Enter records

ID: 59932
Title: Effect of pesticide on metamorphosis of frog tadpole, Euphlyctis cynophlyctis.
Author: V.I. Kalamade.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 855-858 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Amphibian, Bioindicators, Pesticide, Hamla, Insect pest, Histological.
Abstract: Decline in the amphibian population has been noted since 1980s from all over the world. Thirty two percent of species are globally threatened nine to twelve species have become extinct since 1980s. This decline is perceived as one of the most critical threats to global biodiversity and environmental imbalance. Several causes are believed to be involved, including diseases, habitat destruction and modification, exploitation, pollution, pesticide use, climate change and increased UV radiation. However many of the causes of amphibian decline are still poorly understood. Contamination of environmental factors by pollutants have resulted in alarming decline in Amphibian as a bioindicators of environment factors by pollutants have resulted in alarming decline in Amphibian population, this fact has highlighted the importance of using Amphibians as a bioindicators of environmental pollution, this fact has highlighted the importance of using Amphibians species breed in rice field, but the pesticide Hamla (Trade name), which is the mixture of Chlorpyrifos and cypermethane, is sprayed to eradicate insect pest on rice. This may contaminate the breeding water to the harmful level. The present work had been carried out to study effect of the pesticide Hamla on metamorphosis of tadpole of Frog (Euphylyctis cynophlyctis) and on histological structure of intestine of tadpole treated with sub lethal levels of Hamla. Our results showed that sub lethal concentrations of Hamla arrested metamorphosis of tadpole and damaged the histological structure of intestine. It appears Hamla is one of the chemical agents responsible for alarming decline in Amphibian population.
Location: TE 15 New Biology Building
Literature cited 1: Cowman, D.F. and Mazanti, L.E. 2000. Ecitoxicology of new generation pesticides to Amphibians. In: Sparlinng, D.W., Linder, G., Bishop, C.A. (Eds) Ecotoxicology of Amphibians and Reptiles: SETAC Press, Pensacola, F.L, 233-268. Davidson, C. 2004. Declining downwind: Amphibian population declines in California and historical pesticide use. Ecological Applications. 14: 1892-1902.
Literature cited 2: Elimann, G.L., Courtney, K.D., Andres Jr., V. And Featherstone, R.M., 1961. A new and rapid colorometric determination of Acetylcholine-esterase metric determination of Acetylcholine-esterase activity. Biochemistry and pharmacology 7: 88-95. Fellers, G.M., McConnell, L.L., Preatt, D and Datta, S. 2003. Pesticides in mountain yellow-legged frogs (Rana mucosa) from the Sierra Nevada mountrains of California USA. Environmental Toxicology and Chemistry. 20: 179-184 (2000).

ID: 59931
Title: Molecular identity for the elite rice variety, Samba Mahsuri (BPT 5204) using SSR markers.
Author: P. Sravanthi, CH.V.Durga Rani, S. Nagalakshmi and M. Madhavi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 847-854 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Samba Mahusuri, Rice (Oryza sativa L.)
Abstract: The investigation for genetic purity testing of Samba Mahusuri in rice (Oryza sativa L) using microsatellite markers was conducted to identify the signature markers to Samba Mahusuri and also to replace grow out test, which has been used conventionally for seed purity testing through DNA based assays. 40 SSR markers were used to screen the samba mahsuri (BPT 5204). About 38 popular rice varieties including Samba Mahsuri (BPT5204) were used in the present study. And SSR marker RM 11278 could efficiently used to generate molecular IDs for samba Mahsuri BPT 5204 respectively.
Location: TE 15 New Biology Building
Literature cited 1: Arunakumara, K., Durga Rani, Ch. V., Sivaramakrishnan, S., Sundaram, R.M and Vanisree, S. 2008. Assessment of genetic purity in rice cultivar, Samba Mahsuri (BPT 5204) using SSR markers.M.sc Ag (thesis) ANGRAU (Acharya N.G. Ranga Agricultural University ). Botstein, D., White, R.L., Skolnick, M and Davis, R.W. 1980. Construction of genetic linkage map in man using restriction fragment length polymorphism (RFLP). American Journal of Human Genetics. 32: 314-331.
Literature cited 2: Byerlee, D. 1996. Knowledge-intensive crop management technologies: Concepts, impacts and prospects in Asian agriculture. International Rice Research Conference, Bangkok, Thailand. Causse, M.A., Fulton, T.M., Cho, Y.G., Ahn, S.N., Ronald, P.C., Harrington, S.E., Second, G., McCouch, S.R. and Tanksley, S.D. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics.138: 1251-1274.

