Enter records

ID: 59841
Title: AN INVESTIGATION OF ENZYMATIC KINETIC RESOLUTION OF RACEMIC CLOPIDOGREL.
Author: Yaseer Gaber, Magdy Ali Amin and Rajni Hatti-Kaul.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 247-251 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: None
Abstract: This paper presents a biocatalysis based approach for asymmetric hydrolysis of Clopidogrel, one of the most effective antiplatelet aggregators. Instead of the conventional ?wet lab ?screening method, we used an advanced chemical information retrieval tool, Scifinder ?, to find suitable enzyme candidate (s) for the asymmetric hydrolysis. Scifinder search for reactions similar to the target reaction retrieved three potential hits: horse liver esterase, chemotrypsin and Candida rugosa lipase. Among the three, horse liver esterase was experimentally found to hydrolyse the correct isomer ? of the racemic clopidogrel. Investigation of the effect of different water miscible co-solvents showed DMSO to provide the highest enantiomeric excess (ee) value of 20 % at 43 % conversion of the racemic ester, while a dicyanamide based ionic liquid as a co-solvent showed 12 % ee. This article demonstrates the potential of the chemical databases as possible rapid, resource-efficient tool to find an appropriate selective biocatalyst.
Location: TE 12 New Biology Building
Literature cited 1: Arroyo, M., Torres-Guzman, R., de la Mata, I., Castillon, M.P. and Acebal, C. 2000. Enzyme Microb. Technol., 27: 122-126. Busto, E., Gotor-Fernandez, V. and Gotor, V. 2006. Nat. Protoc., 1: 2061-2067.
Literature cited 2: Chen, C.S., Fujimoto, Y., Girdaukas, G., and Sih, C.J. 1982. J. Am. Chem. Soc., 104: 7294-7299. Chiarello, J.F. Buckwalter, B.L. and Barden, T.C., 2002. Process for the preparation of chiral insecticidal and acaricidal 1, 4-diaryl-2-fluoro-2-butenes via enzymatic hydrolysis of (4-chlorophenyl) cyclopropylethanoic acid methyl ester using esterase. Basf Aktiengesellschaft, Germany p. 75 pp.

ID: 59840
Title: USE OF ANTIBIOTIC AND PROBIOTIC CONTROLLING WATER QUALITY, GROWTH AND SURVIVAL OF SHRIMP LARVAE PENAEUS MONODON FABRICIUS.
Author: Patang.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 241-245 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Shrimp, Antibiotics, Probiotics, Growth and survival.
Abstract: This study is aimed to determine the role of the use of antiobiotics and probiotics in controlling water quality maintenance, growth and survival of shrimp larvae. This study was an experimental study with application of 1 ppm Oxytetracyclin antibiotic and probiotic bacillus at a dose of 0.75 ppm, 1 ppm and 1.25 ppm. Test animals used were shrimp larvae PL-5 which were maintained in 60 L plastic container volume and 9 days after maintenance carried out sampling and day 14 in the total harvest. Biological study of shrimps were tested include growth and survival. Results showed media for maintenance of water quality such as temperature, salinity, pH, oxygen ppm, NH3, nitrite, nitrate, and BOT has a value that can be tolerated for the life of shrimp larvae. Furthermore, growth at the end of the study showed that the highest growth was obtained on treatment B 0.128 g, followed by 0.089 g treatment A, treatment C was 0.074 g and the lowest growth in the D treatment (control ) of 0.010 gr. While the highest survival rate of shrimp test animals highest in treatment C was 84.8%, followed by 78.6 % D treatment, treatment A was 78. 4 % and 77.2 % for treatment B.
Location: TE 12 New Biology Building
Literature cited 1: Ahmad, T. 1988. Farmed shrimp. Bunga Rampai. Coastal Aquaculture Research Centers, Maros. Asni, 2001. Analysis of Bacterial Pathogens in Water, Sediment and Dara clams (Anadara corne) in the waters of the Gulf of Pare-pare. Thesis. Graduate Program Hasanuddin University, Makassar.
Literature cited 2: Atmomarsono, M., Madeali, MI., Tompo, A. and Mulaini, 1993. Infectious bacteria in Pond Water Tiger Shrimp in South Sulawesi. Research Institute for Coastal Aquaculture, Maros. Bakhtiar, 2004. Effectiveness of the use of antibiotics for Disease Control Bacteria Vibrio harveyi in Tiger Shrimp Post larvae of Penaeus monodon Fabricius. Thesis. Graduate Program. Hasaniddin University. Makassar.

