Enter records

ID: 59835
Title: GGDM MODEL ON VALIDATION OF THE INTERIOR BUIDING LAYOUT DESIGN PARAMETERS TOWARDS ENERGY EFFICIENCY AND USER COMFORT.
Author: Mohammad Pir Mohammadi, Abdullah Sani Ahmad, Gurupiah Mursib, Mohsen Roshan and Mahdi Torabi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 205-210 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Energy Efficiency, User Comfort, Ground Group Decision Making Model.
Abstract: Collective decision making model emerges to be applied in the cases where decision makers are not capable to seat in a meeting. Grounded Group Decision Making (GGDM) is unique since collective decision making aggregated by cross evaluation of decision makers in GGDM process. The GGDM in this study is applied to validate the uncertainty involved in validation of the building interior layout design parameter towards energy efficiency and comfort strategies. This problem is applied as illustration of applicability of GGDM in establishing a benchmark for evaluation of students in energy efficient architectural design. First, a literature review and relevant definition to each interior layout design parameter is presented. Second, a GGDM validation study conducted amongst twenty one lecturers and professional practitioners (industrial collaborators of university) in Faculty of Built Environment, Universiti Teknologi Malaysia. In result of the study seven parameters are validated and rated respectively. In implication of specific result of this study, these are useful for researchers in enhancement of Automated Computer Aided Building towards auto evaluation of students drawing in consideration of comfort and energy efficiency.
Location: TE 12 New Biology Building
Literature cited 1: Arnheim, R. 1974. Art and Visual Perception. University of California Press. Cagan, J., Shimada, K. and Yin, S. 2002. A survey on computational approach of three-dimensional layout problems. Computer-Applied Design, 34: 597-611.
Literature cited 2: Deasy, C.M., and Lasswell, T.E 1985. Designing Places for People. Watson-Guptill. Evans, Gary W., Lepore, Stephen J., Schroeder, and Alex. 1996. The role of interior design elements in human responses to crowding. Journal of Personality and Social Psychology. 70 (1): 41-46.

ID: 59834
Title: EFFECTS OF SECONDARY METABOLITES STATUS OF BACILUS SPP. ON FUNGAL PATHOGEN ALTERNARIA ALTERNATA
Author: T.Angayarkanni and Anitha Subash.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 201-204 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Alternartia alternate, Bacillus subtilis, Salicylic acid, Siderophore and Antiobiotics like surfactin and iturin.
Abstract: Alternaria diseases are among the most common diseases of many plants throughout the world. They affect primarily the leaves, stems, flowers and fruits of annual plants, especially vegetables and ornamentals. In this study the in vitro screening of antagonistic Bacillus spp. against Alternaria alternata revealed that the maximum inhibitory effect on the mycelia growth was exhibited by the isolates AUB4 and AUB3.Maximum siderophore production was observed in the isolate AUB7.Estimation of salicylic acid production by the Bacillus spp. isolates showed that isolate AUB4 produced maximum amount. Crude extract of Bacillus antibiotics showed that isolates AUB6, AUB4, AUB2 AND AUB1 have maximum inhibitory effect on the mycelial growth of Alternaria alternate. The TLC analysis of antibiotics extracted from isolates AUB7 and AUB4 showed Rf values of 0.63 and 0.69 for surfactin and 0.48 and 0.53 for iturin A respectively. Thus, from the present study, it can be concluded that among the several secondary metabolites produced by the Bacillus spp isolates, siderophores, salicylic acid and antibiotics are significantly involved in the suppression of the growth of Alternaria alternate. Among the ten isolates screened, AUB4 and AUB7 effectively controlled the growth of the fungal pathogens. This may be due to the high production of siderophore, salicylic acid and antibiotics like surfactin and iturin.
Location: TE 12 New Biology Building
Literature cited 1: Araujo, F.F, Henning, A.A. and Hungaria, M. 2005. Phytohormones and antibiotics produced by Bacillus Subtillis and their effects on seed pathogenic fungi and on soybean root development. World Journal of Microbiology and Biotechnology. 21: 1639-1645. Cho, S.J., Lim, W.J., Hong, S.Y., Park, S.R. and Yun, H.D. 2003. Endophytic colonization of ballon flower by Antifungal strain Bacillus sp. CY22.J. Biosci.Biotechnology.Biochem. 67: 2132-2138.
Literature cited 2: Deshwal, V.K., Pandey, P., Kang, S.C.and Maheswari, 2003. Rhizobacteria as a biological control agent against soil form plant pathogenic fungi. J. Indian Journal of Experimental Biology. 41: 41: 1160-1164. Gardener, M.B.B. 2004. Ecology of Bacilus and Paenibacillus spp. in agricultural systems. J. Phytopathol. 94: 1252-1258.

