Enter records

ID: 59917
Title: SEA FOOD BACTERIAL PATHOGENS AND SUSTAINABLITY OF AQUACULTURE.
Author: Olumide Adedokun Odeyemi
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 833-837 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Aquaculture, Sea food, Sustainability.
Abstract: Currently, aquaculture is the world fastest growing food economy with yearly increase of 10 % and thereby contributing over 29 % of total fish consumed in the world. It is mostly practiced in Southeast Asia with China taking the lead. Countries from Africa, South America and Asia Pacific are also engaging in aquaculture farming. Fishing constitutes the major source of generating income for over 35 % of the world inhabitants living along the coastline of the oceans hence, only a fraction of farmers from developing countries engage in small scale fisheries out of over 40 million people globally earning income from aquaculture. The global growth of aquaculture has been affected by various microbial diseases mostly from bacteria. These pathogenic bacteria are found in the culture water, sediment and sea foods. This paper therefore highlights some of the most common pathogenic bacteria affecting the sustainability of aquaculture in developing countries.
Location: TE 15 New Biology Building
Literature cited 1: Alvandi S.V. and Anathan, S. 2003. Biochemical characteristics, serogroups, and virulence factors of Aeromonas species isolated from cases of diarrhea and domestic water samples in Chennai.Ind.J. Med. Microbiol. 21: 233-238. Adebayo-Tayo, B.C., Okonko, I.O., John, M.O., Odu, N.N., Nwanze, J.C. and Ezediokpu, M.N. 2012. Occurrence of potentially Pathogenic Vibrio Species in Sea Foods Obtained from Oron Creek.
Literature cited 2: Buschmann, Ah, Tomova, A., Lo ' Pez A, Maldonado Ma, Henri ' Quez La, Et Al. 2012 Salmon aquaculture and antimicrobial resistance in the marine environment. PL0S ONE. 7 (8): e42724. Doi: 10.1371/journal.pone.00422724. Bondad-Reantaso, M.G. and Subasinghe, R.P. Aquatic animal health management in Asia. In: Walker, P., Lester, R., Reantaso, M.B. (Eds). Fifth Symposium on Diseases in Asian Aquaculture (DAAV). Fish Health Section, Asian Fisheries Society, Manila, Philippines.

ID: 59916
Title: GROWTH AND PHYSIOLOGICAL ATTRIBUTES OF WHEAT IN ZN-CONTAMINATED SOILS.
Author: Sanghpriya Gautam and S.N. Pandey
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 829-832 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Available zinc, Biochemical constituents, Antioxidative defence systems, Tritium aestivum, Zinc-contaminated soil.
Abstract: Zn-contaminated soils were rated for various levels of available (DTPA extractable) Zn viz. 0.42, 2.8, 4.5, 9.6 and 26.3 ppm and used to grow wheat (Triticum aestivum Linn) plants. Effect of these soils on growth (length and dry matter production), visible symptoms of toxicity and biochemical constituents (protein, pigments and sugar contents and catalase activity) of wheat were evaluated. Plants grown at high Zn-contaminated soil (26.3 ppm) had visible symptoms of toxicity such as decreased growth, chlorosis and tip burning of young leaves and reduced leaf lamina. Length and dry matter yield of wheat were increased maximum at 4.5 ppm available Zn, whereas these values decreased with increase in Zn levels in soil. Antioxidative defense systems with respect to carotenoids and protein contents and catalase activity favoured the dry weight production in wheat which were grown at 4.5 ppm of available Zn in soil. Study revealed the tolerance of wheat found maximum at 4.5 ppm of available Zn in soil, where as tissue concentrations of root and shoot were 30.6 and 35.6 ?g Zn g-1 of dry weight, respectively. High Zn concentrations (9.6 and 26.3 ppm) were not simulatory to the wheat growth reduced biochemical constituents in cells and produced visible symptoms of toxicity in wheat.
Location: TE 15 New Biology Building
Literature cited 1: Agarwala, S.C. and Sharma, C.P. 1979. Recognising micronutrient disorders of crop plants on the basis of visible symptoms and plant analysis. Botany Department, University of Lucknow, Lucknow India. pp 1-13. Berg, J.M., and Shi, Y. 1996. The galvanization of biology: a growing appreaction for the role of zinc. Science. 271: 1081-1085.
Literature cited 2: Bettger, W.J. and O ' Dell, B.L.A. 1981. Critical physiological role of zinc in structure and function of biomembranes. Life Sci. 28: 1425-1438. Bisht, S.S. 1976. Effect of heavy metals on plant metabolism. Ph.D. Thesis, University of Lucknow, Lucknow India.

