ID: 59910
Title: MOLECULAR DETECTION OF OCHRATOXIN PRODUCING FUNGAL GENE, POLYKETIDE SYNTHASE GENE (PKS) IN INFECTED MAIZE SAMPLES USING PCR.
Author: Nagalakhshmi S, Manorama K, Anurag Chaturvedi , Shreedhar M, Krishna Bhagavatula Mr, Sunil-Chidambar K, Ravicharan A, Prathima N, Durgarani Ch. V and Reddy Vln.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 789-793 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Maize, Ochratoxin, Bioanalyzer and Normal-PCR
Abstract: Corn production was estimated a production of around 790 million tones. Most of the corn is used for feed, food, and seed, maize (Zea mays L.) is one of the cereals which serves as a main source of food, forage and processed products for industry. As a staple food it provides more than one-third of the calories and proteins in some countries. Ochratoxins are cyclic polyketides, the most potent of which is ochratoxin A (OTA). OTA has been associated with human endemic neuropathy. Large number of other Penicillium and Aspergillus species have since been reported to be OTA producers. The present study was undertaken with the main aim of detecting the presence of the genes encoding the production of mycotoxin, namely, ochratoxin in the infected maize samples. One hundred and thirty maize samples infected with the fungi Aspergillus and Penicillium were collected from the markets, godowns and farmers ' fields. DNA isolated from such maize samples was amplified in PCR using sets of forward and reverse gene specific primers designed using DNASTAR Lasergene, pks. Fragments. A 200 base pair fragments were amplified in eleven samples. This study helps in easy detection of mycotoxins present in the contaminated samples in storage which are unfit for consumption thereby avoiding the hazardous influence of such toxins on human and animal health.
Location: TE 15 New Biology Building
Literature cited 1: Abe, Y., Suziki, T., Mizuno, T., Ono, C., Iwamoto, K., Hosobuchi, M. and Yoshikawa, H. 2002. Effect of increased dosage of the ML-236B (compactin) biosynthetic gene cluster on ML-236B production in penicillium citrinum. Mol Genet Genomics. 268: 130-137. Bottalico, A. 1998. Fusarium diseases of cereals: species complex and related mycotoxin profiles, in Europe. Journal of Plant Pathology. 80: 85-103.
Literature cited 2: Brown, D.W., McCormick, S.P., Alexander, N.J., Proctor, R.H. and Desjardins, A.E. 2001. A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and Fusarium graminearum? Fungal Genetics and Biology 32: 121-133. Chandler, E.A., Simpson, D.R., Thomsett, M.A. and Nicholson, P. 2003. Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes and characterization of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis. Physiological and Molecular Plant Pathology.


ID: 59909
Title: ISOLATION OF POTENTIAL MICROBIAL PRODUCERS OF FRUCTOSYL TRANSFERASE FROM BAGGASSE AND SELECTED SOIL SITES OF CHHATTISGARH, INDIA.
Author: Seema A. Belorkar, A.K. Gupta and Vibhuti Rai.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 785-788 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Fructosyl transferase, Fructooligosaccharides and aspergillus niger.
Abstract: The present study focuses on microbiological ecology of varied soil samples which can be exploited as potential sources for isolation of industrially important microbes. Seven soil samples and one baggasse sample was selected as sources to isolate microbes which were screened further for production of Fructosyltransferease (Ftase) enzyme. Ftase is an enzyme tool for synthesis of Fructooligosaccharides (FOS). The undertaken study revealed that there is a significant variation in the average number of bacteria, molds and yeasts in various samples. The differences in their means were found to be statistically insignificant. The counts of microbes per gram of samples were found to be in expected proportions indicating that differences in sampling locations do not change the relative proportions indicating that differences in sampling locations do not change the relative proportions indicating that differences in sampling locations do not change the relative proportions of the types of microbes. The present study will also prove to be guideline for selection of sampling sites for future isolations intended towards Fructosyltransferease producing microbes.
