ID: 53662
Title: Diversification of nitrogen fixing bacterial community using nifH gene as a biomarker in different geographical soils of Western Indian Himalayas
Author: Chhug Singh, Ravindra Soni, Sourabh Jain, Subhadip Roy and Reeta Goel
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 , September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Soil sample, Geographical regions, Diversity, RFLP, NifH
Abstract: Six soil samples (Pantnagar, Chamoli, Almora, Ranichauri, Pithoragarh and Badrinath) belonging to different geographical locations of Western Himalayas in India, were analyzed to diversify the nitrogen fixing bacterial community using nifH gene biomarker, DNA from soil samples were isolated and amplified using nifH gene specific primers. Genomic DNA and PCR amplified products were then individually subjected to restriction digestion with tetra to octacutter enzymes (Alul, Mspl, Bglll, Xbal, Hindlll, Haelll, Alul, Mspl and Pasl. Further, restriction pattern was studied by preparing dendograms on the basis of similarity matrix and compared for the nifH community. It was observed that temperate region soils (Ranichauri and Pithoragarh) were negative for nifH marker while subalpine region (Badrinath) and tarari region soils (Pantnagar) documented similar nifH community. Moreover, the direct genomic DNA restriction analysis indicated that subalpine region soil (Badrinath) was most diversified.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53661
Title: Identification of bacterial strains from tannery effluent and reduction of hexavalent chromium
Author: Soha Farag and Sahar Zaki
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Chromate-resistant bacteria, Cr(VI) reduction, Minimal inhibitory concentration, Immobilization
Abstract: Four chromium-resistant bacteria were isolated from tannery effluent collected from Burgelarab, Alexandria, Egypt. These isolates displayed different dgrees of chromate reduction under aerobic conditions. Based on 16S rDNA gene sequence analysis, two of them (S3 and S4) were identified as Acinetobacter, and Pseudomonas, respectively. The minimum inhibitory concentration for Acinetobacter sp. strain S3 was 160 mg l-1, while it was 200 mg l-1for Pseudomonas sp.strain S4. However, stain S4 was able to reduce a wide range of Cr(VI) concentrations for 20 to 200 mg l-1, while, it was reducing 64.4% of Cr (VI) at 160 mg l-1 within 72 hr. Immobilization experiments demonstrated that strain S4 in calcium alginate gel matrix was more effective than the using of free cells in chromium reduction.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53660
Title: Effects of calcium nitrate and humic acid on pepper seedling growth under saline condition
Author: Fusun Gulser, Ferit Sonmez and Sibel Boysan
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Pepper seedling, Salt stress, Humic acid, Calcium nitrate, Growth
Abstract: This study was carried out in order to determine effects of calcium nitrate and humic acid applications on pepper seedling growth under saline condition. A mixture of soil: sand (1:1) was used as a plant growing media including 90 mg kg-1P2O5, 180 mg kg-`1K2O and 250 mg kg-1N. Four different doses of humic acid (0, 1000, 2000 and 4000 mg kg-1) and calcium nitrate (0, 50, 100 and 150 mg kg-1) were applied into the growing media. Before sowing Demre variety pepper seeds, 60 m mol NaCl was added into 300 cm3 soil:sand mixture in each pot. Applications of humic acid and calcium nitrate significantly affected pepper seedling growth. 1000 and 2000 mg kg-1humic acid and 50 mg kg-1 calcium nitrate applications increased fresh and dry leaf weight, fresh and dry root weight, stem diameter, root length and shoot length. The highest rates of humic acid (4000 mg kg-1) and calcium nitrate (100 and 150 mg kg-1) decreased these criteria of pepper seedling under the saline soil condition.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53659
Title: Antibacterial activity of house fly-maggot extracts against MRSA (Methicillin-resistant Staphylococcus aureus) and VRE (Vancomycin-resistant enterococci)
Author: Sang O Park, Jae Hong Shin, Won Keun Choi, Byung Sung Park, Jin Seok Oh and Aera Jang
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Maggot extracts, MRSA, Antibacterial activity, Minimum inhibitory concentration
Abstract: The principal objective of this study was to determine the in vitro antibacterial activity of the water -soluble protein enzymatic hydrolysates and the ethanol (EtOH)- extracted fraction obtained from fly-maggots (Musca domestica L.) against MRSA (Methicillin-resistant Staphylococcus aureus) strains adn VRE (Vancomycin - resistant enterococci) 5117 strain. The water soluble protein enzymatic hydrolysates were prepared via 4 or 8 hr of incubation after mixing with the water-soluble protein extracted from the fly-maggots plus thermolysin. The EtOH-extracts A was prepared by homogenizing after mixing with the fly-maggots plus pure EtOH at room temperature. The EtOH- extracts B and C was obtained via filtering after stationing for 24 hr at 4-200C using the EtOH-extracts A, respectively. The growth inhibition curves for MRSA strain 3595 and VRE strain 5117 in the water-soluble protein enyzmatic hydrolysates were increased and evidenced concentration-dependent inhibition in the 8-hr hydrolysate as compared with the 4-hr hydrolysate (p<0.05). The growth inhibition curves for MRSA and VRE strains in the EtOH-extracted fraction obtained from the fly-maggots were high in EtOH-extract C as compared with EtOH-extracts A and B (p<0.05). The minimum inhibitory concentrations (MIC) in the EtOH-extracts C. In which the growth inhibition of MRSA and VRE strains was increased, were determined to be 40, 50, 50, 60, 40 and 60 ?gml-1 in MRSA strains 3598, 3595. 3601. 3589, 3597 and 3595 , respectively. While the butanol fraction obtained from EtOH extract C evidenced profound antibacterial activity against the MRSA and VRE strains, the antibacterial activity of the hexane, ethyl acetate, and water layers could not be adequately confirmed.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53658
