Enter records

ID: 59880
Title: STUDIES ON THE PATHOGENIC POTNTIAL OF RACES IN SPHAEROTHECA FULIGINEA INFECTING CUCURBITS IN EASTERN UTTAR PRADESH, (INDIA).
Author: Sanjay Gangwar and Kiran Mishra.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 465-468 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Powdery mildew, Races, Cucurbits, Host differential.
Abstract: Pathogenic potential of races in Sphaerotheca fuliginea on cucurbits were analysed in Eastern part of Uttar Pradesh (India). The study showed the occurrence of three races in S. fuliginea in this area. Race-3 was most wide spread and infected most of the cultivated cucurbits. Race-2 was restricted to green melon, Citrullus vulgaris var. fistulosus and Race-1 to sponge gourd, Luffa cylindrical. In addition Race-1 infected a few other cucurbits. L. cylendrica, C. vulgaris var. fistulous and C. sativus are proposed as differential hosts for identification of the three races in S. fuliginea isolates from cucurbits.
Location: TE 12 New Biology Building
Literature cited 1: Ballantyne, B.J. 1975. Powdery mildew of cucurbitaceae: Identity, distribution, host range and sources of resistance. Proc. Linn. Soc. New South Wales. 99: 100-120. Cohen, R., Burger, Y. and Katzir, N. 2004. Monitoring o physiological races of Podosphaera xanthii (Syn.Sphaerotheca fuliginea), the causal agent of powdery mildew in cucurbits, factors affecting race identification and the importance for research and commerce. Phyto-Parasitica. 32: 174-183.
Literature cited 2: Hosoya, K., Narisawa, K., Pitrat, N. and Ezura, H.1999. Race identification in powdey mildew (Sphaerothera fuliginea on melon (Cucurmis melo L.) in Japan. Plant Breeding. 188: 259-262. Kaur, J. and Jhooty, J.S. 1986. Presence of Race-3 of Sphaerotheca fuliginea on muskmelon in Panjab. Indian Phytopath. 39: 297-299.

ID: 59879
Title: PHYSICAL, CHEMICAL AND MICROBIOLOGICAL CHARACTERISTICS OF A DUMP SITE NEAR THE UNIVERSITY OF PORT HARCOURT, PORT HARCOURT IN NIGER DELTA AREA OF NIGERIA.
Author: H.O. Stanley, I.F. Onianwah and P.O. Okerentugba.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 459-464 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Physicochemical, Microbiological characteristics, Dump Site, University of Port Harcourt, Niger Delta, Nigeria.
Abstract: The study was carried on the dump site at the University of Port Harcourt main gate and the characterization was done on microbial and physicochemical properties of the dump. The result of the analysis revealed that the site has pH range of 8.0 to 9.5 and a temperature range of 36 ? C to 42 ? C with a mean annual rainfall of 677.12 mm. The conductivity of the refuse samples (5.5 to 190.5 uscm1) was high due to the presence of ions. The concentration of Cadmium and Lead (1.74 to 2.91 mg/kg and 3.91 to 5.82 mg/kg respectively) were high compared to (0.36 to 0.89 mg/kg) Mercury and this may be attributed to the volatile nature of Mercury. The high values of Biochemical Oxygen Demand and Chemical Oxygen Demand is responsible for the high level of aerobic activities at the site. Further chemical analysis revealed the presence of Hydrocarbon (2.87 to 4.26 mg/kg). The total heterotrophic bacteria count ranged from 2.9 x107 to 9.2 x 107 cfu/ml and 3.0x 109 cfu/g; while total fungal count is 2.7 x 103 to 5.6 x 106 cfu/ml and 1.4 x 105 to 2.6 x 105 cfu/g. Aerobic microorganisms such as Shigella, Samonella, Pseudomonas, Bacillus, Micrococcus, Staphylococcus, Escherichia, Torulopsis, Candida, Saccharomyces, Rhizopus, Aspergillus, Fusarium and Mucor were isolated and characterized from the samples. The presence of heavy metals and pathogenic microorganisms poses danger to public health and as such, requires checks.
Location: TE 12 New Biology Building
Literature cited 1: Allen, A.R. 2001. Containment Landfill: The Myth of Sustainability. J. Eng. Geology. 603-19. Alloway, B.J. 1990. Cadmium. In B.J. Alloway (Ed). Heavy metals in soil. John Wiley and sons.New York. 100- 124.
Literature cited 2: Attahi, F. 1999. Abidjan, Cote d Ivoire. In Managing the Monster. Urban waste and governance in Africa, (ed. A.G. Onibokun) 11-48, IDRC, Ottawa. Aust, A.S., Lamar, R. and Arisoy, M. 1998. Degrading Environmentally persistent organic pollutant compounds. Environmental Contamination and Toxicology. 60: 872-876.

