Enter records

ID: 59850
Title: ISOLATION AND CHARACTERIZATION OF RHIZOBIAL ISOLATES FROM THE RTHIZOSPHERIC SOIL OF AN ENDANGERED PLANT MEIZOTROPIS PELLITA.
Author: Pankaj, Tapan Kumar Nailwal, Lalit Singh and Amit Panwar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 301-306 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Meizotropis pellita, PGPR, Rhizosphere, nif genes.
Abstract: Meizotropis pellita (vern, Patwa) an endangered plant species found on the three sites in Patwadang 1r Nainital Uttarakhand. Meizotropis pellita is an angiospermic plant which belongs to family fabaceae. Most of the fabaceae family plants fix atmospheric nitrogen. Patwa belong to this family, so rhizobacterial spp. from its rhizospheric soil were isolated and characterized. This study focuses on the important role of Rhizobacteria associated with the Rhizospheric soil of Meizotropis pellita. By the biochemical properties of the Rhizobial isolates it is proved that these Rhizobial isolates have potent plant growth promoting abilities (PGPR). PGPR properties of Rhizobial isolates is based on their phosphate solubilisation property. All strain showed phosphate solubilisation property. Seven different rhizobacterial strains of bacteria were isolated from the rhizosphere of Patwa plant. Biochemical characterization was done for their identification, and molecular characterization authenticated that the isolated bacteria can fix atmospheric nitrogen and this may have PGPR activity. Nif genes expresses and produce nitrogenase enzyme which is mainly responsible for nitrogen fixation. In this study we used PCR amplifications of nif genes present in the isolated bacterial strains. All seven isolate showed nif gene amplification. This is the first study associated with the Rhizospheric region of the M. pellita plant.
Location: TE 12 New Biology Building
Literature cited 1: Ahmad, F., Ahmad, I. and Khan, M.S. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res. 163: 173-81. Bazzicalupo, M. and Fani, R. 1995. The use of RAPD for generating specific DNA probes for microorganisms. In: Methods in Molecular Biology Diagnostic Protocols: PCR and Other Nucleic Acid Methods. Clapp J. (Tatowa, N.J., ed) Humana Pres Inc. pp. 155-175.
Literature cited 2: Cattelan, A.J., Hartel, P.G., and Fuhrmann, J.J. 1999. Screening for plant growth promoting rhizobacteria to promote early soybean growth. Soil. Sci. Soc. Am. J. 63: 1670-1680. Graham, P., Sadowsky, M., J., and Keyser, H.H. 1991. Proposed minimal standards for the description of new genera and species of root and stem nodulating bacteria. Int J Syst Bacteriol. 41: 582-587.

ID: 59849
Title: SCREENING THE POST DINNER BLOOD PRESSURE CHANGS IN HEALTHY ADULTS BETWEEN THE AGE GROUP OF 19-22.
Author: S.M. Sathak Sameer, P. Manigandan, K. Anusha, M.Chandran and Sekarbabu Hariram
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 299-300 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Vegetarian, Non vegetarian, Dosage, Monitoring.
Abstract: The present study is designed to explore the changes in blood pressure in healthy volunteers (male & female) with the age group of 19-22 after vegetarian/ Non vegetarian dinner. The selected subjects were screened for blood pressure at 5 different periods such as pre, post dinner, zero hour, 4.0 A.M and 7.0 A.M. Volunteers of either sex divided in to 2 groups such as vegetarian and non vegetarian. The results indicates that there is a considerable reduction in blood pressure after dinner both in vegetarian male and female till 4 A.M but in males there is a marginal increase in blood pressure. Regarding Non Vegetarian dinner males shows highly fluctuating blood pressure till 4 A.M whereas in females it shows a steep increase in blood pressure till 4.A.M. The results indicate the significant difference may be due to digestive functioning and the influence of endocrines on the physiology. This type of study will help us to design the dosage schedule of drug for various ailments. It also forms a base for the further researchers to probe the reason for fluctuation in blood pressure.
Location: TE 12 New Biology Building
Literature cited 1: Barr, W. 2000. Lunacy revisited. The influence of the moon on mental health and quality of life. Journal of Psychosocial Nursing and Mental Health Service. 38: 28-35.
Literature cited 2: Zimecki, M. 2006. The lunar cycle. Effects on human and animal behavior and physiology. Pubmed Gov. 60: 1-7.