ID: 59915
Title: DECOLORIZATION AND BIODEGRADATION OF SYNTHETIC DYES INDUSTRIAL EFFLUENTS BY ASPERGILLUS NIGER AND PENICILLIUM CHRYSOGENUM.
Author: Ramalingam, Muthu Dharani, Saraswathy and Saravanan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 823-827 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Decolorization, Biodegradation, Synthetic dyes, Textile dyeing effluent, Aspergillus niger, Penicillium chrysogenum.
Abstract: The objective of the study is to check the degradation and decolourisation ability of eight most commonly used synthetic dyes and industrial effluents by two fungal isolates Aspergillus niger and Penicillum chrysogenum. Malachite green, Acid green, Congo red, Red F2B, Blue FN3G, Commassie Brilliant Blue, Bromophenol Blue and Direct Violet were used. Dyes in the dye bath, hot wash, and cold wash liquors of textile dyeing effluents were degraded and decolorized by both A. niger and P. chrysogenum. Decolourization of dyes in textile dyeing effluents by these fungi took place in two stages: (i) adsorption of the dyes onto the surface of the biomass, and (ii) degradation of dyes. UV-Visible spectrophotometer analysis showed that all dyes were completely decolorized and degraded after 72 h using A. niger and 120 h using P.chrysogenum. Both A.niger and P.chrysogenum were found to be efficient for the degradation of dyes in dyebath, hotwash liquor, cold wash liquor-1 and cold wash liquor-2 of textile-dyeing effluents and these fungi can be exploited industrially to address the pollution problems.
Location: TE 15 New Biology Building
Literature cited 1: Ali, N., Abdul, H., Muhammad, F.S., Pir, B.G. and Safia, A. 2009. Application of Aspergillus niger SA1 for the enhanced bioremoval of azo dyes in simulated textile effluent. African J. Biotechnol. 8: 3839-3845. Eichlerova, I., Homolka, L., Lisa L., and Nerud F. 2006. The influence of extracellular H2O2 production on decolorization ability in fungi. J.Basic Microbiol. 46: 449-455.
Literature cited 2: Fu, Y. and Viraraghavan T. 2001. Fungal decoloization of dye wastewaters. Review. Bioresouce Technol. 79: 251-262. Kuhad, R.C., Sood, N., Tripathi, K.K., Singh A. and Ward O.P. 2004. Developments in microbial methods for the treatment of dye effluents. Adv.Appl.Microbiol. 56: 185-213.

ID: 59914
Title: HIGH-LEVEL SOLUBLE EXPRESSION OF BOVINE ENTEROKINASE CATALYTIC LIGHT CHAIN WITH NUS-TAG IN ESCHERICHIA COLI .
Author: Mahammad Azhar and R.Somashekhar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 815-821 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Enterokinase light chain, Gly-(Asp) 4- Lys-?-naphthylamide, Inclusion bodies, Nus tag, Soluble form, Trx tag.
Abstract: Enterokinase (E.C.3.4.21.9) acts as a sequence-specific protease. It activates trypsinogen by cleaving its inhibitory portion. Enterokinase as wide utility in cleaving fusion proteins. Bovine Enterokinase light chain (bEKL) encoding nucleotide sequence was isolated from Bos taurus indicus (Gene Bank Accession No. KC756844) and one amino acid mutation (Proline 121 Serine) was identified. Bovine Enterokinase light chain is prone to form inclusion bodies when expressed in Escherichia coli (E. coli). The BekL gene was inserted into pET21a, pET32a and pET43a expression vectors, and fused to T7 tag (The initial 11 amono acids of the T7 gene 10 protein), thiredoxin (Trx) tag and Nus tag (N-utilizing substance A) respectively. The recombinant bovine Enterokinase light chain (rEKL) constructs were expressed in E.Coli Rosetta strain, and it was observed that T7-BekL, Trx-bEKL expressing mainly as an inclusion body, but Nus-bEKL most of the fusion protein existed in soluble form. High level of bovine Enterokinase light chain-Nus tag protein production was achieved (200 mg) from a 1 litre culture. 120 mg protein was isolated from a 1 liter culture. After cleavage, 28 mg of purte active catalytic light chain was obtained. The rEKL was showing Km value 0.65 mM and Kcat 1500 min?1 for fluorometric substrate Gly-(Asp) 4 - Lys-?-naphthylamide. These values were found similar to previously published data for bovine EK light chain, and showed that mutation has no effect on its enzyme activity. Nus tag has increased EKL Solubility and Expression levels.
Location: TE 15 New Biology Building
Literature cited 1: Affinity Chromatography Principles and Methods, Handbooks from GE Healthcare. Bricteux-Gregoire, S., Schyns, R. and Florkin, M. 1972. Phylogeny of trypsinogen activation peptides, Comp. Biochem. Physiol. 42B: 23-29.
Literature cited 2: Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162 (1): 156-159. Chomczynski, P., et al. 1994. A reagent for the single-biopsy samples. J. NIH. Res. 6: 83.

