Enter records

ID: 59947
Title: Comparative study on pollution potential of Dairy wastewater generated by organized and unorganized sector.
Author: Seema Garcha, N.Verma and S.K.Brar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 943-948 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Dairy wastewater, Organized sector, unorganized sector, Statistical analysis, Pollution parameters, BOD.
Abstract: The aim of the present investigation was to document characteristics exhibited by the dairy wastewater generated by the organized and unorganized sector. Thirty samples were collected from each sector and analyzed for various pollution parameters as per standard procedures. The analysis of various parameters showed that the effluent generated from unorganized sector is richer in organic content as their C:N ratio (37.6) was over three times the C:N ratio for organized sector wastes (11.9). pH of dairy waste water generated by both the sectors varied between 3.0 to 8.3. 90 % of the samples drawn from unorganized sector were markedly acidic compared to only 56.6 % samples from the organized sector. In the unorganized sector average oil and grease content of samples was recorded as 1792.67 mg/L and for organized sector it was recorded as 919.33 mg/L. Total suspended solids ranged between 1000-15000 mg/L for unorganized sector and 500-3000 mg/L for organized sector. Average BOD3 of samples collected from organized sector which was 2196.61 mg/L. The results were analyzed statistically significant at 95 % confidence interval. There is limited information available about the characteristics of the effluent of the unorganized sector. Significant variations between major pollution parameters warrants different treatment approaches for effluents generated by these two sectors.
Location: TE 15 New Biology Building
Literature cited 1: Akpor, O.B. 2011. Wastewater effluent discharge: effects and treatment processes. IPCBEE.20: 85-91. Amudha, P., Nagendran, R.and Mahlingam, S. 1997.
Literature cited 2: Christian, O. 2010. Characteristics of untreated wastewater produced by food industry.Anlele Universatii din Oradea, Fascicula: Protectia Mediului.15: 709-714. Dhanan, S. 2009. Effect of dairy effluent on seed germination, seedling growth and biochemical parameters in paddy. Botany Res Intl. 2: 61-63.

ID: 59946
Title: A quantitative study on the effect of ' Hot Water Treatment ' on Pseudomonasa Biofilm using Atomic Force Microscopy.
Author: G.Panneerselvam, Ashwin Kannan and Pennathur Gautam.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 941-942 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pseudomonas aeruginosa, Biofilm, Adhesion force, Adhesiveness.
Abstract: In this study, the effect of hot water treatment on the adhesion force of Pseudomonas aeruginosa (MTCC2297) biofilm was studied using Atomic Force Microscopy. The adhesion force of four day old biofilm decreased from 11.6 ? 0.2nN after incubation in hot water for one day at 50?C. The adhesion force further decreased to 2.1? 0.1 nN after incubation in hot water for two days at 50?C. The results suggest that the adhesion force of biofilm may be reduced by incubating biofilm sample in hot water.
Location: TE 15 New Biology Building
Literature cited 1: Ashwin, K., Ramalingam, S.and Gautam, P.2014.A study on Pseudomoinas aeruginosa biofilm formation using Atomic Force Microscopy.Asian Jr. of Micobiol.Biotech.Env.Sci. 16 (2): 145-146. Parsek, M.R. and Singh, P.K. 2003. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 57: 677-701.
Literature cited 2: Quraishi, M.A., Kumar, V., Singh, B.N. and Singh, S.K. 2012. Calcium palmitate: A green corrosion inhibitor for steel in concrete environment. J Mater and Environ Sci. 1001-1008. Zelaya, A.J., Stough, G., Rad, N., Vandervoort, K. and Brelles-Marino, G. 2010. Pseudomonas aeruginosa biofilm inactivation: decreased cell culturability, adhesiveness to surfaces and biofilm thickness upon high-pressure nonthermal plasma treatment. IEEE Transactions on Plasma Sci. 38: 3398-3403.