Title: Path coefficient analysis between seed cotton yield nad some characters in cotton (Gossypium hirsutum L.)
Author: Remzi Ekinci, Sema Basbag and Oktay Gencer
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Yield, Path coefficient, Correlation, Cotton
Abstract: This study was carried out to determine direct and indirect effects of some characters (single leaf area, leaf SPAD value, number of nods, number of sympodial branches, number of monopodia branches, single boll weight, number of bolls) on seed cotton yield. The experiment was carried out with four cotton varieties, two of which okra -leaf (Adana 98, Siokra 1/4) and two of which normal-leaf (Teks and Stoneville 453) cotton (Gossypium hirsutum L.) and was conducted the randomized block design with 4 replications. In order to increase seed cotton yield in the studies of breeding program and selection orderly leaf SPAD value, number of sympodial branches, single boll weight, number of monopodial branches and number of bolls characters determined that must be high. Results showed that in order to increase seed cotton yield in the studies of breeding program and selection the highness of leaf SPAD value, number of sympodial branches, single boll weight, number of monopodial branches and number of bolls characters should be considered important.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53657
Title: Effects of housing systems on physiological and energetic parameters in comisana ewes
Author: P. Pennisi, L. Biondi, S.Casella and G.Piccione
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Housing systems, Physiological parameters, Energetic parameters, Ewes
Abstract: The pattern of some physiological parameters (rectal temperature, respiratory rate and heart rate) and some energetic parameters (glucose, triglycerides, total cholesterol and total lipid) has been investigated with the purpose to assess the influence of shearing on thermoregulation. Thirty-six Comisana ewes were divided into two groups of 18 subjects and were allowed to two different housing systems: animals of group A were housed in an open -front barn with a paddock and animals of group B to a paddock equipped with shading net. After a period of adaptation to the experimental conditions, individual rectal temperature, respiratory and heart rates were measured. The serum concentrations of glucose, triglycerides, total cholesterol and total lipid were also assessed. All parameters were recorded, with an average interval of eleven days during 90 days, from July 15th to October 11. The statistical analysis, the ANOVA followed by the Bonferroni ' s test, showed a significant effect of the time for respiratory rate, heart rate and glucose and it showed that during experimental period the type of housing system influenced glucose, triglycerides, total cholesterol and total lipid. These results confirm that different micro-environmental conditions can influence thermoregulatory mechanisms with effects on the productivity and on the welfare of ewes.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53656
Title: Protective effect of Kombucha mushroom (KM) tea on chromosomal aberrations induced by gamma radiation in human peripheral lymphocytes in-vitro
Author: Kultigin Cavusoglu and Perihan Guler
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Gamma radiation, In-vitro chromosome aberration, Kombucha mushroom tea, Radioprotective effect
Abstract: The aim of this study was to investigate the potential radioprotective effect of kombucha mushroom tea (KM-tea) on gamma radiation (?)- induced chromosomal aberrations (CAs) in human peripheral blood lymphocytes in vitro. For this purpose, we used in vitro dose-effect relationship, and correlated these data with statistical parameters. CAs were classified into six major types as break, dicentric, acentric, fragment, gap and ring. Mitotic index (MI) and the numbers of aberrant metaphases (AMN) were also calculated for each donor. Six groups of the lymphocytes were prepared by in vitro culture according to the standard protocol. Group I (control) did not receive any ?-radiation or KM-tea, Group II (positive control) was treated with 1000? l dose of KM-tea alone, Group III was treated with 5 Gy dose of ?-radiation alone, Group IV was treated with 250 ? l dose of KM-tea before irradiation, Group V was treated with 500 ? l KM-tea before irradiation, Group VI was treated with 1000 ? l KM-tea before irradiation. The results indicated that all KM-tea supplemented lymphocytes had lower frequency of CAs than in the group treated with ?-radiation alone (p<0.05). It was seen that KM-tea had a protective effect against CAs particularly at 500 and 1000 ? l doses. Besides, MI values increased and AMN decreased after application of KM-tea in a dose/dependent manner. In vitro results showed that KM-tea supplementation may decrease the frequency of CAs and its radioprotective action against ionizing radiation is dose-dependent.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53655