Location: TE 15 New Biology Building
Literature cited 1: Amir, H. and Pineau, R. 1998. Influence of plants and cropping on microbiology characteristics of some new Caledonian Ultramafic soils. Aust. J. Soil Res. 36: 457-470. Atlas, R.M., Bartha, R. 1998. Microbial Ecology: Fundamentals and Applications. 4th Edition, Benjamin Cummings Publishing Company Inc., Addison, Wesley Longman Inc., pp. 300-350.
Literature cited 2: Cho, W.T., Lim, J.Y. and Lee, S.S. 1990. Isolation and identification of the black Yeast producing Fructosyl transferase. Kor. J. Mycol. 18: 20-25. Fawkia, M.B., Fattah, A.M.A., Hasanein, D.A., Mustafa, F.A.and Fatta, F.FA. 2009. Production and Some Properties of Fructosyltransferease from Bacillus Cereus. Applied Sciences Research. 5: 1132-1141.


ID: 59908
Title: INFLUENCE OF CATHODE ELECTRON ACCEPTORS AND ANODE MICROBES IMPROVING ELECTRICITY GENERATION IN DUAL-CHAMBERED MICROBIAL FUEL CELL USING DISTILLERY WASTEWATER.
Author: K.Geetha and S.Amal Raj.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 779-784 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Distillery Wastewater, Salt bridge, Mixed consortium, Electron acceptor, Dissolved oxygen.
Abstract: In this investigation a dual-chambered microbial fuel cell (MFC) with a salt bridge separator as a proton exchange membrane (PEM) was used to harness bioelectricity from primary treated distillery wastewater. The study examines the activity of various cathodic electron acceptors using chemicals such as potassium permanganate (KMnO4), potassium ferricyanide (K3FeCN6) and dissolved oxygen (DO) in a cathode chamber. Among the three different electron acceptors, KMnO4 possesses high oxidation capacity, which results in a maximum power output of 6.26 m W/m2 than K3FeCN6 (1.280 mW/m2) and dissolved oxygen (0.83 m W/m2). The biocatalytic activity of the microbial community for electron transport from the substrate to the anode electrode were also studied using a strain (SS) and distillery-enriched mixed consortium (MC) based on the cell potential. Compared to the single strain the mixed consortium consumes more complex carbon sources and accepts wider substrates, which increases the cell potential.
Location: TE 15 New Biology Building
Literature cited 1: APHA. 1998. Standard Methods for Examination of Water and Wastewater, 20th ed. American Public Health Association/ American Water Works Association/ Water Pollution Control Federation, Washington, DC. Behera, M. and Ghangrekar, M.M. 2009. Performance of microbial fuel cell in response to change in sludge loading rate at different anodic feed pH. Bioresour. Technol. 100 (21): 5114-5121.
Literature cited 2: Chae, K.J., Choi, M.J., Lee, J.W., Kim, K.Y. and Kim, I.S. 2009. Effect of different substrates on the performance, bacterial diversity, and bacterial viability in microbial fuel cells. Bioresour. Technol. 100 (120: 3518-3525. Chang, I.S. Jang, J.K., Gil, G.C., Kim, M., H.J. Cho, B.W. and Kim, B.H. 2004. Continuous determination of biochemical oxygen demand using microbial fuel cell type Biosensor. Biosens Bioelectron. 19 (6): 607-613


ID: 59907
Title: POTENTIATION OF ANTIBIOTIC-OXACILLIN BY CITRIC ACID AGAINST METHICILLIN RESISTANT STAPHYLOCOCCI.
Author: N.A. Chandak, B.J.Wadher and S.R. Deshmukh
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 773-778 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Methicillin Resistant Staphylococci, Potentiation, Citric acid.
Abstract: In recent years, the environmental contamination caused by excessive use of chemical pesticide increased the interests in integrated pest management, where chemical pesticides are substituted by bio-pesticides to control plant pests diseases. Trichoderma viride is an ecofriendly biological fungicide. In the present study different types of polished and unpolished rice were used to prepare rice wash for cultivation of Trichoderma viride and its maximum yield were recorded in rice wash of unpolished rice and the lowest yield in polished rice. The cultivated Trichoderma viride were used as pesticide and its field trials were conducted on one Acre of land. Trichoderma viride enhanced the root and plant development, solubilisation of inorganic nutrients and induced resistance inactivation of pathogens enzymes might be due to secreting certain plant growth stimulating substances.
