ID: 59895
Title: EXPERIMENTAL STUDIES ON VERMICOMPOSTING OF ORGANIC FRACTION OF MUNICIPAL SOLID WASTE WITH DAIRY WASTE WATER.
Author: S.P.Ravikannan, G. Manjula and T. Meenambal
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 713-716 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Vermicomposting, Municipal solid waste, Dairy waste water, Effective microorganisms.
Abstract: The dairy industry involves processing raw milk into products such as consumer milk, butter, cheese, yogurt, condensed milk, dried milk (milk powder), and ice cream, using processes such as chilling, pasteurization, and homogenization. Typical by-products include buttermilk, whey, and their derivatives. Since the pollutants generated by the industry are largely lost in production, improvements in production efficiency are recommended to reduce pollutants loads. In this study, a series of experimental studies were conducted with regard to bioconversion of organic fraction of municipal solid waste along with dairy waste water at different C/N ratios using combined composting and vermicomposting technology. The variations in physical and chemical parameters were monitored throughout the process. The results indicate the combined composting and vermicomposting of dairy waste water with municipal solid waste produces high quality compost.
Location: TE 15 New Biology Building
Literature cited 1: Amiya Kumar Prusty, 2011Preparation of silver nanoparticles by microorganism and its application in Pharmacy. International Journal of Biomedical and Advance Research, IJBAR. Arya Vedpriya, 2010. Living Systems: Eco-Friendly Nanofactories. Digest Journal of Nanomaterials and Biostructures. 5 (1): 9.
Literature cited 2: Bijanzadeh, A.R., Vakili, M.R. and Khordad, R. 2012. A study of the Surface Plasmon Absorption band for nanoparticles. International Journal of Physical Sciences. 7 (12): 1943-1948. Ganesh Babu, M.M and Gunasekaran, P. 2009. Production and structural Characterisation of crystalline silver nanoparticles from Bacillus cereus isolate colloids and surfaces B. Biointerfaces. 74: 191-195


ID: 59894
Title: DETERMINATION OF ELECTICAL CONDUCTIVITY, MICRO NUTRIENTS, HEAVY METAL CONCENTRATION AND SPECTRAL ASSIGNMENT STUDY OF THEIR SOIL (RED SOIL)
Author: K.Selva Kothai Nachiyar and B.Annadurai
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 709-712 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Electricical conductivity (EC), FTIR, SEM-EDAX, Atomic Absortption Spectroscopy (AAS).
Abstract: The presence of mineral elements, Heavy metal concentrations in the Red Soil (Their Soil) sample played a vital role for EC variation. The analytical study of elements like Silicon, Aluminium, Carbon, Iron, Thorium Uranium etc. present in the Red soil carried out by SEM-EDAX-SAIF, IIT Chennai. Analysis of the Red soil sample by using AAS observed from CPR Environmental Education Centre, Chennai. The spectral assignment of (FTIR) peaks determined the presence of N-H stretching, C=C stretching, Si-O-Si group and C-Cl stretching etc. The existence of functional groups, mineral elements and Heavy metals in the Red soil sample may enhance EC variation. The EC measurements observed using EC meter. (Model IRI 503).
Location: TE 15 New Biology Building
Literature cited 1: Annadurai, B. 2011. Their soil Reclamation using Tank Silt Tuticorin district. TJEP. (8) 679-683. Dixon, J.B. and Weed, S.B. 1989. Minerals in Soil, Environments, second edition (Madison, wl: Soil Sci. Soc. Amer)
Literature cited 2: Donahue, Miller Shickluna, 1987. Soils an Introduction to Soil and Plant Growth (5th edition) pp. 97-109, pp 297-304. Jawahar, D.et al. 1999b. Fertility capability of their soil of Tamilnadu. J. Indian Soc. Soil Sci. 47 (3): 570-573.


ID: 59893
Title: MANAGEMENT OF SEED DECAYING FUNGI OF ARTOCARPUS HETEROPHYLLUS LAM BY USING AQUEOUS LEAF EXTRACT OF CALYCOPTERIS FLORIBUNDA (ROXB) LAM.