Title: Characterization of the cell membrane during cancer transformation
Author: Barbara Szachowicz-Petelska, Izabela Dobrzynska, Stanislaw Sulkowski nad Zbigniew Figaszewski
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Phospholipids, Free fatty acids, HPLC, Electric charge, Electrophoresis, Colorectal cancer cells
Abstract: The electric properties of the plasma membrane is an indicator of cell condition. The simple, and highly effective, normal-phase (NP) and reversed-phase (RP) high-performance liquid chromatography (HPLC) methods assess phospholipid and free unsaturated fatty acid content, respectively. Herein we focus on changes in phospholipid content [phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanoloamine (PE), phosphatidylcholine (PC)] and free unsaturated fatty acid content [arachidonic acid (AA), linoleic acid (LA), ?-linolenic acid (ALA), palmitoleic acid (PA)] in the plasma membrane of non-metastatic colorectal cancer cells (pT3 stage, G2 grade). Surface charge density of normal and tumor large intestine tissue was measured by electrophoresis. The surface charge density as a function of pH, acidic (CTA) and basic (CTB) functional group concentrations and their average association constants with hydrogen (KAH) or hydroxyl (KBOH) ions were evaluated. Cancer transformation was accompanied by an increase in total phospholipids as well as and increase inCTA, CTB and KBOH whereas the content of free fatty acids and KAH decreased compared with unchanged tumor cells.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53654
Title: Alterations in serum biochemical parameters of patients with lung cancer exposed to radiotherapy
Author: Kultigin Cavusoglu, Sukran Cakir Arica and Cengiz Kurtaman
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Lung cancer, Enzyme parameters, Gamma radiation, Radiotherapy, Serum elements
Abstract: In this study, the alterations in serum biochemical parameters of patients with lung cancer exposed to radiotherapy was investigated. For this aim, the levels of serum gamma glutamyltransferase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin (ALB), bilurubin (BLB), copper (Cu), Sodium (Na) and potassium (K) were evaluated before and after radiotherapy. Serum enzyme, protein, Na and K levels were determined using an autoanalyzer. Serum Cu analysis was made with Atomic Absorbtion Spectrophotometer (AAS). Although we found significant increases in levels of GGT, BLB, Cu and K in patients, levels of AST, ALT, ALB and Na in patients showed significant decreases. The levels of serum AST and ALT fairly decreased after radiotherapy. The level of GGT in patients was significant higher than that in the controls before radiotherapy. However, GGT level showed again a distinctly decrease after radiotherapy. There was an inverse relationship among serum BLB, Cu and ALB values. Besides, serum Na levels showed significantly decrease in patients at the end of radiotherapy treatment compared to the controls and before radiotherapy, and K levels increased significantly following radiotherapy. In conclusion, the selected serum parameters are very sensitive and useful biomarkers for the study of the effects of radiotherapy.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53653
Title: Plankton diversity and dynamics in a polluted eutrophic lake, Ranchi
Author: B. Mukherjee, M. Nivedita and D. Mukherjee
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Blooms, Cluster analysis, Eutrophic, Importance value index, Species diversity, Saprobity, Ranchi lake
Abstract: The species diversity of a cultural eutrophic lake at Ranchi was studied in relation to external variables (forcing functions) and internal or state variables. The lake receives daily detergent in puts in the form of washings of a variety of objects. A model was constructed for the estimation of detergent inputs from the increase in the phosphate concentration, and from changes in the concentration of inorganic carbon. Nutrients such as inorganic carbon, nitrates, phosphates, sulphates were found to be high in constrast to natural unpolluted systems. The DOM, COD and BOD were also found to be high suggesting organic pollution of the system with an organic carbon load of 5.4 m molesl-1. the growth and development of the plankton constituents was studied in this regime. The natural planktonic rhythm was found to be modified by the polluted condition existing in the lake. The phytoplankton exhibited four peaks in March, May, August, and November while, the zooplankton showed three peaks in February, July and October. The abundance of zooplankton during the annual cycle oscillated with that of the phytoplankton. There was much more evenness in the zooplankton population in comparison to the phytoplankton. Analysis of both, the zooplankton as well as the phytoplankton population was done using the Bray-Curtis dissimilarity index, importance value index and Shannon-Weaver diversity index. The importance value index was found to provide a better evaluation of the plankton community than the diversity index. The phytoplankton population showed no correlation with nutrient availability as indicated by the correlation-regression analysis and the planktonic rhythm was not in tune with normal unpolluted conditions. The lake was classified as meso-polysaprobic using biological and chemical indices (Pantle and Buck index:3.5, BOD:60; DOM:9.3 and COD:130).
