ID: 59107
Title: Biosynthesis of Alkaline Phosphatase by Non-Symbiotic Diazotrophs.
Author: Soma Pal Saha, Anindita Bhattacharya, Sagnika Ghosh, Subhasis Ghosh, Sourav Ghosh, Huma Shaz, Swapan Bhattacharya.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 411-418, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Alkaline phosphatase, Azotobacter, Diazotrophs, Phosphate solubilizing bacteria.
Abstract: A large number of diazotrophs organisms have the ability to solubilize the immobilized phosphate. These microorganisms can be used as efficient biofertilizers to supply sufficient amount of nitrogen and phosphorous to the plants. Following enrichment and dilution plating on nitrogen-free Stockdale tricalcium phosphate medium such organisms were isolated from the soil. Among a subsample of 15 isolates, 14 were gram negative and 80% of them were coccoid. An efficient phosphate solubilizing isolate, strain UT01 had been presumptively identified as Azotobacter sp. The cultural condition for optimum biosynthesis of phosphatase by this strain under laboratory conditions was determined. In the said medium with 0.5 % (w/v) phosphate, the strain secreted 16.5 units of enzyme/ml of culture. Medium with lower concentration of phosphate 0.3% (w/v), pH 8.0 and incubation temperature 35 C, appeared as growth promoting and this condition accelerated enzyme secretion by 43%. Furthermore, the strain synthesized phosphatase enzyme, which showed almost same activity at pH ranging from 8.0 to 9.0.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59106
Title: Polymerase Chain Reaction for the Confirmation of Isolated Listeria SPP. from Food Contact Surfaces in the Open Markets in Khonkaen, Thailand.
Author: P Pasuwan, R R Beumer, H Den Besten, B Leenanon.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 401-410, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Food contact surfaces, Listeria spp, L monocytogenes, CAMP test, Polymerase Chain Reaction.
Abstract: Swab samples (280) were taken from different kinds of food contact surfaces including stainless steel, tile, polythylene and wooden cutting board from five open markets in Khonkaen, Thailand. Listeria spp, was found from food contact surfaces in three open markets. Most positive samples were isolated from surfaces of stainless steel, wooden cutting board, ceramic tile and polythylene. All suspected colonies were identified using biological tests such as Gram stain, mobility catalase, and CAMP Tests. The weak hemolytic colonies of the CAMP reaction on 5% sheep blood agar defined that 13 out of 70 colonies were identified as L.monocytogenes. Listeria identification tests were performed by 10 Listeria API strips. All weak hemolytic strains were confirmed by PCR. PCR products amplified with 16sRNA primers were sent for sequencing. Results showed that all isolated strains amplified with the general Listeria primer were confirmed as Listeria spp. However, the PCR products bands which were amplified specifically for L. monocytogenes were missing on 1.5% Agarose gel. The PCT results implied that all isolated strains were not L. monocytogenes. Blasting results form EMBL-EBI and Ezbiocloud databases reported that all isolated strains with weak hemolytic results were L, innocua.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59105
Title: Production of Ethanol with Some Starchy Substrates by some Yeast Strains.
Author: B S Anuradha, S Jeevan Chandra, S Ram Reddy.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 393-400, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Yeast, Ethanol, Starches, Amylases, Reducing sugars.
Abstract: Investigation on possible use of different starches viz., potato starch, rice starch and corn starch as raw materials for ethanol production with seventeen strains of yeasts revealed that potato starch followed by cassava starch was found to be suitable, because of their easy digestibility by the two commercial enzymes, viz., Biotempase and Amylo 300L employed in the present investigations. Among the different ratios of enzymes investigated, a ratio of 3:7 of the two enzymes was found to yield better results in terms of both release of utilizable sugars and alcohol production. Interestingly, some strains were found to hydrolyze the raw starches, though in small quantities, even in the absensce of externally supplied enzymes. In majority of the cases, good positive correlation between the released reducing sugars and alcohol produced was observed.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59104
Title: Biodegradation of Pulp Waste Effluent from T N P L by Bacillus SP.
Author: V Kamalaveni, V Prabhakaran.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 385-388, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Pulp, Total dissolved solids, Bacillus sp.
Abstract: The present investigation is an attempt to address the concern for pulp, waste effluents water of T.N.P.L karur, by using microorganism Bacillus subtilis sp, to assess the biodegradation of the effluents. The pulp waste effluents were collected and their physic chemical; characteristic were studied. Besides, the optimum temperatures for the growth of microorganism were found to be 37 C and optimum pH was found to be 6.5-7. The level of B.O.D and C.O.D was found to be reduced during 25 days of exposure. The present study suggested that using sporulating organisms like Bacillus. The biodegradation of paper mill effluents can be achieved as an eco-friendly novel alternative compared to traditional chemical treatment.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59103
Title: Phytochemical and Antimicrobial Activity of Dolichandrone Falcata Stem on some Drug Resistance Bacterial Species.
