ID: 59856
Title: A STUDY OF CYANOBACTERIAL METABOLITE PRODUCTION DURING DIFFERENT INCUBATION PERIODS USING OSCILLATORIA AND SYNECHOCYSTIS SPP
Author: P.K.Chouhan, P.Das and M.Sharma.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 339-342 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Cyanobacteria, Protein, Amino acids and incubation period.
Abstract: Protein and amino acids are nitrogenous compounds and are also used as precursor molecule in protein synthesis. A lot of cellular and membrane components are made up of protein; therefore organisms synthesized amino acids and protein during growing condition as primary metabolites. A cynobacterium is most diverse photosynthetic nitrogen fixing group and their thallus vary from unicellular to filamentous. They found in terrestrial habitat and also in water and fix atmospheric nitrogen in both micro-aerophilic and aerobic condition, in aerobic condition nitrogen fix by a specialized cell found only in filamentous forms called heterocyst. This fixed nitrogen assimilated in form of amino acids and then proteins. Protein, amino acids, vitamins and plant growth hormones are liberate by cyanobacteria in culture media; therefore these nitrogenous compounds improve overall soil fertility. Our study based on estimation of total protein and identification of amino acids in culture filtrate, cyanobacterial strains were isolated from Botanical garden soil of Govt. PG College, Mandsaur. This isolates were subjected to extracellular metabolite productions using BG-11 liquid medium with nitrogen, thereafter metabolites were estimated/ identified using standard protocols.
Location: TE 12 New Biology Building
Literature cited 1: Adam, M.S. 1999. The promotive effect of the cyanobacterium Nostoc muscorum on the growth of some crop plants. Microbiological Polinica. 48: 163-171.
Allen, M.M. 1968. Simple conditions for growth of unicellular blue-green algae on plates. J. Phycol. 4: 1-13.
Literature cited 2: Allen, M.M. 1973. Methods for cynophyceal.Stein, J.R. (ed). Handbook of phycological methods, culture methods and growth measurements, Cambridge Univ. press pp. 127-138.
Aref-Elham, M.M. 2001. Studies on Cynobacteria in Egypt. Ph. D. Thesis, Fac. Agric. Cairo Univ. Egypt.
ID: 59855
Title: SOLID WASTE MANAGEMENT USING VERMICOMPOSTING AND KODMICTM BIO-PEDESTAL COLUMN AND ITS UTILITY AS ORGANIC MANURE.
Author: M.G.Sumi, M. Vani, Dona V.Idicula and K.D. Mini.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 333-338 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Solid Waste, Biodegradable, Vermicomposting, Kodmic Bio-Pedestal Column, Organic Manure.
Abstract: Solid waste management is one of the biggest environmental problems faced by us today. Much of the residual waste materials of anthropogenic and animal origin, if not properly disposed, end up polluting terrestrial and aquatic systems. A large proportion of solid waste can be categorized as organic and biodegradable. Such degradable organic waste can be converted to useul and or/ environmental benign products using techniques such as microbial composting, vermicomposting, biogas plant, etc; there by. In the present study, we have used vermicomposting and KODMiC bio-pedestal column (KBPC; invented by Dr. Ignatious Konnikkara, Professor, Dept of Life Sciences, Calicut University, Kerala) two solid waste management methods to degrade house-hold waste. Solid waste was converted to organic manure using vermicomposting and KBPC method. Plant growth response, soil manured with vermicompost showed faster germination, flowering and fruiting time, while plants grown around KBPC unit were normal, healthier and had higher yield than the control plants. Increase in the microflora, i.e. the total number of bacterial and fungal colonies was observed in both methods, from the 0th-45th day. There was a increase in the NPK concentration compared to the control. Both methods had a major role in converting biodegradable waste into organic manure and prompted many plant growth characteristics. As a solid waste management option for a day to day biodegradable household wastes, KPBC method had several advantages over composting, as being a simple, cost effective, low maintenance, easy installation and did not need any expertise.