ID: 59945
Title: Proteomic profiling of Brevundimonas sp.MJ 15 on exposure of Imidacloprid
Author: A.A.Shetti and B.B.Kaliwal.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 935-940 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Proteomic, Imidacloprid, Brevundimonas Sp.Mj 15, Stress Proteins.
Abstract: Imidacloprid [1-(6-chloro-3-pyridylmethyl)-2nitroimino-imidazolidine] has gained great attention as a synthetic insecticide that acts in a similar manner as nicotine and is now widely used for the control of pests. Microorganisms respond to environmental or chemical stress by synthesizing specific sets of proteins that can be analyzed by protein profiling using SDS PAGE. The present investigation was undertaken to elucidate protein profile changes and differently expressed proteins in soil isolate Brevundimonas Sp.MJ 15 on dose and durational exposure to imidacloprid. Protein profiling of Brevundimonas Sp.MJ 15 treated with different (10-7 to 10-3 M) concentrations of imidacloprid observed regularly at the dutration of 24 hrs for a period of 96 hours, showed the over expressions of certain protein, expression of stress proteins and novel proteins. The proteins induced were observed to be dose and durational dependent. The present investigation proves that imidacloprid exposure induces the expression of stress and novel proteins in soil isolate Brevundimonas Sp.MJ 15 from toxic effects of exposure to imidacloprid. The specific protein profiles that are expressed in response to the stress induced by imidacloprid can be used to monitor the environmental samples for the presence of similar pollutants.
Location: TE 15 New Biology Building
Literature cited 1: Araya, T., Ishibashi, N., Shimamura, S., Tanaka, K. and Takahashi, H. 1993. Genetic and molecular analysis of the rpoD gene from Lactococcus lactis. Biosci.Biotechnol.Biochem.57; 88-92. Asghar, M.N., Ashfaq, M., Ahmad, Z., Khan, I.U. 2006. 2-D PAGE analysis of pesticide induced stress proteins of ER.coli Anal Bioanal Chem. 384: 946-950.
Literature cited 2: Bell, D.Y. and Hook, G.E. 1979.Pulmonary alveolar proteinosis: analysis of airway and alveolar proteins.Am Rev Respir Dis.119: 979-990. Bhargava, P., Yogesh, M., Ashish Kumar, S., Anjum, A. and LalChand, R.2006. Preliminary analysis of cuprome of anabaena doliolum using two dimensional gel electrophoresis.Curr.Sci.91: 1521-152.

ID: 59944
Title: Quality characterization of market available Tungtap-A fermented fish product of Meghalaya, India.
Author: M.Bhargavi Priyadarshini, Deepayan Roy, R.K.Majumdar and Subbaih Kanasi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 931-934 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Tungtap, Fermented fish, Khasi tribes, Danio sp, Puntius sp.
Abstract: Fermented fish products are popular in the North eastern parts of India because of their characteristics flavour and aroma. Tungtap is a fermented fish product prepared from Puntius and/or Danio spp. And consumed as pickle and taste enhancer by the khasi tribes of Meghalaya.The study was carried out to assess the quality parameters of market available Tungtap. The results of the analysis showed that the market sample of Tungtap was a good source of protein, lipid and ash, i.e.37.05%, 15.33% and 15.35% respectively but the biochemical parameters were found to be relatively higher. The microbial numbers were also relatively higher and the presence of Vibrio and E.coli indicates unhygienic processing or retailing. There were no visible fungal colonies on the product; however when grown on selective medium, a few fungal colonies were observed. But the product was safe from human consumption point of view.
Location: TE 15 New Biology Building
Literature cited 1: Abrami, G., Natiello, F., Bronzi, P., Mckenzie, D., Biols, L. and Agradi, E. 1992. A comparison of highly unsaturated fatty acids levels in wild and farmed eels (Anguilla anguilla). Comp.Biochem. & Physiol. 101: 79-81. Antonacopoulos, N. and Vyncke, W. 1989. Determination of volatile basic nitrogen in fish: a third collaborative study by West European Fish Technologiests ' Association (WEFTA). Zeitschrift fur Lebensmittel-Untersuchung und-Forschung A.189: 309-316.
Literature cited 2: Anihouvi, V.B., Ayernor, G.S., Hounhouigan, J.D.and Sakyi-Dawson, E.2006.Quality characteristics of Lanhouin: a traditionally processed fermented fish product in the Republic of Benin.Afri.J.Food Agri.Nutri & Dev. 6(1): 1-15 AOAC, 2005. Official Methods of Analysis, Association of Official Analytical Chemists International, 18th Edition.In: Horwitz, W. (Ed). AOAC, Washington (D.C) 35: 2-36.