ID: 59839
Title: LIPID PRODUCTIVITY IN CHLORELLA PYRNOIDOSA UNDER NITROGEN STARVATION: ENERGY EVALUATION OF AN INTERNALLY ILLUMINATED BUBBLE COLUMN PHOTO BIOREACTOR.
Author: Rajasri Yadavalli, Ramgopal Rao and C.S Rao
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 233-239 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Internally Illuminated Bubble column Photobioreactor, Nitrogen starvation, Lipid productivity, Energy input, Chlorella pyrnoidosa.
Abstract: The effect of nitrogen starvation on the biomass and lipid productivities of Chlorella pyrenoidosa was studied in an internally illuminated bubble column photobioreactor (IIBCPBR) designed on the basis of energy efficiency. Algal culture was grown in the inside column with a culture volume of 4.5 L providing illuminated surface area of 0.1219 m2. The biomass growth rate and total lipid content were 3.12 g/L and 0.482 g/g, respectively, under nitrogen starvation after 8 days of cultivation period. Higher biomass productivity per total energy input (0.57 g W-1 day-1 ) and lipid productivity per total energy input (0.273 g W-1 day-1) were achieved with the IIBCPBR design under nitrogen starvation compared to other alternative designs reported in literature. The proposed IIBCPBR design can potentially reduce the energy input in algal cultivation by as much as 40 % compared to other bubble column photobioreactors.
Location: TE 12 New Biology Building
Literature cited 1: Bligh, E.G. and Dyer, W.J. 1959. A rapid method of total lipid extraction and purification. Can .J. Biochem. Physiol. 37: 911-917. Chiu, S.Y., Kao, C.Y., Chen, C.H., Kuan, T.C., Ong, S.C. and Lin, C.S. 2008. Reduction of CO2 by a high-density culture of Chlorella sp. in a semi continuous photobioreactor. Bioresour Technol. 99: 3389-3396.
Literature cited 2: Hulatt, C.J. and Thomas, D.N. 2011. Productivity, carbon dioxide uptake and net energy return of microalgal bubble column photobioreactors. Bioresour. Technol. 102: 5775-5787. Illman, A.M., Scragg, A.H. and Shales, S.W. 2000. Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb.Technol. 27: 631-635.

ID: 59838
Title: CHARACTERISTICS OF PALM OIL MILL EFFLUENT (POME) IN AN ANAEROBIC BIOGAS DIGESTER.
Author: B. Nurul Adela, N. Muzzammil, S.K. Loh and Y.M.Choo.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 225-231 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Palm oil mill effluent, Anaerobic digestion, Biogas, Microbial population.
Abstract: Palm oil mill effluent (POME) is generated during the palm oil milling process. The wastewater, POME undergoes anaerobic digestion in which biogas is emitted freely or captured as energy. Much research has been conducted to generate biogas from POME but little was focused on the microbial characteristics of the POME which is important to gauge the efficiency in POME treatment. In this study, microbial characterization of POME from an anaerobic digester system operating at mesophilic temperature (34.29 ? 0.8 ? C) was conducted. The system consisted of two 15-m height digester tanks and POME at three different levels-each having a height of 5 m was collected. The gram staining properties of the isolated bacteria at each level were determined via morphological and microscopic studies. It was found that the lowest part of the first tank receiving the incoming effluent exhibited the highest microbial population (1.16 x 106 CFUmL-1), thus facilitating anaerobic digestion of POME for biogas generation. Besides, volatile fatty acid (VFA) decreased from the bottom to the top of the tanks indicating that the system was able to create and maintain a stable and beneficial environment for the active microbial digestion in order to treat POME and to generate biogas efficiently.
Location: TE 12 New Biology Building
Literature cited 1: Appels, L., Baeyerns, J., Degreve, J. and Dewil, R. 2008. Principle and potential of the anaerobic digestion of waste-activated sludge. Progress in Energy and Combustion Science. 34 (6): 755-781. Borja, R. and Banks, C.J. 1995. Comparison of an anaerobic filter and an anaerobic fluidized bed reactor treating palm oil mil effluent. Process Biochemistry. 30 (6): 511-521.
Literature cited 2: Borja, R., Banks, C.J and Salnchez, E., 1996. Anaerobic treatment of palm oil mill effluent in a two stage up-flow anaerobic sludge blanket (USAB) system. Journal of Biotechnology. 45: 125-35. Choorit, W. and Wisarnwan, P. 2007. Effect of the temperature on the anaerobic digestion of palm oil mill effluent. Electronic Journal of Biotechnology. 10: 376-85.