ID: 59848
Title: COMPARAQTIVE ANALYSIS OF BACTERIOCINS PRODUCED BY PSEUDOMONAS AERUGINOSA AND BACILLUS BREVIS.
Author: V.Jeyanthi Kumari.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 295-298 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Bactriocin, Bacillocin, Pyocin.
Abstract: An attempt is made to comparatively analyse the effectiveness of bacteriocins produced by the indigenous soil bacteria namely Pseudomonas aeruginosa and Bacillus brevis against selective pathogens such as Staphylococcus aureus, Streptococcus pyogens, Enterococcus faecalis and Corynebacterium diptheriae by measuring the zone of inhibition. The bacteriocins are bacillocin of B.brevis and pyocin of P. aeruginosa. Both the bacteriocins effectively acted against the pathogens and comparatively pyocin of P.aeruginosa gave better results than bacillocin of B. brevis. But the effectiveness of bacillocin was particularly greater against Corynebacterium diptheriae (20 mm) than the effectiveness of pyocin (10 mm). Also the determination of bacteriocin production in growth curve revealed that the production of bacteriocins started during early exponential growth phase and continued till last stationary phase. Maximum production of bacillocin and pyocin was found at 11 hours of incubation.
Location: TE 12 New Biology Building
Literature cited 1: Bizani, D. and Brandelli. 2000. A. Characterization of a bacteriocin produced by a newly isolated Bacillus sp strain 8A. J. Appl. Microbiol. 93: 512-519. Buckenhuskes, H.J. 1993. Selection criteria for lactic acid bacteria to be used as a starter culture for various food commodities. FEMS Microbiol. Rev. 12: 253-272.
Literature cited 2: Cleveland, J., Montville, J.T., Ingolf, N. Chikindas, M.L. 2001. Bacteriocins-safe natural antimicrobial agent for food preservation. Int. J. Food Microbiology. 71: 1-20. Cotter, D.D., Hill, C. and Ross, R.P. 2006. Class distinction for bacteriocins. Nature Reviews Microbiology. 4777-788.

ID: 59847
Title: ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF DUNALIELLA BARDWIL.
Author: A.Jenitta and S.Jayasuriya.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 285-293 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Dunaliella bardwil, Antioxidants, Radical scavenging activity, Polyphenolic compounds.
Abstract: An ideal antioxidant should be readily absorbed and quench free radicals, and chelate redox metals at physiologically relevant levels. The present study is focused on the antioxidant and free radical scavenging properties of Dunaliella bardawil. The objective of the present study is to evaluate the antioxidant activity of D. bardawil using seven methods viz., DPPH radical quenching method, H2O2 scavenging assay, No radical scavenging assay, reducing capacity assay and FTC method, TBA method and Total phenolic content.Ascorbic acid, ?-Tocopherol and vitamin C were used as standards. The methanolic extract of D.bardawil showed strong DPPH radical scavenging activity of 86.72 ? 0.33 % value with IC50 value of 2.25 ? 0.11 ?g/mL and H2O2 scavenging activity of 94.24 ? 1.33 % with IC50 value of 3.88 ? 0.67 ?g/mL, No radical scavenging activity with 63.55? 0.52 % with IC50 value of 3.22 ? 0.28 ?g/mL. The Dunaliella extract with concentrations higher than 4 mg/mL showed a higher reducing power than alpha-tocopherol and BHA.A volume of 800 ?L of 2.5 % linoleic acid in 99.8 % ethanol in a total volume of 20 mL, was found to produce the highest absorbance readings and both showing a slightly reduced absorbance after the 8th day. Maximum generation of lipid hydroperoxides was achieved by incubation at 37? C (body temperature). In Green phase the contents of phenolic compounds (mg/g) in aqueous extracts of D. bardawil was found 482. 18 ? 12.30 mg GAE /g dry wt and in red phase 525 ? 30.19 mg GAE /g dry wt. Dunaliella showed relatively fair antioxidant potential according to FTC and TBA method but comparatively low DPPH radical scavenging activity. From all these observations it can be concluded that, the algal extracts with high level of polyphenolic compounds and ? carotene in Dunaliella show excellent antioxidant activity in vitro systems.
Location: TE 12 New Biology Building
Literature cited 1: Abalde, J.J., Fabregas and Herrero, C. 1991. b-carotene, vitamin C and Vitamin E content of the marine microalgae Dnaliela tertiolecta cultured with different nitrogen sources. Bioresource Tech. 38: 121-5 Balakrishnan, N., Panda, A.B., Raja, N.R., Srivastava, A. and Prathani, R. 2009. The evaluation of nitric oxide scavenging activity of Acalypha indica Linn Root. Asian.J. Research Chem 2: 148-150.
Literature cited 2: Benzie, I.F.F. and Strain, J.J. 1999. Ferric reducing antioxidant function of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology.299: 15-27. Cai, Y., Luo, Q. and Sun, M. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74: 2157-2184.