ID: 59943
Title: Effect of different sowing dates on the growth behaviour of different varieties of Black Gram.
Author: Arvind Kumar and Braj Kishor.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 925-929 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Sowing time, Black gram, Performance, Yield.
Abstract: Present paper deals with effect f different varieties of black gram viz.PDU-1, TAV-1 and UG-201. June sown plants. The poor performance of August and September sown plants was reflective of the non-adjustability in the climatic conditions of these months. TAV-1 variety appeared to be the best performer in all the four treatments. Introduction of PDU-1 for better pod yield while TAV-1 and UG-201 for better forage may be advocated.
Location: TE 15 New Biology Building
Literature cited 1: Anderson, L.R. and Vasilas, B.L1985. Effect of planting date on two soybean (Glycine max) cultivars: Seasonal dry matter accumulation and seed yield.Crop.Sci. 25 (6): 999-1004. Briggs, C.E. et al. 1920. A quantitative analysis of plant growth I and II. Ann.Appl.Biol.7: 103-202.
Literature cited 2: Briggs, C.E. et al. 1920. A quantitative analysis of plant growth I and II. Ann.Appl.Biol.7: 103-202. Evans, G.C. 1972.Quantitative analysis of plant growth.Blackwell, England.

ID: 59942
Title: Effect of fertigation scheduling on yield and quality of sweet orange (Citrus sinensis L) Under Nalgonda District, A.P, India.
Author: G. Lakhpathi, D. Uma Maheshwra Rao and P. Rajanikanth.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 921-924 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Sweet orange, Fertigation, Yield, Quality parameters.
Abstract: An investigation was carried out to study the effect of fertigation on 14 years sweet orange in Ambe Bahar production season at Velmagudem and Annaram villages of Nalgonda. Fetilizer application through drip system to 14 years old sweet orange orchard produced favourable effect on minimum number of day to flowering (25 days), more number of fruits per tree (815), fruit diameter (30.17 cm) and average fruit weight (176.78 g).It is shown significant variations as productivity was increased from 79.42 kg to 144.14 kg per tree and also improved fruit quality parameters viz., TSS increase from 7.59 to 9.96? Brix (? B= Degree Brix) and acidity decrease from 1.03 to 0.80 per cent.
Location: TE 15 New Biology Building
Literature cited 1: Burt, C.K. O ' Connor and Rhuer, T. 1988. Fertigation. Irrigation training and research Center. California Polytechnic State University, San Luis Obispo, CA.p.295. Dinesh Kumar, Pander, V. and Vishal Nath, 2012. Growth, yield and quality of vegetable banana Monthan (Banthal-ABB) in relation to NPK fertigation. Indian J. Hort. 69 (4): 467-471.
Literature cited 2: Glenn, D.M. 2000. Physiological effects of incomplete rot-zonewetting on plant growth and their implications for irrigation management. Proceedings of the Colloquim ' Water management and water relations of horticulture crops ' , USA.Hortsc. 35:1041-1043. Kausadikar, H.K. 2006. Studies on nutritional disorders in sweet orange and their response to multi-micronutrient application.Ph.D. Thesis, submitted to Marathwada Agricultural University, Parbhani.