Location: TE 15 New Biology Building
Literature cited 1: Alagarsamy, G. and Sivaprakasam, K. 1988. Effect of antagonists in combination with carbendazim against Macrophomina phasiolina infection in cowpea. Journal of Biol.Control. 2: 123-125. Aneja, K.R. 2003. Experiment in Microbiology, Plant Pathology and Biotechnology (4th Edition), New Age International Publication.
Literature cited 2: Bailey, D.J., Kleczkowski, A. and Gilligan, C.A. 2004. Epidemiology dynamics and the efficiency of biological control of soil-borne diseases during consecutive epidemics in a control environment. New Phytopathology. 161: 569-576. Chaudhari, P.J., Shrivastava, P. and Khadse, A.C. 2011. Substrate evaluation for mass cultivatrion of Trichoderma viride. Asiatic Journal of Biotechnology Resources. 2 (4): 441-446.


ID: 59906
Title: CULTIVATION OF TRICHODERMA SPECIES ON RICE WASH AND ITS APPLICATION IN PEST CONTROLLING.
Author: P.B. Arekar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 769-772 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Trichoderma viride, Rice wash, Cotton plant.
Abstract: In recent years, the environmental contamination caused by excessive use of chemical pesticides increased the interests in integrated pest management, where chemical pesticides are substituted by bio-pesticides to control plant pests and plant diseases. Trichoderma viride is an ecofriendly biological fungicide. In the present study different types of polished and unpolished rice were used to prepare rice wash for cultivation of Trichoderma viride and its maximum yield were recorded in rice wash of unpolished rice and the lowest yield in polished rice. The cultivated Trichoderma viride were used as pesticide and its field trials were conducted on one Acre of land. Trichoderma viride enhanced the root and plant development, solubilisation of inorganic nutrients and induced resistance inactivation of pathogens enzymes might be due to secreting certain plant growth stimulating substances.
Location: TE 15 New Biology Building
Literature cited 1: Alagarsamy, G. and Sivaprakasam, K. 1988. Effect of antagonists in combination with carbendazim against Macrophomina phasiolina infection in cowpea. Journal of Biol. Control. 2: 123-125. Aneja, K.R. 2003. Experiment in Microbiology, Plant Pathology and Biotechnology (4th Edition), New Age International Publication.
Literature cited 2: Bailey, D.J., Kleczkowski, A. and Gilligan, C.A. 2004. Epdermiological dynamics and the efficiency of biological control of soil-borne diseases during consecutive epidemics in a control environment. New Phytopathology. 161: 569-576. Chaudhari, P.J., Shrivastava, P. and Khadse, A.C. 2011. Substrate evaluation for mass cultivation of Trichoderma viride. Asiatic Journal of Biotechnology Resources. 2 (4): 441-446.


ID: 59905
Title: ASSESSMENT OF DIFFERENT PARTS OF ANNONA SQUAMOSA L. FOR THEIR ANTIMICROBIAL ACTIVITY AGAINST PATHOGEN ISOLATES FROM RAIPUR REGION.
Author: Rinu Khan and A.K. Gupta
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 765-768 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antimicrobial activity, Agar diffusion method, Gram-negative pathogens, Gram positive pathogen, Annona squamosa, Leaf, Stem, Root.