Location: TE 12 New Biology Building
Literature cited 1: Aneja K.R. 1996. Experiments in Microbiology, Plant Pathology and Biotechnology. K.R. Aneja, New Age International (P) Limited, New Delhi. 2nd ed.
Arancon, N.Q., Edwards, C.A., Atiyeh, R.M. and Metzger J.D. 2004. Effects of vermicomposts produced from food waste on green house peppers. Bioresource Technology.
Literature cited 2: Atiyeh, R.M., Edwards, C.A., Subler, S. and Metzger, J.D. 2000b. Earthworm-processed organic wastes as components of horticulture potting media for growing marigold vegetable seedlings. Compost Science and Utilization. 8 (3): 215-223.
Atiyeh, R.M., Lee, S.S., Edwards, C.A., Arancon, N.Q. and derived from earthworm-processed organic wastes on plant growth. Bioresource Technology. 84: 7-14.
ID: 59854
Title: GC-MS ANALYSIS OF PIMPINELLA TIRUPATIENSIS, BAL & SUBR. TUBER EXTRACT: AN ENDEMIC PLANT TO TIRUMALA HILLS OF EASTERN GHATS, INDIA.
Author: P.Uma Maheshwari, S.K.M. Basha, M.Rambabu and C.V.Narasimha Murthy.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 329-332 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: GC-MC Analysis, Pimpinella tirupatiensis-tuber chemical composition
Abstract: Pimpinella tirupatiensis Bal & Subr. (Family Apiaceae; local name, kondakothimera) is a rare and endemic medicinal plant and restricted to the Seshachalam hills of the Eastern Ghats, India (Balakrishnan and Subramanyam 1960, Nair and Sastry 1998). The whole plant of P .tirupatiensis is used to treat cough, stomach, liver problems, asthma, ulcer and tooth ache (Reddy and Reddy, 1998, Madhava Chetty et al., 2008). This plant root extract is also used to treat skin disease and also as antimicrobial agent (Jeevan Ram et al. 2004, Bakshu and Venkata Raju 2004). The aim of the study is to investigate the phytochemical analysis of alcoholic tuber extract of Pimpinella tirupatiensis Bal. & Subr by qualitative and GC-MS methods. The qualitative studies of the tuber revealed the presence of Tannins, Steroids, Alkaloids, Glycosides, Flavonoids and Phenolic compounds. In the GC-MS analysis, 24 bioactive phytochemical compounds are identified in the alcoholic extract. Among them two important photochemical compounds Germacrene D (4.35 %) & and a-Humulene (1.45.6 %) are present in rich amount. Germacrene are a class of volatile organic hydrocarbons, specifically, sesquiterpenesy produced in certain plant species and they are known for their antimicrobial and insecticidal properties, though they also play a role as insect pheromones. P-Cymen is a volatile oil which found to have antioxidant property.
Location: TE 12 New Biology Building
Literature cited 1: Adams, R. 1995. Identification of essential oil components by Gas Chromatography/Mass Spectroscopy. Allured Publishing Co., Carol Strea, IL. Delazar, A., E. Nazifi, A. Movefeghi, L. Nahar, H. Nazemiyeh, S.B. Moghadem, S. Asnaashari and Sarkar, S.D. 2009. GC-MS analysis of ornithogalum Procerum. Daru. 17: 33-36.
Bakshu, L.M.D., Venkata Raju, R.R. 2002. Essential oil composition and antimicrobial activity of tuberous roots of Pimpinella tirupatiensis Bal. and Subr., an endemic taxon from eastern ghats, India. Flavour and Fragrance Journal. 17: 413-415.
Literature cited 2: Balakrishnan, N.P. and Subramanyam, K. 1960. Bull. Bot. Surv. India. 427-428.
Harborne, J.B. 1973. Phytochemical Methods: Guide to Modern Techniques of Plant Analysis. Chapman and Hall, London.
ID: 59853
Title: EXPLORATION OF INDUSTRIALLY IMPORTANT ENZYME PRODUCING MICROORGANISMS FROM EXTRTEME FROM HABITATS OF DIBANG VALLEY, ARUNACHAL PRADESH, INDIA.