ID: 59913
Title: STATISTICAL SCREENING OF MEDIUM COMPONENT BY PLACKETT-BURMAN EXPERIMENTAL DESIGN FOR BIOSURFACTANT PRODUCTION BY INDONESIAN INDIGENOUS BACILLUS SP DSW17
Author: Swastika Praharyawan, Dwi Susilaningsih and Khaswar Syamsu.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 805-813 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Biosurfactant, Biosurfactant production, Statistical optimization, Plackett-Burman, Bacillus.
Abstract: Statistcal screening of media components for biosurfactant production by Indonesian indigenous of Bacillus sp DSW17 was carried out using Plackett-Burman experimental design. Nine out of eleven factors of the production medium were found to be significantly affecting the production process of biosurfactant. FeSO4. 7H2O, NaNO3, frying oil waste, CaCl2 .2H2O, K2HPO4 and sucrose were directly proportional to the biosurfactant production while ZnSO4.7H2O, KH2PO4 and MgSO4. 7H2 showed inversely proportional correlation with the biosurfactant production in the selected experimental range. Several modified mediums were formulated based on the statistical screening result in order to validate the optimum media composition in producing biosurfactant. Modified medium combination 1 (MM1) as an optimized medium (2.13 g/ L) showed 124 % increase in biosurfactant production over the unoptimized medium or MM4 (0.95 g/L).
Location: TE 15 New Biology Building
Literature cited 1: Abouseoud, M., Maachi, R., Amrane, A., Boudergua, S. and Nabi A. 2008. Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescencens. Desalination. 223: 143-151. Atmaca, S., Gul, K., and Cicek, R., 1998. The effect of zinc on microbial growth. Tr J Med Sci. 28: 595-597.
Literature cited 2: Bong, C.W., Malfatti, F., Azam, F., Obayashi, Y. and Suzuki, S. 2010. The effect of zinc exposure on the bacteria abundance and proteolytic activity in seawater. In: Hamamura N, Suzuki S, Mendo, S., Barroso, M., Iwata, H. and Tanabe, S. (Eds), Interdisciplinary Studies on Environmental Chemistry-Biological Response to Contaminants. Pp 57-63. Carvalho, C.M.L., Serralheiro, M.L.M., Cabral, J.M.S. and Airewbarros, M.R. 1997. Application of factorial design to the study of transestirification reactions using cutinase in AAOT-reversed micelles. Enzyme Microbial Technol. 27: 117-123.