ID: 59846
Title: EVALUATION OF PHYTOTOXICITY OF METALIC NANOPARTICLES AGAINST AMARANTHUS RETROFLEXUS.
Author: A.Aruna, S.Karthick Raja Namasivayam and P.Sarath Chandu.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 279-284 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Phytotoxicity, Metallic nanoparticles, Amaranthus retroflexus
Abstract: Nanotechnology is currently employed as a tool to explore the darkest avenues of medical sciences in several ways like imaging, sensing, targeted drug delivery, gene delivery systems and artificial implants. Based on enhanced effectiveness, the new age drugs re-nanoparticles of polymers, metals or ceramics, which can combat conditions like cancer and fight human pathogens like bacteria. Developmental phytotoxicity of NMS is a critical knowledge gap because nanoparticles entering wastewater streams may predominantly be incorporated into sewage sludge and applied to agricultural fields. In the present study, phytotoxicity of chemogenic metallic nanoparticles such as silver, nano zero valent iron and zinc oxide against common weed against Amaranhus retroflexus. Nanoparticles were synthesized by chemical reduction of metal precursor with reducing agent and the synthesized nanoparticles characterized by scanning electron. Among the different nanoparticles treatment, zinc oxide nanoparticles showed distinct herbicidal activity at all the tested concentration followed by silver. The activity of the nano particles was at 30 ?C followed by 40 ? C. Chlorophyll content was highly reduced in all the tested concentration. Phyllosphere microbial population was not recorded in all the nanoparticles treatment.
Location: TE 12 New Biology Building
Literature cited 1: Ahmad, A.P. Mukherjee, S. Senapati, D. Mandal and Sasqv, M. 2005. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf. 8.28: 313-318. Albrecht, M.A. and Raston, C.L. 2006. Green Chemistry. 8: 417-432.
Literature cited 2: Braga, L.C., A.A., Leite, K.G. Xavier, J.A. Takahashi, M.P Bemquerer and E. Chartone Souza. 2005. Can J. Microbiol. 51: 541-547. Ganesh, S. and S. Karthick Raja Namasivayam , 2012. Biofilm Inhibitory Effect of Chemogenic Nano Zerovalent Iron Against Biofilm of Clinical Isolate of Staphylococcus aureus. Asian Journal of Chemistry. 24: 5533-5535.

ID: 59845
Title: : EFFECT OF DIFFERENT FACTORS ON THE PROTEOLYTIC ENZYMES ACTIVITY OF LACTOBACILLUS.
Author: P.Burde and A.O.Ingle
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 275-278 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Casein, Protease, Lactobacillus, Sodium chloride, D-glucose.
Abstract: Lactobacilli are isolated from curd samples. They also form part of mouth and alimentary tract of man. Proteolytic system in bacteria contribute to hydrolyse milk proteins and support for their ability to grow in milk and milk products. Casein is a family of phosphoproteins commonly found in mammalian milk. Casein hydrolyzing activity of the isolated bacteria is pH dependent. Also the separately supplemented sodium chloride 0.3 %, glucose 0. 5 % and maltose 0.2 % is found to increase the proteolytic activity significantly.
Location: TE 12 New Biology Building
Literature cited 1: Chouyyok, W., Wongmongkol, N., Siwarungson, N. and Prichnont, S. 2005. Extraction of alkaline protease using an aqueous two phase system from cell free Bacillus subtilis TISTR 25 fermentation broth. Process Biochemistry. 40: 3514-3518. Gupta, R., Beg O.K. and Lorenz, P. 2002. Bacterial alkaline protease: Molecular approaches and industrial application. Applied Microbial Biotechnology. 59: 15-32.
Literature cited 2: Ljungh, A.B., Wadstrom, A., Torkel (eds). 2009. Lactobacillus Molecular Biology, From genomics to probiotics. Caister Academic Press. Makarova, K., Slesarey, A., Wolf, Y., Sorokin, A., Mirkin, B., Koonin, E., Pavlova, N. 2006. Comparative genomics of lactic acid bacteria. Proc. Natl.Aca. Sci. USA.