ID: 59837
Title: INTEGRATED WEED MANAGEMENT IN CARROT (DAUCUS CAROTA L)
Author: K.Chaitanaya, S. Amarender Reddy, A.V.N. Lavanya and S. Srinivasa Raju.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 221-223 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Integrated Weed Management, Yield, Carrot.
Abstract: Field experiment was conducted during rabi, 2010-11 at vegetable Research Station, ARI, APHU, Hyderabad to find out effective integrated treatment for wed control in carrot. The experiment consisting of fourteen treatments and three replications was laid out in randomized block design. The results indicated that the pre-emergence application of metribuzin @ 0.3 ha-1 followed by hand weeding at 30 days after sowing recorded the least weed count and dry weight favouring the highest yield of carrot (21.72 t ha-1) An application of pendimethalin c.s. metribuzin and pendimethalin in combination with one hand weeding produced significantly and higher yield of carrot than the application of these herbicides alone.
Location: TE 12 New Biology Building
Literature cited 1: Balraj Singh, 1994. Weed control studies in tomato. Vegetable Science 21: 26-28. Channappagoudar, B.B., Biradar, N.R., Bharmagoudar and Koti, R.V. 2007. Crop weed competition and chemical control of weeds in potato. Karnataka Journal of Agricultural Sciences 20 (4): 715-718.
Literature cited 2: Denesh, G.R., Murthy, K.N.K., Murali, K., Shankaranarayana, V. and Muniyappa, T.V. 2005. Integrated weed management in potato (Solanum tuberosum L). Environment and Ecology 23: Spl-1, 102-104. Govindra Singh, Bhan VM and Tripathi SS 1984 Effect of herbicides alone and in combination with weeding on tomato and associated weeds. Indian Journal of Weed Science XVI: 262-266.

ID: 59836
Title: CHARACTERIZATION AND IDENTIFICATION OF THERMOTOLERANT, FERMENTATIVE YEASTS FROM INDIGENOUS ALCOHOL STARTERS OF FOUR ASIAN COUNTRIES.
Author: Win Aung, Yuka Watanabe and Fumio Hashinaga.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 211-220 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Indigenous alcohol starters, Thermotolerant fermentative Yeasts, Industrial use, Conventional and molecular techniques.
Abstract: Of 73 yeast strains isolated from indigenous alcohol starters of Nepal, Myanmar, Cambodia and Indonesia, 7 strains that grew and fermented at above 42 ? C using the selected yeast strains, and the ethanol productivities were measured after 48 h incubation. Two strains, MN 1-3 and CS2-3 were found to be higher ethanol productivity of 6.2 and 6.7 % (w/v), respectively. Thermotolerant, fermentative yeast strains were identified by conventional and molecular techniques. In the molecular technique, polymerase chain reaction (PCR) amplified 18s r DNA and internal transcribed spacer 1 (ITS 1) genes using specific primers were analyzed. As a result, the strains selected were identified as follows: NP3-2 and MN1-5, Issatchenkia orientalis; MN1-3 and CS2-3, Saccharomyces cerevisiae; CS1-1, Pichia fabianii; RA2-1, Clavispora lusitaniae and RA3-3, Candida glabrata.
Location: TE 12 New Biology Building
Literature cited 1: Altschul, S.F., Madden, T.L., Schaffer A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research. 25: 3389-3402. Aung, W., Watanabe, Y. and Hashinaga, F. 2012. Isolation and phylogenetic analysis of two thermotolerant, fermentative yeast strains from liquid tape ketan (Indonesian rice wine). Food Science and Technology Research. 18: 143-148.
Literature cited 2: Barnett, J.A., Payne, R.W. and Yarrow, D. 2000. Yeasts: Characteristics and Identification. Cambridge University Press. Chaijamrus, S. and Mouthung, B. 2011. Selection of Thai starter components for ethanol production utilizing malted rice from waste paddy. Songklanakarin Journal of Science and Technology. 33: 163-170.