ID: 59833
Title: OPTIMAL PRODUCTION OF EXOPOLYSACCHARIDE BY PAENIBACILLUS HUNANENSIS STRAIN Y-22 ISOLATED FROM SOIL.
Author: Grenil Menezes and K. Aruna.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 189-199 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Paenibacillus hunanensis strain Y22, Exopolysaccharide
Abstract: An exopolysaccharide (EPS) producer was isolated from soil sample by primary screening. It was identified as Paenibacillus hunanensis strain Y22 on basis of biochemical studies and 16S rDNA gene sequence analysis. Semi-defined medium composition and other physical parameters viz, pH, temperature and time were varied for maximum exopolysaccharide production. The effect of variable carbon and nitrogen sources were also investigated.Paenibacillus hunanensis strain Y22 in semi-defined medium (SDM) Galactose (60 g/ L), Tween 80 (1ml/L), Yeast Nitrogen base (5g/L), Bacto-casitone (10 g/L), MgSO4 (0.1 g/L), NaNO3 (2g/L) pH-6.5 under shaker conditions (120rpm) for 6 days at 30? C produced 32 g/L of EPS. The other growth parameter like absence of Na-acetate, K2HPO4 and MnSO4 in SDM also showed remarkable increase in EPS yield. HPTLC analysis of EPS from Paenibacillus hunanensis strain Y22 showed presence of galactose, glucose, fructose and mannose. EPS produced by this organism also exhibited adhesive and emulsifying property, whereas Paenibacillus hunanensis strain Y22 exhibited antibacterial activity against some gram positive bacteria.
Location: TE 12 New Biology Building
Literature cited 1: Aguilera, Margarita, Monteoliva-Salnchez, Mercedes, Squrez Antonio, Guerra Victor, Lizama Catherine, Bennasar Antonio and Ramos-Cormenzana Alberto, 2001. Paenibacillus jamilae sp. nov., an exopolysaccharide-producing bacterium able to grow in olive-mill wastewater. International Journal of Systematic and Evolutionary Microbiology. 51: 1687-1692. Ash, C. Priest, F.G. and Collins, M.D. 1993. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus.Antonie Leeuwenhoek. 64: 253-260.
Literature cited 2: Ashtaputre, A.A. and Shah, A.K. 1995. Emulsifying property of a viscous exopolysaccharide from Sphingomonas paucimobilis. World Journal of Microbiology & Biotechnology. 11: 212-219. Azeredo, J. and Rosario, O. 1996. A New Method for Precipitating Bacterial Exopolysaccharides. Biotechnolgy Techniques. 5: 341-344.

ID: 59832
Title: ANTIOXIDANT ACTIVITY OF ACALYPHA ALNIFOLIA KLEIN EX WILLD LEAF EXTRACT.
Author: Vasthi Kennedy Evanjelene and Devarajan Natarajan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 185-188 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Acalypha alnifolia, Antioxidant.
Abstract: Antioxidants are vital substances which possess the ability to protect the body from damages caused by free-radical induced oxidative stress. A variety of antioxidants is found in dietary sources like fruits, vegetables and tea. The purpose of present study was to examine and determine in vitro antioxidant potential of methanol and aqueous extracts of Acalypha alnifolia, and to determine the IC50 values. The nitric oxide, super oxide and hydrogen peroxide radical scavenging assay were also analyzed. The best IC50 value was noticed in methanol extract of all tests i.e, scavenging assay were also analyzed. The best IC50 value was noticed in methanol extract of all tests i.e., scavenging activity of nitric oxide (> 1000mg/mL), hydrogen peroxide (550 mg/mL), super oxide (44.33 mg/mL) respectively. The overall results highlight that methanolic extract possess higher antioxidant activity than aqueous extract.
Location: TE 12 New Biology Building
Literature cited 1: Anandakumar, A.M., Paulsamy,S., Sathish Kumar, P. 2009. Preliminary phytochemical studies for the quantification of secondary metabolites of medical importance in the plant Acalypha fruiticosa Forssk. J. Appl Nat. Sci., 1 (1): 41-43. Anderson et al., 2001. The prevalence of Comorbid Depression in Adults With Diabetes,doi: 10.2337/diacare. 24.6.2001. Diabetes Care 24 (6): 1069-1078.
Literature cited 2: Balakrishanan, V., Prema, P., Ravindaran, K.C. and Philip Robinson,J. 2009. Ethnobotanical Studies among Villagers from Dharapuram Taluk. Global Journal of Pharmacology.3 (10): 08-14. Buyukokuroglu, M.E., Oktay, M. and Kufrevioglu, O.I. 2001. In vitro antioxidant properties of dantrolene sodium pharmacol. Res.44: 491-95.