ID: 59844
Title: BIODEGRADATION OF ACID BLUE 113: KINETIC ANALYSIS AND PLACKETT BURMAN SCREENING DESIGN FOR MEDIUM COMPONENTS
Author: A.Karunya, C.Valli Nachiyar, Swetha Sunkar and S. Anuradha Jabasingh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 267-273 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Consortium, Biodegradation, AB 113, Kinetics, Screening.
Abstract: Four different bacterial strains have been isolated from dye containing effluent and are used for the construction of an aerobic consortium CN-1 along with Pseudomonas aeruginosa CLRI BL22 obtained from CLRI. These bacteria were identified as Citrobacter freundii (2 strains), Moraxella osloensis and Pseudomonas aeruginosa using 16 S r RNA analysis. The organisms constituting the bacterial consortium CN-1 have started to degrade AB 113 in their middle lag phase. They exhibited difference only in the rate in which they carry out the dye degradation. The consortium CN-1 showed gradual increase in the rate of degradation which started in the middle lag phase and reached a maximum by 10h following first order reaction with the rate constant, k1 value being 0.152 h-1. With respect to growth, the consortium showed an extended log phase before it reached its stationery phase by 24h. Screening for medium components using Plackett Burman Screening Design indicated dye concentration having larger effect followed by temperature and ammonium nitrate concentration. It can be seen with low concentration of dye and low concentration of glucose, dye decolorization by the consortium was predominating, provided temperature and ammonium nitrate concentration were held high with pH maintained at 9.
Location: TE 12 New Biology Building
Literature cited 1: Acuner, E., and Dilek, F.B. 2004. Treatment of tectilon yellow 2G by Chlorella vulgaris. Process Biochemistry. 39: 623-631. Chang, J.S., Chou, C., Lin, P.J., Ho, J.Y. and Hu, T.L. 2001. Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola. Water Research. 35: 2841-2850.
Literature cited 2: Cudgar, R.J. 1985. ' Azodyes ' . In Kirk-othmer Encyclopedia Of Chemical Technology vol.3 Kroschwitz, J.I (Ed)., 4th Edition, Wiley, New York. Pp 821-875. Copley, S.D. 2009. Evolution of efficient pathways for degradation of anthropogenic chemicals. Nature Chemical Biology. 5: 559-566.

