ID: 59865
Title: OPTIMIZATION OF CULTURAL CONDITIONS FOR BIOHYDROGEN PRODUCTION BY A PHOTOSYNTHETIC BACTERIUM RHODOCYCLUS TENUIS KU 017.
Author: Srinivas Munjam and K.Jyothi Rani.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 381-388 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Rhodocyclus tenuis, Bio-hydrogen, Immobisization, Cultural conditions, Light/dark, Anaerobiosis /aerobiosis.
Abstract: Anoxygenic phototrophic bacteria as a part of their photosynthetic metabolism release hydrogen which can be employed as non-conventional and eco-friendly energy source. Rc tenuis KU 017, one such organism isolated from paper industry effluents, was found to produce significant amounts of hydrogen under varied conditions. In the present investigations an effort was made to optimize the conditions for the maximum production of hydrogen by this organism. Actively growing cells were found to be more efficient and sustained hydrogen production for longer period than resting cells. Resting cells preferred light anaerobic conditions for hydrogen production and required more lag period for hydrogen production. Growing cells produced more hydrogen during early phase of growth. The organism was able to utilize a wide variety of organic substances as carbon/electron donors. Sorbitol, formate, lactate and glucose were found to be good sources of carbon for hydrogen production. Growing cells preferred pH 7.0, whereas resting cells preferred pH 6.8 with 120h optimum incubation period for hydrogen production. Growing cells produced more hydrogen under aerobic dark than aerobic light conditions. On the other hand, under anaerobic conditions hydrogen production was more in illuminated conditions. Hydrogen production was optimum at temperature range 25-40 ? C. At 30 ? C growing cells produced maximum hydrogen in 120h, while resting cells preferred 96h duration.
Location: TE 12 New Biology Building
Literature cited 1: Biebel, H. and Pfenning, H. 1981. Isolation of members of the family Rhodospirillaceae In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG, editors. The prokaryotes. pp 267-73.
Datta, M., Nikki, G. and Shah V. 2000. Cynobacterial hydrogen production. World JMicrobial Biotechnol. 16: 8-9.
Literature cited 2: David Levin, B., Lawrence Pitt. And Murray Love. 2004. Biohydrogen production: Prospects and limitations to practical applications. Int J Hydrogen Energy. 29: 173-85.
Francou, N.anf Vignais, P.M. 1984. Hydrogen production by Rhodopseudomonas capsulate cells entrapped in carregeenan beads. Biotechnol Lett. 6: 639-44.
ID: 59864
Title: A STUDY ON PSEUDOMONAS AERUGINOSA BIOFILM FORMATION USING ATOMIC FORCE MICROSCOPY.
Author: Ashwin Kannan, Subramanian Ramalingam and Pennathur Gautam.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 379-380 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pseudomonas aeruginosa, Biofilm, Force distance curve, Adhesion force.
Abstract: Biofilms are bacterial cells in a matrix of extracellular polymeric material. They are formed at air-liquid or liquid-solid interface. Formation of biofilms is undesirable since it causes failure of implants and biocorrosion of pipelines. Hence studies on biofilms are important. In this study, the adhesion force of Pseudomonas aeruginosa biofilms were 6.9 ? 0.2 nN and 11.8 ? 0.7 nN respectively. The results indicate that the adhesiveness of biofilm increases with incubation time.
Location: TE 12 New Biology Building
Literature cited 1: Lyczak, J.B., Cannon, C. and Pier, G.B. 2000. Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. Microb Infect. 2: 1051-1060.
Maa, J, Liu, B.F., Xu, Q.Y. and Cui, F.Z. 2005. AFM study of hippocampal cells cultured on silicon wafers with nano-scale surface topograph. Colloids and Surfaces B: Biointerfaces. 44: 152-157.
Literature cited 2: Oh, Y.J. Lee, N.R. Jo, W., Jung, W.K. and Lim, J.S. 2009. Effects of substrates on biofilm formation observed by atomic force microscopy. Ultramicroscopy. 109: 874-880.
Parsek, M.R. and Singh, P.K. 2003. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 57: 677-701.
ID: 59863
Title: ANTIBACTERIAL ACTIVITY OF BOSWELIA OVALIFOLIOLATA BAL. AND HENRY AN ENDEMIC PLANT TO TIRUMALA HILLS OF EASTERN GHATS, INDIA.