Abstract: Annona Squamosa L. member of the Annonaceae family, also known as custard apple, is commonly found in deciduous forests and is cultivated in wild in various parts of India. In this investigation, antimicrobial effect of root, seed and leaves extracts of Annona squamosa L. on clinical isolates of three Gram negative and one Gram positive pathogens from Raipur Region were studied, using paper disc method. Gram negative and positive microorganisms studied were Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus. For examination of antimicrobial properties of A. squamosa aeruginosa and Staphylococcus aureus. For examination of antimicrobial properties of A. squamosa on pathogenic microorganisms, disc diffusion method using different parts of the plant extracted with four different solvents namely petroleum ether, acetone, ethyl alcohol and water were used. Among all, the root of Annona squamosa was found to be more pronounced in Pseudomonas aeruginosa (7.66 ? 0.33) for the acetone extraction of root of Annona squamosa was found to be more effective as compared from seed and leaf. The zone of inhibition was found to be more pronounced in Pseudomonas aeruginosa (7.66 ? 0.33) for the acetone extraction of root of A. squamosa followed by Escherichia coli (6.66 ? 1.20), S. aureus (4.66 ? 0.88) and Klebsiella pneumonia. Seeds of A. squamosa also showed similar pattern of inhibitory effects on tested organism lesser than that of root. The leaf of this plant showed lower antimicrobial potential compared from root and seed. Aqueous extract did not express satisfactory result against clinical isolates. The activity index of root of A. squamosa was found to be greater as compared to the activity index of the seed and leaf. The phytochemical analysis revealed that the plant is rich in chemicals such as alkaloids, flavonoids, saponins, sterols, tannins and terpenoids. The results suggested that acetone could be effectively used for the extraction of phyto compounds from A. squamosa. Similarly the root of the plant could be used as important source for antimicrobial property against clinical isolates.
Location: TE 15 New Biology Building
Literature cited 1: Basri, D.F., Sharif, R., Morat, P. and Latip, J. 2005. Evaluation of antimicrobial activities of the crude extracts from Garcinia atroviridis and Solanum torvum. Malaysian J. Sci, 24: 233-238. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck, M. 1996. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45: 493-496.
Literature cited 2: Betoni, J.E.C., Mantovani, R.P., Barbosa, L.N., Di-Stasi, L.C. and Fernandes, A. 2006. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases.Mem.Inst. Oswaldo Cruz. 101 No. 4. De-Omena, M.C., Bento, E.S., De Paula, J.E. and Sant Ana, A.E.G. 2006. Larvicidal diterpens from Pterodon Polygalaefloras. Vector Borne and Zoonotic Dis. 6: 216-222.


ID: 59904
Title: EVALUATION OF GENETIC DIVERSITY IN SOME BANANA HYBRIDS USING RAPD MARKERS.
Author: Sukhen Chandra Das and T.N Balamohan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 759-764 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Hybrids, Cluster analysis, Polymorphism, RAPD, Dendrogram.
Abstract: Bananas (Musa spp.) are mostly diploid or triploid with various combinations of genomes inherited from their ancestors. Random amplified polymorphic DNA (RAPD) markers were used for evaluation of genetic diversity of some synthetic Banana Hybrids. A total of 13 random primers were evaluated for banana hybrids based on RAPD polymorphism. Diversity analysis carried out by using RAPD markers n some Banana hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship of the hybrids with their parents. Out of the 13 primers studied, all the primers exhibited scorable markers. However, the maximum number of loci amplified was 18 with primer OPZ 12 and the maximum polymorphism percentage was obtained with the primer OPZ 16.
Location: TE 15 New Biology Building
Literature cited 1: Bhakthavatsalu, C.M. and Sathiamoorthy, S. 1979. Banana clonal situation in India: A resume. Fruits. 11: 34-46. Bhat, K.V., Bhat, S.R. and Chandel, K.P.S. 1992. Survey of isozyme polymorphisms and catalase. Journal of Horticultural Science.67: 501-507.
Literature cited 2: Bhat, K.V. and jarret, R.L and Liu, Z.W. 1994. RFLP characterization on Indian Musa germplasm for clonal identification and classification. Euphytica. 80: 95-103. Bhat, K.V. and Jarret, R.L. 1995. Random amplified polymorphic DNA and genetic diversity in India Musa germplasm. Genetic Resources Crop Evoluation. 42: 107-118. Record No: 60341


ID: 59903
Title: STUDY ON ANTIBACTERIAL ACTIVITY OF HONEY SAMPLES COLLECTED FROM MEDICINAL PLANT SOURCES.