ID: 59835
Title: GGDM MODEL ON VALIDATION OF THE INTERIOR BUIDING LAYOUT DESIGN PARAMETERS TOWARDS ENERGY EFFICIENCY AND USER COMFORT.
Author: Mohammad Pir Mohammadi, Abdullah Sani Ahmad, Gurupiah Mursib, Mohsen Roshan and Mahdi Torabi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 205-210 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Energy Efficiency, User Comfort, Ground Group Decision Making Model.
Abstract: Collective decision making model emerges to be applied in the cases where decision makers are not capable to seat in a meeting. Grounded Group Decision Making (GGDM) is unique since collective decision making aggregated by cross evaluation of decision makers in GGDM process. The GGDM in this study is applied to validate the uncertainty involved in validation of the building interior layout design parameter towards energy efficiency and comfort strategies. This problem is applied as illustration of applicability of GGDM in establishing a benchmark for evaluation of students in energy efficient architectural design. First, a literature review and relevant definition to each interior layout design parameter is presented. Second, a GGDM validation study conducted amongst twenty one lecturers and professional practitioners (industrial collaborators of university) in Faculty of Built Environment, Universiti Teknologi Malaysia. In result of the study seven parameters are validated and rated respectively. In implication of specific result of this study, these are useful for researchers in enhancement of Automated Computer Aided Building towards auto evaluation of students drawing in consideration of comfort and energy efficiency.
Location: TE 12 New Biology Building
Literature cited 1: Arnheim, R. 1974. Art and Visual Perception. University of California Press. Cagan, J., Shimada, K. and Yin, S. 2002. A survey on computational approach of three-dimensional layout problems. Computer-Applied Design, 34: 597-611.
Literature cited 2: Deasy, C.M., and Lasswell, T.E 1985. Designing Places for People. Watson-Guptill. Evans, Gary W., Lepore, Stephen J., Schroeder, and Alex. 1996. The role of interior design elements in human responses to crowding. Journal of Personality and Social Psychology. 70 (1): 41-46.

ID: 59834
Title: EFFECTS OF SECONDARY METABOLITES STATUS OF BACILUS SPP. ON FUNGAL PATHOGEN ALTERNARIA ALTERNATA
Author: T.Angayarkanni and Anitha Subash.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 201-204 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Alternartia alternate, Bacillus subtilis, Salicylic acid, Siderophore and Antiobiotics like surfactin and iturin.
Abstract: Alternaria diseases are among the most common diseases of many plants throughout the world. They affect primarily the leaves, stems, flowers and fruits of annual plants, especially vegetables and ornamentals. In this study the in vitro screening of antagonistic Bacillus spp. against Alternaria alternata revealed that the maximum inhibitory effect on the mycelia growth was exhibited by the isolates AUB4 and AUB3.Maximum siderophore production was observed in the isolate AUB7.Estimation of salicylic acid production by the Bacillus spp. isolates showed that isolate AUB4 produced maximum amount. Crude extract of Bacillus antibiotics showed that isolates AUB6, AUB4, AUB2 AND AUB1 have maximum inhibitory effect on the mycelial growth of Alternaria alternate. The TLC analysis of antibiotics extracted from isolates AUB7 and AUB4 showed Rf values of 0.63 and 0.69 for surfactin and 0.48 and 0.53 for iturin A respectively. Thus, from the present study, it can be concluded that among the several secondary metabolites produced by the Bacillus spp isolates, siderophores, salicylic acid and antibiotics are significantly involved in the suppression of the growth of Alternaria alternate. Among the ten isolates screened, AUB4 and AUB7 effectively controlled the growth of the fungal pathogens. This may be due to the high production of siderophore, salicylic acid and antibiotics like surfactin and iturin.
Location: TE 12 New Biology Building
Literature cited 1: Araujo, F.F, Henning, A.A. and Hungaria, M. 2005. Phytohormones and antibiotics produced by Bacillus Subtillis and their effects on seed pathogenic fungi and on soybean root development. World Journal of Microbiology and Biotechnology. 21: 1639-1645. Cho, S.J., Lim, W.J., Hong, S.Y., Park, S.R. and Yun, H.D. 2003. Endophytic colonization of ballon flower by Antifungal strain Bacillus sp. CY22.J. Biosci.Biotechnology.Biochem. 67: 2132-2138.
Literature cited 2: Deshwal, V.K., Pandey, P., Kang, S.C.and Maheswari, 2003. Rhizobacteria as a biological control agent against soil form plant pathogenic fungi. J. Indian Journal of Experimental Biology. 41: 41: 1160-1164. Gardener, M.B.B. 2004. Ecology of Bacilus and Paenibacillus spp. in agricultural systems. J. Phytopathol. 94: 1252-1258.