ID: 59878
Title: GROWTH OF Bacillus Megaterium CSK2, Bacillus Subtilis CSK3 AND Bacillus Subtilis CSK4 ISOLATED FROM COAL MIXED SOIL IN DIBENZOTHIOPHENE-CONTAINING MEDIUM.
Author: Megga Ratnasari Pikoli, Pingkan Aditiawati, Akhmaloka, Dea Indriani Astuti and Reni Wijayanti.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 453-460 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Bacillus megateium, Bacillus subtilis, Dibenzothiophene, Desulfurization, Coal.
Abstract: Biodesulfurization is an environmentally friendly technology to reduce organic sulfur in coal by utilizing bacterial metabolism. Isolation and identification of bacteria from coal-mixed soil from South Sumatra has been conducted in order to obtain bacteria which desulfurize organic sulfur in local coal. Three viable isolates, i.e CSK2, CSK3, and CSK4, were obtained by using a mineral medium containing glucose as carbon source and dibenzothiophene as sole sulfur source. Molecular identification of 16 SrRNA gene sequences showed that the isolates had 99% similarity to genus Bacillus, which CSK2 was related to B. megaterium, while the isolates CSK3 and CSK4 were related to B. subtilis. Growth examination of the isolates within 48 hours in the dibenzothiophene in the period of incubation was 37.4, 16.7, and 25.9 percent of the initial 0.1 mM dibenzothiophene, by B. megaterium CSK2, B. subtilis CSK3, and B. subtilis CSK4 respectively. The results showed that the isolates derived from coal-mixed soil have potential to be developed in desulfurization of organic sulfur in coal.
Location: TE 12 New Biology Building
Literature cited 1: British Petroleum. 2013. BP Statistical Review of World Energy June 2013. http: // www.bp.com/content/dam/bp/pdf/statistical -review/statistical_review_of _world_energy_2013.pdf. Constanti, M., Giralt, J., and Bordons, A. 1994. Desulphurization of dibenzothiophene by bacteria. World Journal of Microbiology and Biotechnology. 10 (5): 510-516.
Literature cited 2: Etemadifar, Z., Emtiazi, G., and Christofi, N. 2008. Enhanced desulfurization activity in protoplast transformed Rhodococcuserythropolis.Am.Eur.J.Agric.Environ.Sci 3: 795-801. Gunam, I.B.W . Yaku, Y., Hirano, M., Yamamura, K., Tomita, F., Sone, T. and Asano, K. 2006. Biodesulfurization of alkylated forms of dibenzothiophene and benzothiophene by Sphingomonassubarctica T7b. Journal of Bioscience and Bioengineering. 101 (4): 322-327.