ID: 59930
Title: Experimental investigation of enhanced production of pectinase by immobilized Aspergillus Niger using low cost substrate.
Author: N.Kavitha, V.Sudarshana Deepa, T. Chitra Devi and R. Ravikumar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 841-846 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pectinase, Immobilized Aspergillus niger, Carrot, Corn, Submerged fermentation.
Abstract: The present study investigated the production of pectinase by immobilized Aspergillus niger NFCCI 2822 in submerged fermentation using low cost substrates of carrot and corn. Batch experiment was conducted at 30 ? C with various substrate concentrations at pH 4.0 with different time intervals to obtain the maximum yield of Pectinase. Results revealed that the medium containing carrot medium which was partially purified had the maximum enzyme activity of 147.02 U/mL than enzyme produced by the corn medium of 103.68 U/mL.Adinarayana, K., Jyothi, B. and Ellaih, P. 2005. Production of alkaline protease with immobilized cells of Bacillus subtilis PE-11 in various matrices by entrapment technique. AAPS Pharmaceutical Science and Technology. 6 (3): 391-397.
Location: TE 15 New Biology Building
Literature cited 1: Adinarayana, K., Jyothi, B. and Ellaih, P. 2005. Production of alkaline protease with immobilized cells of Bacilus subtilis PE-11 in various matrices by entrapment technique. AAPS Pharmaceutical Science and Technology. 6 (3): 391-397. Aguilar, G. and Huirton, C. 1990. Constitutive exopectinase produced by Aspergillus sp. CH-Y-1043 on different carbohydrate source. Biotechnology Letters. 12: 655-660.
Literature cited 2: Akhter, N., Alam Morshed, M.A., Uddin, A., Begum, F., Sultan, T. and Azad, A.K. 2011. Production of Pectinase by Aspergillus niger Cultured in Solid State Media. International Journal of Biosciences. 1 (1): 33-42. Anna, V., Nigar, B. and Venko, B. 2013. Cyclodextrin glucanotransferase production by free and agar gel immobilized cells of B. circulans ATCC 21783. Process Biochemistry. 38: 1585-1591.

ID: 59929
Title: Impact of Environmental acidic pH on oxidative enzymes of fresh water fish, Cyprinus carpio (L)
Author: U.Srineetha, M. Venkata Reedy and M. Bhaskar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 835-839 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Acidic pH, Gill, Brain, Oxidative enzymes, Cyprinus carpio (L)
Abstract: In the present study the activities of oxidative enzymes like glucose-6-phosphate dehydrogenase (G-6-PDH), lactate dehydrogenase (LDH), glutamate dehydragenase (GDH), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) in gill and brain of Cyprinus carpio at 7th and 15th days of exposure to sub lethal concentration of acidic media (PH5.0) besides controls, are presented in Table 1 and 2. The activities of G-6-PDH, SDH and MDH decreased at day 7 and 15 in the gill and brain of experimental fish with a corresponding increased in the activities of LDH and GDH of experimental fish over control. But in between the two organs the degree of decrease was greater in gill than the brain of fish. Probably, more suppression of oxidative metabolism occurs in gills than the brain of fish. Interestingly, in the case of GDH activity was high in brain than the gill of fish.
Location: TE 15 New Biology Building
Literature cited 1: Bhaskar, M. and Govindappa, S. 1986. Effect of environmental activity and alkalinity on the physiology of Tilapia mossambica (Peters) during acclimation. Biochemical Systematics Ecology. 14: 439-443. Dheer, J.M.S., Dheer, T.R .and Mahajan, C.S. 1987.Haematological and haematopietic response to aid stress in an air breathing freshwater fish, Channa punctatus (Block). J. Fish. Biol. 26: 475-481.
Literature cited 2: Ghillebeart, F., Chaillou, C., Deschamps, F. and Rouboud, P. 1995. Toxic effects at three pH levels of two reference molecules on common carp embryo. Ecotoxicol Environ.Safe. 32: 19-28. Javed, M.H. Yousuf, F.A. Hussain, A.N., Ishaq, M. and Waqar, M.A. 1995. Purification and properties of LDH from liver of uromastix hardwickii. Comp. Biochem.Physiol. 11 (1B): 27-34.