ID: 59833
Title: OPTIMAL PRODUCTION OF EXOPOLYSACCHARIDE BY PAENIBACILLUS HUNANENSIS STRAIN Y-22 ISOLATED FROM SOIL.
Author: Grenil Menezes and K. Aruna.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 189-199 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Paenibacillus hunanensis strain Y22, Exopolysaccharide
Abstract: An exopolysaccharide (EPS) producer was isolated from soil sample by primary screening. It was identified as Paenibacillus hunanensis strain Y22 on basis of biochemical studies and 16S rDNA gene sequence analysis. Semi-defined medium composition and other physical parameters viz, pH, temperature and time were varied for maximum exopolysaccharide production. The effect of variable carbon and nitrogen sources were also investigated.Paenibacillus hunanensis strain Y22 in semi-defined medium (SDM) Galactose (60 g/ L), Tween 80 (1ml/L), Yeast Nitrogen base (5g/L), Bacto-casitone (10 g/L), MgSO4 (0.1 g/L), NaNO3 (2g/L) pH-6.5 under shaker conditions (120rpm) for 6 days at 30? C produced 32 g/L of EPS. The other growth parameter like absence of Na-acetate, K2HPO4 and MnSO4 in SDM also showed remarkable increase in EPS yield. HPTLC analysis of EPS from Paenibacillus hunanensis strain Y22 showed presence of galactose, glucose, fructose and mannose. EPS produced by this organism also exhibited adhesive and emulsifying property, whereas Paenibacillus hunanensis strain Y22 exhibited antibacterial activity against some gram positive bacteria.
Location: TE 12 New Biology Building
Literature cited 1: Aguilera, Margarita, Monteoliva-Salnchez, Mercedes, Squrez Antonio, Guerra Victor, Lizama Catherine, Bennasar Antonio and Ramos-Cormenzana Alberto, 2001. Paenibacillus jamilae sp. nov., an exopolysaccharide-producing bacterium able to grow in olive-mill wastewater. International Journal of Systematic and Evolutionary Microbiology. 51: 1687-1692. Ash, C. Priest, F.G. and Collins, M.D. 1993. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus.Antonie Leeuwenhoek. 64: 253-260.
Literature cited 2: Ashtaputre, A.A. and Shah, A.K. 1995. Emulsifying property of a viscous exopolysaccharide from Sphingomonas paucimobilis. World Journal of Microbiology & Biotechnology. 11: 212-219. Azeredo, J. and Rosario, O. 1996. A New Method for Precipitating Bacterial Exopolysaccharides. Biotechnolgy Techniques. 5: 341-344.