ID: 59877
Title: EARTHWORM MEDIATED BIOREMEDIATION OF PHENOL
Author: Neethu Kamarudheen, Christy George, Ishani Bose, Mythili Sathiavelu and A.Sathiavelu.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 447-451 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Earthworm, Vermicomposting, Phenol, Bioremediation, 4-amino antipyrine.
Abstract: Our study dealt with the phenol bioremediation potential of Earthworms which were collected from a vermicomposting unit in Vellore, Tamil Nadu and maintained under proper growth conditions. Different concentrations of phenol ranging from 10 ppm to 50 ppm wee taken for the assays. All the bioremediation assays were done in the soil with phenol dispersed in water and remediation rates were observed for 24 hours, 48 hours, 72 hours, 96 hours and 120 hours. The initial concentration of phenol was increased eventually and the rate of bioremediation was estimated using 4-amino anti pyrine method. The phenol degradation activity of the gut microflora of the earthworm along with the soil microflora was also observed on a formulated media. The amount of phenol bioremediated was found higher in case of earthworm and not the microflora solely. It was found to be 30 ppm in 48 hours when the initial concentration was 50 ppm.
Location: TE 12 New Biology Building
Literature cited 1: Arun, K., Ashok, M. and Rajesh, S. 2011. Crude oil PAH constitution, degradation pathway and associated bioremediation microflora, International Journal of Environmental Sciences. 1 (7): 1420-1439. Chakrabarty, A.M. and Daubaras, D. 1992. The environment, microbes and bioremediation: microbial activities modulated by the environment. Biodegradation. 3: 125-135.
Literature cited 2: Dipty Singh and Fulekar, M.H. 2007. Bioremediation of phenol using microbial consortium in bioreactor, Innovative Romanian Food Biotechnology. 1: 31-36 Eijsackers, H., Van Gestel, C.A.M., De Jonge S., Muijs B., and Slijkerman D. 2001. Polycyclic Aromatic Hydrocarbon-Polluted Degraded Peat Sediments and Earthworms: A Mutual Interference. Ecotoxicology 10: 35-50.

ID: 59876
Title: MOLECULAR DOCKING STUDIES ON TRANSPOSASE PROTEIN OF STREPTOCOCCUS PNEUMONIAE-TCH8431/19A STRAIN.
Author: Balasankar Karavadi and M. Xavier Suresh
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 443-446 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pneumonia, Streptococcus pneumonia, TCH8431/19A, Homology, Docking.
Abstract: Streptococcus pneumonia causes mucosal diseases in human upper respiratory tract. TCH8431/19A strain of Streptococcus pneumonia is extremely virulent in pneumococcal pathogenesis which contains Protein coding gene (HMPREF0837_11199) which synthesizes transposase protein. In this article we performed the homology modeling studies of the virulent protein which is synthesized by the protein coding gene and validated he nature of the receptor as a future drug target for TCH8431/19 A Strain of streptococcus pneumonia. We have also identified specific ligands for the above mentioned protein by using the virtual structure based ligand screening approach. Protein-ligand complexes have been analyzed by docking studies using Discovery Studio and interactions have also been visualized along with the validation of pharmacokinetic descriptors.
Location: TE 12 New Biology Building
Literature cited 1: Avery, O.T., MacLeod, C.M., McCarty, M. 1944. Studies on the chemical nature of the substance inducing transformation of pneumococcal types. J. Exp. Med.79: 137-158 Chou, J., Lemaux, P.G., Casadaban, M., Cohen, S.N. 1979. Transposition protein of Tn3: identification and characterization of an essential repressor controlled gene product. Nature. 282: 801-806
Literature cited 2: Joe Dundas et al. 2006. CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues. Nucl.Acids Res. 34: W116-W118. Kleckner, N. 1990. Regulation of transposition in bacteria. Annu. Rev. Cell Biol. 6: 297-327.

ID: 59875
Title: A STUDY ON THE LIME-CEMENT STABILIZED MARINE CLAY.
Author: D.Venkateswarlu, M. Anjan Kumar, G.V.R. Prasad Raju and D.S.V. Prasad.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 437-442 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Cement, Marine clay, Lime.
Abstract: Comprising over 70 % of earth ' s surface, water and its adjacent areas is undoubtedly the most precious natural resources that exist on our planet. Areas surrounded by the coast have been highly utilized since ancient times. Several National and International authorities have mandated to develop coastal structures as part of infrastructure development. The various potential problems associated with these Marine soils include land slippage, slope instability, poor foundation support and poor drainage. Problems often arise when roads or other structures are to be constructed on soft ground. In the case of Flexible Pavements, unless the subgrade is appropriately treated at the construction stage, the transportation cost will increase substantially due to deteriorated pavement performance. There are many methods of stabilization of subgrade marine clay. Treating the soil with lime, cement and lime-cement combination is a unique technique to improve the engineering properties of the marine clay. The main objective of this work is to study the effectiveness of additives at different percentages on the engineering properties of marine clay.
Location: TE 12 New Biology Building
Literature cited 1: Bansal, R.K., Pandey, P.K. and Singh, S.K. 1996. Improvement of typical clay for roads subgrades with hydrated lime. Proceedings of National conference on Problematic Subsoil Conditions, Terzghi-96, Kakinada, India, 1996, pp. 193-197. Basack, S. and Purkayastha, R.D.R.D. 2009. Engineering properties of marine clays from the eastern coast of India. Journal of Engineering and Technology Research. 1 (6): 109.114.
Literature cited 2: Kamon, M. and Nontananandh, S. 1991. Combining industrial wastes with lime for soil stabilization. Journal of Geotechnical Engineering.17 (1): 1-17. Katsutada Onitsuka, Chirdchamin Modmoltin and Masakazu KOUNO 2001. Investigation on Microstructure and Strength of Lime and Cement Stabilized Ariake Clay. Reports of the faculty of science and engineering saga University 30 (1).