Author: A S Bagwan, M E Kishore.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 379-384, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Dolichandrone falcata, Medshingi, Antimicrobial activity, Phytochemical properties, Zone of inhibition.
Abstract: The present study aims to evaluate the possibility for the presence of novel bio-active compounds against pathogenic bacteria, as most of the pathogens develop drug resistance against commonly used antibiotics. To determine antibacterial activity, stem extract of Dolichandrone falcata extract shows very promising result. The aqueous, acetone and ethanol extracts of plant stem was prepared and evaluated for its phytochemical properties and antimicrobial activities. The phytochemical analysis of the preparation revealed the presence of some secondary metabolites which includes: tannins, flavanoids, glycosides, steroids, saponins and alkaloids. The In vitro antimicrobial activity of the crude aqueous, acetone and ethanol extract of the Dolichandrone falcata were investigated. The extract showed antimicrobial activities against the test organisms with different zones of inhibition. The minimum inhibitory concentration (MIC) of the acetone extract was between 10-20 mg/mL. The ability of the crude extracts of Dolichandrone falcata to inhibit the growth of microbes is an indication of its antimicrobial potential, which may be employed in management of microbial infections.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59102
Title: Effect of Different Growth Regulators on In Vitro Nodal Explant of Kinnow Mandarin in Northern Rajasthan, India.
Author: Chandra Gurnani, Vikram Kumar, Shinam Mukhija, Poonam Narula, Abni Sharma.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 375-378, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Auxin, Cytokinin, In vitro, Kinnow mandarin, Plantlet.
Abstract: Studies were initiated on the role of various growth regulators in regeneration process of Kinnow mandarin. In vitro nodal explants were cultured on MS media supplemented with different concentration of cytokinin (BAP, Kinetin), auxin (NAA, IAA, IBA) and combination of cytokinin with auxin to produce plantlets. Best shoot multiplication obtained on MS medium supplemented with BAP (1.5 mg/L) and NAA (0.5 mg/L). Transfer of shoot regenerated plantlet inMS media supplemented with NAA and IBA for root proliferation. Highest percentage of roots obtained on MS media supplemented with NAA (1.5mg/L).
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59101
Title: Fermentation of Municipal Solid Wastes by Bacterial Isolates for Production of Raw Protein Degrading Proteases.
Author: Abul Kalam Azad, Akhikun, Nahar, M D Mahbub Hasan, Kamrul Islam, M D Faisal Azim, M D Saddam Hossain, Mohammad Rejaur Rahman, Ravikant Ojha, G M Shamim Mahmud, Rafidin Kayes.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 365-374, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Bacterial isolates, Degradation, Fermentation, Municipal solid wastes, Protease.
Abstract: To produce extracellular bacterial protease using municipal solid wastes (MSW), we isolated three proteolytic bacterial isolates from MSW and identified them as Bacillus sp., Pseudomonas sp and Serratia marcescens. Optimum temperature and pH for maximum protease production by Bacillus and Pseudomonas isolates were 37? C and 7.0, respectively and those by S.marcescens were 30? C and 8.0, correspondingly. With proteinous and cellulosic MSW, cultivation period for optimum protease production by Bacillus and Pseudomonas and S. marcescens was 48 h, however, with a basal media, it for Bacillus, Pseudomonas and S. marcescens was 48, 42 and 24 h, respectively. Crude proteases from the three isolates were stable at pH 6.0-8.0 and at temperatures 25-45?C and were either cysteine and/or serine types. Proteases from Bacillus, Pseudomonas and S. marcescens degraded about 20, 45 and 50% dry weight of the chicken meat, respectively after 48 h of treatment.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59100
Title: Influence of Calcium Phosphate Nano Gel Fertilizer Composites on Germination of Crop plants.
Author: R Umarani, R Mala.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 327-331, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: SEM, Calcium phosphate nano gel fertilizer composite, Mean germination rate and Vigour index.