Author: M.Mini and P.M Beebi Razeena.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 705-708 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Biopreservation, Calycopteis Floribunda, Jackfruit, Seed degrading fungi, Seed treatment.
Abstract: The present study focuses on identifying, Isolating and controlling of seed degrading fungi of Artocarpus heterophyllus Lam (Jack fruit in English and Chakka in Malayalam) belonging to the family Moraceae. A bio control method was used for the preservation and enhancing the shelf life period of jack fruit seeds during the period 2012. In vitro studies showed Aspegillus flavus Link; Fr, Aspergillus niger, Van Tiegham, Aspergillus ochraceus Wilhelm, and Fusarium moniliforme. Seeds are treated with Calycopteris floribunda leaf extract (w/v) in vivo showed 100 % control of above degrading fungi and enhancing the shelflife period. It is novel work for bio preservation of seeds.
Location: TE 15 New Biology Building
Literature cited 1: Akinmutimi, A.H. 2006. Nutritive value of Raw and Processed Jack fruit Seed (Artocarpus heterophyllus): Chemical analysis. Agricultural J. 1 (4): 266-271. Bhat, A.V. and Pattabiraman, T.N. 1989. Protease inhibitors from jack fruit seed (Artocarpus in tegrifolia). J. Biosc. 14 (4): 351-365.
Literature cited 2: Booth, C. 1971. The Genus Fusarium. Published by commonwealth mycological Institute Kew, Surrey, England. LIU Jia-Jia, Yang Dong-Liang, Zhang Yan, Yuan Yao, Cao-Fu-Xiang, Zhao Jian-Ming and PengXia-bo, 2009.Chemical component and antimicrobial activity of volatile oil calycopteris floribunda (Roxb). Lam. J. Cent. South Univ. Technol. 16: 0931-0935.


ID: 59892
Title: DETERMINATION OF LEVEL OF CONTAMINATION OF MUTTON PATTIES AND BURGERS MARKETED IN SRINAGAR CITY (INDIA).
Author: Tahir Maqbool, M.Willayat, Irfan A.Mir and Omar Shalil Baba.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 699-703 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Mutton patties, Burgers, Ready-to-eat food products, Food contamination.
Abstract: A study was undertaken to determine the level of contamination of mutton patties and burgers marketed in Srinagar city (India). A total of 60 samples of ready-to-eat foods comprising of 30 mutton patties and 30 burgers were collected from five different zones of Srinagar city viz., east zone, west zone, north zone, south zone, and central zone. The mean bacterial count of mutton patties and burgers, irrespective of the zones was 6.279 and 6.470 log10 cfu/g, respectively. The mean level of contamination in mutton patties in west, north, south, east and central zones was 7.173, 7.000, 6.953, 6.801 and 6.666 log10 cfu/g, respectively. The mean level of contamination in burgers was 7.258, 7.167, 7.053, 6.908 and 6.842 log10 cfu/g in north, west, south, east and central zones, respectively.
Location: TE 12 New Biology Building
Literature cited 1: Aberle, E.D., Forrest, J.C., Gerrard, D.E. and Mills, E.W. 2001. Principles of Meat Science 4th Ed. Hunt Publishing Co, Kendall, USA, pp. 117-154. American Public Health Association (APHA). 1992. Compendium of methods for the microbiological examination of foods. Washington, D.C.
Literature cited 2: Azanza, M.P.V. and Ortega, M.P. 2004. Microbiology of day-old chicks: A Philippine street food. Food Control. 15 (4): 245-252. Banwart, G.J. 1989. Basic Food Microbiology. 2nd Ed. AVI-Van Nostrand Reinhold, New York, N.Y, pp. 11-12.


ID: 59891
Title: STIMULATION OF EXTRACELLULAR PHYTASE PRODUCTION FROM BACILLUS SUBTILIS BY POWDERED RICE BRAN IN SLURRY FERMENTATION.