ID: 59928
Title: Study of genomics data for developmental stage of shoot in Arabidopsis Thaliana using principal component analysis.
Author: Nitesh Chandra Mishra, Sunny Patel, Manish Sharma and Kiran Singh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 827-834 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Arabidopsis thaliana, K-means, Microarray, PCA.
Abstract: Time is moving for open and difficult challenges of survival process of living being in nature. We think everything about life depends on energy as nothing is beyond energy limits. Now this study is showing that High throughput gene expression analysis of shoot development in Arabidoopsis thaliana is becoming more and more important in many areas of biomedical research like functional genomics such as gene expression data analysis. cDNA microarray technology is one very promising approach for high throughout analysis and provides the opportunity to study gene expression patterns on a genomic scale. Thousands or even tens of thousands of genes can be spotted on a microscope slide and relative expression levels of each gene can be determined by measuring the fluorescence intensity of labeled mRNA hybridized to the arrays. Beyond simple discrimination of differentially expressed genes or diagnostic classification requires the clustering of genes from multiple experiments into groups with similar expression patterns. This information aims to identify relationships between the structural features, at the molecular level, of gene or gene products that predict the activity of the compound against the cell.
Location: TE 15 New Biology Building
Literature cited 1: Alexander Sturn 2000. Cluster Analysis for large Scale Gene Expression Studies. Arthur, D., Manthey, B. and Roeglin, H. 2009. K-means has polynomial smoothed complexity. Proceedings of the 50th Symposium on Foundations of Computer Science (FOCS).
Literature cited 2: Boyes, et al., 2001. The plant Cell.1499-1510. Clough, S.J. and Bent, A.F. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16 (6): 735-743. Doi: 10.1046/j.1365-313x.1998.00343.x.PMID 10069079.

ID: 59927
Title: Isolation and production of biosurfactant from marine Bacteria.
Author: A. Jayachitra, A. Soma Prabha, T.S. Blessy Thayalin and V. Prabakaran.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 819-825 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Biosurfactants, Bacillus Sp, Emulsification, Hemolysis, Oil displacement.
Abstract: Surfactants, surface active amphiphilic molecules, have a broad spectrum of applications, from everyday tasks such as washing the dishes to advanced oil recovery operations. Biosurfactants are amphiphilic compounds which are produced on living surfaces, mainly on surfaces of microorganisms or may be also secreted extra cellularly and it contains both hydrophilic and hydrophobic moieties which reduces the surface and interfacial tension of the surface and interface respectively. The present study demonstrated the isolation and characterization of biosurfactant producing microorganisms from sea water and sediment samples. The screening of biosurfactant producing microorganisms was carried out employing different parameters such as surface activity and emulsification activity. Our study revealed strain S2 and S3 showed positive results. Oil displacement test and Haemolytic activity revealed an enhanced activity. On analysis with emulsification activity with petrol as substrate it was found out that an increased emulsion with 72 % was recorded against Bacillus subtilis (S3) and followed by (S2) Bacillus cereus. Besides, on carbon sources tested as glucose, sucrose, petrol exhibited efficient biosurfactant production. An optimum pH of 8 was found better for surfactant production. The crude surfactant production from Bacillus obtained from marine samples were tested for antibacterial activity against pathogens Klebsiella pneumonia, Pseudomonas aeruginosa, Pseudomonas fluorescence and E.coli were found effective in inhibition of pathogens.
Location: TE 15 New Biology Building
Literature cited 1: Benincasa, M., Contiero, J., Manresa, M.A. and Morase, J.O. 2002. Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soap stock as the sale carbon sources. Journal of food Engineering. 54: 283-288. Cooper, D.G. and Goldenberg, B.G. 1987. Surface-active agents from two Bacillus species. Appl Environ Microbiol. 53 (2): 224-229.
Literature cited 2: Desai, J.D. and Banat, I.M. 1997. Microbial production of surfactants and their commercial potential. Microbiology and Molecular Biology Reviews. 61 (1): 47-64. Haddad, N.L.A., Wang, J.and Mu, B. 2009. Identification of a surfactant producing strain: Bacillus subtilis HOB2.Prot Pept Lett.16: 7-13.