Author: M.Rambabu, S.KM. Basha, P. Uma Maheswari and C.V. Narasimha Murthy
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 377-378 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Boswellia ovalifoliolata-Bark, Gum, Antimicrobial activity.
Abstract: Boswellia ovalifoliolata N.P. Balakr & A.N. Henry (Burseraceae), vernacularly known as Konda sambrani or adavi guggilam, is a deciduous medium sized tree endemic to Seshachalam hills. The antibacterial activity of the bark and gum extracts of Boswellia ovifoliata were studied against B. subtilis, S.aureus S. pneumonia, E.coli, P.aeruginosa and P.vulgaris. by agar well diffusion method. The results show that ethanol, methanol and chloroform extracts of both bark and gum showed antimicrobial activity against B.subtilis, S.aureus S.pneumonia, and P.aeruginosa and P.vulgaris.
Location: TE 12 New Biology Building
Literature cited 1: Gamble, J.S. 1957. Flora of the Presidency of Madras. Vol: 1-3 B.S.I. Calcutta.
Gibbs, R.D. 1974. Chemotaxonomy of Flowering Plants. Vol, 1-4, Mc Gill Queen ' s University Press, London.
Literature cited 2: Herborne, 1973. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. Chapmen and Hall, London.
Nagaraju, N. and Rao, K.N. 1990. A survey of plant crude drugs of Rayalaseema, Andhra Pradesh. Ind. J. Ethno. Pharmacology. 29: 137-158.
ID: 59862
Title: ANTIFUNGAL ACTIVITY OF PIMPINELLA TIRUPATENSIS, BAL. & SUBR: AN ENDEMIC PLANT TO TIRUMALA HILLS OF EASTERN GHATS, INDIA.
Author: P. Uma Maheswari, S.K.M. Basha, M.Rambabu and C.V. Narasimha Murthy.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 373-376 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pimpinella tirupatensis, Leaf, Tuber, Stem, Antifungal activity, Germacrene D.
Abstract: Pimpinella tirupatensis Bal & Subr. (Family Apiaceae; local name, Kondakothimera) is a rare and endemic medicinal plant and restricted to the Seshachalam hills of the Eastern Ghats, India (Balakrishnan and Subramanyam 1960, Nair and Sastry 1998.) The whole plant of P. tirupatensis is used to treat cough, stomach, liver problems, asthma, ulcer and tooth ache (Reddy and Reddy 1998, Madhava Chetty et. Al., 2008). This plant root extract is also used to treat skin disease (Bakshu and Venkata Raju 2002, Jeevan Ram et al., 2004). The aim of the study is to investigate the antifungal activity of alcohol extract of leaf, stem and tuber of Pimpinella tirupatensis Bal & Subr. The leaf, stem and tuberous roots of Pimpinella tirupatensis (Apiaceae) were subjected extraction and tested against four fungal pathogenic strains for antifungal activity. The minimum inhibitory concentration of active extracts against four strains A. niger, F. oxysporum, R. solani and S. rolfsi. Were determined. The active extracts contained significant amounts of alkaloids, flavonols, flavones and volatile oils. Two important photochemical compounds Germacrene D (4.35 %) & and a-Humulene (1.45.6%) are present in rich amount. Germacrene are a class of volatile organic hydrocarbons, specifically, sesquiterpenesy produced in certain plant species and they are known for their antimicrobial and insecticidal properties, though they also play role as insect pheromones. P-Cymen is a volatile oil which found to have antioxidant property.
Location: TE 12 New Biology Building
Literature cited 1: Bakshu, L.M.D. and Venkata Raju, R.R. 2002. Essential oil composition and antifungal activity of tuberous roots of Pimpinella tirupatensis Bal.& Subr., and endemic taxon from eastern Ghats, India. Flavor and Fragrance Journal. 17: 413-415.
Balakrishnan, N.P. and Subramanyanm, 1960. A new species of Pimpinella from Tirupati hills, South India. Bull Bot. Surv. India. 2: 427-428.
Literature cited 2: Jeevan Ram, A., Md. Bakshu, L. and Venkata Raju, R.R. 2004. In Vitro Antimicrobial Activity of Certain Medicinal Plants from Eastern Ghats, India, used for skin diseases. J Ethnopharmaco. 90: 353-357
Madhava Chetty, K., Siraj, K. and Tulasi Rao, K. 2008. Flowering plants of Chittor district, Andhra Pradesh, India, Student press. Pp. 360-368.