Author: R.Agalya Devi, Rahul Kumar and K.Balasrishnan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 691-697 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Slurry fermentation, Bacillus subtilis, Rice bran, Phytase
Abstract: Phytase production profile of Bacillus subtilis was investigated here in submerged fermentation with and without enhanced level of fortification by powdered rice bran 9Slurry fermentation). Incorporation of rice bran as substrate particles (5% w/v) in nutrient broth resulted in 5. 11 times better yield of phytase when compared with un-supplemented broth. Among the different substrate particles introduced, rice bran was found to be inducing better phytase productivity. Substrate incorporation at varied concentrations (between 1%-15 % w/v) gave better phytase titers at 9% w/v (41.94 U/mL) of incorporation and 32 hrs of harvest. Acidic medium pH, addition of surfactants after sterilization and bioprocess carried out at 40 ? C were al found to have positive influence on the enzyme titers in the fermentation samples. Suitability of slurry fermentation for phytase production established. Higher enzyme activity at 40 ? C were all found to have positive influence on the enzyme titers in the fermentation samples. Suitability of slurry fermentation for phytase production established. Higher enzyme activity at 40 ? C were al found to have positive influence on the enzyme titers in the fermentation samples. Suitability of slurry fermentation for phytase production established. Higher enzyme activity at 40 ? C and acidic medium pH reiterate the suitability of this phytase as feed premix for poultry.
Location: TE 12 New Biology Building
Literature cited 1: Anderson, R.J. 1914. A contribution to the chemistry of phytin.J.Biol.Chem.17: 171-190. Angelis, M.D., Gallo, G., Corbo, M.R., McSweeney, P.L.H., Faccia, M., Giovine, M. and Gobbetti, M. 2003. Phytase activity in sourdough lactic acid bacteria: purification and characterization of a phytase from Lactobacillus sanfranciscensis CBI. Int. J. Food. Microbiol. 87: 259-270.
Literature cited 2: Asheh, S.A. and Duvnjak, Z. 1995. The effect of Phosphate concentration on phytase production and the reduction of phytic acid content in canola meal by Aspergillus carbonarius during a solid-state fermentation process. Appl. Microbiol. Biotechnol.43: 25-30. Azeke, M.A., Greinier, R. and Jany, K.D. 2011. Purification and characterization of two intracellular phytases from the tempeh fungus Rhizopusoligosporus. J. Food. Biochem. 35: 213-227.


ID: 59890
Title: STUDIES ON CHLORPYRIFOS DEGRADATION USING GC AND FTIR BY KOCURIA SPECIES.
Author: Nagavardhanam Neti and Vishnu Vardhan Zakkula.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 685-689 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Chloropyrifos; Kocuria sp; GC; FTIR.
Abstract: An organophosphate (OP) pesticide is the most popular type of pesticide family, which effectively eliminates pests owing to its acute neurotoxicity. Organophosphorus hydrolase (oph) is a bacterial enzyme that is capable of degrading a wide range of neurotoxic OP pesticides. It was proved that Kocuria species was capable in degrading organophosphate (OP) pesticide. The present study was aimed at analysing its ability in degrading chloropyrifos using GC and FTIR.
Location: TE 12 New Biology Building
Literature cited 1: Akhtar, S. and Ahmed, A. 2002. Pesticides human health and ecosystem. Journal of Baqai Medical University. 5 (2): 16-19. Baskaran, S., Kookana, R.S. and Naidu, R. 2003. Contrasting behavior of chlorpyrifos and its primary metabolite, TCP (3, 5, 6-trichloro-2pyridinol), with depth in soil proules. Australian Journal of Soil Research. 41: 749-760
Literature cited 2: Chungjatuponchai, W. and Fa-Aroonsawat, S. 2008. Biodegradation of organophosphate pesticide using recombinant Cyanobacteria with surface-and intracellular-expressed organophosphorus hydrolase. Journal of Microbiology and Biotechnology. 18 (5): 946-951. Feng, Y., Racke, K.D. and Bollag, J.M. 1998. Isolation and characterization of a chlorinated pyridinol degrading bacterium. Applied Environmental Microbiology 63: 4096-4098.