ID: 59912
Title: A NOVEL METHOD OF USING BIOSENSOR WITH NANOPARTICLES FOR THE DETECTION OF PATHOGENIC BACRTERIA IN FOOD.
Author: V. Manonmani, A.Vimala Juliet and K.Praveen Kumar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 799-804 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Nano biosensor, uv-Vis spectroscopy, Fluorescence spectroscopy, Mg Nps (Magnetic Nanoparticles), chitosan-magnetite nanocomposite particles, Optical density (OD), FLU (Fluorescence signal)
Abstract: A key step in food borne pathogens control s to effectively detect pathogens along food production and processing line in a timely manner. Conventional methods range from color based to fluorescence based, from immunology based to PCR based tests. These approaches being potential most of them are time consuming and difficult. Nanoparticles are usually used either as labels or separation aids or both in pathogen detection procedures because of unique optical, electrical or magnetic properties. In this paper, a new model of biosensor was proposed which offers several benefits over existing methodologies. Biosensors emerged with nanotechnology approaches, holds great challenge for addressing the needs of medical diagnostic systems. In this study, we developed a Nano Biosensor which consists of various stages such as synthesis of Magnetic nanoparticles, it ' s surface modification with chitosan, characterization using UV-Visible Spectroscopy and TEM, dye conjugation (Rhodamine and congo red), and finally the detection of bacteria using fluorescence spectroscopy. The basic concept behind the work is that the chitosan, a biodegradable polymer from amine group is used for separation of pathogenic bacteria which, specifically binds onto the surface of bacteria cell wall due to attraction of electrostatic charges and protein-receptor interactions. Thus the obtained chitosan magnetite complex was recovered from reaction mixture by using an external magnet. And then, the surface modified bacteria ' s were separated and incubated at 37 ? C for few minutes. T his test sample is being conjugated finally with 10 ?l of rhodamine dye which will emit light upon input source light. Similarly, test sample being conjugated with congo red dye also. Bacterial presence was being detected and measured in terms of fluorescence intensity using biosensor scheme.
Location: TE 15 New Biology Building
Literature cited 1: Briza P _ erez-L_ opeza and Arben Merkoc. 2011. Nanomaterials based biosensors for food analysis applications. Trends in Food Science & Technology. 22: 625-639 Chen and Zhang, 2012. Bioconjugated Magnetic Nanoparticles for Rapid Capture of Gram-positive Bacteria. J. Biosens Bioelectron. S: 11.
Literature cited 2: George G. Daaboul, Carlos A. Lopez, Abdulkadir Yurt. 2012. Label-Free Optical Biosensors for Virus Detection and Characterization. IEEE Journal of selected topics in quantum electronics. 18: 4 Hyosook Lee, Huiping Shao, Yuqiang Huang and Byung Kook Kwak. 2005. Synthesis of MRI Contrast Agent by Coating Superparamagnetic Iron Oxide with Chitosan. IEEE Transactions on Magnetic. 41: 10

ID: 59911
Title: CONSERVATION STRATEGIES FOR MELIA DUBIA: A PROSPECTIVE TREE FOR MULTIPURPOSE USE.
Author: Aditya Kumar and Rameshwar Das.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 795-797 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Agroforestry, Germplasm Conservation, Pulp wood, Timber, Medicinal value.
Abstract: Melia Dubia Cav. (syn.M.composita), the fastest growing tree belongs to the family Meliaceae. It has the potential to be utilized as energy crop, pulp wood, ply wood, timber and also as medicine. This species is native to India and widely distributed in the tropical moist deciduous forests. The utilization of the species is widely taken up in the plains of India as a short rotation crop for pulp and paper industries and also as a potent species under agroforestry systems. The potential of these species is not yet utilized in North-Eastern region, whereas, it is being harvested in a disorganized manner leading to fast disappearance. Its conservation in natural habitat and also outside is very much essential to meet the demand of the people with the changing land use pattern. This study discusses the various strategies required to conserve germplasm of the species.
Location: TE 15 New Biology Building
Literature cited 1: Bortokini, T.I., A.U. Okere, A.O. Giwal, B.O. Daramola and W.T. Odofin, 2010. Biodiversity and conservation of plant genetic resources in Field Genebank of the National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria. International Journal of Biodiversity and Conservation. 2 (3): 037-050 pp. Carpinella, M.C., M.T. Defago, G. Valladares, and S.A. Palacios, 2003. Antifeedant and Insecticide Properties of a Limonoid from Melia azedarach (Meliaceae) with Potential Use for Pest Management. J.Agric.Food Chem. 51: 369-374.
Literature cited 2: Chiffelle, I. Huerta, A., Azua, F., Puga, K. and Araya, J.E. 2011. Antifeeding and insecticide properties of aqueous and ethanolic fruit extracts from melia azedarach L. on the elm Leaf beetle Xanthogaleruca luteola Muller. Chilean Journal of Agricultural Research 71 (2): 218-225. Chinnaraj, S., and C. Malimuthu, 2011. Development of micro-propagation and mini cutting protocol for fast growing Melia, Dalbergia and Eucalyptus clones for pulpwood and bio-energy plantations.