Author: Karuna Pawar, Kedar Rokade and Gajanan Mali.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 755-758 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antibacterial activity, Honey, Agar diffusion method
Abstract: The present investigation describes about the antibacterial activity of three different natural honey samples obtained from different locations of Sangli district. The pH values of these collected honey samples ranges from 4.0 to 4.5. Antibacterial activity of honey at its natural acidic pH as well as by maintaining neutral pH was studied on seven pathogenic bacteria. Marked variations were observed in the antibacterial activity of these honey samples. All the honey samples at natural acidic pH as well as at neutral pH showed a spectrum of antibacterial activity on five different bacterial species such as Salmonella typhi, Shigella dysentriae, Bacillus megaterium, Staphylococcus aureus, and Proteus vulgaris.
Location: TE 15 New Biology Building
Literature cited 1: Al-Walli, N.S., Akmal, M., Al-Walli, F.S., Saloom, K.Y. and Ali, A. 2005. The antimicrobial potential of honey from United Arab Emirates on some microbial isolates. Media Science Monitor. 11: 433-438 Baur, A.W., Kirby, WM.M, Sherris, J.C. and Turck, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical pathology. 36: 493-496.
Literature cited 2: Carmo-Sousa, L., Rose, A.H. and Harison, J.S., 1969. The Yeasts. Academic Press, London. 1: 79-105. Cooper, R.A., Molan, P.C. and Harding, K.G. 1999. Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds. Journal Royal Society of Medicine. 92: 283-285.


ID: 59902
Title: TREATMENT OF PAPER INDUSTRIAL WATER EFFLUENT USING EICHHORNIA CRASSIPES AND PISTIA STRATIOTES.
Author: Suggu Sri Gowri Reddy, A.J.Solomon Raju and Bezawada Mani Kumar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 749-753 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Paper industrial water effluent, Phytoremediation, Eichhornia crassipes and Pistia stratiotes.
Abstract: The rapid development in the industrial sector leading to the drastic changes in the environmental equilibrium and is the prime challenge to scientists as the natural resources are very precious. The paper industrial effluent mainly shows an adverse effect on the water quality parameters like D.O., B.O.D., C.O.D., pH, temperature and conductivity of the surrounding aqua systems at the time of discharge. The abnormal levels of these parameters cause pollution in water bodies and even death of aqua flora and fauna. However, paper industrial effluents are found hazardous and they enter the environment through various channels of waste water discharges. Conventional treatment technologies for the removal of industrial effluents are economical than that of biological methods. Phytoremediation techniques have been found potential to absorb effluents to maximum extent and without possibility of secondary pollution. The prime objective of this paper is to investigate the capability of selected aquatic plant species of Eichhornia and Pistia for the removal of paper effluent toxicity from the contaminated aqueous environment. The changes in the water quality parameters during the treatment of industrial effluent with aquatic plants were verified periodically to optimize the process.
Location: TE 15 New Biology Building
Literature cited 1: APHA-AWWA WPCR, 1998. Standard Methods for Examination of Water Waste Water, 19th edition, APHA, Washington, U.S.A. Chartres, C. and Varma, S. 2010. Out of water. From abundance to scarcity and How to solve the world ' s water problems, FT press (USA)
Literature cited 2: Fang, Y.Y., Yang, X.E., Pu, P.M., Chang, H.Q. and Ding, X.F. 2004. Water eutrophication in Li-Yang Reservoir and ecological remediation counter measures. Journal of Soil and Water Conservation. 18 (6): 183-186. Jackson, M. and Andrew, R.W. 1996. Environmental Science: The Natural Environment And Human Impact. Longman Group Ltd., Harlow, Essex, England.


ID: 59901
Title: STUDIES ON BIODEGRADATION OF "CONGO RED" USING SACCHAROMYCES CEREVISIAE AND LACTOBACILLUS.
Author: Bezawada Manikumar, Pahturi Uma Anil Kumar and Vishnu Unnamatla.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 743-748 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Water pollution, Microbial degradation, Azo compounds, Influencing factors.