Author: Kodaparthi Ashwitha, G.Balakrishna, Pavan Kumar Pindi and A.Swaroopa Rani.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 321-327 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Extreme habitats, extremophiles, Cellulases, Lipases, Proteases and CFU.
Abstract: Microbial diversity constitutes the most extraordinary reservoir of life in the biosphere that we have only just begun to explore and understand. A preliminary investigation on the microbial diversity of potential extracellular enzyme producers was done by direct screening methods to provide an insight on the possibility to use different moderate extremophiles as a source of extremophilic enzymes in biotechnological processes. Samples were collected randomly from soil sediments of Dibang valley, Arunachal Pradesh, India and their physico-chemical characteristics such as pH, moisture content and Nitrogen, Phosphate and Potassium content were determined. The dynamics of microbial population was investigated by using serial dilution method on different microbiological media to ensure maximum microbial diversity. The isolated bacteria, actinomycetes and fungi were screened for their cellulose, protease and lipase activities. The study revealed that bacterial isolates showing higher zone of cellulolytic activity belong to Bacillus, Pseudomonas and Serrartia sp, while fungal isolates mostly belong to Aspergillus and Penicillium sp. The study has suggested that the potential enzyme producing isolates were identified indicating that the extreme habitat soils are rich source of industrially important enzyme producers.
Location: TE 12 New Biology Building
Literature cited 1: Adinarayana, K.P., Ellaih, D.S. Prasad. 2003. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS. Pharm. Sci. Tech. 56 (4): 1-9.
Ajwa, H.A., Dell, C.J. and Rice, C.W. 1999. Changes in enzyme activities and microbial biomass of tall grass prairie soil as related to burning and nitrogen fertilization. Soil Biol. Biochem. 31: 769-777.
Literature cited 2: AOAC 1990. Official Methods of Analysis. 15th ED. AOAC, U.S.A.
Arpigny, L.L and Jaeger, K.E. 1999. Bacterial lipolytic enzymes: classification and properties. Biochemistry Journal 343: 177-183.
ID: 59852
Title: DECOLOURIZATION OF TEXTILE AZO DYES BY LACCASE PRODUCING BACILLUS SPP.ISOLATED FROM SOIL.
Author: Viraj Krishna Mishra and Himani Sharma.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 313-319 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Azo dyes, Spore Laccase, Bacillus Spp, Guiacol, Dye decolorization.
Abstract: Twenty five bacterial isolates exhibiting laccase screened on the basis of 0.1 % guaiacol utilization from field soil. LB media containing Cu2+ was used for isolation screening. Out of twenty five only three isolates were selected on basis of dye decolorization activity and identified as Bacillus spp. on basis of biochemical characterization. Selected isolates D1023, D1032 and D1033 were transferred to 2xSG medium to increase and fasten spore production. Selected isolates could grow from temperatures ranging from 30 ? C to 55 ? C and showed optimum growth temperature and pH at 37 ? C and 7.0, respectively. Maximum growth was observed using fructose as carbon source and at 2.6 % concentration of nutrient broth. Spore laccase of isolates D1023, D1032, D1033 exhibited the excellent potential to decolorize different azo dyes: Amido Black, Orange G, Methyl Orange, Congo Red and Solochrome Black at a concentration of 100 mg/L maximum up to 83. 43 %, 91.42 %, 78. 14 %, 72.42 % and 71.31 % respectively during a time period of 4 days at 37 ? C (pH 7) during static conditions, which suggests the potential application of spore laccase in dyestuff treatment.
Location: TE 12 New Biology Building
Literature cited 1: Asad, S., Amoozegar, M.A., Pourbabaee, A. A., Sarbolouki, M.N. and Dastgheib, S.M.M. 2007.Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresource Technogyl 98: 2082-2088.