ID: 59832
Title: ANTIOXIDANT ACTIVITY OF ACALYPHA ALNIFOLIA KLEIN EX WILLD LEAF EXTRACT.
Author: Vasthi Kennedy Evanjelene and Devarajan Natarajan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 185-188 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Acalypha alnifolia, Antioxidant.
Abstract: Antioxidants are vital substances which possess the ability to protect the body from damages caused by free-radical induced oxidative stress. A variety of antioxidants is found in dietary sources like fruits, vegetables and tea. The purpose of present study was to examine and determine in vitro antioxidant potential of methanol and aqueous extracts of Acalypha alnifolia, and to determine the IC50 values. The nitric oxide, super oxide and hydrogen peroxide radical scavenging assay were also analyzed. The best IC50 value was noticed in methanol extract of all tests i.e, scavenging assay were also analyzed. The best IC50 value was noticed in methanol extract of all tests i.e., scavenging activity of nitric oxide (> 1000mg/mL), hydrogen peroxide (550 mg/mL), super oxide (44.33 mg/mL) respectively. The overall results highlight that methanolic extract possess higher antioxidant activity than aqueous extract.
Location: TE 12 New Biology Building
Literature cited 1: Anandakumar, A.M., Paulsamy,S., Sathish Kumar, P. 2009. Preliminary phytochemical studies for the quantification of secondary metabolites of medical importance in the plant Acalypha fruiticosa Forssk. J. Appl Nat. Sci., 1 (1): 41-43. Anderson et al., 2001. The prevalence of Comorbid Depression in Adults With Diabetes,doi: 10.2337/diacare. 24.6.2001. Diabetes Care 24 (6): 1069-1078.
Literature cited 2: Balakrishanan, V., Prema, P., Ravindaran, K.C. and Philip Robinson,J. 2009. Ethnobotanical Studies among Villagers from Dharapuram Taluk. Global Journal of Pharmacology.3 (10): 08-14. Buyukokuroglu, M.E., Oktay, M. and Kufrevioglu, O.I. 2001. In vitro antioxidant properties of dantrolene sodium pharmacol. Res.44: 491-95.

ID: 59831
Title: PERFORMANCE OF IMMOBILIZED BACILLUS SUBTILIS SUBSP. SUBTILIS "RA-29" IN CONGO RED DECOLOURIZATION
Author: Rajesh Sawhney, Arun Kumar, Robina and Jayata
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 179-184 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Bacillus subtilis, Congo red, Immobilization, Calcium alginate, Reusability.
Abstract: Congo red, a carcinogenic diazo dye is discharged through industrial effluents in the environment. The removal of this hazardous dye from effluents is important for health and environment protection. In recent past, immobilization has come up as a promising biological tool. The present study highlights the immobilization of Bacillus subtilis sub sp. subtilis RA-29? on a suitable matrix and the potential of immobilized whole cells to decolourize congo red. The whole cell immobilized calcium alginate beads were stable for 7 days. The immobilized RA-29 exhibited cell density dependent decolourization. Maximum congo red decolourization (81.55%) was achieved at cell density of 30 ug/mm3 after 48 hr of incubation at 37? C under static condition. The calcium alginate immobilized ?RA-29? showed operational stability with efficient decolourization up to five cycles with minimum decolouriztion percentage falling to 61.11 %.The immobilized Bacillus subtilis could be commercially viable biotechnological tool for bioremediation of dye contaminated industrial effluents.
Location: TE 12 New Biology Building
Literature cited 1: Bryant, S.J, Nuttleman, C.R. and Anseth, K.S. 2000. Cytocompatibility of UV and visible light photoiniating systems on cultured NIH/ 3T3 Fibroblasts in vitro. Journal of Biomaterial Science Polymer Edition. 11: 439-457. Chang, J.S, Chou, C. and Chen, Y.S. 2001. Decolourization of azo dyes with immobilized Pseudomonas leteola. Process Biochemistry. 36 (8-9): 757-763.
Literature cited 2: Chen, K.C. Wu, J.I, Huang, C.C, Liang, Y.M. and Hwang, SzCJ. 2003. Decolourization of azo dye using PVA-immobilized microorganisms. Journal of Biotechnology. 101 (3): 241-252. Couto, S.R. 2009. Dye removal by immobilized fungi. Biotechnology Advances. 27: 227-235.