ID: 59941
Title: Study of Myco-Ecological diversity with special reference to historical monuments.
Author: Neha Sharma, Shraddha Dubey, Rita Singh and D.S. Rathore.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 917-920 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Biodeterioration, Fungal spp. Monuments, Pigments, Organic acid.
Abstract: Cultural heritage assets are exposed to weather and submitted to influence of environmental parameters. Physicals, chemicals and biological factors interact with constitutive materials. The air contains a large amount of biological and a biotic component such as, pollen grains, fungal spores, insects, mites, fibers and dust particles but their number and concentration depend upon the geographical location, types of vegetation and meterological parameters. Fungal ability in producing pigments and organic acids have a crucial role in the discoloration and degradation of different types of stone in cultural heritage objects. Fungi are heterotrophic complex communities. They need organic matter for metabolism, thus produces pigments and organic acids. These acids are responsible for biodeterioration of various cultural objects. Additionally, stone objects may support the communities of microorganisms that are active in the biodeterioration process. Therefore, the aim of this paper is to summarize data on the fungal impact on biodeteroration processes. Present investigation focuses on mycobial survey of the Kamala raja girls college, Gwalior (M.P) and study carried out August 2013 to December 2014. During the investigation period 17 fungal species were isolated from the surface of monument. The fungal species isolated during the study are Aspergillus spp., Fusarium spp., Curvularia spp., Penicillium spp., Rhizopus spp., Rhodotorula spp., Candida spp. The most frequently isolated fungi belong to genus Aspergillus.
Location: TE 15 New Biology Building
Literature cited 1: Boyle, J.R. and Voight, G.K. 1973. Plant and Soil. 38: 191-201. Gupta, S.P. Sharma, K., Chhabra, B.S., Sharma, D.N. and Chandrol, G.K. 2012. Int.J.Curr. Res 4 (6): 045-047.
Literature cited 2: Lapidi, A.A. and Schipa, G. 1973. Some aspects of the growth of chemotrophic and heterotrophic bacteria from decayed stone. In: Proc. 5th Int. Congr. Deterior. Conserv. Stone. (Ed) G.Felix Lausanre, Switzerland. 633-640.

ID: 59940
Title: Virulence marker based multiplex PCR for detecting internalin C (INL C), Internalin J (INL J) and Listeriolysin o (Hlya) genes in Listeria spp.
Author: Samir Das, S.V.S. Malik, Sameer Shrivatasava, Pradeep Gandhale, Satish Kumar, Shabu Shoukat, Durga P. Das, S.B. Barbuddhe and D.B. Rawool.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 909-915 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Listeria monocytogenes, Internalin C, Listeriolysin O, Peptide and ELISA.
Abstract: A multiplex PCR (mPCR) targeting virulence gene (s) of Listeria spp. i.e, Internalin C (inlC), Internalin J (inlJ) and Listeriolysin O (hlyA) in case of Listeria monocytogenes and inl C in case of Listeria ivanovii was developed and evaluated on standard strains of Listeria spp. as well as isolates (25) of Listeria spp. with known biochemical and pathogenicity profiles based on other in vitro and in-vivo pathogenicity test with an ultimate view to evaluate this mPCR for raid and reliable identification and pathogenecity testing of listeria isolates was done. Out of many known virulence marker, listeriolysin-o encoded by hlyA gene is till date a well recognized one with few errors; here in this study InlC and inlJ genes were also studied in conjunction with hlyA to strengthen the existing hlyA marker. The mPCR showed presence of inlC gene in majority but not all virulent strains of pathogenic listeriae, however, it was also detected in some non-pathogenic L.monocytogenes strains. The same trend had been seen in, inlJ also, which revealed although these genes are linked to virulence but may not be an essential a full proof virulence marker for Listeria spp. However, its combination with other more specific virulence marker (s) need to be evaluated on known positive and negative Listeria strains with known pathogenecity profiles on a large number of Listeria isolates of various kind before drawing any final conclusion.
Location: TE 15 New Biology Building
Literature cited 1: Barbuddhe, S.B. and Malik, S.V.S. 2009. Recent advances in the molecular diagnosis of listeriosis. In: ICAR sponsored winter school on ?Molecular diagnostic techniques for zoonotic and foodborne infections?, Feb.07-27, Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, pp. 191-199. Bierne, H., Sabet, C., Personnic, N. and Cossart P. 2007. Internains: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes.Microbes Infect. 9 (10): 1156-1166.
Literature cited 2: Bikandi, J., San Millan, R., Rementeria, A. and Garaizar, J. 2004. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR and endonuclease restriction. Bioinformatics. 22; 20 (5): 798-799. Chakraborty, T., Hain, T. and Domann, E. 2000. Genome organization and the evolution of the virulence gene locus in Listeria species. Int. J.Med. Microbiol. 290: 167-174.