ID: 59831
Title: PERFORMANCE OF IMMOBILIZED BACILLUS SUBTILIS SUBSP. SUBTILIS "RA-29" IN CONGO RED DECOLOURIZATION
Author: Rajesh Sawhney, Arun Kumar, Robina and Jayata
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 179-184 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Bacillus subtilis, Congo red, Immobilization, Calcium alginate, Reusability.
Abstract: Congo red, a carcinogenic diazo dye is discharged through industrial effluents in the environment. The removal of this hazardous dye from effluents is important for health and environment protection. In recent past, immobilization has come up as a promising biological tool. The present study highlights the immobilization of Bacillus subtilis sub sp. subtilis RA-29? on a suitable matrix and the potential of immobilized whole cells to decolourize congo red. The whole cell immobilized calcium alginate beads were stable for 7 days. The immobilized RA-29 exhibited cell density dependent decolourization. Maximum congo red decolourization (81.55%) was achieved at cell density of 30 ug/mm3 after 48 hr of incubation at 37? C under static condition. The calcium alginate immobilized ?RA-29? showed operational stability with efficient decolourization up to five cycles with minimum decolouriztion percentage falling to 61.11 %.The immobilized Bacillus subtilis could be commercially viable biotechnological tool for bioremediation of dye contaminated industrial effluents.
Location: TE 12 New Biology Building
Literature cited 1: Bryant, S.J, Nuttleman, C.R. and Anseth, K.S. 2000. Cytocompatibility of UV and visible light photoiniating systems on cultured NIH/ 3T3 Fibroblasts in vitro. Journal of Biomaterial Science Polymer Edition. 11: 439-457. Chang, J.S, Chou, C. and Chen, Y.S. 2001. Decolourization of azo dyes with immobilized Pseudomonas leteola. Process Biochemistry. 36 (8-9): 757-763.
Literature cited 2: Chen, K.C. Wu, J.I, Huang, C.C, Liang, Y.M. and Hwang, SzCJ. 2003. Decolourization of azo dye using PVA-immobilized microorganisms. Journal of Biotechnology. 101 (3): 241-252. Couto, S.R. 2009. Dye removal by immobilized fungi. Biotechnology Advances. 27: 227-235.

ID: 59830
Title: ANTIBIOTIC RESISTANCE PATTERN OF SOME CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS IN THE HOSPITALS OF AKOLA, MAHARASHTRA, INDIA.
Author: A.M.Banole and V.D. Nanoty.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 175-177 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antibiotic resistance, Staphylococcus aureus, Hospital, Vancomycin resistance.
Abstract: Antibiotic resistance pattern of 187 clinical isolates of staphylococcus aureus was studied. Maximum resistance was observed against viz., Ampicillin, Azolocillin, Clindamycin, Ciprofloxacin, Co-trimoxazole, Ofloxacin, Vancomycin, Nitrofurantoin and Ticarcillin also showed high percentage of resistance to it. All the isolates from Government hospital showed higher resistance to all antibiotics than that of charitable and private hospital.Vancomycin resistance was found between 80 to 95 % for isolates from government, private and charitable hospital.
Location: TE 12 New Biology Building
Literature cited 1: Amarnath Satish Kumar, 2003. The silent killer in hospital. Highlight the hidden perils of hospitals acquired infections. www.vasanthvisuals.com/hospital.html (The New Indian Express, Bangalore, April 7, 2003.) Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turk, M. 1996. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol. 45: 493-496.
Literature cited 2: CDC, Reduced susceptibility of Staphylococcus aureus to Vancomycin, 1997. MMWR. 46: 624-626. Chakravarty, A., Talwar, V. and Gupta, H. 1988. Antibiotic resistance pattern of Staphylococcus aureus with special reference to methicillin resistance strains. Indian J. Med. Res.87: 570-572.