ID: 59874
Title: MARKER ANALYSIS AND FIELD SCREENING STUDIES IN RICE FOR BLAST RESISTANCE.
Author: A.Chandrasekar, N.Kumaradivel and R. Rabindran.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 433-436 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Rice, blast, Disease, Marker.
Abstract: Rice improvement for durable resistance to blast is difficult due to its complexity. Rice blast, caused by fungus Magnaporthegrisea, is a serious disease causing considerable economic damage worldwide. Blast is one of the destructive diseases, which causes maximum yield losses in rice. Best way to overcome the disease is to breed for disease resistant cultivars/parental lines of hybrids. The present study was conducted to analyse blast resistance in rice. Rice genotypes viz., VRPI, VRP2, B95-1 X Tetep, BPT5204 X Tetep, CO39 and CO43 were screened for blast resistance in artificial condition. The study indicated that the genes Pi9 and Pi54 recorded resistance reaction to blast pathogen occurring in Tamil Nadu. The blast resistance gene, Pi9 is present in VRP1 and VRP2 and another blast resistant gene Pi54-MAS were already identified for the genes Pi9 and Pi54 respectively. The present study indicated that the tightly linked markers showed polymorphism between resistance and susceptible rice genotypes indicating the possibility of initiating MAS for blast resistance in rice.
Location: TE 12 New Biology Building
Literature cited 1: Ashkani, S., Raffi, M.Y., Rusli, I., M. Sariah, Abdullah, S.N. A., Rahim, H.A. and Latif, M.A. 2012. SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryzasativa L) Plant Mol. Biol. Rep. 30: 79-86. Bonman, J.M. Khush, G.S. and Nelson, R.J. 1992. Breeding rice for resistance to pests. Annu Rev Phytopathol 30: 507-528.
Literature cited 2: Chen, D.H. Zeigler, R.S., Ahn, S. and Nelson, W. 1996. Phenotypic characterization of the rice blast resistance gene Pi2 (t). Plant Dis 80: 52-56. Filippi M.C and Prabhu, A.S. 2001. Phenotypic virulence analysis of Pyriculariagrisea isolates from Brazilian upland rice cultivars. Pesquisa Agropecuaria Brasileira 36: 27-35.

ID: 59873
Title: ARTIFICIAL SCREENING FOR BACTERIAL BLIGHT RESISTANCE AND VALIDATION OF MARKERS FOR MARKER-ASSISTED SELECTION.
Author: A.Chandrasekar, N.Kumaravadivel and R.Rabindran.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 429-432 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Rice, Bacterial leaf blight, Disease, Marker.
Abstract: Rice (Oryzasativa L.) is the most important food crop of the world. Breeding for resistance against Bacterial blight (BB), a destructive disease in rice, is a major challenge because of the rich diversity of the pathogen, Xanthomonasoryzaepv.oryzae. Rice genotypes viz., B95-1 X Abhaya (xa13), B95-1 XTetep (Xa21), B95-1 XKavya (Xa 21), B95-1 X Aganni (Xa21), ADT38 and CO43 were screened for bacterial blight resistance in artificial condition. The study indicated that the genes xa13and Xa21 recorded resistance reaction to bacterial blight pathogen occurring in Tamil Nadu. Tightly linked markers xa13-prom and Pta248 are available for the genes xa13 and Xa21 respectively.
Location: TE 12 New Biology Building
Literature cited 1: IRRI-Standard evaluation system.1996. International Rice Research Institute, Manila, Philippines. Jung-Pil, S. Jeung, J.U. Hwan Noh, T.Cho, Y.C. Park, S.H. Park, H.S.Shin, M.S. Kim, C.K. and Jena, K.K. 2013.
Literature cited 2: Kauffman, H.E., Reddy, A.P.K., Hsien, S.P.Y. and Merca, S.D. 1973. An improved technique for evaluating resistance of rice varieties to Xanthomonasoryzae. Plant Dis Rep 57: 537-541. Khush, G.S. and Virmani, S.S. 1985. Breeding rice for disease resistance. In Russell, G.E. (ed), Progress in Plant Breeding, Vol.I, Butterworths, London, 239-279.