ID: 59843
Title: ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF HIGH SALT TOLERANT BACTERIA WITH CMCase ABILITY.
Author: A.E. Ekram, M.P.E. Shaekh, I.Sarker, S.Zaman, Z.Ferdousi, S.M. Shahik, M.S. Islam, M.S. Noore and A.S.M.H.K. Chowdhury.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 259-266 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: CMCase activity, Penaeus monodon, Salt tolerance, Metabolism
Abstract: A single bacterial colony was isolated by plating the aseptically separated crushed-cuticle sample of Penaeus monodon on an agar solidified LB medium, supplemented with 10 g/ 100 mL salt concentration. The bacterium was found to be gram positive, non-motile, aerobic and lactose non-fermenting through morphological and biochemical tests. The optimum culture condition of the isolate was at pH 7.5 with temperature of 30 ? C. The bacteria showed the ability of utilizing arabinose, sucrose, maltose, fructose as carbon sources and viable cell count was 489 x 107 CFU /mL. Isolated bacterium showed citrate metabolism ability, the ability of breaking down the amino acid tryptophan into indole and no catalase activity. The bacterium was resistant to amoxicillin, penicillin and vancomycin and the MIC value against penicillin was 100 mg/ mL. The bacterial colony was also specially characterized for CMCase production activity. The molecular weight of isolated bacterial genomic DNA was above 10 kb with not much smearing. High salt tolerance ability up to 12 g/ 100 mL medium was observed while culturing on different salt concentrations. Purpose of the study was to screen out a potential source of salt concentrations. Purpose of the study was to screen out a potential source of salt tolerant gene with CMCase activity.
Location: TE 12 New Biology Building
Literature cited 1: Amina, A. and Mahgoub, H.S.A.M. 2011. Salt Inducible Proteins and Conjugal Gene Transfer of Halotolerant Staphylococcus Isolated From Salinity Soil. Egypt. J. Genet. Cytol. 40: 263-280. Andersen, P.A., Kaasen I., Styrvold, O.B., Boulnois, G. and Trom, A.R. 1988. Molecular cloning, physical mapping and expression of the bet gene governing the osmoregulatory choline-glycine pathway of Escherichia coli. J. Gen. Microbio1.134: 1737-1746.
Literature cited 2: Annamalai, N., Thavasi, R., Vijayalakshmi, S. and Balasubramanian, T. 2011. A novel thermostable and halostable carboxymethylcellulase from marine bacterium Bacillus licheniformis AU01. World J Microbiol Biotechnol. 27: 2111-2115. Bashan, Y., Moreno, M. and Troyo, E., 2000. Growth promotion of the seawater-irrigated oilseed halophyte Salicornia bigelovii inoculated with mangrove rhizosphere bacteria and halotolerant Azospirillum spp. Biol Fertil Soils. 32: 265-272.

ID: 59842
Title: CARBON DIOXIDE FIXATION BY CO-CULTURE GREEN MICROALGAE THROUGH OVERALL VOLUMETRIC MASS TRANSFER COEFFICIENT (KL A) OF CARBON DIOXIDE IN CLOSED SYSTEM.
Author: Astri Rinanti, Edwan Kardena, Dea Indriani Astuti and Kania Dewi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 253-258 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: CO2 removal, Biomitigation, CO2 fixation, Volumetric mass transfer coefficient, Co-culture.
Abstract: Culture conditions are very important to CO2 bio-fixation but its implementation is limited by the poor mass transfer efficiency. The three green microalgae, i.e. Chlorella, Scenedesmus obliquus, and Ankinstrodemus sp were cultured as co-culture in a vertical bubble photobioreactor as closed system, with 160 ?m size diameter of pore membrane sparger to explore the possibility of coupling CO2 biofixation affected the carbon mass transfer process. In this study, overall volumetric mass transfer coefficient (KL a) of CO2 were developed with operational culture conditions at temperatures (? C) of 30, light intensities (lux) of 4000 and photoperiodism (light/dark; hour) of 16/8, with various high concentration (%) CO2 supplied from the bottom of photobioreactor were 0, 2, 5, 7. The result showed that the highest KL a value (h-1) was 0.1419 5% pure CO2 supplied, gas flow rate 8 L/ min during 12 days experiment. While at this condition, the maximum dried biomass (g/mL) was 2.7 (? = 0.38) at 5 % pure CO2. It could be concluded that CO2 removal efficiency (%) affected by the KL a value, whereas the highest CO2 removal efficiency (%) of 49.02 occurred when the KL a value was highest.
Location: TE 12 New Biology Building
Literature cited 1: Becker, E.W. 1995. Microalgae: Biotechnology and Microbiology. Cambridge University Press, Cambridge. Carvalho, A.P., Meireles L.A. and Malcata, F.X. 2006. Microalgal reactors: a review of enclosed system design and performances. Journal Biotechnology Progress. 6 (22): 1490-1506.
Literature cited 2: Contreras, Edgardo M. 2007. Carbon dioxide stripping in bubbled column. Journal of Industrial Engineering Chemical Resource, American Chemical Society. 46: 6332-6337. de Morais, M.G. and Costa, J.A.V. 2007. Biofixation of carbon dioxide by Spirulina sp. and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor. Journal of Biotechnology, 129: 439-445.