ID: 59889
Title: ENHANCEMENT OF 1, 3-PROPANEDIOL PRODUCTION IN LACTOBACILLUS REUTEI BY FED-BATCH CULTIVATION.
Author: Hema Vaidyanathan, Pandiaraj Suppuram, Venkatesh Endalur Gopinarayanan, Yuvaraj Purushothaman and Subramanian Ramalingam.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 679-684 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: L.reuteri, 1, 3-Propanediol, Fed-batch, Cell density, Product tolerance.
Abstract: There is an urgent need for the production of valuable chemicals like 1,3- Propanediol from glycerol, a waste product from biodiesel industry. Since 1, 3-Propanediol has biomedical and healthcare applications, it would be suitable to procedure it in an organism with wide acceptability like Lactobacillus reuti. However, lower cell density as well as reduced product titres seem to be the key factors limiting process development. To address these issues, a fed-batch strategy has been presented in the current study. A 4-fold increase in biomass and 25 % increase in biomass and 25 % increase in 1, 3-PD concentration could be achieved in fed-batch as compared to the batch mode of fermentation. Product tolerance assay has revealed that L. reuteri is capable of tolerating 100 g/L of 1,3Propanediol, indicating its potential for industrial application.
Location: TE 12 New Biology Building
Literature cited 1: Baeza-Jimenez, R., Lopez-Martinez, L.X., De la Cruz-Medina. J., Espinosa-de-los-Monteros, JJ and Garcia-Galindo, H.S 2011. Effect of glucose on 1, 3-Propnediol Production by Lactobacillus reuteri. Revista Mexicana de Ingenieria Quimica. 10: 39-46. Celinska, E. 2010. Debottlenecking the 1, 3-Propanediol pathway by metabolic engineering. Biotechnol Adv. 28: 519-530.
Literature cited 2: Drogdgynska, A., Leja, K. and Czaczyk, K. 2011. Biotechnological production of 1, 3-Propanediol from crude glycerol. Journal of Biotechnology, Computational Biology and Bionanotechnology. 92 (1): 92-100 El-Ziney, M.G., Arneborg, N., Uyttendaele, M., Debevere, J. and Jakobsen, M. 1998. Chracterization of growth and metabolite production of Lactobacillus reuteri during glucose/glycerol cofermentation in batch and continuous cultures. Biotechnol Lett. 20: 913-916.


ID: 59888
Title: CHANGES IN SOIL ENZYME ACTIVITIES UNDER THE INFLUENCE OF COTTON GINNING INDUSTRY EFFLUENTS.
Author: K.Venkateswar Reddy, T. Vijayalakshmi, Kodaparthi Ashwitha and E.Prathibha Shalini.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 673-678 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Cotton gining industry, Effluents, Physicochemical, Biological, Soil enzymes.
Abstract: Release of cotton ginning industry effluents on the agricultural lands causes and indicative change in nutrient cycling and organic matter processing. In the present study, cotton ginning industry effluent discharged soil (test) and undischarge soil (control were collected from the surrounding areas of cotton ginning industry. The physicochemical, biological properties and soil enzyme activities such as amylase. Protease, cellulose and urease were examined. The experimental results indicated that, most of the physicochemical properties such as silt, clay, electrical conductivity, water holding capacity, organic matter and total nitrogen, phosphorus and potassium, microbial population and selected enzyme activities were significantly higher in the test sample than in the control. Additionally, activities were increased with increasing the incubation period up to 21 d over d, however, activities were adversely affected at 28 d. Furthermore, relatively higher activities were observed in soil incubated in the presence of substrate than in the absence of substrate.
Location: TE 12 New Biology Building
Literature cited 1: Alexander, M. 1961. Introduction to soil Microbiology. Wiley Estern Ltd. New Delhi. Andrade, M.L., 2002. Industrial Impact of Marsh Soils at the Bahia Blance Ria, Argentina. Journal of Environmental Quality. 31: 532-538.