ID: 59939
Title: Inula Racemosa inhibits quorum sensing in Chromobacterium violaceum and Pseudomonas aeruginosa PA 01
Author: H.S. Vasavi, A.B. Arun and P.D. Rekha.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 903-907 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Inula racemosa, Chromobacterium violaceum, Quorum sensing, Virulence, Pseudomonas aeruginosa.
Abstract: Plants used in traditional medicienes are a promising source of bioactive compounds and some can potentially inhibit bacterial quorum sensing (QS ), a cell-cell signaling mechanism mediated by autoinducers to regulate a range of biological functions including virulence in bacteria. Inhibition of QS offers an alternative strategy for antibiotic mediated bactericidal/bacteristatic approach and believed to reduce the risk for development of resistance. The primary objective of this study was to investigate the anti-QS activity of inula racemosa Hook f., a member of the family asteraceae. Anti-QS activity of the ethanol extract of I. racemosa was determined by biosensor bioassay using Chromobacterium violaceum ATCC 12472 and swarming motility in Pseudomonas aeruginosa PA01 was also determined. The ethanol extract of I.racemosa showed anti-QS activity in biosensor bioassay as indicated by clear turbid halo zone of violacein inhibition in C. violaceum CV026 culture plates. It also showed inhibition of violacein production by more than 80 % in C. violaceum ATCC12472 at 1 mg/ mL concentration and complete inhibition of swarming motility in P.aeruginosa PA01 at 0.1 mg/mL concentration. Meanwhile, the growth of C. violaceum and P. aeruginosa PA01 was not affected significantly at all the tested concentration of I.racemosa ethanol extract. The ability of plants to produce substances that affect QS regulation provides with important tool to manipulate gene expression and control bacterial infections in humans.
Location: TE 15 New Biology Building
Literature cited 1: Changhong, L.Mishrta, A.K., Bing, H.E. and Renxiang, T. 2001. Antimicrobial activites of isoalantolactone, a major sesquiterpene lactone of inula racemosa. Chinese Sci Bulletin. 46: 498-504. Chong, Y.M., Yin, W.F., Ho, C.Y., Mustafa, M.R. Hadi, H.A. and Awang, K. 2010. Malabaricone C from Myristica cinnamomea exhibits anti-quorum sensing activity.J. Nat. Prod. 74: 2261-2264.
Literature cited 2: Choo, J.H., Rukayadi, Y. and Hwang, J.K. 2006. Inhibition of bacterial quorum sensing by vanilla extract. Lett.Appl.Microbiol. 42: 637-641. Cui, X. and Harling, R. 2005. N-acyl-homoserine lactone-mediated quorum sensing blockage, a novel strategy for attenuating pathogenicity of Gram-negative bacterial plant pathogens. European J. Plant Pathol. 111: 327-339.

ID: 59938
Title: Bioprocessing of an Eco-friendly microbial alginate and immobilization of an anticancer enzyme L-Glutaminase.
Author: E.SAM Jeeva Kumar, M.RaviKumar, Zahari Che Dan, S. Marylin Jeyapraya,
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 895-902 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Alginate, L-glutaminase, Immobilization, Biopolymer, Exopolysaccharide, Eco-friendly biopolymer.
Abstract: Seaweeds are the commercial source for the production of alginate, which I having a variety of applications. More precisely, they are used as viscosity enhances in food preparations and also in the field of biotechnology as good immobilizing substance for enzymes and proteins. The extraction of alginate causes considerable damage to ecology. The fish and marine life are interwoven to it in their habitat. Seaweeds are the bottom dweller of the food chain and they detoxify heavy metals such as cadmium and lead which will be a health concern if the products are manufactured out of it. Here we had isolated a soil dwelling microorganism found to be of Azobacter sp., which was potential enough to produce alginate. The parameters for the microbial production were optimized and the yield was significant after optimization. Temperature, pH, aeration and agitation of the fermentation medium were studied and optimized. The viscosity of the produced alginate of the fermentation medium were studied and optimized. The viscosity of the produced alginate was quiet appealing at all stages of the production. The strain yielded a maximum of 6.47 mg/mL of alginate. Microbial L-Glutaminase, an anticancer enzyme was selected for immobilization. The alginate produced was of good quality and the enzyme activity was steady for about 72 hours. The kinetics of the enzyme was studied using non-linear regression equation. Michelis Menten and lineweaver-burks plot were made. The enzyme Km and Vmax were found to be 459.2 mM and 1.383 mM/min respectively. The enzyme was found to have a high affinity towards its substrate.
Location: TE 15 New Biology Building
Literature cited 1: Adriana Navarro da Silva and Crispin Humberto Garcia-Cruz, 2010. Biopolymers by Azotobacter vinelandii. Department of Food Engineering and Technology, IBILCE/UNESP. Aravinth Vijay Jesuraj, S., Marylin Jeya Praya, S., BinoKingsley, R., Dinesh Kumar, L. and Ravikumar, M. 2013. Bioprocessing of AR isolates for economical production of L-Glutaminase by solid state fermentation. International Journal of ChemTech Research. 5 (4): 1428-1436.
Literature cited 2: Arruda, L.M.O and Vitolo, M. 1999. Characterization of invertase entrapped into calcium alginate beads. Applied Biochemistry and Biotechnology. 81: 23-34. Aquianti, L., Favilli, F. and Clementi, F. 2004. Comparison of different starategies for isolation and preliminary identification of Azotobacter from soil samples. Soil Biology & Biochemistry.