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53652
Title: Impact of sewage disposal on a nematode community of a tropical sandy beach
Author: Mandar Nanajkar and Baban Ingole
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Free-living nematodes, Daptonema sp, Sandy beech, Bacterial feeding, Pathogen, Sewage pollution, Bio-remediation, West Coast
Abstract: Free-living marine nematodes from a intertidal sandy beach from Goa near the Panjim city, central west coast of India was investigated along a gradient of sewage pollution. High nematode diversity (11 species) nad abundance was observed near the sewage discharge point, which decreased gradually away from the discharge site. The salinity and dissolved oxygen of the estuarine water increased from the discharge point whereas reverse trend was observed for the sedimentary organic carbon. The total nematode densities indicated three-fold increase (from 523 to 1769 ind. 10 cm2) in 25 yr with a constrasting gradient of nematode abundance, spatially from the source point of sewage discharge. Daptonema sp is known to be a good indicator of stressed and polluted habitats and was observed to be the most dominant species at the study site. Being exposed to the domestic sewage, the area also has high counts of pathogenic bacteria (e.g. E. coli and other coliform types). Daptonema sp are known to consume bacteria and presence of high bacterial biomass due to nutrient enrichment from the discharged sewage enhanced their abundance. Thus, the increasing nematode densities specifically like Daptonema sp at organically polluted sites can be of immense aid to reduce pathogenicity and can potentially be applied in pollution management and act as agents of natural bio-remediation.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53651
Title: Elevated CO2 influences photosynthetic characteristics of Avena sativa L cultivars
Author: R.K.Bhatt, M.J.Baig and H.S.Tiwari
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Biomass production, Oat, OTC, Specific leaf mass, Photosynthesis, Stomatal conductance
Abstract: The impact of elevated CO2 concentration on the growth, photosynthsis and biomass production was investigated in three oat (Avena sativa L) cultivars viz. Kent. JHO-822 and JHO-851 by growing under three environmental conditions i.e. elevated CO2 at 600 + 50 ? mol mol-1(C600), OTC with ambient CO2 (COTC) and under open field condition (Ca). Plant height and leaf area increased in the elevated CO2 grown plants. JHO-822 attained maximum height under C600 followed by Kent and JHO-851. The specific leaf mass (SLM) and specific leaf area (SLA) were also influenced significantly when the plants were grown under C600. Kent showed highest SLM under C600 corresponding lower value of SLA. The accumulation of soluble protein in the oat leaves decreased under C600 except JHO-822 where marginal increase in soluble protein was recorded under C600. JHO-822 showed an increase in Chlorophyll a, b and total in C600 over Ca, whereas other two cultivars did not follow any specific trend in the pigment accumulation. Our results confirmed that the net phosynthetic rate (PN) increased by 37% in Kent followed by JHO 822 under elevated CO2 over the control. This strong association PN of with gs was evidenced by a positive significant correlation (r=0.885**). A clear stimulatory effect at elevated CO2 was detected in all the cultivars in term of green and dry matter production than at ambient CO2 and COTC. A large increase in PN in the present investigation was accompanied by relatively small decrease in gs , which limits the water loss through transpiration rate. The elevated CO2 induced changes in gs and reduction in transpiration.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53650
Title: Effect of distillery spent wash on cytomorphological behaviour of sugarcane settlings
Author: Sangeeta Srivastava and Radha Jain
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Chromosome aberrations, Crytotoxicity, Distillery waste, Heavy metal, Pollution, Saccharum, Sugarcane