Literature cited 2: Bollag, J.M., Berthelin, J., Adriano, D. and Huang, P.M.2002. Impact of soil minerals-organic component - microorganisms interactions on restoration of terrestrial ecosystems. Poster presentation, paper no. 861, Symposium no.47. 17th WCSS. Burns, R.G., L.J., Gregory, G. Lethbridge, and N.M. Pettit. 1978. The effect of irradiation on soil enzyme stability. Exprerientia.


ID: 59887
Title: RAPID MOLECULAR DETECTION AND IDENTIFICATION OF AFLATOXINS PRODUCINGFUNGAL GENE, AFLQ, IN INFCTED MAIZE SAMPLES USING NORMAL PCR.
Author: Nagalakshmi S, Manorama K, Anurag Chathurvedi, Sreedhar M, Krishna Bhagavatula MR, Sunil-Chidambar K, Ravicharan A, Prathima N, Durgarani Ch. V and Reddy Vln.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 667-671 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Maize, Aflotoxins and PCR
Abstract: Maize (Zea mays L.) is one of the cereals which serves as a main source of food, forage and processed products for industry with a production of around 790 million tones. As a staple food it provides more than one-third of the calories and proteins in some countries. Aflatoxins are a group of chemicals produced by certain mold fungi such as Aspergillus flavus and Aspergillus parasiticus. Aflatoxins are harmful or fatal to livestock and human beings as they are carcinogenic. The present study was undertaken with the main aim of detecting the presence of these genes encoding the production of mycotoxin in the infected maize samples. One hundred and thirty maize samples infected with the fungi Aspergillus parasiticus and Aspergillus flavus were collected from the markets, godowns and farmers ' fields. DNA isolated from such maize samples was amplified in PCR using four sets of forward and reverse gene specific primers designed using DNASTAR Lasergene 8.0 version software from original gene sequences (obtained from GENBANK) of the specific gene, aflq. The fragments obtained were resolved on the bioanalyzer, DNA 1000 Labchip, for generating data on the size of amplified fragments. A 168 base pair fragment of aflq target sequence was amplified in twenty nine maize samples. This study helps in easy detection of mycotoxins present in the contaminated samples in storage which are unfit for consumption thereby avoiding the hazardous influence of such toxins on human and animal health.
Location: TE 12 New Biology Building
Literature cited 1: Bottalico, A. 2009. Aspergillus diseases of cereals: species complex and related mycotoxin profiles, in Europe. Journal of Plant Pathology. 80: 85-103. Niessen, L. 2007. PCR based diagnosis and quantification of mycotoxin fungi. International Journal of Food Microbiology. 119: 38-46.
Literature cited 2: Russell, R. and Paterson, M. 2006. Identification and quantification of mycotoxigenic fungi by PCR. Process Biochemistry. 41 (7): 1467-1474. Strange, R.N. and Scott, P.R. 2006. Plant Disease; a threat to global food security. Annual Review of Phytopathology. 43: 83-116.


ID: 59886
Title: UTILIZATION, ISOLATION AND CHARACTERIZATION OF CHLORELLA VULGARIS FOR CARBON SEQUESTRATION AND WASTE WATER TREATMENT BY PERFORMING FTIR SPECTRAL STUDIES.
Author: P.Indhumathi, M.Soundararajan, P.S. Syed Shabudeen, U.S. Shoba and E. Suresh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 661-666 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Chlorella vulgaris, uv-visible , FTIR, SEM, Nitrate removal, Phosphate removal.
Abstract: Green microalgae Chlorella vulgaris species was collected from Bhavani Lake at Erode District, Tamil Nadu State, India. The Fourier Transform Infrared spectroscopy and Scanning Electron Microscopic studies were utilized to examine morphology of Chlorella vulgaris is attempted for its efficiency in removing nitrate and phosphate from waste water medium. These experimental studies reveal that, ' Chlorella vulgaris ' is having full-fledged efficiency in removing nitrates and phosphates at a time intravel of 6 days.