ID: 59861
Title: DEVELOPMENT OF A MOLECULAR METHOD FOR DETECTION OF MYCOTOXIN CONTAMINATION IN FOOD GRAINS.
Author: Manorama Kanuri, Anurag Chaturvedi, Sreedhar Mulinti, Ravicharan Ameda, Janakipriya Sannasi, Prathima Namburi, Sunil C. Kulkarni and Krishna M.R. Bhagavatula.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 361-372 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Aflatoxin, Ochratoxin, Deoxynivalenol, Fumonism contamination, PCR detection, Aspergillus parasiticus, Aspergillus ochraceus, Fusarium culmorum, Fusarium verticilloides.
Abstract: Mycotoxin producing gene fragments in fungal strains were amplified using polymerase chain reaction (PCR) and real time PCR (RT-PCR) along with a Bioanalyzer in an attempt to develop a rapid assay for mycotoxin detection. Forward and reverse primers were designed from original gene sequences (obtained from GENBANK) of the three genes, aflQ (ordA), Tril13-DON, and pks, which are key genes involved in the production of three mycotoxins, namely,aflatoxin (AF), deoxynivalenol (DON), and ochratoxin (OTA), using DNASTAR Lasergene 8.0 version software. Primers were selected to amplify a 166 base pair fragment of aflQ from DNA of Aspergillus parasiticus and Aspergillus flavus, a 200 bp fragment of ochratoxin producing polyketide synthase gene from Aspergillus ochraceus, a 250 bp fragment of deoxynivalenol (DON) producing Tri 13 gene from DNA of Fusarium culmorum and a 300 bp fragment of fumonism producing FUM1 gene from DNA of Fusarium verticillioides. The fragments obtained were resolved on a DNA 1000 Labchip in the Agilent 2100 Bioanalyzer for visualizing the amplified fragments. Amplification of the gene fragments was also achieved using RT-PCR with TAQMAN probes as well as SYBRGREEN, with cT values ranging from 19 to 30 for the four genes. Duplex PCR was successfully attempted to simultaneously detect two sets of genes in one PCR reaction, namely aflQ and Tri13-DON, and FUM1 and aflQ. Mycotoxigenic ability of the fungi contaminating selected food grains, namely, groundnut, maize and rice, was confirmed using both molecular detection method for gene amplification, as well as LC-MS method for mycotoxin detection in the contaminated grains.
Location: TE 12 New Biology Building
Literature cited 1: Bottalico, A. 1998. Fusarium diseases of cereals: species complex and related mycotoxin profiles in Europe. J. Plant Pathol. 80: 85-103.
Brown, D.W., McCormick, S.P., Alexander, N.J., Proctor, R.H. and Desjardins, A.E. 2001. A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and Fusarium graminearum. Fungal Genet Boil. 32: 121-133.
Literature cited 2: Chandler, E.A. Simpson, D.R., Thomsett, M.A. and Nicholson, P. 2003. Development of PCR assays to TRI7 and Tri 13 trichothecene biosynthetic genes and characterization of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis. Physiol Mol Plant P. 62: 355-367.
D ' Mello J.P.F., Placinta, C.M. and Macdonald, A.M.C. 1999. Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity.Anim Feed Sci Tech. 80: 183-205.
ID: 59860
Title: MECHANISM STUDIES OF BIOSORPTION OF PB USING ASPERGILLUS NIGER BIOMASS.
Author: Anjali Krishnan and Lolly Jain.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 355-360 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: SEM, EDX, Immobilization, Atomic Absorption Spectrophotometer.
Abstract: Fungal strain-Aspergillus niger was isolated from effluent of chemical and pharmaceutical industry using SDA agar. The strain was sequenced by PCR METHOD. Pretreatment of the biomass obtained was carried out and the dried powdered form was used for biosorption of heavy metals. This dried biomass was immobilized in Ca-alginate beads. Ability of Pb biosorption with immobilized Aspergillus niger biomass was premeditated in the present study. The concentration of Lead in the filtrate was then analyzed by Atomic Absorption Spectophotometer. The biosorption mechanism was studied using suitable techniques of SEM and EDX. The SEM analysis of both the normal biomass and the biosorbed one showed a significant change in the surface morphology, wherein the normal biomass surface showed normal pattern whereas the biosorbed mass showed sticking and shrinking leading to elongation. The EDX results enabled to detect the elements which played a key role in biosorption. Suitable conditions for Aspergillus niger to take up lead were studied by optimization. Following which biosorptive capacity too was calculated by quantifying the metal absorbed. The biosorption capacity was testes on a waste water sample obtained and was found to have an efficiency of 87%.