ID: 59926
Title: Biodegradation of complex hydrocarbon compounds by soil isolates of Dehradun region, India.
Author: Kunal Madhav, Neha Tanwar, Nupur Gusain, Latika Sharma and Aditya Chaudhary.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 811-817 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Naphthalene, Biodegradation, Paracocuus spp., Paenibacillus spp.
Abstract: PAH like naphthalene are so toxic there is interest in understanding the physicochemical processes and microbial degradation reactions that affect the mobility and fate of these compounds in groundwater and soil sediment system. The present work focuses on Biodegradation of petroleum Hydrocarbons by bacterial soil isolates of soil samples were collected in sterile containers from petrol pumps. The soil sample from selected sites were diluted and plated on sterile NAM plates. Colonies of Bacillus spp., Klebsiella spp., Paracoccus spp. and Paenibacillus spp. were obtained. The isolates obtained were then tested against Petrol, Diesel, Lubricating oil and Naphthalene which were incorporated in Basalt Salt Media (broth and agar) and were incubated at 30 ? C for five days. Readings from the broth culture was recorded every 24 hours at 595 nm for five days. Readings from the broth culture was recorded every hours at 595 nm for five days and the results were depicted graphically. The results obtained were compared with hydrocarbon utilizing Pseudomonas aeruginosa MTCC 2474 strain.
Location: TE 15 New Biology Building
Literature cited 1: Abd-Elasalam, E. Hassan, Hafez, E. Elsayed, Hussain, A. Azhar, Ali G. Amnay and El-Hanafy, Amr, 2009. Isolation and identification of three-rings polyaromatic hydrocarbons (anthracene and phenanthracene) degrading bacteria. American Journal of Agriculture and Environmental Science5 (1): 31-33. Alquati Claudia, Papacchini Maddalena, Riccardi Carmela, Spicagelea Sergio and Besttteti Giuseppinea, 2005. Diversity of Napthelene-degrading bacteria from a petroleum contaminated soil. Annuals of Microbiology. 55 (4): 237-242.
Literature cited 2: Bist Sandeep, Pandey Piyush, Sood Anchal, Sharma Shivesh and Bisht, N.S. 2010. Biodegradation of Napthelene and anthracene by chemo-tachtically rhizobacteria of Populus Deltoids. Brazalian Journal of Microbiology. 41: 922-930. Chaudhary Kumar Vivek and Borach Debajit. (2011).Isolation and molecular characterization of hydrocarbon degrading bacteria from tannery effluent. International Journal of Plant, Animal and Environmental Science. 1 (1): 2231-4490.

ID: 59925
Title: Influence of fym and VAM on yield, nutrient content and antioxidant activity of spinach (Beta vulgaris) grown under water stress conditions.
Author: Tirupathi Rao H., S. Sumathi, R, Subhash Reddy and S. Padmaja.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 805-809 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Water stress conditions, Farm yard manure, Mycorrhizae, Nutrient content and antioxidant activity.
Abstract: To study the effect of FYM and VAM on yield, nutrient content and antioxidant activity of spinach under drought conditions. Recommended dose of chemical fertilizers (100 % RDF, 50 % RDF), farm yard manure and mycorrhizae were applied to the soil according to the treatments imposed. All green variety of spinach seeds (20) were sown in polybags and watered at every 4th day to impose drought condition. The crop was harvested around 40 days after sowing. The results revealed that significantly higher the crop was harvested around 40 days after sowing. The results revealed that significantly higher yield was found in 100 % RDF +FYM+VAM followed by 50 % RDF +FYM + VAM followed by 100 % RDF. Highest chlorophyll content was recorded in 50% RDF + FYM + VAM followed by 100 % RDF + FYM VAM. Highest chlorophyll content was recorded in 50 % RDF +FYM + VAM followed by 100 % RDF +FYM+ VAM. Highest antioxidant activity recorded in 100% RDF +FYM +VAM followed by 100 % RDF and VAM. Highest vitamin C content was recorded in both 100% RDF +FYM +VAM and 50 % RDF +FYM+ VAM. Highest carotene content was recorded in FYM with 100 % RDF followed by 100 % RDF + FYM +VAM and 100 % RDF. Significantly highest phenolic content found in 100 % RDF +FYM + VAM followed by 50 % RDF +FYM +VAM. Highest nitrogen uptake was recorded in 50 % RDF +FYM +VAM followed by 100 % RDF +FYM +VAM. Highest phosphorus uptake was recorded in 100 % RDF+FYM + VAM. Highest phosphorus uptake was recorded in 100 % RDF +FYM +VAM. Lowest uptake recorded in VAM and it was on par with the treatments 100 % RDF, FYM, FYM+VAM.
Location: TE 15 New Biology Building
Literature cited 1: Bolan, N.S. 1991. A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. Plant and Soil. 134: 189-207. Bhosale, K.S. and Shinde, B.P. 2011. Influence of arbuscular Mycorrhizal fungi on proline and chlorophyll contents in Zingiber officinale rose grown under water stress. Indian Journal of Fundamental and Applied Life Sciences. 1 (3): 172-176.
Literature cited 2: Bvoungyeul, R., Jeonasik, L., Ryu, B.Y and Lee, J.S. 1996. Property changes in mixed media for pot flower made of several organic materials. Journal of the Korean Society for Horticultural Science. 37: 127-135. Farahani, H.A and Khavati, M.A. 2011. Effects of arbuscularmycorrhizal fungi and phosphorus on coriander (Coriandrum sativum L.) essential oil in drought stress condition. 46th Croatian & 6th International Symposium on Agriculture.