ID: 59937
Title: Susceptibility profile of bacteria isolated from diabetic wound to silver nanoparticles synthesized using Murraya Koenigii.
Author: S. Anantha Suganiya and R.Mala.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 887-894 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Diabetic wound, Multi drug resistance, Pseudomonas, Murraya koenigii, Silver nanoparticles.
Abstract: The major problem of wound management and therapy is bacterial infection and wound sepsis, which is worst case may, lead to mortality. Proper management of wound and successful wound healing requires the identity of colonizing bacteria and its sensitivity to antibiotics , as most of the pathogens have developed resistance to commonly used antibiotics. Hence, the present work is aimed to evaluate the prevalence, etiology and susceptibility pattern of bacterial pathogens isolated from diabetic wounds to commonly used antibiotics and silver nanoparticles. The sample was collected from Government Hospital in Aruppukkottai, Virudhunagar District, Tamilnadu, India. Silver Nanoparticles were synthesized using Murraya Koenigii. Out of 5 pathogenic isolates, predominant pathogen (3) was Pseudomonas aeurogenosa. Others were Enterobacter sp and Staphylococcus sp. Antimicrobial susceptibility test for isolates were executed using disk and well diffusion method. Pathogens were resistant to gentamycine, neomycine, cephalosporin, penicillin, metrogel, silvergel and ciprofloxacin. But sensitivity was intermediate to ciprofloxacin and susceptible to Silver Nano particles. Nano particles were characterized by XRD and FTIR. MIC of Silver nano particles (SNP) to the isolated P.aeruginosa ASM 100 was 1.5 mg/mL and MBC was 6mg/mL. So the present study reveals that Pseudomonas was highly resistant to most of the drugs used to treat infection. Susceptibility towards silver nano particles opens a window foe new wound dressing with SNP synthesized using edible leaf.
Location: TE 15 New Biology Building
Literature cited 1: Alexander, M.F. 1994. Wound Infection. In: Nursing Practice Hospital and Home, The Adult.Edited by Margaret F. Alexander, Josephine N.Fawcett, Phyllis J. Runciman.Pp.703, Churchill Livingstone, New York. Amro, N.A., Kotra, L.P., Wadu-Mesthrige, K., Bulychev, A.Mobashery, S. and Liu, G. 2000. High-resolution atomic force microscopy studies of the Escherichia coli outer membrane: structural basis for permeability.Langm. 16, 2789-2796.
Literature cited 2: Andrews, J. 2001. Determination of minimum inhibitory concentrations.J.Antimicrob.Chemother.48 (Suppl 1): 5-16 Baram-Pinto, D., Shukla, S.Perkas, N., Gedanken, A. and Sarid, R. 2009. Inhibition of herpes simplex vitrus type 1 infection by silver nanoparticles capped with mercaptoethane sulfonate.Bioconjug.Chem.20: 1497-1502.