ID: 59910
Title: MOLECULAR DETECTION OF OCHRATOXIN PRODUCING FUNGAL GENE, POLYKETIDE SYNTHASE GENE (PKS) IN INFECTED MAIZE SAMPLES USING PCR.
Author: Nagalakhshmi S, Manorama K, Anurag Chaturvedi , Shreedhar M, Krishna Bhagavatula Mr, Sunil-Chidambar K, Ravicharan A, Prathima N, Durgarani Ch. V and Reddy Vln.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 789-793 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Maize, Ochratoxin, Bioanalyzer and Normal-PCR
Abstract: Corn production was estimated a production of around 790 million tones. Most of the corn is used for feed, food, and seed, maize (Zea mays L.) is one of the cereals which serves as a main source of food, forage and processed products for industry. As a staple food it provides more than one-third of the calories and proteins in some countries. Ochratoxins are cyclic polyketides, the most potent of which is ochratoxin A (OTA). OTA has been associated with human endemic neuropathy. Large number of other Penicillium and Aspergillus species have since been reported to be OTA producers. The present study was undertaken with the main aim of detecting the presence of the genes encoding the production of mycotoxin, namely, ochratoxin in the infected maize samples. One hundred and thirty maize samples infected with the fungi Aspergillus and Penicillium were collected from the markets, godowns and farmers ' fields. DNA isolated from such maize samples was amplified in PCR using sets of forward and reverse gene specific primers designed using DNASTAR Lasergene, pks. Fragments. A 200 base pair fragments were amplified in eleven samples. This study helps in easy detection of mycotoxins present in the contaminated samples in storage which are unfit for consumption thereby avoiding the hazardous influence of such toxins on human and animal health.
Location: TE 15 New Biology Building
Literature cited 1: Abe, Y., Suziki, T., Mizuno, T., Ono, C., Iwamoto, K., Hosobuchi, M. and Yoshikawa, H. 2002. Effect of increased dosage of the ML-236B (compactin) biosynthetic gene cluster on ML-236B production in penicillium citrinum. Mol Genet Genomics. 268: 130-137. Bottalico, A. 1998. Fusarium diseases of cereals: species complex and related mycotoxin profiles, in Europe. Journal of Plant Pathology. 80: 85-103.
Literature cited 2: Brown, D.W., McCormick, S.P., Alexander, N.J., Proctor, R.H. and Desjardins, A.E. 2001. A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and Fusarium graminearum? Fungal Genetics and Biology 32: 121-133. Chandler, E.A., Simpson, D.R., Thomsett, M.A. and Nicholson, P. 2003. Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes and characterization of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis. Physiological and Molecular Plant Pathology.

ID: 59909
Title: ISOLATION OF POTENTIAL MICROBIAL PRODUCERS OF FRUCTOSYL TRANSFERASE FROM BAGGASSE AND SELECTED SOIL SITES OF CHHATTISGARH, INDIA.
Author: Seema A. Belorkar, A.K. Gupta and Vibhuti Rai.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 785-788 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Fructosyl transferase, Fructooligosaccharides and aspergillus niger.
Abstract: The present study focuses on microbiological ecology of varied soil samples which can be exploited as potential sources for isolation of industrially important microbes. Seven soil samples and one baggasse sample was selected as sources to isolate microbes which were screened further for production of Fructosyltransferease (Ftase) enzyme. Ftase is an enzyme tool for synthesis of Fructooligosaccharides (FOS). The undertaken study revealed that there is a significant variation in the average number of bacteria, molds and yeasts in various samples. The differences in their means were found to be statistically insignificant. The counts of microbes per gram of samples were found to be in expected proportions indicating that differences in sampling locations do not change the relative proportions indicating that differences in sampling locations do not change the relative proportions indicating that differences in sampling locations do not change the relative proportions of the types of microbes. The present study will also prove to be guideline for selection of sampling sites for future isolations intended towards Fructosyltransferease producing microbes.
Location: TE 15 New Biology Building
Literature cited 1: Amir, H. and Pineau, R. 1998. Influence of plants and cropping on microbiology characteristics of some new Caledonian Ultramafic soils. Aust. J. Soil Res. 36: 457-470. Atlas, R.M., Bartha, R. 1998. Microbial Ecology: Fundamentals and Applications. 4th Edition, Benjamin Cummings Publishing Company Inc., Addison, Wesley Longman Inc., pp. 300-350.
Literature cited 2: Cho, W.T., Lim, J.Y. and Lee, S.S. 1990. Isolation and identification of the black Yeast producing Fructosyl transferase. Kor. J. Mycol. 18: 20-25. Fawkia, M.B., Fattah, A.M.A., Hasanein, D.A., Mustafa, F.A.and Fatta, F.FA. 2009. Production and Some Properties of Fructosyltransferease from Bacillus Cereus. Applied Sciences Research. 5: 1132-1141.