ID: 59841
Title: AN INVESTIGATION OF ENZYMATIC KINETIC RESOLUTION OF RACEMIC CLOPIDOGREL.
Author: Yaseer Gaber, Magdy Ali Amin and Rajni Hatti-Kaul.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 247-251 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: None
Abstract: This paper presents a biocatalysis based approach for asymmetric hydrolysis of Clopidogrel, one of the most effective antiplatelet aggregators. Instead of the conventional ?wet lab ?screening method, we used an advanced chemical information retrieval tool, Scifinder ?, to find suitable enzyme candidate (s) for the asymmetric hydrolysis. Scifinder search for reactions similar to the target reaction retrieved three potential hits: horse liver esterase, chemotrypsin and Candida rugosa lipase. Among the three, horse liver esterase was experimentally found to hydrolyse the correct isomer ? of the racemic clopidogrel. Investigation of the effect of different water miscible co-solvents showed DMSO to provide the highest enantiomeric excess (ee) value of 20 % at 43 % conversion of the racemic ester, while a dicyanamide based ionic liquid as a co-solvent showed 12 % ee. This article demonstrates the potential of the chemical databases as possible rapid, resource-efficient tool to find an appropriate selective biocatalyst.
Location: TE 12 New Biology Building
Literature cited 1: Arroyo, M., Torres-Guzman, R., de la Mata, I., Castillon, M.P. and Acebal, C. 2000. Enzyme Microb. Technol., 27: 122-126. Busto, E., Gotor-Fernandez, V. and Gotor, V. 2006. Nat. Protoc., 1: 2061-2067.
Literature cited 2: Chen, C.S., Fujimoto, Y., Girdaukas, G., and Sih, C.J. 1982. J. Am. Chem. Soc., 104: 7294-7299. Chiarello, J.F. Buckwalter, B.L. and Barden, T.C., 2002. Process for the preparation of chiral insecticidal and acaricidal 1, 4-diaryl-2-fluoro-2-butenes via enzymatic hydrolysis of (4-chlorophenyl) cyclopropylethanoic acid methyl ester using esterase. Basf Aktiengesellschaft, Germany p. 75 pp.

ID: 59840
Title: USE OF ANTIBIOTIC AND PROBIOTIC CONTROLLING WATER QUALITY, GROWTH AND SURVIVAL OF SHRIMP LARVAE PENAEUS MONODON FABRICIUS.
Author: Patang.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 241-245 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Shrimp, Antibiotics, Probiotics, Growth and survival.
Abstract: This study is aimed to determine the role of the use of antiobiotics and probiotics in controlling water quality maintenance, growth and survival of shrimp larvae. This study was an experimental study with application of 1 ppm Oxytetracyclin antibiotic and probiotic bacillus at a dose of 0.75 ppm, 1 ppm and 1.25 ppm. Test animals used were shrimp larvae PL-5 which were maintained in 60 L plastic container volume and 9 days after maintenance carried out sampling and day 14 in the total harvest. Biological study of shrimps were tested include growth and survival. Results showed media for maintenance of water quality such as temperature, salinity, pH, oxygen ppm, NH3, nitrite, nitrate, and BOT has a value that can be tolerated for the life of shrimp larvae. Furthermore, growth at the end of the study showed that the highest growth was obtained on treatment B 0.128 g, followed by 0.089 g treatment A, treatment C was 0.074 g and the lowest growth in the D treatment (control ) of 0.010 gr. While the highest survival rate of shrimp test animals highest in treatment C was 84.8%, followed by 78.6 % D treatment, treatment A was 78. 4 % and 77.2 % for treatment B.
Location: TE 12 New Biology Building
Literature cited 1: Ahmad, T. 1988. Farmed shrimp. Bunga Rampai. Coastal Aquaculture Research Centers, Maros. Asni, 2001. Analysis of Bacterial Pathogens in Water, Sediment and Dara clams (Anadara corne) in the waters of the Gulf of Pare-pare. Thesis. Graduate Program Hasanuddin University, Makassar.
Literature cited 2: Atmomarsono, M., Madeali, MI., Tompo, A. and Mulaini, 1993. Infectious bacteria in Pond Water Tiger Shrimp in South Sulawesi. Research Institute for Coastal Aquaculture, Maros. Bakhtiar, 2004. Effectiveness of the use of antibiotics for Disease Control Bacteria Vibrio harveyi in Tiger Shrimp Post larvae of Penaeus monodon Fabricius. Thesis. Graduate Program. Hasaniddin University. Makassar.