ID: 59924
Title: Screening of Coriander genotypes for powdery mildew disease resistance.
Author: Bandela sravanthi, B.S. Sreeramu, B. Narsimha swamy and K. Umesha
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 803-804 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Coriander, Powdery mildew.
Abstract: Twenty five genotypes of coriander were evaluated at Post Graduate Centre, UHS Campus, GKVK, Bangalore to screen the powdery mildew disease resistance, caused by Erisyphe spp which is a serious disease. Among the genotypes, seventeen genotypes were highly susceptible, eight genotypes were susceptible and none of them showed resistance reaction.
Location: TE 15 New Biology Building
Literature cited 1: Kalra, A. Gupta, A.KKatiyar, N. Srivastava, R.K. and Kumar, S. 2003. Screening of Coriandrum sativum accessions for seed and essential oil yield and early maturity. Plant Genetic Resources Newsletter, 133: 19-21. Kalra, A. Parameswaran, T.N. Ravindra, N.S and Dimri, B.P. 1995. Effect of number of timing of application of dinocap on control of powdery mildew and yield of coriander cultivars .J. Agric. Sci 79 (1): 7-11.
Literature cited 2: Keshwal, R.L. and Khatri, R.K. 1998. Reaction of some high yielding varieties of coriander to powdery mildew.J.Mycology & Plant Path. 28 (1): 58-59.

ID: 59923
Title: Biocompatible formulation of potential fungal biopesticide Nomuraea Rileyi (F) samson for the improved post treatment persistence and biocontrol potential.
Author: S. Karthick Raja Namasivayam, Asbinaya Vidyasankar and T. Manoj Santhosh.S. Karthick Raja Namasivayam, Asbinaya Vidyasankar and T. Manoj Santhosh.S. Karthick Raja Namasivayam, Asbinaya Vidyasankar and T. Manoj Santhosh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 797-801 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Nomurae rileyi, Formulation, persistence, Biogel, Spodoptera litura.
Abstract: The development of pest control measures using microorganisms especially entomopathogens has received increasing attention in recent years. Formulation of biological control agent is an important criterion for sustainable agriculture. Fungal biopesticide mainly Nomuraea rileyi is widely used to control various economic important insect pests. In the present study, various formulation of N.rileyi biogel, oil and hydro gel was prepared and the prepared respective formulation was evaluated for post treatment persistence under different temperature and the biocontrol potential against groundnut defoliator Spodoptera litura (Fab) (Lepidoptera; Noctuidae). Among the formulation, maximum rate of persistence was recorded in biogel. Fungal spore could retain the viability in all the tested temperature in biogel formulation. Enhanced pesticidal activity was also recorded in the same formulation. The present study suggests the possible utilization of biogel formulation of N. rileyi to control economic important pests under field condition.
Location: TE 15 New Biology Building
Literature cited 1: Alter, J.A. and Vandenberg, J.J.D. 2000. Factors that influencing the infectivity of isolates of Paecilomyces fumosoroseus against diamond back moth.J.Inverterbr Pathol. 78: 31-36. Asensio, A., Carbonell, T., Jimenez, L., Liorca, L. 2003. Enntomopathogenic fungi in soils from Alicants province. Spanish Journal of Agriculture Research.1: 37-45
Literature cited 2: Brar, K., Dhiruga, K. and Kaul, N. 2004. The influence of sowing and harvesting date of the yield and yield attributes of four groundnut genotype planted during summer.Indian J. Environ. Ecop. 12: 185-188. Clark, M. 1997. Microorganisms Standard Isolation Techniques, Academic Press, London. P 271-275.