ID: 59872
Title: REMOVAL OF IRON FROM GROUND WATER USING EMBLICA OFFICINALIS BASED WOOD CHARCOAL.
Author: T.J. Thomas and G.Madhu
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 423-427 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Emblica officinalis, Low cost adsorbent, Iron, Adsorption, Isotherms.
Abstract: The presence of iron was observed in few ground water samples analysed from the tube wells of Wayanad district. It is observed that these tubewells are more than 400 feet in depth. The presence of iron in ground water of tubewells that are 400 feet or above in depth are highly possible. The removal of iron from groundwater by a low cost natural adsorbent was investigated in the present study. The wood charcoal prepared from the hard wood of Emblica officinalis was investigated for the effective removal of iron from water samples. At first the study was conducted with synthetic solution and the efficiency of the adsorbent was confirmed and then it was directly used for the removal of iron from the ground water samples. The influence of pH, contact time, adsorbent dosage and initial concentration on adsorption efficiency was analysed. The adsorption efficiency was found to be influenced by pH, adsorbent dosage, contact time and initial concentration of the metal ion. The most favourable pH was found to be 4 and the removal of the metal gave the best result with a minimum contact time of 60 minutes. The optimum adsorbent dosage was found to be 800-900 mg. The initial concentration of the solution also was found to be influence the % removal of iron from the water samples. The equilibrium adsorption capacity of the adsorbents used for iron was measured and extrapolated using Freundich and Langmuir isotherms. The IR spectrum of the adsorbent was recorded to analysis the presence of functional groups influencing the metal ion adsorption on the plant based adsorbent.
Location: TE 12 New Biology Building
Literature cited 1: Anusha, G. 2011. Removal of iron from waste using bael fruit shells as adsorbent. 2011 2nd International Conference on Environmental Science and Technology, IPCBEE.6, IACSIT Press Singapore. Deans, J.R. and Dixon, B.G. 1992. Uptake of Pb2+ and Cu2+ by novel biopolymers. Water Res. 26 (4): 469-472.
Literature cited 2: Hanzlik, P., Jehlicka, J., Weishauptova, Z., and Sebek, O. 2004. Adsorption of copper, cadmium and silver from aqueous solutions unto natural carbonaceous materials. Plant Soil Environ. 50 (6): 257-264. Rafika Souag, Djilai Touaibia, Benchreit Benayada and Ali Boucenna. 2009. Adsorption of heavy metals (Cd, Zn and Pb) from water using Keratin powder prepared from Algerian Sheep Hoofs. European J. of Scientific Research. 35 (3): 416-425.