Location: TE 12 New Biology Building
Literature cited 1: Ahmad, S. Zafar and Ahmad, F. 2005 Heavy Metal Biosorption potential of Aspergillus and Rhizopus sp. Isolated from wastewater treated soil. Journal of Applied Sciences and Environmental Management. 9: 123-126.
Ahmad, I., Ansari, M. and Aqil, F. 2006 Biosorption of Ni, Cr and Cd by multi tolerant Aspergillus niger and Penicillium sp. Using single and multi-metal solution. Indian Journal of Experimental Biology. 44: 73-76.
Literature cited 2: Awasthi, M. and Rai, L. 2004. Adsorption of nickel, zinc and cadmium by immobilized green algae and cynobacteria: a comparative study. Annals of Microbiology. 54 (3): 257-267.
Awofolu, O.R, O.J.Okonkwo and R.V.D. Merwe. 2006. A new approach to chemical modification protocols of Aspergillus niger and sorption of lead ion by fungal species. Electronic Journal of Biotechnology. 9: 341-348.
ID: 59859
Title: IDENTIFICATION OF MOLECULAR MARKERS (RAPDS) FOR NEMATODE RESISTANCE IN CHILLIES.
Author: Khagandra Mani Meduri, S.Siva Rama Krishnan, K.Raghu Prakash and S.Naga Lakshmi
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 351-354 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: BSA (Bulk Segregant Analysis), RAPD markers, Oligonucleotide primers
Abstract: A survey of chilli grown areas of Andhra Pradesh was undertaken to identify the predominant nematode race. Chillies (Capsicum annuum L.) production is seriously affected by root knot nematode disease to which noresistant source available in the cultivated germplasm to identify the resistance and susceptible germplasm of chilli. 60 RAPD markers were analyzed by bulk segregant analysis of the 60 RAPD markers analyzed none gave any polymorphim b/w the bulks.
Location: TE 12 New Biology Building
Literature cited 1: Lawrence, G.J., Finnegan, E.J., Ayliffe, M.A. and Ellis, J.G. 1995. The gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N. Plant Cell. 7: 1195-1206.
Michelmore, R.W. and Paran, I. 1991 Genetic Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci, (USA). 88: 9828-9832.
Literature cited 2: Moscone, E.A., Dareny, M., Ebert, I., Greilhuber J., Ehrenbrofer, F. and Hunzik, AT. 2003. Analysis of nuclear DNA content in Capsicum by flow cytometry and fuelzen densitometry. Annals Bot. 92: 21-29.
Quarrie, A.A., Jancic, V.L., Kovacevic, D., Steed, A. and Pekic, S. 1999. Bulk segregant analysis with molecular markers and its use for improving drought resistance in maize. J. Expt. Bot. 50: 1299-1306.
ID: 59858
Title: MANAGEMENT OF HETERODERA AVENAE ON WHEAT USING VESICULAR ARBUSCULAR MYCORRHIZA WITH OIL CAKES.
Author: Ameetha Sharma and P.C. Trivedi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 347-350 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Wheat, Heterodera avenae, Vesicular arbuscular mycorrhiza, Organic amendments.
Abstract: In the present study, efficiency of different oil cakes like Sesame and Neem, individually and in combination with different species of VAM were evaluated in decreasing the disease frequency of Heterodera avenae and enhancing the growth parameters of Triticum aestivum, L. variety Wh-147. Their efficacious nature was highly effective in reducing the nematode population and significant improvement was observed in various plant parameters under study. Neem cake alone and in combination with VAM fungi proved better than sesame cake. Out of two species of VAM isolated from the local fields, Glomus fasciculatum proved to be more effective, however Glomus fasciculatum and Gigaspora gigantean, both greatly decreased nematode population along with enhancement in growth parameters. Overall assessment revealed that both VAM and oil cakes can be explored as an alternative means of managing plant parasitic nematodes.
Location: TE 12 New Biology Building
Literature cited 1: Arya, R. and Saxena, S.K. 2009. Efficacy of rhizosphere fungi against root-knot nematode Meloidogyne incognita and Rhizoctonia solani in tomato crop. Indian J Nematol. 39: 113-6.