ID: 59922
Title: A study on the swelling characteristics of expansive subgrade soil treated with different chloride compounds.
Author: G.Radhakrishnan, M. Anjan Kumar and G.V.R. Prasada Raju
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 793-796 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Expansive soil, Swell pressure, Chloride Compound Chemicals.
Abstract: The swell shrink behavior of expansive soils is creating a major challenge to the Geotechnical engineers and is posing a problem to many National Highways constructed on these soils. The swelling pressures developed on the pavement system laid on these soils will severely damage the pavement performance. Among various controlling techniques chemical stabilization of expansive soils has gained prominence due to its easy applicability and adaptability. A laboratory testing is carried out to investigate the effect of chloride compound chemicals on the swelling and strength characteristics of expansive soil. The swelling properties of the collected sample are determined based on the Free Swell Index, Swell Potential and Swell pressure tests. Test results have shown that the collected sample fell under the category of high swelling. In this present study Chloride Compound Chemicals like Ammonium Chloride (NH4Cl), Magnesium Chloride (MgCl2), and Aluminum Chloride (AlCl3) were selected to use for reducing the swelling potential of the expansive soil. The testing results indicate that the swelling pressure is reduced considerably with the increase in addition of chemicals and remained constant after reaching certain chemical concentration. This paper discusses the results of the laboratory testing.
Location: TE 15 New Biology Building
Literature cited 1: Bara, J.P.1969. Controlling the expansion of desiccated clays during construction. Proc. of 2nd Int. Research and Engg.Conf.on Expansive Clay Soils, Texas A& M Press, Texas, PP. 380-390 Chen, F.H. 1988. Expansive soils-whatt have we accomplished. Proc. Of Int.Conf.on Engg.Problems of Regional Soils, Beijing, China, pp.561-563.
Literature cited 2: Evans, R.P. and Mc Manus, K.J. 1999. Construction of vertical moisture barriers to reduce expansive soil subgrade movement.TRR-1652, TRB, PP. 108-112. Gromko, G.J. 1974. Review of expansive soils. Journal of the Geotechnical Engg.Division , Proc. Of ASCE. 100 (GT6): 667-687.

ID: 59921
Title: Occurrence of Clostridium perfringens in raw meat, poultry and meat spices and effect of sodium chloride and reduced pH on the outgrowth of their spores.
Author: Vijay Singh Chhetri and Tika Bahadur Karki.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 787-791 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Clostridium perfringens, Buffalo meat, Chicken, Meat spices, NaCl, Lactic acid.
Abstract: The incidence of Clostridium perfringens and their spores was examined in raw meat and poultry, ready to eat meat items and meat spices, collected from the retail market of Kathmandu city for the first time. Among the examined samples, 80.80 % of chicken, 87.75 % of buffalo meat and 72 % of meat spices were positive for the spore. The highest spores count was 5.14 x 103 CFU/g in raw buffalo meat, 2.7x 103 CFU/g in chicken sample and 3.0 x 103 CFU/g in meat spices. In ready to eat products, 69.23 % of chhoyela, 88.23 % of sausages, 100 % of kachila samples were positive for vegetative cells, and count of 5 % samples was more than 106 CFU/g. The spore outgrowth was reduced by 0.36%, 1.1 %, 2.58 %, 4.0 %, 18.45 % and 37.26 % at sodium chloride concentration of 0.5 %, 1.0 %, 1.5 %, 2.0 %, 2.5 % and 3 % respectively. No outgrowth was observed at and above 3.5 % sodium chloride. The rate of spore outgrowth reduced with declining pH. At pH of 5.2 and below, no outgrowth was observed up to 48 hours.
Location: TE 15 New Biology Building
Literature cited 1: Adak, G.K., Long, S.M. and O ' Brien, S.J. 2002. Trends in indigenous food borne disease and deaths, England and Wales: 1992 to 2000.Gut. 51: 832-841. Banerjee, M. and, Sarkar P.K. 2003.Microbiological quality of some retail spices in India. Federal Register.36: 469-474.
Literature cited 2: Behling, R.G., Eifert, J., Erickson, M.C., Gurtler J.B., Kornacki, J.L., Line, E., Radcliff, R., Ryser, E.T., Stawick, B. and Yan,Z.2010.Selected Pathogens of Concern to Industrial Food Processors:Infectious, Toxigenic,Toxico-Infectious,Selected Emerging Pathogenic Baacteria.In: Kornacki J.L. (ed). Principles of Microbiological Troubleshooting in the Industrial Food Processing Environment, Food Microbiology and Food Safety, Springer Science + business Media, LLC, pp. 5-61. Borriello, S.P. 1995. Clostridial disease of the gut. Clinical Infectious Diseases.