ID: 59871
Title: MYCOBIOTA OF DECOMPOSING FLORAL WASTE MATERIALS.
Author: Shobha Shouche, Praveesh Bhatt, Mrs.Zeemi Nema and Sudhir Kumar Jain.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 417-422 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Floral waste, Vermicoposting, Filamentous fungi, Isolation, Identification.
Abstract: The role of fungi in decomposition of organic compounds is well known. Fungal diversity reflects the composition of mycobiota related to waste materials. The present study has concerned about the isolation & identification of fungi related to floral wastes. The process was done by conventional method of vermicomposting with different proportions of floral wastes and cattle dung, using appropriate media and techniques. The collected samples were examined by plate count analysis on CDA medium revealed average number of fungi from all the three seasons was 1.37x105 CFU/ml. In rainy season average factor was 1.065 where as in winter and summer season average factor was 0.978 and 0.958 respectively. Among all the fungi 12 strains were obtained more abundantly in all the three seasons .Aspergillus species were obtained as the most dominant genus among fungal community. Results were shown to presence of diverse genera of fungi included Mucor, Cladosporium, Alternaria, Aspergillus and Penicillium were dominant genera. Aspergillus and Alternaria were found to be most frequent isolated genera.
Location: TE 12 New Biology Building
Literature cited 1: Anastasi, A., Varese, G.C., and Marchisio, V.F. 2005. Isolation and identification of fungal communities in compost and vericompost. Mycologia. 97 (1): 33-44. Ashraf, R. and Ali, T.A. 2006. Effect of oil (crude petroleum) on the survival and growth of soil fungi. Int. J. Biol. Biotech 3 (1): 127-133.
Literature cited 2: Dubey, R.C. and Maheshwan, D.K.2005. A text of Microbiology. Multicolour illustrative ed. S. Chand and Company Ltd. Ram Nagar, New Delhi 110055. Edward, C.A. and Fletcher, K.E. 1988. Interactions between earthworms and microorganisms in organic matter breakdown. Agriculture Ecosystem and Environment. 24: 135-147.

ID: 59870
Title: ANTIMICROBIAL ACTIVITY OF SOME ETHNO-MEDICINAL PANTS USED BY TRIBES OF DISTRICT GUNA, MADHYA PRADESH, INDIA.
Author: Rakesh Samar, Manju Jain and P.N.Shrivastava.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 411-416 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Ethno-medicinal plants, Tribes, Antimicrobial activity.
Abstract: The present study was undertaken to investigate the antimicrobial activity of some ethno-medicinal plants used by tribes of district Guna, Madhya Pradesh, India. Antimicrobial activity of 04 ethno-medicinal plant extracts were evaluated against three bacterial strains. The collected ethno-medicinal plants were used in folk medicine in the treatment of jaundice, laxatives, opthalmics, anthelmics, expectorants, purgative and self-medication. All the tested plants, Acacia Arabica, butea monosperma, Cassia fistula and Cassia tora exhibited antimicrobial activity against one or more of the tested microorganisms, Staphylococcus aureus, Salmonella typhimurium and Streptococcus pyogenes at five different concentrations of 20, 40, 60, 80, and 100 mg/disc. Among the tested plant extracts, ethanolic extracts exhibited a higher degree of antimicrobial activity as compared distilled water extracts. The ethanolic extracts of Acacia Arabica and Cassia fistula were most active against Staphylococcus aureus and Salmonella thyphimurium.
Location: TE 12 New Biology Building
Literature cited 1: Abo, K.A., Laski S.W and Adeyemi, A.A. 1999. Laxative and antimicrobial properties of Cassia species growing in Ibadan. Nigerian Journal of Natural products and Medicine. 3: 47-50. Agrawal, M.K., Rathore, D., Goyal, S., Varma, A. and Varma, A. 2013. Antibacterial efficacy of Brassica campestris Root, Stem and Leaves extracts. Internaional Journal of Advanced Research. 1 (5): 131-135.
Literature cited 2: Agrawal, M.K., Goyal, S, Varma, A. and Varma, A. 2012. Antibacterial activities of some antibiotics against clinical isolates of certain bacterial strains with special reference to Lantana camara leaf extracts. Indian Journal of Scientific Research. 3 (2): 149-154. Agrawal, M.K., Varma, A.K., Dwevedi, S., and Saify, T. 2007. The antibacterial activity of the extracts of Lantana camara leaves against the skin disease causing bacteria. Indian Journal of Applied Life Sciences. 3(2): 15-16.