ID: 59936
Title: Callus induction and plant regeneration from adventitious buds of ginger (Zingiber officinale Rosc.)
Author: Neeta Shivakumar, A. Pushpa and S. Laxmi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 881-885 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Humnabad, Zingiber officinale, Plant regeneration, Callus.
Abstract: Young buds of Zingiber officanale (Rosc) Cv. Humnabad (a variety grown in Northern Karnataka, India) were used for the initiation of callus growth on MS medium supplemented with various concentrations of NAA and BAP. The percentage of callus formation and average weight of callus were obtained from young buds cultured on medium supplemented with MS +1 mg/L NAA. Shoots were successfully regenerated on the medium with concentrations of MS + 1mg/L NAA+0.1 mg/1 BAP. The rooted shoots were successfully acclimatized in the green house conditions.
Location: TE 15 New Biology Building
Literature cited 1: Anwar, H., Lutful, H., Abdul, K.P., Sultan, M., Ahmad, S. D., Shah, A.H. and Fahrat, B. 2010. Establishment of a suitable and reproducible protocol for in vitro regeneration of ginger (Zingiber officinale Rosc). Pak.J.Bot. 42 (2): 1065-1074. Babu, K.N., Samsudeen, K. and Ratnambal, M.J. 1992. In vitro plant regeneration from leaf-derived callus in ginger. Plant Cell, Tiss Cul. 29: 71-74.
Literature cited 2: De Lange, J.H., Willers, P. and Nel, M. 1987. Elimination of nematodes from ginger (Zingiber officinale Rosc). By tissue culture. J. Horti. Sci., 62: 249-252. Geetha, S.P., Manjula, C., Jhon, C.Z., Minoo, D., Nirmal Babu, K. and Ravindran, P.N. 1977. Micropropagation of Kaempferia sp. (K. Galanga and K. R otunda). J. Spice Arom. Corps. 6 (2): 129-135.

ID: 59935
Title: Production of Alkaline protease enzymes from marine actinomycetes and its antimicrobial and anti-inflammatory studies using mice model.
Author: T.S. Blessy Thayalin and V. Prabakaran
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 875-879 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Alkaline protease, Anti-inflammation, Streptomyces, Antimicrobial activity.
Abstract: Actinomycetes are gram positive bacteria comprising a group of unicellular microorganisms. Streptomyces is a well-known antibiotic producer. Marine actinomycetes have emerged as a rich source of novel compounds. Alkaline protease enzyme was isolated from Streptomycetes which has wide applications. In the present study, Streptomycetes were isolated from sediment samples collected from Mandapam coast, Gulf of Mannar and it was characterized. Antimicrobial activity was determined by disc diffusion method for the isolates MS-1 and MS-2 treated against human pathogens such as Salmonella, Klebsiella pneumonia, E.Coli and P. fluorescence. Ms-1 and MS-2 revelaed highest antibacterial alkaline protease activity compared to other isolates. Anti-inflammatory studies using mice model system were performed. The mice were treated with crude extracts of actinomycetes orally inorder to study anti-inflammation. It was found out that wihin 10 days the mice found to be cured from inflammation after oral supplementation of crude enzyme extract which indicated these bioactive compounds have analgesic properties.
Location: TE 15 New Biology Building
Literature cited 1: Amadi, J.E and Adeniyi, D.O. 2009. Mycotoxin production by fungi isolated from stored grains. African of Biotech. 8 (7): 1219-1221. Ahmad, S.K. and Singh, P.L. 1991. Mycofloral changes and aflatoxin contamination in stored chickpea seeds. Food. Addit. Contam. 8: 723-730.
Literature cited 2: Agarwal, V.K. and Singh, O.V. 1974. Routine testing of crop seeds for Fusarium moniliforme with a selective medium. Seed Sci Res.2: 19-22. Barbosa, L.N., Rall, V.L.M., Fernandes, A.A.H., Ushimaru, P.I., Probst, I.S. and Fernandes, A. 2009. Essential oils against foodborne pathogens and spoilage bacteria in minced meat. Food borne Pathog Dis. 6 (6): 725-728.