ID: 59908
Title: INFLUENCE OF CATHODE ELECTRON ACCEPTORS AND ANODE MICROBES IMPROVING ELECTRICITY GENERATION IN DUAL-CHAMBERED MICROBIAL FUEL CELL USING DISTILLERY WASTEWATER.
Author: K.Geetha and S.Amal Raj.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 779-784 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Distillery Wastewater, Salt bridge, Mixed consortium, Electron acceptor, Dissolved oxygen.
Abstract: In this investigation a dual-chambered microbial fuel cell (MFC) with a salt bridge separator as a proton exchange membrane (PEM) was used to harness bioelectricity from primary treated distillery wastewater. The study examines the activity of various cathodic electron acceptors using chemicals such as potassium permanganate (KMnO4), potassium ferricyanide (K3FeCN6) and dissolved oxygen (DO) in a cathode chamber. Among the three different electron acceptors, KMnO4 possesses high oxidation capacity, which results in a maximum power output of 6.26 m W/m2 than K3FeCN6 (1.280 mW/m2) and dissolved oxygen (0.83 m W/m2). The biocatalytic activity of the microbial community for electron transport from the substrate to the anode electrode were also studied using a strain (SS) and distillery-enriched mixed consortium (MC) based on the cell potential. Compared to the single strain the mixed consortium consumes more complex carbon sources and accepts wider substrates, which increases the cell potential.
Location: TE 15 New Biology Building
Literature cited 1: APHA. 1998. Standard Methods for Examination of Water and Wastewater, 20th ed. American Public Health Association/ American Water Works Association/ Water Pollution Control Federation, Washington, DC. Behera, M. and Ghangrekar, M.M. 2009. Performance of microbial fuel cell in response to change in sludge loading rate at different anodic feed pH. Bioresour. Technol. 100 (21): 5114-5121.
Literature cited 2: Chae, K.J., Choi, M.J., Lee, J.W., Kim, K.Y. and Kim, I.S. 2009. Effect of different substrates on the performance, bacterial diversity, and bacterial viability in microbial fuel cells. Bioresour. Technol. 100 (120: 3518-3525. Chang, I.S. Jang, J.K., Gil, G.C., Kim, M., H.J. Cho, B.W. and Kim, B.H. 2004. Continuous determination of biochemical oxygen demand using microbial fuel cell type Biosensor. Biosens Bioelectron. 19 (6): 607-613

ID: 59907
Title: POTENTIATION OF ANTIBIOTIC-OXACILLIN BY CITRIC ACID AGAINST METHICILLIN RESISTANT STAPHYLOCOCCI.
Author: N.A. Chandak, B.J.Wadher and S.R. Deshmukh
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 773-778 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Methicillin Resistant Staphylococci, Potentiation, Citric acid.
Abstract: In recent years, the environmental contamination caused by excessive use of chemical pesticide increased the interests in integrated pest management, where chemical pesticides are substituted by bio-pesticides to control plant pests diseases. Trichoderma viride is an ecofriendly biological fungicide. In the present study different types of polished and unpolished rice were used to prepare rice wash for cultivation of Trichoderma viride and its maximum yield were recorded in rice wash of unpolished rice and the lowest yield in polished rice. The cultivated Trichoderma viride were used as pesticide and its field trials were conducted on one Acre of land. Trichoderma viride enhanced the root and plant development, solubilisation of inorganic nutrients and induced resistance inactivation of pathogens enzymes might be due to secreting certain plant growth stimulating substances.
Location: TE 15 New Biology Building
Literature cited 1: Alagarsamy, G. and Sivaprakasam, K. 1988. Effect of antagonists in combination with carbendazim against Macrophomina phasiolina infection in cowpea. Journal of Biol.Control. 2: 123-125. Aneja, K.R. 2003. Experiment in Microbiology, Plant Pathology and Biotechnology (4th Edition), New Age International Publication.
Literature cited 2: Bailey, D.J., Kleczkowski, A. and Gilligan, C.A. 2004. Epidemiology dynamics and the efficiency of biological control of soil-borne diseases during consecutive epidemics in a control environment. New Phytopathology. 161: 569-576. Chaudhari, P.J., Shrivastava, P. and Khadse, A.C. 2011. Substrate evaluation for mass cultivatrion of Trichoderma viride. Asiatic Journal of Biotechnology Resources. 2 (4): 441-446.