ID: 59869
Title: PRODUCTION AND PURIFICATION OF POLYPHENOLOXIDASE FROM ASH GUARD PEEL.
Author: Zoya Javed, Shailendra Kumar Shrivastva and Gyan Datta Tripathi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 405-409 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Polyphenol oxidase, thermophillic Bacillus subtalis, DEAE Cellulose and Ammonium sulphate precipitation.
Abstract: Polyphenol oxidases are enzymes that catalyze the oxidation of certain phenolic substrates to quinines in the presence of molecular oxygen. Polyphenol oxidases are widely used in several applications like food industry, medicine, wastewater treatment etc. In this study, detection, purification and characterization of polyphenol oxidase from thermophilic Bacillus subtilis, which was isolated from soil samples. Chemical analysis of Ash Gourd had been performed the chemical component, Protein 12.4 mg, Total soluble sugar 1.1mg, Moisture Content 93.1 % and Organic content 0.61 % was recorded. By using these values a media was standardized for the growth of bacteria which was significantly used for production of Polyphenol Oxidase enzymes. The enzyme was purified by ammonium sulphate precipitation followed by DEAE Cellulose ion exchange chromatography.
Location: TE 12 New Biology Building
Literature cited 1: Adnan, T.A.B.T., Augustin, M.A. and Ghazali, H.M. 1986. Polyphenoloxidase from Starfruit (Averrhoa carambola, L). Pertanika. 9 (2): 219-224. AOAC 1965. Official methods of analysis of the association of Official Agricultural Chemists, 10 th edn., Washington, D.C., Rodriguez Couto.S. and J.L. Toca Herrera. 2006. Industrial and biotechnological applications of laccases: a review: Biotechnol Adv 24 (5): 500-13
Literature cited 2: Biglelow, P. and Wyman, E. 2004. Production of cellulolytic on bagasse pretreated with chemicals. Applied Biochem. Biotechnol. 102: 78-82. Carey and Francis, A. 1996. Organic Chemistry. New York: McGraw Hill.

ID: 59868
Title: COMPARATIVE STUDY OF DNA EXTRACTION METHODS IN MARINE FISH SPECIES.
Author: N.Pratima, Anurag Chaturvedi, K.Manorama, M.R.Krishna Bhaghavathu and M.Sreedhar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 399-403 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Fish species, DNA extraction buffer, Fish DNA isolation kit.
Abstract: High quality DNA extractions are required for genetic studies. Eight commercially important fish species were chosen for molecular studies. Species and population genetic assessment requires a reliable source of biological material. Different tissues may contain varying amount of DNA (e.g scales, muscles tissues) which may not be equally dealt with by different extraction methods. A number of commercial kits for DNA extraction are available. Routine protocols have also been developed for many different tissues in different species. Yet it is often necessary to establish which is the most appropriate DNA extraction method for a certain tissue in a given research context. In the present study, DNA extracted from eight marine fish species were carried by a standard method using CTAB, a rapid phenol chloroform method, and an advanced method for fish DNA extraction. Quantity of DNA was higher in the standard methods, but adequate quantity and quality of DNA obtained when DNA was extracted by the advanced fish DNA extraction method.
Location: TE 12 New Biology Building
Literature cited 1: Dooley, J.J Sage, H.D., Clarke, M.A., Brown, H.M. and Garrett, S.D., 2005b. Fish species identification using PCR-RFLP analysis and lab-on-chip capillary electrophoresis: application to detect white fish species in food products and an inter laboratory study. J. Agri Food Chem 53 (9): 3348-57. Taggart, J.B. Hynes, R.A., Prodoh, P.A., et al 1992. A Simplified protocol for routine total DNA isolation from salmonid fishes. Journal of Fish Biology 40 (6): 963-965.
Literature cited 2: Tel-Zur, N., Abbos, Myslabodski, D. and Mizrahi, Y. 1999. Modified CTAB procedure for DNA isolation from epiphytic cacti of the genera Hylocereus and selenicereus (cactaceae). Plant Mol Biol Rep. 17: 249.