Bras, R., Gomes, A., Ferra, M.I.A., Pinheiro, H.M, Gonc and alves I.C. 2005. Monoazo and diazo dye decolorisation studies in a methanogenic UASB reactor. J. Biotechnol. 115: 57-66
Literature cited 2: Banat, I.M., Nigam, P., Singh, D. and Marchant, R. 1996. Microbial decolorization of textile-dye containing effluents: a review. Bioresource Technology. 58: 217-227.
Chung, K.T. and Cerniglia, C.E. 1992. Mutagenicity of azo dyes: structure activity relationship. Mutation Research. 277: 201-220.
ID: 59851
Title: PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL ANALYSIS OF SOLANUM MURICATAM FRUIT EXTRACTS.
Author: A. Nirmala, G.Manimekalai, P. Vasanthi, A. Jagajothi and V.K Evanjelene.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 307-311 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Solanum muricatam, Antimicrobial, Phytochemicals, Medicinal plant.
Abstract: Medicinal plants are a valuable natural resource and regarded as potentially safe drugs. The main objective of the present research work was to see antidiabetic activity of Solanum muricatam fruit extracts obtained by different (Hexane, Ethyl alcohol and Hydro alcohol) extracts. Phytochemical screening showed positive results for alkaloids, flavonoids, steroids, terpenoids, phenols, tannin, carbohydrates, oil and resins in fruit extracts. The extracts showed rich phytochemical activity. Antimicrobial activity was evaluated against different bacterial strains (both gram positive and gram negative). It was observed that hydro alcohol extract of Solanum muricatam shows better activity in S. typhi ' A ' (40 mg/well) was the most potent extract which showed significant inhibition of the growth of bacterial strains.
Location: TE 12 New Biology Building
Literature cited 1: Bauer R.W., Kirby M.D.K., Sherris J.C. and Turck M. 1966. Antidiabetic susceptibility testing by standard sigle disc diffusion method. American Journal of Clinical Pathology.45.
Bauer, R.W. and Kirby, M.D.K., Sheris, J.C. Turck, M. 1966. Antibiotic susceptibility testing by standard single disc diffusion method. American Journal of Clinical Pathology. 45: 493-496.
Literature cited 2: Chesson, A., C.S. Stewart and R.J. Wallace, 1982. Influence of plant phenolic acids on growth and cellulytic activity of rumen bacteria. Applied envir.Microbiol., 44: 597-603.
Chioma, A., Anosike, Onyechi Obidoa and Lawrence, U.S. Ezeanyika 2012. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum) DARU Journal of Pharmaceutical Sciences. 20: 76.
ID: 59850
Title: ISOLATION AND CHARACTERIZATION OF RHIZOBIAL ISOLATES FROM THE RTHIZOSPHERIC SOIL OF AN ENDANGERED PLANT MEIZOTROPIS PELLITA.
Author: Pankaj, Tapan Kumar Nailwal, Lalit Singh and Amit Panwar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 301-306 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Meizotropis pellita, PGPR, Rhizosphere, nif genes.
Abstract: Meizotropis pellita (vern, Patwa) an endangered plant species found on the three sites in Patwadang 1r Nainital Uttarakhand. Meizotropis pellita is an angiospermic plant which belongs to family fabaceae. Most of the fabaceae family plants fix atmospheric nitrogen. Patwa belong to this family, so rhizobacterial spp. from its rhizospheric soil were isolated and characterized. This study focuses on the important role of Rhizobacteria associated with the Rhizospheric soil of Meizotropis pellita. By the biochemical properties of the Rhizobial isolates it is proved that these Rhizobial isolates have potent plant growth promoting abilities (PGPR). PGPR properties of Rhizobial isolates is based on their phosphate solubilisation property. All strain showed phosphate solubilisation property. Seven different rhizobacterial strains of bacteria were isolated from the rhizosphere of Patwa plant. Biochemical characterization was done for their identification, and molecular characterization authenticated that the isolated bacteria can fix atmospheric nitrogen and this may have PGPR activity. Nif genes expresses and produce nitrogenase enzyme which is mainly responsible for nitrogen fixation. In this study we used PCR amplifications of nif genes present in the isolated bacterial strains. All seven isolate showed nif gene amplification. This is the first study associated with the Rhizospheric region of the M. pellita plant.