ID: 59839
Title: LIPID PRODUCTIVITY IN CHLORELLA PYRNOIDOSA UNDER NITROGEN STARVATION: ENERGY EVALUATION OF AN INTERNALLY ILLUMINATED BUBBLE COLUMN PHOTO BIOREACTOR.
Author: Rajasri Yadavalli, Ramgopal Rao and C.S Rao
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 233-239 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Internally Illuminated Bubble column Photobioreactor, Nitrogen starvation, Lipid productivity, Energy input, Chlorella pyrnoidosa.
Abstract: The effect of nitrogen starvation on the biomass and lipid productivities of Chlorella pyrenoidosa was studied in an internally illuminated bubble column photobioreactor (IIBCPBR) designed on the basis of energy efficiency. Algal culture was grown in the inside column with a culture volume of 4.5 L providing illuminated surface area of 0.1219 m2. The biomass growth rate and total lipid content were 3.12 g/L and 0.482 g/g, respectively, under nitrogen starvation after 8 days of cultivation period. Higher biomass productivity per total energy input (0.57 g W-1 day-1 ) and lipid productivity per total energy input (0.273 g W-1 day-1) were achieved with the IIBCPBR design under nitrogen starvation compared to other alternative designs reported in literature. The proposed IIBCPBR design can potentially reduce the energy input in algal cultivation by as much as 40 % compared to other bubble column photobioreactors.
Location: TE 12 New Biology Building
Literature cited 1: Bligh, E.G. and Dyer, W.J. 1959. A rapid method of total lipid extraction and purification. Can .J. Biochem. Physiol. 37: 911-917. Chiu, S.Y., Kao, C.Y., Chen, C.H., Kuan, T.C., Ong, S.C. and Lin, C.S. 2008. Reduction of CO2 by a high-density culture of Chlorella sp. in a semi continuous photobioreactor. Bioresour Technol. 99: 3389-3396.
Literature cited 2: Hulatt, C.J. and Thomas, D.N. 2011. Productivity, carbon dioxide uptake and net energy return of microalgal bubble column photobioreactors. Bioresour. Technol. 102: 5775-5787. Illman, A.M., Scragg, A.H. and Shales, S.W. 2000. Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb.Technol. 27: 631-635.

ID: 59838
Title: CHARACTERISTICS OF PALM OIL MILL EFFLUENT (POME) IN AN ANAEROBIC BIOGAS DIGESTER.
Author: B. Nurul Adela, N. Muzzammil, S.K. Loh and Y.M.Choo.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 225-231 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Palm oil mill effluent, Anaerobic digestion, Biogas, Microbial population.
Abstract: Palm oil mill effluent (POME) is generated during the palm oil milling process. The wastewater, POME undergoes anaerobic digestion in which biogas is emitted freely or captured as energy. Much research has been conducted to generate biogas from POME but little was focused on the microbial characteristics of the POME which is important to gauge the efficiency in POME treatment. In this study, microbial characterization of POME from an anaerobic digester system operating at mesophilic temperature (34.29 ? 0.8 ? C) was conducted. The system consisted of two 15-m height digester tanks and POME at three different levels-each having a height of 5 m was collected. The gram staining properties of the isolated bacteria at each level were determined via morphological and microscopic studies. It was found that the lowest part of the first tank receiving the incoming effluent exhibited the highest microbial population (1.16 x 106 CFUmL-1), thus facilitating anaerobic digestion of POME for biogas generation. Besides, volatile fatty acid (VFA) decreased from the bottom to the top of the tanks indicating that the system was able to create and maintain a stable and beneficial environment for the active microbial digestion in order to treat POME and to generate biogas efficiently.
Location: TE 12 New Biology Building
Literature cited 1: Appels, L., Baeyerns, J., Degreve, J. and Dewil, R. 2008. Principle and potential of the anaerobic digestion of waste-activated sludge. Progress in Energy and Combustion Science. 34 (6): 755-781. Borja, R. and Banks, C.J. 1995. Comparison of an anaerobic filter and an anaerobic fluidized bed reactor treating palm oil mil effluent. Process Biochemistry. 30 (6): 511-521.
Literature cited 2: Borja, R., Banks, C.J and Salnchez, E., 1996. Anaerobic treatment of palm oil mill effluent in a two stage up-flow anaerobic sludge blanket (USAB) system. Journal of Biotechnology. 45: 125-35. Choorit, W. and Wisarnwan, P. 2007. Effect of the temperature on the anaerobic digestion of palm oil mill effluent. Electronic Journal of Biotechnology. 10: 376-85.