Abstract: The discharges of effluent wastewater from the different industries originate adverse effects to the environment and leads to imbalance of bio-systems. Azo compounds are extensively being used as dyes in carpet and textile industries, the water bodies. The conventional treatment technologies for the removal of these dye substances are less economical and cause secondary pollution. Presently, researchers have become increasingly interested to degrade azo compounds by using suitable microbial treatment technologies. The main objective of this paper is to study the capabilities of free, immobilized and co-immobilized cells of Saccharomyces cerevisiae and Lactobacillus sporogenes for the decolourization and degradation of an azo compound ?Congo Red? (CR) from aqueous solutions. The experimental data evidences that the degradation of an azo compound is highly possible with the selected microbial strains and it is more with co-immobilized cells of the selected strains. The influence of various factors viz., pH and initial concentration of biomass on the degradation were verified for the optimization of the process.
Location: TE 15 New Biology Building
Literature cited 1: Alaa, H., Hawari, Catherine N. Mulligan. 2006. Biosorption of lead (II), Cadmium (II), Copper (II) and nickel (II) by an aerobic granular biomass. Bioresource Technology. 97: 692-700. Asad, S., Amoozegar, M.A., Pourbabaee, A.A., Sarbolouki, M.N. and Dastgheib, S.M.M. 2007. Decolorization of textile azo dyes by newly isolated halophilic and halo tolerant bacteria. Bio resource Technology. 98: 2082-2088.
Literature cited 2: Bassett, J., Denney, R.C., Jeffery, G.H. and Mendham, J. 1978. Vogel ' s Textbook of Quantitative Inorganic Analysis, Longman group Limited 4th edn. P. 240-243. Bezawada Mani Kumar and S. Suryanarayana Raju, 2008, Biosorption of heavy metals by free and immobilized cells of Halobacterium cutirubrum, Asian Jr. of Microbiol. Biotech. Env. Sc. 10 (1): 97-104


ID: 59900
Title: COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY OF QUATERNARY AMMONIUM COMPONENT COATED KNITTED FABRICS.
Author: K.M.Pachiyappan, V.R. Sampath and Senthil Kumar
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 739-742 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antimicrobial activity, Knitted structure, Fibre type.
Abstract: The rapid growth I technical textiles and their end-uses has generated many opportunities for the application of innovative finishes. Antimicrobial textiles with improved functionality find a variety of application of innovative finishes. Antimicrobial textiles with improved functionality find a variety of applications such as health and hygiene products and several medicinal applications such as infection control and barrier material. The three-types of knitted fabric samples namely, 100 % tencel, polyster and cotton was used in the present study. The structure of the knitted structures are selected into three different types namely, single jersey, RIB and interlock. All the three knitted samples were finished with antibacterial agent in a similar condition and the efficiency of the antibacterial activity was tested against the standard organisms Escherichia coli and Staphylococcus aureus with the test reference of AATCC 147. The results showed that Rib fabric with tencel structures showed maximum antibacterial activity both qualitatively and quantitatively. In addition to that knitted structures of other cotton, polyester samples are also showed better antimicrobial activity than other knitted structures.
Location: TE 15 New Biology Building
Literature cited 1: Bajaj, P. 2002. Finishing of textile materials. Journal of Applied Polymer Science??? Barani, H. and Peyvandi, S. 2010. Enhanced deep coloring of micro polyester fabric. Materials Science?..
Literature cited 2: Birbir, M. 2003. Tekstil urunlerindeki mikroorganizmalar. Orme Dunyasy, Mayys-Haziran-Temmuz. 74-76. Can, Y., Akaydin, M. and Turhan, Y. 2009. Effect of wrinkle resistance finish on cotton fabric properties. Indian Journal of Fibre and Textiles Research?..


ID: 59899
Title: MICROBIAL ANALYSIS OF SOME FRUIT JUICES AVAILABLE IN THE MARKETS OF KASHMIR VALLEY, INDIA.
Author: Gulzar Ahmad Nayik, Tawheed Amin and Sumanvikas Bhat.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 733-737 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Microbial analysis, Axenic culture, Spread plate method, Fruit juices.