ID: 59829
Title: SANITARY ANALYSIS OF SITARAAM JAJU SAGAR DAM, NEEMUCH, MADHYA PRADESH.
Author: Sonu Chouhan, Archana Pancholi and Ritu Vyas.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 167-173 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Coliforms, contamination, drinking water, pathogens, sanitation, total viable count.
Abstract: Diseases from dirty water and lack of sanitation kill more people every year than all forms of violence including war. Priority needs to be given to improving and developing the drinking water supplies that represent the greatest public health risk. Improving access to safe drinking water can result in tangible benefits to health. Every effort should be made to achieve a drinking water quality as safe as practicable. AIM: The present research was intended to obtain information about the sanitary situation of Sitaram Jaju Sagar Dam. The dam is the sole source of drinking water to the inhabitants of Neemuch. The Dam water is treated by Hingoria Water Treatment Plant through Flocculation by Alum, Rapid Sand Filtration and final chlorination by chlorine. This treated water is directly supplied to the community without any bacteriological testing; hence this study aims to evaluate the microbiological quality of water and comparison of the results with the National and International Standards for drinking water. METHODOLOGY: Water samples from the Dam, outlet of plant and Household tap were collected twice a month and analyzed for Total Viable Count, Total Coli forms and presence of water borne Pathogens, using Standard methods (APHA, 99). RESULTS & DISCUSSION: The results revealed that water samples from the dam, Hingoria plant and household tap failed to meet the standards, throughout the year and therefore are not acceptable for drinking. The bacterial isolates recovered from the samples are-V.cholerae, Salmonella, Shigella, P. aeruginosa, S. aureus, and E.coli. CONCLUSION: From the results, it can be concluded that the dam water is highly contaminated with fecal matter, domestic sewage, soil and agricultural runoff, effluents from septic system and is not suitable for drinking; moreover the Hingoria Treatment Plant is inefficient to treat such a highly contaminated water to make it suitable for drinking. RECOMMENDATIONS: Hingoria Treatment Plant must be upgraded and supplied water must be regularly assessed for the presence of coli forms at its source and consumer point areas before an outbreak of epidemic occurs.
Location: TE 12 New Biology Building
Literature cited 1: Aneja K.R. 2008. Experiments in Microbiology plant Pathology and Biotechnology. New Age International (P) Limited Publishers Ed 4: 361-368. A.P.H.A. 1999. Standard Methods for the examination of water and waste water. 21th ed. American Public Health Association, 1015. Fifteenth street, NW, Washington, DC.
Literature cited 2: A.P.H.A. 1995. Standard Methods for Examination of Water, Sewage and Industrial Wastes: 19th ed. American Public Health Association, Washington, U.S.A. APHA1998. Standard methods for the examination of water and wastewater. 20th ed. American Public Health Association, Washington, DC.

ID: 59828
Title: EVALUATION OF DIFFERENT MEDIA FOR FERMENTATIVE PRODUCTION OF BIOPIGMENTS USING YEAST CULTURES.
Author: Reeba Panesar, Paramjit S. Panesar and Manab B.Bera.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 161-166 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Yeast, Rhodotorula, Kinnow peel, Media, Biopigments.
Abstract: In the present investigation, six different media were evaluated for the production of bio pigments using two yeast strains. The synthetic media based on glucose was compared with kinnow peel based media supplemented with different ingredients. The various combinations of chemical constituents as well as salts were added to the media to increase the biopigment production and minimize the cost of production process. The comparative studies indicated that Rhodotorula sp. MTCC 1446 has better potential for pigment production from kinnow peel based media. Further, taro leaves can be good supplement for pigment production to improve the economics of the process. The comparison of extraction methods indicated that acetone-ether extraction method was found to be better method than acid extraction method.
Location: TE 12 New Biology Building
Literature cited 1: Ahmed, M.M., Nomani, M.S and Panda, B.P. 2009. Screening of nutrient parameters for red pigment production by Monascus purpureus MTCC 369 under submerged fermentation using placket-burmen design. Chiang Mai. J. Sci. 36: 104-109. Aksu, Z. and Eren, A.T. 2005. Carotenoid production by the yeast Rhodotorulla mucilaginosa: Use of agricultural wastes as a carbon source. Process Biochem. 40: 2985-2991.
Literature cited 2: Aksu, Z. and Eren, A.T. 2007. Production of carotenoids by isolated yeast of Rhodotorula glutinis. Biochemical Engg. J 35: 107-113. An, G.H., Jang, B.G. and Cho, M.H. 2001. Cultivation of the carotenoid-hyperproducing mutant 2A3N of the red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) with molasses. J. Biosci.Bioeng. 92: 121-125.