ID: 59867
Title: SEROTYPE PROFILE AND ACID RESISTANCE OF ESCHERICHIA COLI STRAINS ISOLATED FROM DIFFERENT INDAN FOOD.
Author: Sanjay Chhibber, Sonia B Bhardwaj and Preeti Aghi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 395-398 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: E. coli, E. coli 0157: H7, Acidic food, Hemorrhagic colitis.
Abstract: Most of the outbreaks of hemorrhagic colitis have been related with the consumption of undercooked ground beef or raw milk. Outbreaks involving acidic foods (apple cider, dry fermented sausage and mayonnaise) due to E. coli 0157:H7 have already been reported. But no attempt has been made to look for its prevalence in different Indian foods having low pH. The study was planned to ascertain the incidence of enterohemorrhagic E.coli in different foods being sold in the market. Samples of foods like mango chatni, lemonade, Imli pani, ladoo etc were taken and processed according to standard methods. Sixteen strains of E. coli were isolated and their serotype profile and ability to grow at an acidic pH was determined. The predetermined E.coli serotype was 02 (18.75 %) followed by 018,050 and 0158 (12.50 %) each. However, no enterohemorrhagic E.coli belonging to serotype 0157: H7 was isolated. Acid tolerance studies on acid adapted and non acid adapted strains of enterohemorrhagic E.coli, enteroaggregative E.coli, faecel E.coli and E.coli isolated from urinary tract was evaluated by growing them in foods like yoghurt, sweet lime juice and mango drink. The results better survival of acid adapted enterohemorrhagic E.coli as compared to other strains of E.coli.
Location: TE 12 New Biology Building
Literature cited 1: Anonymous, (centers for Disease control) 1993. Multi-state outbreak of Escherichia coli 0157: H7 infections from hamburgers-western United States. MMWR. 42 (14): 258-263. Basett, J.P. and McClure, 2008. A risk assessment approach for fresh fruits. J. Appl. Microbiol. 104: 925-943.
Literature cited 2: Besser, R.E., Lett Weber, S.M., Doyle, M.P., Barrett, T.J., Wells, J.G. and Griffin, P.M 1993. An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia Coli 0157: H7 in fresh pressed apple cider. JAMA. 269: 2217-2220. Brandl, M.T. 2006. Fitness of human enteric pathogens on plants and implications for food safety. Annu. Rev. Phytopathol. 44: 367-392.

ID: 59866
Title: THE LINK BETWEEN BREAST CANCER AND DIABETES.
Author: Suman Kumar Ray.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 389-394(2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Breast Cancer, Diabetes, Risk factor, Insulin-like growth factor (IGF).
Abstract: There is a consensus that diabetes is associated with a spectrum of breast cancers but the underlying mechanisms are largely unknown. On the other hand, there are ongoing debates about the risk association of insulin use with cancer. We have briefly reviewed recent related research on exploration of risk factors for breast cancer and diabetes and also link between them. Epidemiological studies have shown an association between diabetes mellitus and breast cancer: there is a twofold increased risk for breast cancer in women with late onset diabetes. Hyperinsulenemia and hyperglycemia are biomarkers for insulin resistance. Both of these disorders are critical to the initial development and progression of breast cancer. The hyperinsulinaemia seen in late onset diabetes may promote breast cancer because insulin is a growth factor for human breast cancer cells. At least four mechanisms may associate diabetes mellitus and breast cancer: activation of the insulin pathway, activation of the insulin-like growth factor (IGF) pathway, altered regulation of endogenous sex hormones and altered regulation of adipocytokines.
Location: TE 12 New Biology Building
Literature cited 1: American Diabetic Association. 2012. Diagnosis and classification of diabetes mellitus. Diabetes Care. 35: 64-71. Baliunas, D.O., Taylor, B.J., Irving, H., Roerecke, M., Patra, J., Mohapatra, S. and Rehm, J. 2009. Alcohol as a risk factor for type 2 diabetes: Asystematic review and meta-analysis. Diabetes Care. 32: 2123-2123.
Literature cited 2: Barclay, A.W., Petocz, P., McMillan-Price, J., Flood, V.M., Prvan, T., Mitchell, P. and Brand-Miller, J.C. 2008. Glycemic index, glycemic load, and chronic disease risk-a metaanalysis of observational studies. Am J Clin Nutr. 87: 627-737 Barone, B.B., Yeh, H.C. and Snyder, C.F. 2008. Long-term all-cause mortality in cancer patients with preexisting diabetes mellitus: a systematic review and meta-analysis. Jour of the Ame Med Assoc. 300 (23): 2754-2764.