ID: 59906
Title: CULTIVATION OF TRICHODERMA SPECIES ON RICE WASH AND ITS APPLICATION IN PEST CONTROLLING.
Author: P.B. Arekar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 769-772 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Trichoderma viride, Rice wash, Cotton plant.
Abstract: In recent years, the environmental contamination caused by excessive use of chemical pesticides increased the interests in integrated pest management, where chemical pesticides are substituted by bio-pesticides to control plant pests and plant diseases. Trichoderma viride is an ecofriendly biological fungicide. In the present study different types of polished and unpolished rice were used to prepare rice wash for cultivation of Trichoderma viride and its maximum yield were recorded in rice wash of unpolished rice and the lowest yield in polished rice. The cultivated Trichoderma viride were used as pesticide and its field trials were conducted on one Acre of land. Trichoderma viride enhanced the root and plant development, solubilisation of inorganic nutrients and induced resistance inactivation of pathogens enzymes might be due to secreting certain plant growth stimulating substances.
Location: TE 15 New Biology Building
Literature cited 1: Alagarsamy, G. and Sivaprakasam, K. 1988. Effect of antagonists in combination with carbendazim against Macrophomina phasiolina infection in cowpea. Journal of Biol. Control. 2: 123-125. Aneja, K.R. 2003. Experiment in Microbiology, Plant Pathology and Biotechnology (4th Edition), New Age International Publication.
Literature cited 2: Bailey, D.J., Kleczkowski, A. and Gilligan, C.A. 2004. Epdermiological dynamics and the efficiency of biological control of soil-borne diseases during consecutive epidemics in a control environment. New Phytopathology. 161: 569-576. Chaudhari, P.J., Shrivastava, P. and Khadse, A.C. 2011. Substrate evaluation for mass cultivation of Trichoderma viride. Asiatic Journal of Biotechnology Resources. 2 (4): 441-446.

ID: 59905
Title: ASSESSMENT OF DIFFERENT PARTS OF ANNONA SQUAMOSA L. FOR THEIR ANTIMICROBIAL ACTIVITY AGAINST PATHOGEN ISOLATES FROM RAIPUR REGION.
Author: Rinu Khan and A.K. Gupta
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 765-768 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antimicrobial activity, Agar diffusion method, Gram-negative pathogens, Gram positive pathogen, Annona squamosa, Leaf, Stem, Root.
Abstract: Annona Squamosa L. member of the Annonaceae family, also known as custard apple, is commonly found in deciduous forests and is cultivated in wild in various parts of India. In this investigation, antimicrobial effect of root, seed and leaves extracts of Annona squamosa L. on clinical isolates of three Gram negative and one Gram positive pathogens from Raipur Region were studied, using paper disc method. Gram negative and positive microorganisms studied were Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus. For examination of antimicrobial properties of A. squamosa aeruginosa and Staphylococcus aureus. For examination of antimicrobial properties of A. squamosa on pathogenic microorganisms, disc diffusion method using different parts of the plant extracted with four different solvents namely petroleum ether, acetone, ethyl alcohol and water were used. Among all, the root of Annona squamosa was found to be more pronounced in Pseudomonas aeruginosa (7.66 ? 0.33) for the acetone extraction of root of Annona squamosa was found to be more effective as compared from seed and leaf. The zone of inhibition was found to be more pronounced in Pseudomonas aeruginosa (7.66 ? 0.33) for the acetone extraction of root of A. squamosa followed by Escherichia coli (6.66 ? 1.20), S. aureus (4.66 ? 0.88) and Klebsiella pneumonia. Seeds of A. squamosa also showed similar pattern of inhibitory effects on tested organism lesser than that of root. The leaf of this plant showed lower antimicrobial potential compared from root and seed. Aqueous extract did not express satisfactory result against clinical isolates. The activity index of root of A. squamosa was found to be greater as compared to the activity index of the seed and leaf. The phytochemical analysis revealed that the plant is rich in chemicals such as alkaloids, flavonoids, saponins, sterols, tannins and terpenoids. The results suggested that acetone could be effectively used for the extraction of phyto compounds from A. squamosa. Similarly the root of the plant could be used as important source for antimicrobial property against clinical isolates.
Location: TE 15 New Biology Building
Literature cited 1: Basri, D.F., Sharif, R., Morat, P. and Latip, J. 2005. Evaluation of antimicrobial activities of the crude extracts from Garcinia atroviridis and Solanum torvum. Malaysian J. Sci, 24: 233-238. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck, M. 1996. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45: 493-496.
Literature cited 2: Betoni, J.E.C., Mantovani, R.P., Barbosa, L.N., Di-Stasi, L.C. and Fernandes, A. 2006. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases.Mem.Inst. Oswaldo Cruz. 101 No. 4. De-Omena, M.C., Bento, E.S., De Paula, J.E. and Sant Ana, A.E.G. 2006. Larvicidal diterpens from Pterodon Polygalaefloras. Vector Borne and Zoonotic Dis. 6: 216-222.