ID: 59827
Title: CANDIDAL BIOFILM: AN OUTLOOK ON THE MORPHOLOGICAL ASPECT AND FORMATION.
Author: Florida Primrose and Nithya Jagannathan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 155-159 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Adherence, Biofilm, Candida, Matrix, Invasion
Abstract: Candida is the dimorphic fungi which is the most common microbes present in the body. Biofilms are communities of microorganisms which are often attached onto a surface and enclosed in an extracellular polysaccharide matrix. The formation of a mature biofilm is based on the transition between the hyphae and the yeast forms of the candidal species, cell adhesion and the extracellular matrix formation. Biofilm formation is a major virulence factor in the pathogenecity of Candida, and Candida biofilms are difficult to eradicate because of their very high antifungal resistance. Candida can cause both superficial and systematic disease, and are now recognized as major agents of hospital-acquired infection. Numerous studies have been performed to determine the role of biofilm in infections. The current review focuses on the steps involved in biofilm formation, molecular characteristics and the factors which play a role in biofilm formation with a mention on the drug resistance.
Location: TE 12 New Biology Building
Literature cited 1: Alem, M.A. and Douglas, L.J. 2005.Prostaglandin production during growth of Candida albicans biofilms. J. Med Microbiol. 54: 1001-1005. Al Fattani, M.A and Douglas, L.J. 2006. Biofilm matrix of Candida albicans and Candida tropaicalis: chemical composition and role in drug resistance. J. Med Microbiol.55: 999-1008.
Literature cited 2: Andes, D., Nett, J., Oschel, P., Albrecht, R., Marchillo, K. and Pitula, A. 2004. Development and characterization of an in vivo central venous catheter Candida albicans biofilm model. Infect Immun. 72: 6023-6031. Bachmann, S.P., VandeWalle, K., Ramage, G., Patterson, T.F., Wickes, B.L., Graybill, J.R and Lopes-Ribot, J.L. 2002. In vitro of caspofungin against Candida albicans biofilms. Antimicrob Agents Chemother. 46 (11): 3591-3596.

ID: 59826
Title: COMPARATIVE EVALUATION OF ANTIMICROBIAL ACTIVITIES OF ROOT, STEM AND LEAVES OF HOLOPTELEA INTEGRIFOLIA AGAINST PATHOGENIC BACTERIA.
Author: Kavitha Alli and Lakshmi Narasu Mangamoori.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 145-154 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Haloptelea integrifolia, Zone of inhibition, Agar well diffusion method, Minimum inhibitory concentration, Phytochemical components, GC-MS analysis.
Abstract: Haloptelea integrifolia, a large spreading glabrous tree belonging to the family of ulmaceae is widely used in traditional medicine for treatment of cancer, gastritis, diabetes, wound healing and is known to possess many phytochemicals. The nature of phytochemicals present in the stem, leaf and roots were possess many phytochemicals. The nature of phytochemicals present in the stem, leaf and roots were analyzed with different solvents using GC-MS analysis and evaluated for antimicrobial activity. The antimicrobial activity of these extracts were checked against a range of gram positive and gram negative pathogens using agar well diffusion method. The minimum inhibitory concentration and minimum bactericidal concentration was determined by two-fold broth dilution method and compared against the standard antibiotics chloramphenicol. The antimicrobial activities were comparable to those obtained with standard antibiotics. Root extracts had exhibited highest antimicrobial activity followed by stem and leaves. Acetone extracts of root and ethyl acetate extract of stem had exhibited good activity against the pathogens tested. The GC-MS analysis also revealed phenols, carboxylic acids, esters and other phytochemical components for Holoptelea integrifolia, which assured that it has high antimicrobial activity against pathogens. This study clearly demonstrates that Holoptelea integrifolia has good antimicrobial properties and the root has better antimicrobial properties than leaves and stem. All extracts except the aqueous extract had significant broad-spectrum inhibitory activity. Our study is the first to present the evidence of better antimicrobial properties of root over stem and leaves.
Location: TE 12 New Biology Building
Literature cited 1: Bambhole, V.B. and Jiddewar, G.G. 1985. Anti-obesity effect of iris versicolor and Holoptelea integrifolia in rats. Sachitra Ayurved. 37: 557-561. Cohen, M.L. 1992. Epidemiology of drug resistance: implications for a post-antimicrobial era. Science. 257-(5073): 1050-5
Literature cited 2: Ellof, J.N. 1998. Which extractant shoud be used for the screening and isolation of antimicrobial components from plants? J. Ethnopharmacol. 60: 1-8. Graham, J.G., Quinn, M.L., Fabricant, D.S. and Fransworth, N.R.2000 Plants used against cancer-an extension of the work of Jonathan Hartwell. J. Ethnopharmacol. 73 (3): 347-377, ISSN 0378-8741, 10.1016/S0378-8741(00) 00341-X.