ID: 59920
Title: Isolation and characterization of Bacterial Endophytes associated with sago palm (Metroxylon Sagu Rottb) in Tissue culture.
Author: K.L.Labrador, E.L.T.Lustica and A.U. Novero
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 777-786 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Sago palm, Metroxylonsagu, Endophytes, Pathogen inhibition.
Abstract: Endophytic bacteria are novel sources and beneficial compounds such as plant growth regulators and antibiotics. Nine bacterial isolates were obtained from sago palm tissue culture. Selected candidates were those colonies observed to have originated from the base of the explants. Eight of the isolates were Gram-negative bacilli (SEB 001, 002, 003, 004, 005, 006, 007, and 008) and one (SEB 206) was Gram-positive in catalase, citrate and VP tests. The isolates were negative for endospore formation, indole production, and methyl red test. Only one isolate grew in mannitol salt agar. Amplification of 16s rDNA sequences using the universal primers 16F27 and 16R1542 identified the isolates as Enterobacter ludwiggi (SEBs 001, 003, and 006), Pantoea dispersa (SEBs 002 and 004), and Klebsiella variicola (SEBs 005, 007, and 008). Morphological and biochemical properties of the test isolates, as well as phylogenetic analysis, support the deduced identities. All isolates inhibited the growth of E.coli in the disc diffusion assays with SB 006 obtaining the highest mean zone of inhibition (12 mm). In the resazurin test, SEB 007 and SEB 008 showed promising antibiotic activities against E.coli, Aspergillus niger and fusarium oxysporum. This study revealed that E. ludwigii and Kvariicola isolated from sago palm possess antibiotic properties.
Location: TE 15 New Biology Building
Literature cited 1: Acu Media.2011.Mannitol salt agar (7143) Neogen Corporation. USA.1-2. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J.Z.Z., Miller, W., and Lipman, D.J. 1997. Gapped BLAST AND PSI-BLAST: A new generation of protein database search programs.Nucleic Acids Res.25: 3389-3402.
Literature cited 2: Bai, Y., Zhou, X. and Smith, D.2003.Enhanced soybean plant growth resulting from coinocculation of Bacillus strains with Brodyrhizobium japonicum.Crop Sci.43: 1774-1781. Becton, Dickinson (BD) and Company. 2007. BBL Mannitol salt agar quality control procedures. Maryland, USA.1-2.

ID: 59919
Title: Isolation of Halotolerant Rhizobacteria from saline soil and their effect on rice seed germination.
Author: Pornrapee Sarin, Sophon Boonlue and Nuntavun Riddech.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 767-776 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Plant growth promoting halotolerant rhizobacteria, Rice rhizosphere, Saline soil, Seed germination.
Abstract: Halotolerant rhizobacteria were isolated from rice rhizosphere samples in saline in Khon Kaen and Mahasarakam provinces, Thailand.Among 3 isolates, P8, N15 and 12.1, the isolates P8 and N15 were able to fix nitrogen from the air and to solubilize tri-calcium phosphate on the National Botanical Research Institute ' s phosphate growth medium with the solubilization index of 2.42 and 2.93, respectively. The isolate 12.1 produced indole-3-acetic acid (IAA). Among 3 isolates, N15 showed the highest cellulose degradation activity on the carboxymethyl cellulose agar (CMC agar) plate with the maximum hydrolysis capacity value of 5.49. The isolate 12.1 showed the potential of inhibition the growth of Pyricularia oryzae, a fungal pathogen of rice blast disease with the percent inhibition of radial growth (% PIRG) of 42.4 %.Mixculture of rhizobacterial isolates P8, N15 and 12.1 were used for stimulating rice seed germination in various concentrations of NaCl solution and the germination index of rice seed was greater than 80 % in 5-10 dS/m of NaCl solution and the germination index of rice seed was greater than 80% in 5-10 dS/m of NaCl solution. Using 16S rDNA sequence analysis, three halotolerant rhizobacteria isolates, 12.1, P8 and N15, were identified as Pseudomonas azotoformans, Enterobacter aerogenes and Bacillus tequilensis, respectively.
Location: TE 15 New Biology Building
Literature cited 1: Altschul, S.F., Madden, T.L., Schaeffer, A.A., Zhang, J., Milller, W. and Lipman, D.J. 1997.Gappecd BLAST and PSI-BLAST: a new generation of protein database search program. Nycleic acids Res.25: 3389-3402. Boddey, R.M.1995.Biological nitrogen fixation associated with sugar cane and rice: contributions and prospects for improvement. Plant Soil.174: 195-209.
Literature cited 2: Chookietwattana, K. and Maneewan, K.2012. Screening of efficient halotolerant phosphate solubilizing bacterium and its effect on promoting plant growth under saline conditions. World Appl Sciences J.16 (8): 1110-1117. Clermont-Dauphina, C., Suwannangb, N., Grunbergerc, O., Hammeckera, C. and Maeght, J.L.2010.Yield of rice under water and soil salinity risks in farmers ' fields in northeast Thailand. Field Crop Res. 118: 289-296