Abstract: Agriculture is the backbone of Indian economy. At present the contribution of agriculture to Indian economy is steadily decreasing due to rapid Industrialization. Population explosion and its consequence of increased demand for food urge Science and Technology to devise an alternative strategy to increase agricultural productivity with limited availability of land and water resources. In the present study, the Influence of Calcium Phosphate Nano Gel Fertilizer Composites (CAPNGFC), urea (10mg/L), diammonium phosphate (20mg/L) and dipotassium hydrogen phosphate(20mg/L) Fertilizer Composites (FC) and Calcium Phosphate Nano Gel (CAPNG) on germination of four plants were evaluated. CAPNG was synthesized and the size and the morphology was determined by SEM. 1 in 5 dilution, 1 in 10, 1 in 15 and 1 in 20 dilutions of CAPNGFC was also used to assess their influence on germination of Oryza sativa, Arachis hypogea, Abelmoschus esculentus and Amaranthus spinosus. Seed germination was 85%, 88% and 90% respectively in O. sativa, A.hypogea and A.spinosus etc. in control. diluted calcium phosphate nanogel has no stimulatory role.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59099
Title: Study of Metallo Beta Lactamase in Meropenem Resistant Clinical Isolates in a Teritary Care Hospital.
Author: Vivek Agwan, Ritu Kansal, Ashish Asthana, Molly Madan.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 309-312, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Carbapenemase, EDTA disk synergy test, Gram negative bacilli, Metallo-B-lactamase.
Abstract: This study was undertaken to detect metallo-?-lactamase (MBL) in meropenem resistant clinical isolates of Gram negative bacteria. A total of 100 clinical isolates of meropenem resistant Gram negative bacteria, were selected for this study. All the isolates were screened for MBL production, by EDTA disk synergy (EDS) test, using both imipenem and meropenem seperately. Among the 100 meropenem resistant clinical isolates 29 were found to be MBL producers. MBL production was detected in 19 out of 38 (50.0%) Pseudomonas species, 3 out of 46 (6.5%) Acinetobacter species, 5 out of 9 (55.5%) Klebisiella species, 1 out of 6 (16.6%) Escherichia coli and 1 (100.0%) Citrobacter species. MBL production is an important mechanism of carbapenem resistance and its prevalence amongst Gram negative bacteria is being increasing reported worldwide, not only in Pseudomonas species but also amongst other Gram negtive bacteria. Detection of MBL producing gram negative bacilli is crucial for optimal treatment of patients and also to control the spread of resistance. EDS test is a sensitive test and is better than or comparable to other phenotypic tests for MBL detection. In our study EDS test using imipenem detected 29 and using meropenem detected 23 MBL producers. The difference between MBL producers detected by EDS test using imipenem and meropenem was 6 (P=0.970), which is not significant. Being inexpensive and easy to perform, EDS test using imipenem can become part of mainstream clinical laboratory testing for MBL.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59098
Title: Population Dynamics and Diversity of Soil Microbes in some areas of Jalpaiguri District of West Bengal, India.
Author: Soumendranath Chatterjee, Kuntal Kanti Chattoraj, Puja Banerjee, Mainak Kumar De, Arjit Majumdar.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 287-290, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Jalpaiguri, Soil microbial diversity, Physico-chemical properties of soil.
Abstract: Six soil samples were collected from four different locations with different micro-environment namely, Batabari farm side area, Batabari village area (two samples), Murti river side (two samples) and Mahakal Mandir forest area of Jalpaiguri District, West Bengal . Topography, land slope, vegetation coverage, water logging condition and anthropogenic influences were considered as basis of the sample selection. The population dynamics, diversity of different groups of microrganisms were determined. Soil chemical and physical characteristic features were also analyzed. Aerobic heterotrophic bacteria, Nitrifying bacteria, Gram(-) bacteria, Phosphate solubilising bacteria, Nitrogen fixing bacteria and Fungi population were quite high in Mahakal Mandir forest part with high percentage of organic carbon. These popultion were quite high in Mahakal Mandir forest part with high percentage of organic carbon. These populations were somewhat low in other two areas of Batabari village area and Batabari farm side area due to human interference. Murti river bank areas showed a different picture where all bacterial groups were very low organic carbon, leaching activity and low water holding capacity in coarse grained soil.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59097
Title: Assessment of Water Quality of Polluted Lake using Bacterial Indicators.
Author: G K Amte, Tejali A Gore.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 273-278, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Fresh water bodies, MPN, coliforms.
Abstract: Most of the water borne pathogenes gain entry into the water bodies via sewage contamination which leads to health hazards. The present study was conducted to evaluate quality of water collected from tropical Lake system. Water samples were monitored between 7.00 am. to 8.00 am. for the presence of coliforms varying levels of bacteriological contamination were recorded. Results of this study showed that the water quality has deteriorated and its is important to provide sanitary monitoring program for the water.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59096
Title: Screening of Potential Lignin Degrading Fungi from the Litter of Mangifera INDICA.
Author: P A Geethanjali, Shradha J R.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 255-257, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Degradation, Screening, Lignin, Lignolytic activity , Litter.