Abstract: The aim of the present study was to assess the microbial quality of fruit juices sold for immediate consumption n the markets of Kashmir valley. Twelve fruit juice samples (3 from each apple, orange, pineapple and mango juices) were procured from different markets and tested for their microbiological quality. Microbial quality was determined by enumerating the total viable count. About 25 % of the samples (orange juice) did not comply with the standards of microbial quality as per the guidelines for microbiological quality of ready to eat foods while as apple, orange and pineapple juices compiled with the standards. The microbial load in orange juice was comparatively higher than that in the apple, pineapple and mango juice which had the microbial load within acceptable limits.
Location: TE 15 New Biology Building
Literature cited 1: Al-Jedah and Robinson, R.K.2001. Nutritional value and microbiological safety of fresh fruit juices sold through retail outlets in Qatar. Pakistan. J Nutr. 1: 79-81. Anali Ramazotti Ferrati, Paula Tavolaro, Maria Teresa, Destro, Maria Landgraf and Bernadette, D.G.M. Franco, 2005. A comparison of ready-to-use systems for evaluating the microbiological quality of acidic as an experimental model. International Microbiology. 8: 49-53.
Literature cited 2: Barro, N., Bello, A.R., S. Aly, Ouattarac, A.T., Ilboudo, A. and Jules Traore, A.S. 2006. Hygienic status on assessment of dishwashing waters, utensils, hands and pieces of money from treetfood processing sites in Ouagadougou (BurkinaFaso). African Journal of Biotechnology. 5 (11): 1107-1112. Bhaskar, J., Usman, M., Smitha, S. and Bhat, G.K. 2004. Bacteriological profile of street foods in Mangalore. Indian J., Med. Microbiol. 22: 197-197.


ID: 59898
Title: ANTIMICROBIAL PROPERTIES OF ROSEMARY (ROSEMARINUS OFFICINALIS L.) ESSENTIAL OIL AGAINST SACCHAROMYCES CEREVISEAE.
Author: Tawheed Amin, Suman Vikas Bhat and Neha Sharma.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 729-732 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Rosemary, Essential oil, Antimicrobial activity, Gas chromatography, Ethnomedicine.
Abstract: Rosemarinus officinalis L. is a perennial shrub and is widely used in ethnomedicine worldwide. The aim of the present study was to assess antimicrobial property of rosemary essential oil against Saccharomyces cerevisiae. The leaves of rosemary plant were obtained from the fields Indian Institute of Integrative Medicine, Sanatanagar, Srinagar, Jammu & Kashmir India while the plants were partially flowered and partially seeded. Essential oil was extracted from rosemary leaves by hydro-distillation method. The study on the antimicrobial activity of rosemary essential oil was carried out by disc diffusion assay. On the performing GC of the essential oil, 18 different compounds were identified, the main constituents being ?-pinene (7.2730%), 1, 8-cineole (13.6826%) and camphor (38.626%). The result showed threat rosemary essential oil can be capable of effectively controlling the growth of S. cereviseae.
Location: TE 15 New Biology Building
Literature cited 1: Abdelaziz, E., Zaira, S. and Benjilali, B. 2000. A study of Moroccan Rosemary oils. J. Essent. Oil Res. 12: 487-495. Amin, T., Gulleria, S.P. and Bhat, S.V. 2012a. A review on the potential applications of rosemary (Rosemarinus officinalis L.) as a natural food additive. Research Journal of Agricultural Sciences (An International Journal). 3 (6): 1165-1169
Literature cited 2: Amin, T., Sharma, N. and Bhat, S.V. 2010b. A general overview on Rosemarinus officinalis L. (Rosemary) as a medicinal plant. Medicinal Plants: International Journal of Phytomedicine and Related Industries. 4 (3): 177-181. Dorman, H.J., Deans, S.G., Noble, R.C. and Surai, P. 1995. In vitro evaluation of plant essential Oils as natural antioxidants. Journal Essential Oil Research. 7: 645-651.


ID: 59897
Title: IN VITRO ANTHELMINTIC ACTIVITY OF JUSTICIA GENDARUSA LINN.
Author: Bonoranjan Phukan, Bibhuti Bhushan Kakoti and Atul Kumar
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 725-728 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Justicia gendarusa, anthelmintic acivity, Pheretima posthuma, Ascardia galli.