ID: 59904
Title: EVALUATION OF GENETIC DIVERSITY IN SOME BANANA HYBRIDS USING RAPD MARKERS.
Author: Sukhen Chandra Das and T.N Balamohan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 759-764 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Hybrids, Cluster analysis, Polymorphism, RAPD, Dendrogram.
Abstract: Bananas (Musa spp.) are mostly diploid or triploid with various combinations of genomes inherited from their ancestors. Random amplified polymorphic DNA (RAPD) markers were used for evaluation of genetic diversity of some synthetic Banana Hybrids. A total of 13 random primers were evaluated for banana hybrids based on RAPD polymorphism. Diversity analysis carried out by using RAPD markers n some Banana hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship of the hybrids with their parents. Out of the 13 primers studied, all the primers exhibited scorable markers. However, the maximum number of loci amplified was 18 with primer OPZ 12 and the maximum polymorphism percentage was obtained with the primer OPZ 16.
Location: TE 15 New Biology Building
Literature cited 1: Bhakthavatsalu, C.M. and Sathiamoorthy, S. 1979. Banana clonal situation in India: A resume. Fruits. 11: 34-46. Bhat, K.V., Bhat, S.R. and Chandel, K.P.S. 1992. Survey of isozyme polymorphisms and catalase. Journal of Horticultural Science.67: 501-507.
Literature cited 2: Bhat, K.V. and jarret, R.L and Liu, Z.W. 1994. RFLP characterization on Indian Musa germplasm for clonal identification and classification. Euphytica. 80: 95-103. Bhat, K.V. and Jarret, R.L. 1995. Random amplified polymorphic DNA and genetic diversity in India Musa germplasm. Genetic Resources Crop Evoluation. 42: 107-118. Record No: 60341

ID: 59903
Title: STUDY ON ANTIBACTERIAL ACTIVITY OF HONEY SAMPLES COLLECTED FROM MEDICINAL PLANT SOURCES.
Author: Karuna Pawar, Kedar Rokade and Gajanan Mali.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 755-758 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antibacterial activity, Honey, Agar diffusion method
Abstract: The present investigation describes about the antibacterial activity of three different natural honey samples obtained from different locations of Sangli district. The pH values of these collected honey samples ranges from 4.0 to 4.5. Antibacterial activity of honey at its natural acidic pH as well as by maintaining neutral pH was studied on seven pathogenic bacteria. Marked variations were observed in the antibacterial activity of these honey samples. All the honey samples at natural acidic pH as well as at neutral pH showed a spectrum of antibacterial activity on five different bacterial species such as Salmonella typhi, Shigella dysentriae, Bacillus megaterium, Staphylococcus aureus, and Proteus vulgaris.
Location: TE 15 New Biology Building
Literature cited 1: Al-Walli, N.S., Akmal, M., Al-Walli, F.S., Saloom, K.Y. and Ali, A. 2005. The antimicrobial potential of honey from United Arab Emirates on some microbial isolates. Media Science Monitor. 11: 433-438 Baur, A.W., Kirby, WM.M, Sherris, J.C. and Turck, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical pathology. 36: 493-496.
Literature cited 2: Carmo-Sousa, L., Rose, A.H. and Harison, J.S., 1969. The Yeasts. Academic Press, London. 1: 79-105. Cooper, R.A., Molan, P.C. and Harding, K.G. 1999. Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds. Journal Royal Society of Medicine. 92: 283-285.