ID: 59918
Title: PRODUCTION OF CELLULASE FROM BACILLUS SPECIES USING CELLULOSE FROM BREWERS SPENT GRAIN (BSG) AS SOLE CARBON SOURCE.
Author: Orji, F.A., Dike, E.N., Lawal, A.K., Elemo, G.N., Olatope, S.O.A., Famotemi, A.C., Itoandon, E.E., Suberu, Y.L., A Sieba, G.O., Sadiq, A.O., Olasore, O., Okafor, E.N., Ugbana, A.I. and Adefiranye, A.O.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 839-848 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Cellulase, Brewers Spent Grains (BSG), Fermentation and waste utilization.
Abstract: Cellulose from Brewers Spent Grain (BSG) and crystalline cellulose were utilized as sole carbon source for the submerged production of Cellulase. A total of 21 bacterial isolates were obtained from brewers Spent Grain undergoing deterioration. Out of the 21 isolates, 7 bacterial isolates showed various degrees of Cellulase production on plate assay (Nutrient Agar + 2 % (W/V) Cellulose) by their zones of clearance of Cellulose. The Brewers Spent Grain was characterized for its composition, and its moisture content, Crude Fibre, Fat, Protein, Ash and Total carbohydrate were 17.62 %, 13.94 %, 8.08 %, 25 %, 3.68 % respectively. Cellulase production screening of bacterial isolates showed that Bacillus species strain B223 had the highest zone of clearance (2.8 cm ? 0.02), and was used for the submerged Cellulase production. Microbial population dynamics in the enzyme production medium in the 250 ml capacity Erlenmeyer flasks containing BSG cellulose increased progressively from zero hour to the 120 hour. pH of the BSG cellulose medium increased from 5.28 to 6.89, while the set-up containing crystalline cellulose had its pH increased from 5.28 to 7.46. Crude cellulose activity of the cellulose in Filter paper Units (FPU) for the cellulose produced from BSG Cellullose ranged from 328 FPU, 2749 PU, 32166 FPU, and 33130 FPU at zero hour, 24 hour, 72 hour, 96 hour and 120 hour of fermentation respectively. The optimum pH for the production of Cellulase was 6.89. Specific Cellulase activities of Cellulase produced using BSG cellulose and Crystalline cellulose were between 12.45-4319.4 (Unit/Mg) and 19.6-5711 (Unit/Mg) respectively. Cellulases are industrial enzymes with broad applications, and production using raw materials like cellulose from Brewers Spent Grain makes the enzyme cost effective, and converts the waste (BSG) to wealth. Further research actions are on-going to purify the crude Cellulase.
Location: TE 15 New Biology Building
Literature cited 1: Akinola, S.O., Kuboye, A.O. and Olatuji, O. 2004. Use of fungal pectinase for clarification of Fuava juice.Nigerian Journal of Microbiology. 18 (1-2): 293-296. Ariffin, H., Abdullah, N., Umi Kalsom, M.S., Shirai, Y. and Hassan, M.A. 2006. Production and characterization of cellulose by Bacillus pumilus EB. International Journal of Engineering and Technology. 3 (1): 47-53.
Literature cited 2: Awojobi, K., Olutiola, P., Agboola, F. and Arrotupin, D. 2012. The screening and Selection of Trichoderma species capable producing extracellular cellulolytic Enzymes from Sol of decaying plant materials, Book of Abstract, Conference of the Nigerian Society of Microbiology held at Nnamdi Azikiwe university, Awka, Nigeria. Pp 139. Bai, S., Ravi kumar, M., Mukesh Kumar, D.J., Balashanmugam, P., Bala Kumaran, M.D. and Kalaichelvan, P.T. 2012. Cellulase Production by Bacillus subtilis isolated from Cow Dung. Archives of Applied Science Research. 24 (1): 269-279.