ID: 59830
Title: ANTIBIOTIC RESISTANCE PATTERN OF SOME CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS IN THE HOSPITALS OF AKOLA, MAHARASHTRA, INDIA.
Author: A.M.Banole and V.D. Nanoty.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 175-177 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antibiotic resistance, Staphylococcus aureus, Hospital, Vancomycin resistance.
Abstract: Antibiotic resistance pattern of 187 clinical isolates of staphylococcus aureus was studied. Maximum resistance was observed against viz., Ampicillin, Azolocillin, Clindamycin, Ciprofloxacin, Co-trimoxazole, Ofloxacin, Vancomycin, Nitrofurantoin and Ticarcillin also showed high percentage of resistance to it. All the isolates from Government hospital showed higher resistance to all antibiotics than that of charitable and private hospital.Vancomycin resistance was found between 80 to 95 % for isolates from government, private and charitable hospital.
Location: TE 12 New Biology Building
Literature cited 1: Amarnath Satish Kumar, 2003. The silent killer in hospital. Highlight the hidden perils of hospitals acquired infections. www.vasanthvisuals.com/hospital.html (The New Indian Express, Bangalore, April 7, 2003.) Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turk, M. 1996. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol. 45: 493-496.
Literature cited 2: CDC, Reduced susceptibility of Staphylococcus aureus to Vancomycin, 1997. MMWR. 46: 624-626. Chakravarty, A., Talwar, V. and Gupta, H. 1988. Antibiotic resistance pattern of Staphylococcus aureus with special reference to methicillin resistance strains. Indian J. Med. Res.87: 570-572.

ID: 59829
Title: SANITARY ANALYSIS OF SITARAAM JAJU SAGAR DAM, NEEMUCH, MADHYA PRADESH.
Author: Sonu Chouhan, Archana Pancholi and Ritu Vyas.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 167-173 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Coliforms, contamination, drinking water, pathogens, sanitation, total viable count.
Abstract: Diseases from dirty water and lack of sanitation kill more people every year than all forms of violence including war. Priority needs to be given to improving and developing the drinking water supplies that represent the greatest public health risk. Improving access to safe drinking water can result in tangible benefits to health. Every effort should be made to achieve a drinking water quality as safe as practicable. AIM: The present research was intended to obtain information about the sanitary situation of Sitaram Jaju Sagar Dam. The dam is the sole source of drinking water to the inhabitants of Neemuch. The Dam water is treated by Hingoria Water Treatment Plant through Flocculation by Alum, Rapid Sand Filtration and final chlorination by chlorine. This treated water is directly supplied to the community without any bacteriological testing; hence this study aims to evaluate the microbiological quality of water and comparison of the results with the National and International Standards for drinking water. METHODOLOGY: Water samples from the Dam, outlet of plant and Household tap were collected twice a month and analyzed for Total Viable Count, Total Coli forms and presence of water borne Pathogens, using Standard methods (APHA, 99). RESULTS & DISCUSSION: The results revealed that water samples from the dam, Hingoria plant and household tap failed to meet the standards, throughout the year and therefore are not acceptable for drinking. The bacterial isolates recovered from the samples are-V.cholerae, Salmonella, Shigella, P. aeruginosa, S. aureus, and E.coli. CONCLUSION: From the results, it can be concluded that the dam water is highly contaminated with fecal matter, domestic sewage, soil and agricultural runoff, effluents from septic system and is not suitable for drinking; moreover the Hingoria Treatment Plant is inefficient to treat such a highly contaminated water to make it suitable for drinking. RECOMMENDATIONS: Hingoria Treatment Plant must be upgraded and supplied water must be regularly assessed for the presence of coli forms at its source and consumer point areas before an outbreak of epidemic occurs.
Location: TE 12 New Biology Building
Literature cited 1: Aneja K.R. 2008. Experiments in Microbiology plant Pathology and Biotechnology. New Age International (P) Limited Publishers Ed 4: 361-368. A.P.H.A. 1999. Standard Methods for the examination of water and waste water. 21th ed. American Public Health Association, 1015. Fifteenth street, NW, Washington, DC.
Literature cited 2: A.P.H.A. 1995. Standard Methods for Examination of Water, Sewage and Industrial Wastes: 19th ed. American Public Health Association, Washington, U.S.A. APHA1998. Standard methods for the examination of water and wastewater. 20th ed. American Public Health Association, Washington, DC.