ID: 59825
Title: BIOPROSPECTING MICROORGANISMS OF MARINE ENVIRONMENT-A REVIEW.
Author: Ayona Jayadev and S.S Navami.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 137-143 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Bioprospecting, Bioactive compounds, Marine bacteria, Marine fungi, Marine viruses, Marne actinomycetes.
Abstract: Organisms associated with marine environments have greater potential produce hydrolyzing enzymes such as amylase, lipase, protease, chitinase etc. and hence the bio-prospecting for these compounds is of greater importance. Various screening approaches could be developed to increase the ease with which the microbial products can be retrieved. It can be expected that more efficient and effective application of microbial natural products will contribute to the fields of industries, pollution abatement processes and medicine.
Location: TE 12 New Biology Building
Literature cited 1: Abe, F. and Horikoshi, K. 2001. The biotechnological potential of piezophiles. Trends Biotechnol. 19: 102-108. Alain, K. and Querellou, J. 2009. Cultivating the uncultured: limits, advances and future challenges. Extremophiles 13: 583-594.
Literature cited 2: Allen, E.J.1914. On the culture of the plankton diatom Thalassiosira gravid Cleve in artificial seawater, F. Mar. Biol. Assicia, U.K. 10: 417-439. Amann, R.I, Ludwing, W. and Schleifer, K.H. 1995. Phylogenetic identification and insitu detection of individual microbial cells without cultivation., Microbiol Rev. 59: 143-169.

ID: 59824
Title: CLINICAL VALIDATION OF A WHOLE-CELL VACCINE FOR HAEMOLYTIC UREMIC SYNDROME.
Author: Divya Sasitharan and M.Masilamani Selvam
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 131-135 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Escherichia coli 0157: H7, Whole-cell, Vaccine, Clinical trial, Haemolytic uremic syndrome.
Abstract: Background: A major public health goal is to prevent Entrohemorrhagic Escherichia coli (EHEC)-induced diseases in human and the same can be met by the development of vaccine against the EHEC strains, such as Escherichia coli 0157:H7. Aim and Objective: To determine the efficacy of whole-cell vaccine prepared out of Escherichia coli 0157:H7, for having its pathogenicity lost, using laboratory animals such as rats. Samples such as ground beef, chicken intestine, raw milk and pasteurized milk was collected and analysed or the isolation and identification of Escherichia coli strain by standard microbiological techniques. Apart from general characterization, antibioticsensitivity tests were performed to determine antimicrobial resistance profiles. Conventional PCR amplification confirmed the presence of five selected virulence genes, namely Intimin (eae) Shigatoxin 1 (stx) 1, Shigatoxin (stx2), Hemolysin (hlyA) and Flagellar antigen (fliCh7) genes. One isolate was found to possess maximum number tested virulence genes and hence chosen for vaccine preparation. A whole cell formalin killed vaccine was produced out of selected isolate strain of Escherichia coli 0157: H7as per Jan Holmgren et al., (2003) protocol. Prepared vaccine was subjected to its efficacy test using rat models after obtaining proper ethical clearance. After challenging with ATCC and isolates, screening methods emphasized on studying the histopathology effects on test rats as well as on control rats (mortality as well as morbidity in liver and kidney) were done. Normal histology was seen in liver and kidney specimens of vaccinated and challenged rat groups as well as control uninoculated unvaccinated rat groups. However, toxic evidences were observed in liver as well as kidneys of rat groups which were unvaccinated and challenged with pathogenic strains. Vaccine is believed to have lost its pathogenecity as validated by animal studies as expected. However, this is just a preliminary confirmation before proceeding to clinical trials with human volunteers.
Location: TE 12 New Biology Building
Literature cited 1: Chik, H. Pai, Rhonda Gordon, R.T., Harry, V. Sims and Lawrence, E. Bryan, 1984. Sporadic cases of hemorrhagic colitis associated with Escherichia coli 0157:H7: Clinical, epidemiologic, and bacteriologic features. Annual Internal Medicine. 101 (6): 738-742. EL-Jakee, J., Moussa, E.I., Mohamed K.H.F. and Mohamed, G. 2009. Using molecular techniquies for characterization of Escherichia coli isolated from water sources in Egypt. Global Veterinaria. 3 (5): 354-362.
Literature cited 2: Evans, D.J. Jr, Evans, D.G., Opekun, A.R. and Graham, D.Y.1988. Immunoprotective oral whole cell vaccine for enterotoxigenic Escherichia coli diarrhea prepared by insitu destruction of chromosomal and plasmid DNA with colicin E2.FEMS Microbiol Immunol. 1 (1): 9-18. Gould, L.H., Demma, L., Jones, T.F., Hurd, S., Vugia, D.J., Smith, K., Shiferaw, B., Segler, S., Palmer, A., Zansky, S. and Griffin, P.M., 2009. Hemolytic uremic syndrome and death in persons with Escherichia coli 0157: H7 infection, food-borne diseases active surveillance network sites, 2000-2006. Clinical Infectious Diseases. 15 (10): 1480-1485.