Abstract: Characteristics of crude and digested spent wash (distillery waste), and effect of their application on cyto-morphological attributes of settlings of eleven genotypes of sugarcane (Saccharum species hybrids) wer studied. High concentrations of K, P, S, Fe, Mn, Zn and Cu contents and heavy metals were present in crude spent wash as compared to the digested one. Root meristem assay of settlings grown on crude and digested spent wash showed a detrimental effect on mitotic efficiency and also induction of de novo chromosomal aberrations viz. clump formation, chromosome stickiness, laggards and micronuclei formation etc. higher number of chromosomal abnormalities as compared to those of control conditions. Mitotic index of root-meristems of different genotypes showed a decline of 62.65 to 100% in crude spent wash treatment and 36.94 to 90.33% decline in digested spent wash treatments as compared to the control. However, an improvement to the extent of 27.73% was observed in the mitotic activity of root-meristems treated with diluted spent wash (1: 5 v/v with water). Inhibitory effects of digested and crude spent wash were also visible on bud sprouting and settling height, but 1:5 aqueous dilution of spent wash stimulated the early growth attributes. Such beneficial effects of diluted spent wash were observed in most of the sugarcane genotypes.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53649
Title: Studies on morphological and genetical similarities of Medicago murex and M. doliata to M. scutellata
Author: Amaresh Chandra
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Hypera postica, Isozyme, Medicago species, SSR, Weevil resistance
Abstract: Lucerne (M. sativa L., 2n = 4x =32) is susceptible to weevil (Hypera postica Gyll) insect, hence incorporation of desirable gene (s) from M.scutellata (2n =30) is an important researchable issue. Incompatibility due to incongruous chromosomal arrangements in these two species necessitated the identification of closer species to M. scutellata (possibly progenitors). After screening 197 accessions two species necessitated the identification of closer species to M. scutellata (possibly progenitors). After screening 197 accessions comprising 50 Medicago species, M.murex (2n = 2x = 14) and M. doliata (2n =2x= 16) have been identified as morphologically similar having compatible ploidy and genetically closer to M. scutellata as observed with 17 simple sequence repeats (SSR) and 8 enzymes based isozyme markers. The identified accessions namely Il-04-223 and IL-04-151 of M.doliata and M.murex respectively showing low levels (<5%) of weevil infestation can be contemplated with diploid M. sativa (2n =2x=16) to generate weevil resistant lines.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 53648
Title: Calcium interaction with salinity-induced effects on growth and metabolism of soybean (Glycine max L.) cultivars
Author: A. Arshi, A. Ahmad, I.M. Aref and M. Iqbal
Editor: Dr. R.C.Dalela
Year: 2010
Publisher: Triveni Enterprises, Vol 31, No 5 (Supplement), September 2010
Source: Centre for Ecological Sciences
Reference: None
Subject: Journal of Environmental Biology
Keywords: Antioxidant enzymes, Biomass accumulation, Glycine max, Ion accumulation, Salinity stress
Abstract: In the present work, hydroponic culture of JS-335 and Bragg cultivars of soybean (Glycine max) were raised to analyze changes in growth, reactive oxygen metabolism in terms of H2O2 content, lipid peroxidation (TBARS), free radical quenching systems (non-enzymatic and enzymatic antioxidants) and ion accumulation in different plant parts under NaCl and CaCl2 stress. Fifteen -day-old seedlings were treated with solutions of 25 mM (T1), 50 mM (T2) and 100 mM(T3) NaCl alone and in combination of 10 mM CaCl2 i.e, 25 mM + 10 mM (T4), 50 mM + 10 mM (T5) and 100 mM + 10 mM (T6). Observations recorded at 30 days after sowing displayed significant decreases in plant biomass, leaf water potential, leaf area, chlorophyll content and the contents of glutathione (GSH) and ascorbate (AsC) on application of NaCl alone. However, H2O2 content and lipid peroxidation (TBARS) in leaves were enhanced, consequently invoking the activities of SOD, APX, GR and CAT. Application of NaCl + CaCl2 alleviated adverse effects of NaCl stress. The Na+ and Cl- contents in different plant parts increased with NaCl as well as with NaCl + CaCl2 treatments. The maximum accumulation occurred in roots, followed by the stem and the leaves. The K+ and Ca2+ contents decreased under NaCl stress; but NaCl + CaCl2 treatment reduced the extent of decrease caused by NaCl. Thus, calcium ameliorated the deleterious effects of NaCl stress and stimulated plant metabolism and growth.
Location: 241
Literature cited 1: None
Literature cited 2: None