ID: 59837
Title: INTEGRATED WEED MANAGEMENT IN CARROT (DAUCUS CAROTA L)
Author: K.Chaitanaya, S. Amarender Reddy, A.V.N. Lavanya and S. Srinivasa Raju.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 221-223 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Integrated Weed Management, Yield, Carrot.
Abstract: Field experiment was conducted during rabi, 2010-11 at vegetable Research Station, ARI, APHU, Hyderabad to find out effective integrated treatment for wed control in carrot. The experiment consisting of fourteen treatments and three replications was laid out in randomized block design. The results indicated that the pre-emergence application of metribuzin @ 0.3 ha-1 followed by hand weeding at 30 days after sowing recorded the least weed count and dry weight favouring the highest yield of carrot (21.72 t ha-1) An application of pendimethalin c.s. metribuzin and pendimethalin in combination with one hand weeding produced significantly and higher yield of carrot than the application of these herbicides alone.
Location: TE 12 New Biology Building
Literature cited 1: Balraj Singh, 1994. Weed control studies in tomato. Vegetable Science 21: 26-28. Channappagoudar, B.B., Biradar, N.R., Bharmagoudar and Koti, R.V. 2007. Crop weed competition and chemical control of weeds in potato. Karnataka Journal of Agricultural Sciences 20 (4): 715-718.
Literature cited 2: Denesh, G.R., Murthy, K.N.K., Murali, K., Shankaranarayana, V. and Muniyappa, T.V. 2005. Integrated weed management in potato (Solanum tuberosum L). Environment and Ecology 23: Spl-1, 102-104. Govindra Singh, Bhan VM and Tripathi SS 1984 Effect of herbicides alone and in combination with weeding on tomato and associated weeds. Indian Journal of Weed Science XVI: 262-266.

ID: 59836
Title: CHARACTERIZATION AND IDENTIFICATION OF THERMOTOLERANT, FERMENTATIVE YEASTS FROM INDIGENOUS ALCOHOL STARTERS OF FOUR ASIAN COUNTRIES.
Author: Win Aung, Yuka Watanabe and Fumio Hashinaga.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 211-220 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Indigenous alcohol starters, Thermotolerant fermentative Yeasts, Industrial use, Conventional and molecular techniques.
Abstract: Of 73 yeast strains isolated from indigenous alcohol starters of Nepal, Myanmar, Cambodia and Indonesia, 7 strains that grew and fermented at above 42 ? C using the selected yeast strains, and the ethanol productivities were measured after 48 h incubation. Two strains, MN 1-3 and CS2-3 were found to be higher ethanol productivity of 6.2 and 6.7 % (w/v), respectively. Thermotolerant, fermentative yeast strains were identified by conventional and molecular techniques. In the molecular technique, polymerase chain reaction (PCR) amplified 18s r DNA and internal transcribed spacer 1 (ITS 1) genes using specific primers were analyzed. As a result, the strains selected were identified as follows: NP3-2 and MN1-5, Issatchenkia orientalis; MN1-3 and CS2-3, Saccharomyces cerevisiae; CS1-1, Pichia fabianii; RA2-1, Clavispora lusitaniae and RA3-3, Candida glabrata.
Location: TE 12 New Biology Building
Literature cited 1: Altschul, S.F., Madden, T.L., Schaffer A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research. 25: 3389-3402. Aung, W., Watanabe, Y. and Hashinaga, F. 2012. Isolation and phylogenetic analysis of two thermotolerant, fermentative yeast strains from liquid tape ketan (Indonesian rice wine). Food Science and Technology Research. 18: 143-148.
Literature cited 2: Barnett, J.A., Payne, R.W. and Yarrow, D. 2000. Yeasts: Characteristics and Identification. Cambridge University Press. Chaijamrus, S. and Mouthung, B. 2011. Selection of Thai starter components for ethanol production utilizing malted rice from waste paddy. Songklanakarin Journal of Science and Technology. 33: 163-170.