ID: 59823
Title: EVALUATION OF ANTIBODIES RAISED AGAINST NATIVE AND RECOMBINANT ANTIGENS IN PLATE ELIS FORMAT FOR THE IMMUNOLOGICAL CHARACTERIZATION OF BURKHOLDERIA PSEUDOMALLEI CAUSATIVE ORGANISM OF MELIODOSIS.
Author: Archana Prakash, Sonia Arora, Duraipandian Thavaselvam, Ashu Kumar, Anita Barua and K.Sathyaseelan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 121-129 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Burkholderia pseudomallei, Melioidosis, ELISA, Bacteria Characterization.
Abstract: Meliodosis is an emerging infectious disease in India and the soil dwelling Gram-negative pathogen Burkholderia pseudomallei is the causative organism for this disease. The disease is endemic in Southeast Asia and northern Australia with highest percentage of melioidosis cases reported by Thailand. This disease is increasingly becoming an emerging disease in mostly coastal regions of India. In the present study antibodies raised against several native and recombinant antigens of Burkholderia pseudomallei was evaluated in plate ELISA format for the immunological characterization of clinical and environmental isolates. In all sixty nine whole cell native antigens of Burkholderia pseudomallei strains, Burkholderia related and other species species were tested with rabbit polyclonal antibodies raised against sonicated antigen of B. pseudomallei NCTC10274, mice anti-recombinant GroEL protein antigen, mice anti-cell envelope protein antigen, monoclonal antibodies raised against whole cell sonicated antigen and human Melioidosis sera from culture positive clinical case as positive control. The polyclonal antibodies raised against sonicted antigen of B.pseudomallei reference strain was found cross reactive with 20 other bacterial species and polyclonal antibodies raised against cell envelope antigen reacted with 5 other bacterial species. The polyclonal antibodies raised against recombinant GroEL protein cross reacted with 8 other bacterial species and the pooled human culture positive sera reacted with six bacterial species. All the polyclonal antibodies tested although had positive reaction with all the reference strains, clinical and environmental isolates of B. pseudomallei but cross reacted with other bacteria. The two monoclonal antibodies tested reacted selectively with the B. pseudomallei isolates and no reaction was observed with any of the other bacterial species tested. These results will be very useful in not only developing immuno assay for detection but also for rapid characterization of isolates.
Location: TE 12 New Biology Building
Literature cited 1: Cheng, A. C. and Currie, B.J. 2005. Meliodosis: epidemiology, pathophysiology, and management. Clin. Microbiol.Rev.18: 383-416. Cheng, A.C., O Brien, M., Freeman, K., Lum, G. and Currie, B.J. 2006. Indirect hemagglutination assay in patients with Melioidosis in northern Australia. Am. J. Trop. Med. Hyg. 74: 330-334.
Literature cited 2: Chenthamarakshan, V., Vadivelu J. and Puthucheary, S. D.2001. Detection of immunoglobulins M and G using culture filtrate antigen of Burkholderia pseudomallei.Diagn.Microbiol.Infect. Dis. 39: 17. Dharakul, T., Songsivilai, S., Anuntagool, N., Chaowagul, W., Wongbunnate, S., Intachote, P. and Sirisinha, S. 1997. Diagnostic value of an antibody enzymelinked immunosorbent assay using affinity-purified antigen in an area endemic for melioidosis. Am.J.Trop.Med.Hyg.56: 418-423.