Gangwar, M. and Dutt, D. 2009. Effect of Azorhizobium and VAM inoculation on fertilizer economy and yield of maize (Zea mays L) Journal of Research Punjab Agricultural University. 46: 55-59.
Literature cited 2: Gerdemann, J.W. and Nicolson, T.H. 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc. 46: 235-244.
Hussey, R.S. and Roncadori, R.W. 1982. Vesicular Arbuscular Mycorrhiza my limit nematode activity and improve pant growth. Plant Disease, 66: 9-14
ID: 59857
Title: ISOLATION AND CHARACTERIZATION OF LIPASE PRODUCING BACTERIA FROM A BIOFERTILIZER.
Author: Ayona Jayadev and S.S. Navami.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 343-346 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Lipase, Biofertilizer, Municipal Solid Waste (MSW), Lipolytic bacteria, Olive oil agar plate.
Abstract: Microorganisms play a key role in making the planet clean and aesthetic by degrading the waste materials on the earth surface. They produce a wide range of enzymes by which they degrade the organic matter present on the earth surface. For the degradation of each kind of organic matter they produce specific enzymes. Isolation of the enzymes produced by native microorganisms prove to be of great importance in various industrial processes. The present study is undertaken to enrich and enumerate bacteria from a waste generated biofertilizer and to screen lipase producing bacteria. The various lipolytic bacteria isolated were also compared to find the most efficient lipid degrader. Finally culture conditions for optimum production and activity of lipase from his isolate was looked for. There was 232 x 10-4 CFU of bacteria per mg of biofertilizer. A total of 18 bacterial colonies were isolated from the sample. Out of these, 17 cultures showed production of lipase showing a decomposition zone around the culture in olive oil agar plates. The activities of all the 17 cultures were analyzed by inoculating in liquid media supplemented with olive oil and the quantity of production of fatty acids. All the cultures were found to have comparable lipase production. One culture, (Culture no. 12) was found to have a slightly higher activity than the rest. Effect of incubation time, temperature and pH on enzyme activity was optimized for culture number 12. It was identified that the best activity was shown at 24 hours at 35 ? C and at a pH at around 7.
Location: TE 12 New Biology Building
Literature cited 1: ACS Specifications, 1993. 8th ed., 95, Reagentchemicals, Washington DC.
Chima Ngumah, Jude Ogbulie, Justina Orji and Ekpewerechi Amadi, 2013. Potential of organic waste for biogas and biofertilizer production in Nigeria http: //dx.doi.org/10.5755/j01.erem.63.1.2912.
Literature cited 2: Demain, A.L. and Sanchez, S. 2009. Microbial drug discovery: 80 years of progress. The Journal of Antibiotics. 62: 5-16.
Gupta, R., Rathi, P. and Gupta, N. 2003. Bradoo, S, Biotechnol. Appl. Biochem., 37: 63-71.
ID: 59856
Title: A STUDY OF CYANOBACTERIAL METABOLITE PRODUCTION DURING DIFFERENT INCUBATION PERIODS USING OSCILLATORIA AND SYNECHOCYSTIS SPP
Author: P.K.Chouhan, P.Das and M.Sharma.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 339-342 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Cyanobacteria, Protein, Amino acids and incubation period.
Abstract: Protein and amino acids are nitrogenous compounds and are also used as precursor molecule in protein synthesis. A lot of cellular and membrane components are made up of protein; therefore organisms synthesized amino acids and protein during growing condition as primary metabolites. A cynobacterium is most diverse photosynthetic nitrogen fixing group and their thallus vary from unicellular to filamentous. They found in terrestrial habitat and also in water and fix atmospheric nitrogen in both micro-aerophilic and aerobic condition, in aerobic condition nitrogen fix by a specialized cell found only in filamentous forms called heterocyst. This fixed nitrogen assimilated in form of amino acids and then proteins. Protein, amino acids, vitamins and plant growth hormones are liberate by cyanobacteria in culture media; therefore these nitrogenous compounds improve overall soil fertility. Our study based on estimation of total protein and identification of amino acids in culture filtrate, cyanobacterial strains were isolated from Botanical garden soil of Govt. PG College, Mandsaur. This isolates were subjected to extracellular metabolite productions using BG-11 liquid medium with nitrogen, thereafter metabolites were estimated/ identified using standard protocols.