ID: 59934
Title: Antagonistic effect of essential oils against pathogenic storage Fungi of Cowpea.
Author: A.K. Sarma, M.R. Devi and A. Nigam.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 867-874(2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antifungal, Infective mycoflora, Volatile oil, Vigna unguiculata.
Abstract: The effect of essential oil extracted from the clove (Scyzigum aromaticum), eucalyptus (Eucalyptus oblique), ginger (Zingiber officinale) and peppermint (Mentha piperita) were tested under in vitro condition for their inhibitory effect against twenty numbers of pathogenic fungi isolated from one year stored cowpea (Vigna unguiculata ) seeds at different concentrations (0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09 and 0.1 %) The antifungal activities of the oils were assessed by evaluating the percentage of infected grains. It was recorded that tested oils showed inhibitory effect against the pathogenic storage fungi. The fungi were also responded to the concentration of the oil, i.e. high concentration showed high inhibition. The antifungal activity of the essential oils at 0.02, 0.04, 0.06, 0.08 and 0.1 % concentration was also studied under in vivo condition. Results showed that, application of these oils decreases the incidence of pathogenic storage fungi. It was recorded that, number of fungi appeared at 0.1 % concentration was significantly less. Among the essential oils. The strongest antifungal activity was exhibited by ginger oil.
Location: TE 15 New Biology Building
Literature cited 1: Amadi, J.E. and Adeniyi, D.O. 2009. Mycotoxin production by fungi isolated from stored grains. African J of Biotech. 8 (7): 1219-1221. Ahmad, S.K. and Singh, P.L. 1991. Mycofloral changes and aflatoxin contamination in stored chickpea seeds. Food Addit. Contam. 8: 723-730
Literature cited 2: Agarwal, V.K. and Singh, O.V. 1974. Routine testing of crop seeds for Fusarium moniliforme with a selective medium. Seed. Sci. Res. 2: 19-22. Barbosa, L.N., Rall, V.L.M., Fernandes, A.A.H., Ushimaru, P.I., Probst, I.S. and Fernandes, A.2009. Essential oils against foodborne pathogens and spoilage bacteria in minced meat. Food borne Pathog Dis. 6 (6): 725-728.

ID: 59933
Title: Optimum dose of coagulant for natural surface waters.
Author: A.Khajababau and S.K.Gupta
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 859-866 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Water treatment, Coagulation and flocculation, Jar test, Surface water sources, Turbidity measurements.
Abstract: Conventionally in a water treatment plant fine suspended matter is made to agglomerate into larger size flocs by adding chemicals that neutralize the inherent negative charges on colloidal impurities that repel them from each other and interface with their settling mechanism. It is important that the exact dosage of the chemical (coagulant) is determined prior to addition, because if the dosage becomes more than the particles acquire net positive charge resulting in their repulsion from each other and non-settlement. In water treatment plant, optimum dose of coagulant is generally evaluated by jar test method. Limitation of this test is that it is time consuming, expensive, and the accuracy of results depends upon experience of the plant operator. Coagulant doses are often approximated, resulting in poor quality effluent from secondary settling tanks. In this study water samples were collected locally from different sources e.g. lake, river, village pond a canal. A review of the commonly available coagulants was made and Alum was identified as the potential coagulant for this study. Optimum alum dose tests were carried out on all samples and the results have been analyzed and presented in graphical format to give some idea to plant operator on the approximate values of optimum alum dosages for various sources of water in the region. It is hoped that this study shall reduce the time, effort and costs involved in finding the optimum alum dosages for these surface waters, because the operator can lay his/her hands on rough value as a starting point and then carry out the experimentation to refine his/her results. The study aims to reduce time, cost and effort in operation and maintenance of water treatment plants where turbidity values of the incoming water fluctuate dynamically with time, and offers a sustainable outcome to this issue.
Location: TE 15 New Biology Building
Literature cited 1: Black, A.P. and Chen, C.L. 1965. Electrophoretic studies of coagulation and flocculation of river sediment suspensions with aluminum sulfate. Journal of American Water Works Association. 57 (3): 354-362. John, S.A.A. 1988. Using Moringa seed as coagulants in developing countries.J Am Water Works Assoc.80: 43-50.
Literature cited 2: Lin, S.D. and Evans, R.L.(Personal communication). Physical, chemical and biological changes in water treatment. Illinois State Water Survey, unpublished data. O ' Melia, C.R. and Stumm, W. 1967 .Aggregation of silica dispersions by iron (111) Journal of Colloid and Interface Science. 23 (3): 437-447.