Location: TE 12 New Biology Building
Literature cited 1: Anderson, R.A. 2005. Algal Cultural Techniques, Elsevier academic press, London. Becker, W. 2004. Microalgae in human and animal nutrition. In: Richmond, A editor. Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Blackwell Publishing, 312-346.
Literature cited 2: Benning, L.G., Phoenix, V.R., Yee, N. and Tobin, M.J. 2004. Molecular characterization of cynobacterial silification using synchrotron infrared microspectroscopy. Geocimica et Cosmochimica Acta. 68: 729-741. Basterj, Korting, H.C., Traenkle, P. and Schmalreck, A.F. 1999. Identification of dermatophytes by Fourier Transform Infrared Spectroscopy, Mycoses ISSN 09333.42: 525-528.


ID: 59885
Title: IDENTIFICATION OF DRUG-RESISTANT BACTERIA AMONG EXPORT QUALITY SHRIMP SAMPLES IN BANGLADESH.
Author: Tasnia Ahmad, Sagar Baidya, Basudeb Chandra Sharma, Mushfia Malek, Kamalkanta Das, Mrityunjoy Acharjee, Saurab Kishore Munshi and Rashed Noor.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 655-660(2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Export quality shrimp, Pathogens, Antibiotic resistance, Microbiological quality, Consumer safety.
Abstract: Present study attempted to detect the pathogenic load n export quality shrimp samples and to demonstrate the drug resistance traits of the pathogenic isolates. Samples were found to be contaminated with Listeria spp. (1.0 x 105 cfu/g), Staphylococcus aureus (9.1 x 106 cfu/g) and Escheria coli (1.0 x 103 cfu/g). Study of antibiogram revealed that Listeria spp. was resistant against penicillin G and ampicillin, S. aureus against trimethoprim, erythromycin, sulfamethoxazole, and Escherichia coli against erythromycin and polymixin B. However, all the isolates were found to be susceptible against tetracycline, and most of them also exhibited a greater degree of susceptibility towards amoxicillin, ceftriaxone, ciprofloxacine, streptomycin, chloramphenicol and cefixime. The antibacterial activity activity of the shrimp blend was also examined. Interestingly, the head and body portions of the shrimp samples exhibited antibacterial activity against Staphylococcus spp. and Listeria spp.
Location: TE 12 New Biology Building
Literature cited 1: Alfrad, E.B. 2007. Bensons microbiological applications. McGraw-Hill Book Company, New York. Allerberger, F. and Mittermayer, H.2008. Antimicrobial stewardship. Clinical Microbiology and Infection. 14 (3): 197-199.
Literature cited 2: Antony, M.M, Jeyasekaran, G., Shakila, R.J. and Shanmugam, S.A. 2002. Microbiological quality of raw shrimps processed in seafood processing plants of Tuticorin, Tamil Nadu, India. Asian Fisheries Science. 15 (1): 33-41. Bauer, A., W., Kirby, W.M.M., Sherris, J.C. and Tierch, M. 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology. 45 (4) : 493-496.


ID: 59884
Title: MICROBIOLOGICAL STUDY OF DRINKING WATER: QUALITATIVE AND QUANTITATIVE APPROACH.
Author: Tasnia Ahmed, Mrityunjoy Acharjee, Md. Shohanur Rahman, Monirunnessa Meghla, Janifar Jamal, Saurab Kishore Munshi and Rashed Noor.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 647-654 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: MPN (Most Probable Number) Fecal contamination, Indicator bacteria, Pathogens, Drinking water.