Location: TE 12 New Biology Building
Literature cited 1: Adam, M.S. 1999. The promotive effect of the cyanobacterium Nostoc muscorum on the growth of some crop plants. Microbiological Polinica. 48: 163-171.
Allen, M.M. 1968. Simple conditions for growth of unicellular blue-green algae on plates. J. Phycol. 4: 1-13.
Literature cited 2: Allen, M.M. 1973. Methods for cynophyceal.Stein, J.R. (ed). Handbook of phycological methods, culture methods and growth measurements, Cambridge Univ. press pp. 127-138.
Aref-Elham, M.M. 2001. Studies on Cynobacteria in Egypt. Ph. D. Thesis, Fac. Agric. Cairo Univ. Egypt.
ID: 59855
Title: SOLID WASTE MANAGEMENT USING VERMICOMPOSTING AND KODMICTM BIO-PEDESTAL COLUMN AND ITS UTILITY AS ORGANIC MANURE.
Author: M.G.Sumi, M. Vani, Dona V.Idicula and K.D. Mini.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 333-338 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Solid Waste, Biodegradable, Vermicomposting, Kodmic Bio-Pedestal Column, Organic Manure.
Abstract: Solid waste management is one of the biggest environmental problems faced by us today. Much of the residual waste materials of anthropogenic and animal origin, if not properly disposed, end up polluting terrestrial and aquatic systems. A large proportion of solid waste can be categorized as organic and biodegradable. Such degradable organic waste can be converted to useul and or/ environmental benign products using techniques such as microbial composting, vermicomposting, biogas plant, etc; there by. In the present study, we have used vermicomposting and KODMiC bio-pedestal column (KBPC; invented by Dr. Ignatious Konnikkara, Professor, Dept of Life Sciences, Calicut University, Kerala) two solid waste management methods to degrade house-hold waste. Solid waste was converted to organic manure using vermicomposting and KBPC method. Plant growth response, soil manured with vermicompost showed faster germination, flowering and fruiting time, while plants grown around KBPC unit were normal, healthier and had higher yield than the control plants. Increase in the microflora, i.e. the total number of bacterial and fungal colonies was observed in both methods, from the 0th-45th day. There was a increase in the NPK concentration compared to the control. Both methods had a major role in converting biodegradable waste into organic manure and prompted many plant growth characteristics. As a solid waste management option for a day to day biodegradable household wastes, KPBC method had several advantages over composting, as being a simple, cost effective, low maintenance, easy installation and did not need any expertise.
Location: TE 12 New Biology Building
Literature cited 1: Aneja K.R. 1996. Experiments in Microbiology, Plant Pathology and Biotechnology. K.R. Aneja, New Age International (P) Limited, New Delhi. 2nd ed.
Arancon, N.Q., Edwards, C.A., Atiyeh, R.M. and Metzger J.D. 2004. Effects of vermicomposts produced from food waste on green house peppers. Bioresource Technology.
Literature cited 2: Atiyeh, R.M., Edwards, C.A., Subler, S. and Metzger, J.D. 2000b. Earthworm-processed organic wastes as components of horticulture potting media for growing marigold vegetable seedlings. Compost Science and Utilization. 8 (3): 215-223.
Atiyeh, R.M., Lee, S.S., Edwards, C.A., Arancon, N.Q. and derived from earthworm-processed organic wastes on plant growth. Bioresource Technology. 84: 7-14.
ID: 59854
Title: GC-MS ANALYSIS OF PIMPINELLA TIRUPATIENSIS, BAL & SUBR. TUBER EXTRACT: AN ENDEMIC PLANT TO TIRUMALA HILLS OF EASTERN GHATS, INDIA.