ID: 59822
Title: EFFICACY OF ACCUMULATION ON HEAVY METALS FROM AQUEOUS SOLUTION USING WATER HYACINTH (EICHHORNIA CRASSIPES)
Author: I.Ashok, R. Balaji, S.Sakthivel and Sheela Sasikumar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 115-120 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Water hyacinth, Heavy metals, metal contamination, Echhornia crassipes, Phytoremediation, Physico chemical.
Abstract: The present study aims to find out the efficiency of water hyacinth in accumulation and fast absorption of all heavy metals from the aqueous solution. This study infers that water hyacinth plays a vital role in phytoremediation. Eichhornia crassipes (water hyacinth) has been tested for removal of four important heavy metals chromium (Cr) lead (Pb) cadmium (Cd) and zinc (Zn) from metal solution. The plant was grown at four different 20 ppm metal solutions in laboratory condition. The results shows significant removal (up to 63 %) of heavy metals during 10th day when compared to other different time point 1st day, 5th day, 8th day treated groups. Average physico chemical composition of the water was also analyzed at different time point 1st day, 5th day, 8th day and 10th day treated group showed a significant difference when compared to control and the 10th day treated group was highly significant. From our results we conclude Eichhornia crassipes has the efficiency to with stand all metal contamination of the selected heavy metals and it can be used for removal of heavy metals from metal polluted water bodies.
Location: TE 12 New Biology Building
Literature cited 1: Bastviken, S.K., Eriksson, P.G., Premrov, A. and Tonderski, K. 2005. Potential denitrification in wetland sediments with different plant species detritus. ECOL. Eng. 25: 183-190 Chua, H. 1998. Bio-accumulation of environmental residues of rare earth elements in aquatic ora Eichhornia crassipes, (Mart) Solms in Guangdong Province in China.The Science of the Total Environment. 214: 79-85.
Literature cited 2: Chung, A.K.C, Wu, Y., Tam, N.F.Y. and Wong, N.F.Y. 2008. Nitrogen and phosphate mass balance in a sub-surface ow constructed wetland for treating municipal wastewater. Ecological Engineering. 32: 81-89. Cordes, KB., Mehra, A., Farago, M.E. and Banerjee, D.K. 2000. Uptake of cd, Cu, Ni and Zn by the water hyacinth, E ichhornia crassipes (Mart) Solms from pulverized fuel ash (PFA) leachates and slurries. Environ. Geochem. Health. 22: 297-316.

ID: 59821
Title: RESPONSE SURFACE METHODOLOGY FOR OPTIMIZATION OF BIOSURFACTANT PRODUCTION FROM BACILLUS SP. VITPGMB ISOLATED FROM MARINE WATER.
Author: Meenakshi Singh Grusha, Piyush Jain, L. Karthik, G. Kumar and K.V.Bhaskara Rao.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 109-113 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Biosurfactant, Lipase activity, Hemolytic activity, Emulsification index, MEOR, Response Surface Methodology.
Abstract: The aim of the present study was to isolate microorganism that produces biosurfactant and to optimize its production. Lipase and hemolytic activity were performed as primary screening procedures. Three potential isolates (GMP 1 to GMP3) were subjected to secondary screening by Drop collapsing method, Oil displacement method and Emulsification index. A decrease in surface tension at oil-water interface was recorded. Microbial Enhanced Oil Recovery (MEROR) was performed and oil elution recorded from GMP1 and GMP2 was 2.0 mL and 2.2 mL respectively. GC-MS analysis of the extract from GMP2 revealed the presence of surface active compounds. Response Surface Methodology (RSM) was used to study the interactions between various factors and it was found that a pH=8.91, temperature =25.66 ? C, peptone-4.04 g/L, yeast extract-3.09 g/L and concentration of oil-0.52 % were optimal for production from GMP2. The isolate was identified as Bacillus sp. VITPGMB.
Location: TE 12 New Biology Building
Literature cited 1: Banat, I.M., Franzetti, A., Gandolfi, I., Bestetti, G., Martinotti, M., et al.2010. Mcrobial biosurfactants production, applications and future potential. Appl.Microbiol. Biotechnol. 87 (2): 427-444. Bognolo, G. 1999. Biosurfactants asemulsifying agents for hydrocarbons. Colloid Surf. A Physicochem. Eng. Aspect. 152: 41-52.
Literature cited 2: Christofi, N. and Ivshina, I.B. 2002. Microbial surfactants and their use in field studies of soil remediation. J. Appl. Microbiol. 93 (6): 915-929. Clark, R.B. 1999. Marine Pollution.Oxford University Press, Fourth edition, 161.