ID: 59828
Title: EVALUATION OF DIFFERENT MEDIA FOR FERMENTATIVE PRODUCTION OF BIOPIGMENTS USING YEAST CULTURES.
Author: Reeba Panesar, Paramjit S. Panesar and Manab B.Bera.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 161-166 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Yeast, Rhodotorula, Kinnow peel, Media, Biopigments.
Abstract: In the present investigation, six different media were evaluated for the production of bio pigments using two yeast strains. The synthetic media based on glucose was compared with kinnow peel based media supplemented with different ingredients. The various combinations of chemical constituents as well as salts were added to the media to increase the biopigment production and minimize the cost of production process. The comparative studies indicated that Rhodotorula sp. MTCC 1446 has better potential for pigment production from kinnow peel based media. Further, taro leaves can be good supplement for pigment production to improve the economics of the process. The comparison of extraction methods indicated that acetone-ether extraction method was found to be better method than acid extraction method.
Location: TE 12 New Biology Building
Literature cited 1: Ahmed, M.M., Nomani, M.S and Panda, B.P. 2009. Screening of nutrient parameters for red pigment production by Monascus purpureus MTCC 369 under submerged fermentation using placket-burmen design. Chiang Mai. J. Sci. 36: 104-109. Aksu, Z. and Eren, A.T. 2005. Carotenoid production by the yeast Rhodotorulla mucilaginosa: Use of agricultural wastes as a carbon source. Process Biochem. 40: 2985-2991.
Literature cited 2: Aksu, Z. and Eren, A.T. 2007. Production of carotenoids by isolated yeast of Rhodotorula glutinis. Biochemical Engg. J 35: 107-113. An, G.H., Jang, B.G. and Cho, M.H. 2001. Cultivation of the carotenoid-hyperproducing mutant 2A3N of the red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) with molasses. J. Biosci.Bioeng. 92: 121-125.

ID: 59827
Title: CANDIDAL BIOFILM: AN OUTLOOK ON THE MORPHOLOGICAL ASPECT AND FORMATION.
Author: Florida Primrose and Nithya Jagannathan.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (1) 155-159 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Adherence, Biofilm, Candida, Matrix, Invasion
Abstract: Candida is the dimorphic fungi which is the most common microbes present in the body. Biofilms are communities of microorganisms which are often attached onto a surface and enclosed in an extracellular polysaccharide matrix. The formation of a mature biofilm is based on the transition between the hyphae and the yeast forms of the candidal species, cell adhesion and the extracellular matrix formation. Biofilm formation is a major virulence factor in the pathogenecity of Candida, and Candida biofilms are difficult to eradicate because of their very high antifungal resistance. Candida can cause both superficial and systematic disease, and are now recognized as major agents of hospital-acquired infection. Numerous studies have been performed to determine the role of biofilm in infections. The current review focuses on the steps involved in biofilm formation, molecular characteristics and the factors which play a role in biofilm formation with a mention on the drug resistance.
Location: TE 12 New Biology Building
Literature cited 1: Alem, M.A. and Douglas, L.J. 2005.Prostaglandin production during growth of Candida albicans biofilms. J. Med Microbiol. 54: 1001-1005. Al Fattani, M.A and Douglas, L.J. 2006. Biofilm matrix of Candida albicans and Candida tropaicalis: chemical composition and role in drug resistance. J. Med Microbiol.55: 999-1008.
Literature cited 2: Andes, D., Nett, J., Oschel, P., Albrecht, R., Marchillo, K. and Pitula, A. 2004. Development and characterization of an in vivo central venous catheter Candida albicans biofilm model. Infect Immun. 72: 6023-6031. Bachmann, S.P., VandeWalle, K., Ramage, G., Patterson, T.F., Wickes, B.L., Graybill, J.R and Lopes-Ribot, J.L. 2002. In vitro of caspofungin against Candida albicans biofilms. Antimicrob Agents Chemother. 46 (11): 3591-3596.