Author: P.Uma Maheshwari, S.K.M. Basha, M.Rambabu and C.V.Narasimha Murthy.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 329-332 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: GC-MC Analysis, Pimpinella tirupatiensis-tuber chemical composition
Abstract: Pimpinella tirupatiensis Bal & Subr. (Family Apiaceae; local name, kondakothimera) is a rare and endemic medicinal plant and restricted to the Seshachalam hills of the Eastern Ghats, India (Balakrishnan and Subramanyam 1960, Nair and Sastry 1998). The whole plant of P .tirupatiensis is used to treat cough, stomach, liver problems, asthma, ulcer and tooth ache (Reddy and Reddy, 1998, Madhava Chetty et al., 2008). This plant root extract is also used to treat skin disease and also as antimicrobial agent (Jeevan Ram et al. 2004, Bakshu and Venkata Raju 2004). The aim of the study is to investigate the phytochemical analysis of alcoholic tuber extract of Pimpinella tirupatiensis Bal. & Subr by qualitative and GC-MS methods. The qualitative studies of the tuber revealed the presence of Tannins, Steroids, Alkaloids, Glycosides, Flavonoids and Phenolic compounds. In the GC-MS analysis, 24 bioactive phytochemical compounds are identified in the alcoholic extract. Among them two important photochemical compounds Germacrene D (4.35 %) & and a-Humulene (1.45.6 %) are present in rich amount. Germacrene are a class of volatile organic hydrocarbons, specifically, sesquiterpenesy produced in certain plant species and they are known for their antimicrobial and insecticidal properties, though they also play a role as insect pheromones. P-Cymen is a volatile oil which found to have antioxidant property.
Location: TE 12 New Biology Building
Literature cited 1: Adams, R. 1995. Identification of essential oil components by Gas Chromatography/Mass Spectroscopy. Allured Publishing Co., Carol Strea, IL. Delazar, A., E. Nazifi, A. Movefeghi, L. Nahar, H. Nazemiyeh, S.B. Moghadem, S. Asnaashari and Sarkar, S.D. 2009. GC-MS analysis of ornithogalum Procerum. Daru. 17: 33-36.
Bakshu, L.M.D., Venkata Raju, R.R. 2002. Essential oil composition and antimicrobial activity of tuberous roots of Pimpinella tirupatiensis Bal. and Subr., an endemic taxon from eastern ghats, India. Flavour and Fragrance Journal. 17: 413-415.
Literature cited 2: Balakrishnan, N.P. and Subramanyam, K. 1960. Bull. Bot. Surv. India. 427-428.
Harborne, J.B. 1973. Phytochemical Methods: Guide to Modern Techniques of Plant Analysis. Chapman and Hall, London.
ID: 59853
Title: EXPLORATION OF INDUSTRIALLY IMPORTANT ENZYME PRODUCING MICROORGANISMS FROM EXTRTEME FROM HABITATS OF DIBANG VALLEY, ARUNACHAL PRADESH, INDIA.
Author: Kodaparthi Ashwitha, G.Balakrishna, Pavan Kumar Pindi and A.Swaroopa Rani.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 321-327 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Extreme habitats, extremophiles, Cellulases, Lipases, Proteases and CFU.
Abstract: Microbial diversity constitutes the most extraordinary reservoir of life in the biosphere that we have only just begun to explore and understand. A preliminary investigation on the microbial diversity of potential extracellular enzyme producers was done by direct screening methods to provide an insight on the possibility to use different moderate extremophiles as a source of extremophilic enzymes in biotechnological processes. Samples were collected randomly from soil sediments of Dibang valley, Arunachal Pradesh, India and their physico-chemical characteristics such as pH, moisture content and Nitrogen, Phosphate and Potassium content were determined. The dynamics of microbial population was investigated by using serial dilution method on different microbiological media to ensure maximum microbial diversity. The isolated bacteria, actinomycetes and fungi were screened for their cellulose, protease and lipase activities. The study revealed that bacterial isolates showing higher zone of cellulolytic activity belong to Bacillus, Pseudomonas and Serrartia sp, while fungal isolates mostly belong to Aspergillus and Penicillium sp. The study has suggested that the potential enzyme producing isolates were identified indicating that the extreme habitat soils are rich source of industrially important enzyme producers.
Location: TE 12 New Biology Building
Literature cited 1: Adinarayana, K.P., Ellaih, D.S. Prasad. 2003. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS. Pharm. Sci. Tech. 56 (4): 1-9.
Ajwa, H.A., Dell, C.J. and Rice, C.W. 1999. Changes in enzyme activities and microbial biomass of tall grass prairie soil as related to burning and nitrogen fertilization. Soil Biol. Biochem. 31: 769-777.
Literature cited 2: AOAC 1990. Official Methods of Analysis. 15th ED. AOAC, U.S.A.
Arpigny, L.L and Jaeger, K.E. 1999. Bacterial lipolytic enzymes: classification and properties. Biochemistry Journal 343: 177-183.