Abstract: Consumption of drinking water contaminated with focally originated pathogenic bacteria is mostly responsible for the onset of water borne diseases outbreaks especially in developing countries. Current study attempted to analyze 25 treated drinking water samples both qualitative-and quantitatively from different areas of Dhaka metropolis, Bangladesh where 90 % cases of diseases (dysentery, typhoid, Cholera and diarrhea) have long been reported due to the water borne microorganisms diseases. Through the most probable number (MPN) method, 5 samples out of 25 were found to be non-potable as they had been contaminated with Escherichia coli indicating the risk for fecal contamination responsible for diseases outbreaks. Other contaminating Gram negative bacteria were characterized as Klebsiella spp, Alcaligenes faecalis, Pseudomonas spp. and Aeromonas spp. Intrestingly the presence of E.coli was detected in the same 5 samples within a range of 3.0 x 102 to 1.7 x 105 cfu/ml by the conventional cultural and biochemical methods. Moreover, a huge array of other pathogenic bacteria was also detected (5.0 x 102 -3.5 x 108 cfu/mL) through this method. Further detection of drug resistance traits among the bacterial isolates would be of public health significance.
Location: TE 12 New Biology Building
Literature cited 1: Abera, S., Zeyinudin, A., Kebede, B., Deriew, A., Ali, S.and Zemene, E. 2011.Bacteriological analysis of drinking water sources. Afr. J. Microbiol. Res. 5 (18): 2638-2641. Acharjee, M., Jahan, F., Rahman, F. and Noor, R. 2013. Bacterial proliferation in municipal water supplied in Mirpur locality of Dhaka city, Bangladesh. Clean Soil, Air, Water, DOI: 10. 1002./clen.201200618
Literature cited 2: Acharjee, M., Rahman, F., Beauty, S.A., Feroz, F., Rahman, M.M. and Noor, R. 2012. Microbiological study on supply water and treated water in Dhaka city, S.J.Microbiol. 1 (1): 42-45. Alfrad, E.B. 2007. Bensons Microbiological Applications. Mcgraw-Hill Book Company, New York.


ID: 59883
Title: A REVIEW OF THE EFFECTS OF BALCONY ON INDOOR VENTILATION PERFORMANCE.
Author: Fatemeh Mozaffari Ghadikolaei, Dilshan Remaz Ossen and Mohd Farid Mohamed
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 639-645 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Balcony, Natural ventilation, Multi storey building.
Abstract: Incorporating airflow principles and techniques, and applying them in building designs can create comfortable indoor environments. Fa?ade characteristics, especially near the opening wall, can change airflow patterns and airspeed inside buildings. Balcony is an example of these features that can be used in front of openings to control the performance of indoor ventilation. Through literature review, this paper looks into the effeteness of the provision of balconies to induce natural ventilation in building. This paper reviews several studies on airflow patterns inside and outside buildings in single-sided and cross ventilation strategy as well as the impact of wind incidents, building height and balcony configuration on ventilation performance. The outcome of this study demonstrates that architects can incorporate balcony as a green design element to improve indoor natural ventilation.
Location: TE 12 New Biology Building
Literature cited 1: Ai, ZT, CM Mak, JL Niu, and ZR Li. 2011. The assessment of the performance of balconies using computational fluid dynamics. Building services Engineering Research and Technology 32 (3): 229-243. Ai, ZT, CM Mak, JL Niu, and ZRLi. 2011. Effect of balconies on thermal comfort in wind-induced, naturally ventilated low-rise buildings. BuildingServices Engineering Research and Technology 32 (3): 277-292.
Literature cited 2: Ai, ZT, CM Mak, JL Niu, ZR Li, and Q Zhou.2011. The Effect of Balconies on Ventilation Performance of Low-rise Buildings. Indoor and Built Environment20 (6): 649-660. Boutet, T.S. 1987. Controlling Air Movement: A Manual for Architects and Builders: McGraw-Hill.


ID: 59882
Title: WOUND HEALING ACTIVITY OF COLEUS AROMATICUS IN EXPERIMENTALLY INDUCED DIABETIC MICE.
Author: Kaleshware Muniandy, Zaiton Hassan and Mohd Hafez Mohd Isa
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 627-638 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Wound healing, Coleus aromaticus, Excision wound model, Dead space wound model, Granulation tissue, Epithelialization.