Abstract: Ethyl acetate and ethanolic extracts from the leaves of Justicia gendarusa Linn. (Acanthaceae) were investigated for their anthelmintic activity against adult Indian earth worm, Pheretima posthuma and nematode, Ascardia galli. Various concentrations (10, 20, 50 mg/mL) each extract were tested in the bioassay, which involved determination of time of paralysis and time death of the worms. Both the extracts exhibited significant anthelmintic activity at the highest concentration of 50 mg/mL. Piperazine citrate (15 mg/mL) and Albendazole (20 mg/mL) were used as standard references while 0.5 % carboxy methyl cellulose (CMC) in normal saline as control.
Location: TE 15 New Biology Building
Literature cited 1: Ajaiyeoba, E.O., Onocha, P.A. and Olarenwaju, O.T. 2001. In vitro anthelmintic properties of Buchholzia coriacease and Gynandra extract.Pharm. Biol. 39: 217-220. Barik, S.K., Haridasan, K. and Lakadong, N.J. 2007. Medicinal Plant Resources of Meghalaya: Endemism, Threat Status and Consumption Pattern. Envis Forestry Bulletin. 7 (2).
Literature cited 2: Chatterjee, K.D. 1967.Parasitology, Protozoology and Helminthology, 6th ed., Guha Ray Sree Saraswaty Press Ltd, Calcutta. Dash, G.K., Suresh, P., Kar, D.M., Ganapaty, S. and Panda, S.B. 2002. Evaluation of Evolvulus alsinoids Linn. For anthelmintic and antimicrobial activities.J. Nat. Rem. 2: 182-185.


ID: 59896
Title: COMPARATIVE STUDY FOR BIOSYNTHESIS OF SILVER NANOPARTICLES FROM PSEDOMONAS PUTIDA NCIM 2650.
Author: V.Thamilselve and K.V.Radha.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 717-723 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Silver nanoparticles, Silver nitrate, Resistant strain, Nitrate reductase, Ultra violet (uv) spectroscopy, Scanning electron microscopy (SEM).
Abstract: This study reported on the extracellular synthesis of silver nanoparticles from bacterial from bacterial strain Pseudomonas putida NCIM 2650. The silver resistant ability was studied by incubating bacterial strains of Pseudomonas putida NCIM 2650, Bacillus subtilis NCIM 2655 and Lactobacillus acidophilus NCIM 2903 on the Agar plate supplemented with silver nitrate with different concentrations. The tolerant level of silver by those bacteria was found to be 1mM concentration. The silver nanoparticles synthesized from both Pseudomonas putida NCIM 2650 and its silver resistant strain was compared and characterized by UV spectroscopy and SEM. The maximum uv absorbance peak was observed at 420nm. The size morphology of the Pseudomonas putida NCIM 2650 was analysed from SEM Micrograph shows agglomerated and the resistant strain was polydispersed and found to have sizes ranged between 38 and 118nm. The parameters pH and temperature for optimum synthesis was found to be at 6 and 60? C respectively. The optimized silver nanoparticle was subjected to cloud point extraction using non ionic surfactant. The extracted sample of silver nanoparticles showed the enhanced effect of antimicrobial activity against gram positive bacteria, gram negative bacteria and fungi.
Location: TE 15 New Biology Building
Literature cited 1: Amiya Kumar Prusty, 2001.Preparation of silver nanoparticles by microorganism and its application in pharmacy. International Journal of Biomedical and Advance Research, IJBAR Arya Vedpriya, 2010.Living Systems: Eco-Friendly Nanofactories. Digest Journal of Nanomaterials and Biostructures. 5 (1): 9
Literature cited 2: Bijanzadeh, A.R., Vakili, M.R. and Khorad, R. 2012.A study of the Surface Plasmon Absorption band of nanoparticles. International Journal of Physical Sciences. 7 (12): 1943-1948. Ganesh Babu, M.M. and Gunasekaran, P. 2009. Production and structural Characteristics of crystalline silver nanoparticles from Bacillus cereus isolate colloids and surfaces B. Biointerfaces. 74: 191-195.