ID: 59852
Title: DECOLOURIZATION OF TEXTILE AZO DYES BY LACCASE PRODUCING BACILLUS SPP.ISOLATED FROM SOIL.
Author: Viraj Krishna Mishra and Himani Sharma.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 313-319 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Azo dyes, Spore Laccase, Bacillus Spp, Guiacol, Dye decolorization.
Abstract: Twenty five bacterial isolates exhibiting laccase screened on the basis of 0.1 % guaiacol utilization from field soil. LB media containing Cu2+ was used for isolation screening. Out of twenty five only three isolates were selected on basis of dye decolorization activity and identified as Bacillus spp. on basis of biochemical characterization. Selected isolates D1023, D1032 and D1033 were transferred to 2xSG medium to increase and fasten spore production. Selected isolates could grow from temperatures ranging from 30 ? C to 55 ? C and showed optimum growth temperature and pH at 37 ? C and 7.0, respectively. Maximum growth was observed using fructose as carbon source and at 2.6 % concentration of nutrient broth. Spore laccase of isolates D1023, D1032, D1033 exhibited the excellent potential to decolorize different azo dyes: Amido Black, Orange G, Methyl Orange, Congo Red and Solochrome Black at a concentration of 100 mg/L maximum up to 83. 43 %, 91.42 %, 78. 14 %, 72.42 % and 71.31 % respectively during a time period of 4 days at 37 ? C (pH 7) during static conditions, which suggests the potential application of spore laccase in dyestuff treatment.
Location: TE 12 New Biology Building
Literature cited 1: Asad, S., Amoozegar, M.A., Pourbabaee, A. A., Sarbolouki, M.N. and Dastgheib, S.M.M. 2007.Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresource Technogyl 98: 2082-2088.
Bras, R., Gomes, A., Ferra, M.I.A., Pinheiro, H.M, Gonc and alves I.C. 2005. Monoazo and diazo dye decolorisation studies in a methanogenic UASB reactor. J. Biotechnol. 115: 57-66
Literature cited 2: Banat, I.M., Nigam, P., Singh, D. and Marchant, R. 1996. Microbial decolorization of textile-dye containing effluents: a review. Bioresource Technology. 58: 217-227.
Chung, K.T. and Cerniglia, C.E. 1992. Mutagenicity of azo dyes: structure activity relationship. Mutation Research. 277: 201-220.
ID: 59851
Title: PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL ANALYSIS OF SOLANUM MURICATAM FRUIT EXTRACTS.
Author: A. Nirmala, G.Manimekalai, P. Vasanthi, A. Jagajothi and V.K Evanjelene.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 307-311 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Solanum muricatam, Antimicrobial, Phytochemicals, Medicinal plant.
Abstract: Medicinal plants are a valuable natural resource and regarded as potentially safe drugs. The main objective of the present research work was to see antidiabetic activity of Solanum muricatam fruit extracts obtained by different (Hexane, Ethyl alcohol and Hydro alcohol) extracts. Phytochemical screening showed positive results for alkaloids, flavonoids, steroids, terpenoids, phenols, tannin, carbohydrates, oil and resins in fruit extracts. The extracts showed rich phytochemical activity. Antimicrobial activity was evaluated against different bacterial strains (both gram positive and gram negative). It was observed that hydro alcohol extract of Solanum muricatam shows better activity in S. typhi ' A ' (40 mg/well) was the most potent extract which showed significant inhibition of the growth of bacterial strains.
Location: TE 12 New Biology Building
Literature cited 1: Bauer R.W., Kirby M.D.K., Sherris J.C. and Turck M. 1966. Antidiabetic susceptibility testing by standard sigle disc diffusion method. American Journal of Clinical Pathology.45.
Bauer, R.W. and Kirby, M.D.K., Sheris, J.C. Turck, M. 1966. Antibiotic susceptibility testing by standard single disc diffusion method. American Journal of Clinical Pathology. 45: 493-496.
Literature cited 2: Chesson, A., C.S. Stewart and R.J. Wallace, 1982. Influence of plant phenolic acids on growth and cellulytic activity of rumen bacteria. Applied envir.Microbiol., 44: 597-603.
Chioma, A., Anosike, Onyechi Obidoa and Lawrence, U.S. Ezeanyika 2012. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum) DARU Journal of Pharmaceutical Sciences. 20: 76.