Abstract: Background. The ethanolic and aqueous extract of C. aromaticus leaves and roots (100 mg kg-1 day-1 for 10 days) were evaluated for its wound healing activity in Monosodium Glumate induced diabetic mice using excision and dead space wound models. Excision wound model was performed on five different groups of mice to measure the progress of wound area for ten consecutive days. Dead space wound model was performed on four different groups of mice to estimate wet and dry granulation tissue as well as to examine the histological appearances of granulation tissue after ten days. Ethanolic extract treated wounds was found to show 76.6 % of wound area reduction when it is compared with controls that exhibited 55.9 % of wound area reduction. The ethanolic extract treated wound had epithelised faster compared to controls. The wet and dry granulation tissue weight has increased significantly in extract treated mice when it is compared with the controls. Extract treated mice exhibited good proliferation of collagen tissues with satisfactory angiogenesis. Coleus aromaticus promotes significant wound healing in induced diabetic mice and evaluation of this activity on human organism is highly suggested.
Location: TE 12 New Biology Building
Literature cited 1: Adam, J., Singer Richard and Clerk, A.F.1999. Cutaneous wound healing. The New England Journal of Medicine. 1(1): 738-746. Ahmad Oryan, Aboutorab T. Naeini and Behrooz Nikhaval, 2010. Effect of aqueous extract of Aloe vera on experimental cutaneous wound healing in rats. Veterinarski Arhiv. 80 (4): 509-522.
Literature cited 2: Ailsa Sharp and Jane Clark, 2011. Diabetes and its effects on wound healing. Nursing Standrd. 25 (45): 41-47. Anand K. Jain, Ashish Dixit and Swaroop C. Mehta, 2012. Wound healing activity of aqueous extract of leaves and roots of Coleus aromaticus in rats. Acta Poloniae Pharmacutica-Drug Research. 69 (6): 1119-1123.


ID: 59881
Title: PRODUCTION, PARTIAL PURIFICATION ND BIOCHEMICAL CHARACTERIZATION OF POLYPHENOL OXIDASE FROM TRICOTHECIUM SP (ENDOPHYTIC FUNGUS OF COCOA PODHUSK)
Author: Sartini, ABD.Rauf Patong, Tjodi Harlim and Pirman
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 621-626 (2013)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Polyphenol oxidase, Tricothecium sp, Endophytic fungus, Theobroma cacao.
Abstract: Fungal strains are considered to be excellent sources for industrial polyphenol oxidase production. Polyphenol oxidase could be produced by endophytic fungus Trichothecium sp from podhusk of Theobroma cacao L. The aim of this research is to partial purify and characterize of the enzyme. The enzyme was produced by submerged fermentation using media with composition, i.e. 1% yeast extract, 25 ppm Cuso4.5H2O, 0.34 % gallic acid, 0.1% KH2PO4, 0.05 % MgSO4.7H2O. The steps of purification were done by 60-90 % (NH4)2SO4 precipitation, dialyzed, then partial purified by gel filtration chromatography using sephadex G-100, respectively. Polyacrylmide gel electrophoresis of the polyphenol oxidase showed 2 bands. Biochemical characteristics of the polyphenol oxidase are laccase and tyrosinase category. Opimum temperature and pH are 30 ? C and 7.0, respectively. The enzymes obeys Michealis-Menten kinetic with Km and Vmax values being 0.625 mM and 2083 U/mL, catechol as substrate.
Location: TE 12 New Biology Building
Literature cited 1: Brijwani, K., Rigdon, A. and Vadlani, P.V., 2010. Fungal Laccases: Production, Function and Applications in Food Processing. Enzyme Research. 11 pages. Chikezie, P.C. 2006. Extraction and Activity of Polyphenol Oxidase from Kolanuts (Cola nitida and Cola acuminate ) and Cocoa (Theobroma cacao). J.Agricul. and Food Sci.4
Literature cited 2: Duran, N. and Esposito, E. 2001. Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review. Applied Catalysis B: Environmental 28: 83-99. De Faria, R, O. de Moure, R.O Amazonas, V.R., Krieger, A.M.R.L and Mitchell, D.A. 2007. The biotechnological potential of mushroom tyrosinase. Food Technology and Biotechnology. 45 (3): 287-294.