ID: 59808
Title: A COMPARATIVE STUDYON THE PROTEASE PRODUCING BACTERIA ISOLATED FROM DAIRY EFFLUENTS OF CHENNAI REGION, IDENTITIFICATION, CHARACTERIZATION AND APPLICATION OF ENZYME IN DETERGENT FORMULATION.
Author: Masi Chandran, M.Fazil Ahmed and N.Parathasarathi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 41-46 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pretease, Bacillus subtilis, Pseudomonas species, Pharmaceutical.
Abstract: Protease is an economically important enzyme that is being used in many food industries, pharmaceuticals and manufacturing industries. Since protease has heavy demand commercially, its production reaps riches. Studies were made in different strains producing protease enzyme. We have made an attempt to extract protease from bacterial strains isolated from dairy effluents of Chennai region. Totally 6 dominant strains were isolated from dairy effluents of Chennai region. Totally 6 dominant strains were isolated from dairy effluents collected from AAvin milk factory of Pattaravakam, Madhavaram and Sholinganallur of Chennai. Since, milk and milk products contain major amount of casein, bacteria isolated from the dairy effluents are expected to produce more protease enzyme. Bacillus subtilis (MTCC No. 1790) was used as the control. We checked and compared the protease activities of isolated strains with the control. We found that Pseudomonas species were more potent in protease production than the other isolated bacterial strains and the control.
Location: TE 12 New Biology Building
Literature cited 1: Bhaskar, N., Sudeepa, E.S., Rashmi, H.N. and Selvi, A.T. 2007.Partial purification and characterization of protease of Bacillus proteolyticus-CFR3001 isolated from fish processing waste and its antibacterial activities. Bioresour. Technol. 98: 2758-2764.
Deng, A., Zhang, W.U.J., Zhang, G. and Wen, T. 2010. Purification and characterization of a surfactant stable high-alkaline protease from Bacillus sp. B001.Bioresour. Technol. 101: 7100-7116.
Literature cited 2: Entis, P., Fung, D.F.C., Grifiths, M.W., McIntyre, L., Russell, S., Sharpe, A.N. and Tortorello, M.L. 2001.American Public Health Association. USA.
Gerze, A., Omay, D. and Guvenilir, Y. 2005. Partial purification and characterization of protease enzyme from Bacillus subtillis megatherium. Spring; 121-124: 335-345.
ID: 59807
Title: BIODEGRADATION OF PARAQUAT BY THE NOVEL BACTERIAL STRAIN AEROMONAS VERONII NK67 FROM CASSAVA FIELDS IN THAILAND.
Author: Nuttabodee Viriyawattana and S. Surachat.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 35-40 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Paraquat, Biodegradation, Soil, Bioremediation.
Abstract: The herbicide paraquat residues in Cassava field have been shown to cause adverse human health effects and soil degradation, leading to decreased crop productivity. Bioremediation is the effective way to solve this problem. This study focused on selecting soil bacteria that are capable to degrade paraquat. The 283 soil samples were collected from Cassava field in Nakhon Ratcha Sima provinces, because of paraquat is one of the most widely used chemical herbicides in this area. All 112 isolates have been isolated. Among them, there were 6 isolates tolerance to paraquat concentration 1 mM, including 4 and 2 strains of Clostridium spp. and Aeromonas spp., respectively. The isolates were further tested for their efficacy to degrade paraquat. The results showed that only two strains of Aeromonas spp. (strain NK 66 and NK 67) were able to degrade paraquat. It was found that levels of paraquat decreased to 4.9 ppm (24.36%) and 10.68 ppm (53.4%) after exposed to Aeromonas spp. strain NK 66 and NK67, respectively. Molecular Methods to Identify Aeromonas spp. strain NK 67 using PCR-amplified 16s rRNA genes sequencing were employed. Nucleotide sequence of 16S r RNA from Aeromonas spp.strain 67 was compared with nucleotide sequences from GenBank database by using BLAST program. The results showed that 99 % similar to 16s r RNA from A. veronii strain ATCC35624, according to the results of classification by Bergey ' s Manual of Systematic Bacteriology. Then it can be conclude that this bacteria is A. veronii NK67 (JN880412). This is the first report that A. veronii exhibited high efficiency to degrade paraquat which can be further applied for remediation of paraquat in contaminated areas and going for commercial production.
Location: TE 12 New Biology Building
Literature cited 1: Altschul, S.F., Madden, T.L., Schaffer, A., Zhang, T., Miller, V. and Lipman, D.J. 1997. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Research 25: 3389-3402.
Behki, R.M. and Khan, S.U. 1986. Degradation of atrazine by Pseudomonas: N-delkylation and dehalogenation of atrazine and its metabolites. J. Agric. Food. Chem, 34: 746-749.
Literature cited 2: Cao, M., Moore, C.M. and Helmann, J.D. 2005. Bacillus subtilis Paraquat Resistance is Directed by sM, an Extracytoplasmic Function Sigma Factor, and is Conferred by YQjL and BcrC. Journal of Bacteriology. 187: 2948-2956.
Carr, R.J., Bilton, R.F. and and Atkinson, T. 1985. Mechanism of biodegradation of paraquat by Lipomyces starkeyi. Appl. Environ. Microbiol. 49 (5): 1290-1294.
ID: 59806
Title: ISOLATION, PURIFICATION AND SOME PROPERTIES OF AN EXTRACELLULAR DEPOLYMERASE FROM STREPTOMYCES SP. SSM 5670.
Author: O.Yashchuk, S.S. Miyazaki and E.B. Hermida.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 29-33 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: PHBV depolymerase, Streptomyces sp.
Abstract: Streptomyces sp. SSM 5670 with the ability to degrade a natural polymers, polyhydroxyalkanoates (IPHAs), showed a high activity of the extracellular poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) depolymerase when it was grown in a basal medium containing PHBV as the sole carbon source. The extracellular PHBV depolymerase of the organism could be purified to electrophoretic homogeneity by ion exchange column chromatography and gel filtration approximately 10.5-fold. The recovery of its activity was 20.5 %. The molecular mass of the PHBV depolymerase was estimated to be 24 kDa. The maximum activity was observed near pH 7.5 and 30? C, the activity was lost at temperatures above 45? C. This enzyme might be used to promote the degradation of PHBV products buried in soil, stabilized compost or other environments around room temperature.
Location: TE 12 New Biology Building
Literature cited 1: Allen, A.D., Anderson, W.A., Ayorinde, F. and Eribo, B.E. 2011. P (3HB-co-3HV) depolymerase from streptomyces sp. IN1. Int. Biodeterior. Biodegrad. 65: 777-785.
Bradford, M.M1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72: 248-254.
Literature cited 2: Calabia, B.P. and Tokiwa, Y. 2006. A novel PHB depolymerase from a Thermophillic Streptomyces sp. Biotechnol. Lett. 28: 383388.
Hazer, B. and Steinbuchel, A. 2007. Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applications. Appl. Microbiol. Biot. 74: 1-12.