Abstract: Litter is the surface layer of the forest floor consisting of freshly fallen leaves, needles, twigs, stems, bark and fruits. Litter biota varies with depth and with stage of decay. Litter contain about 20-50% lignin. Because of the size and complexity of lignin, its decomposition rate is slow. Fungi especially basidiomycetes are generally recognized as the major group responsible for lignin degradation. The lignolytic enzymes are being used in food, textile, paper industries and in the degradation of lignin rich agro-waste. In the present work lignin degrading fungi were isolated from the litter of Mangifera indica. About 58% of the total fungi isolated were found to be potential lignin degraders. The isolates were tested for enzyme activity and those showing maximum activity were tested for their efficiency to degrade areca and coffee husk. The Chaetomium sp, Penicillium sp, Aspergillus sp and Trichoderma sp were found to degrade areca and coffee husk effectively.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59095
Title: Evaluation of Antifungal Property of Pseudomonas Fluorescens as a Biopesticide against Phytopathogenic Fungi.
Author: C J Chandekar, S Bhaje.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 249-253, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Biopesticides, Pseudomonas fluorescens, agrowaste.
Abstract: Biopesticides include naturally occuring substances that control pests and are considered eco-friendly and easy to use. In present study, the antifungal activity of Pseudomonas fluorescens was assessed. Pseudomonas fluorescens was cultivated using banana peel out, watermelon skin or agro-waste showed that the strain was capable of meeting its components required for growth. Pseudomonas fluorescens proved to be active against some plant pathogenic fungi such as Curvularia Lunata, Alternaria Cajani, Helminthosporium etc. Being able to inhibit the growth of phyto-pathogenic fungi Pseudomonas may potentially be used as a bio-pesticide. So it was concluded during the study that Pseudomonas can be used as a bio-pesticide. So it was concluded during the study that Pseudomonas can be used as a Bio-pesticide against plant pathogenic fungi and it can also be grown efficiently on agro-waste.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59094
Title: Performance Evaluation of Flexible Pavements on Expansive Soils.
Author: C S Bhagvan Raju, M Anjan Kumar, G V R Prasada Raju.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 241-248, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Expansive soil, flexible pavement.
Abstract: As part of infrastructure development huge investment is being made on expansion of National highways and important roads across the country. For improvement of Highways two types of Pavements commonly used in India viz., Flexible Pavements (Bituminous) and Rigid pavements (Concrete). Flexible pavements are widely used in this country from the considerations of economy. The performance of Flexible pavements depends largely on properties of original ground on which the pavements rests, the quality of materials used in the construction of various layers of pavements and quality assurance as per relevant specifications. In general any road after construction is basically evaluated by the performance in terms of uneveness index and structural stability over a period of time. The evaluation of these two important parameters will facilitate the clear understanding of performance of various materials used in the construction and to undertake suitable rehabilitation measures if necessary.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None
ID: 59093
Title: Effects of Biofertilizers on the Growth of Zea Mays L.
Author: M Iwuagwu, K S Chukwuka, U N Uka, M C Amandianeze.
Editor: P K Wong, R K Trivedi.
Year: 2013
Publisher: Global Science Publications.
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental Sciences Vol 15(no. 2), pp. 235-240, 2013
Subject: Asian Journal of Microbiology, Biotechnology & Environmental Sciences
Keywords: Biofertilizers, Azotobacter species, Azospirillum species Phosphate solubilising micro-organism, Maize and Plant growth.
Abstract: In recent years, the number of chemical industries increased in order to meet up with the demand for fertilizers. These industries depend on fossil fuels; hence the cost of fertilizer is governed by the cost of fuels. In order to reduce the dependence on chemical fertilizers including their inability to condition the soil and their polluting effect on the environment has directed attention to other sources of soil fertilization to enhance maize production. Hence a study was carried out to determine the effect of microbial inoculants (biofertilizers) comprising Azotobacter species, Azospirillum species and phosphate solubilising micro-organism (PSM) on the growth of Zea mays L. The experiment was arranged in a completely randomized design with six treatments and four replicates. Treatment one T1 represents control, T2= Azosirillum inoculants, T3=Azobacter inoculants, T4=Phosphate Solubilizing organism, T5=Azotobacter+ Azospirullum+PSM, T6=Azotobacter+Azospirillum. The treatments were applied to the maize seedlings 10 days after planting. Parameters for growth such as plant height, root length, plant fresh weight, dry weight, stem base diameter and plant chlorophyll content were used to compare the vegetative growth of the seedlings on different treatment applications. The result showed that seedlings treated with microbial inoculants responded greatly when compared to the control. Analysis of variance showed that there was significant increase in height, root length, stem diameter, fresh and dry weight of seedlings on the application of the microbial inoculants. The result suggests that the biofertilizers enhances the growth of Zea mays and as such its usage should be encouraged because of it is ecofriendly.
Location: TE 12 New Biology Building
Literature cited 1: None
Literature cited 2: None