Location: TE 12 New Biology Building
Literature cited 1: Ahmad, F., Ahmad, I. and Khan, M.S. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res. 163: 173-81.
Bazzicalupo, M. and Fani, R. 1995. The use of RAPD for generating specific DNA probes for microorganisms. In: Methods in Molecular Biology Diagnostic Protocols: PCR and Other Nucleic Acid Methods. Clapp J. (Tatowa, N.J., ed) Humana Pres Inc. pp. 155-175.
Literature cited 2: Cattelan, A.J., Hartel, P.G., and Fuhrmann, J.J. 1999. Screening for plant growth promoting rhizobacteria to promote early soybean growth. Soil. Sci. Soc. Am. J. 63: 1670-1680.
Graham, P., Sadowsky, M., J., and Keyser, H.H. 1991. Proposed minimal standards for the description of new genera and species of root and stem nodulating bacteria. Int J Syst Bacteriol. 41: 582-587.
ID: 59849
Title: SCREENING THE POST DINNER BLOOD PRESSURE CHANGS IN HEALTHY ADULTS BETWEEN THE AGE GROUP OF 19-22.
Author: S.M. Sathak Sameer, P. Manigandan, K. Anusha, M.Chandran and Sekarbabu Hariram
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 299-300 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Vegetarian, Non vegetarian, Dosage, Monitoring.
Abstract: The present study is designed to explore the changes in blood pressure in healthy volunteers (male & female) with the age group of 19-22 after vegetarian/ Non vegetarian dinner. The selected subjects were screened for blood pressure at 5 different periods such as pre, post dinner, zero hour, 4.0 A.M and 7.0 A.M. Volunteers of either sex divided in to 2 groups such as vegetarian and non vegetarian. The results indicates that there is a considerable reduction in blood pressure after dinner both in vegetarian male and female till 4 A.M but in males there is a marginal increase in blood pressure. Regarding Non Vegetarian dinner males shows highly fluctuating blood pressure till 4 A.M whereas in females it shows a steep increase in blood pressure till 4.A.M. The results indicate the significant difference may be due to digestive functioning and the influence of endocrines on the physiology. This type of study will help us to design the dosage schedule of drug for various ailments. It also forms a base for the further researchers to probe the reason for fluctuation in blood pressure.
Location: TE 12 New Biology Building
Literature cited 1: Barr, W. 2000. Lunacy revisited. The influence of the moon on mental health and quality of life. Journal of Psychosocial Nursing and Mental Health Service. 38: 28-35.
Literature cited 2: Zimecki, M. 2006. The lunar cycle. Effects on human and animal behavior and physiology. Pubmed Gov. 60: 1-7.
ID: 59848
Title: COMPARAQTIVE ANALYSIS OF BACTERIOCINS PRODUCED BY PSEUDOMONAS AERUGINOSA AND BACILLUS BREVIS.
Author: V.Jeyanthi Kumari.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 295-298 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Bactriocin, Bacillocin, Pyocin.
Abstract: An attempt is made to comparatively analyse the effectiveness of bacteriocins produced by the indigenous soil bacteria namely Pseudomonas aeruginosa and Bacillus brevis against selective pathogens such as Staphylococcus aureus, Streptococcus pyogens, Enterococcus faecalis and Corynebacterium diptheriae by measuring the zone of inhibition. The bacteriocins are bacillocin of B.brevis and pyocin of P. aeruginosa. Both the bacteriocins effectively acted against the pathogens and comparatively pyocin of P.aeruginosa gave better results than bacillocin of B. brevis. But the effectiveness of bacillocin was particularly greater against Corynebacterium diptheriae (20 mm) than the effectiveness of pyocin (10 mm). Also the determination of bacteriocin production in growth curve revealed that the production of bacteriocins started during early exponential growth phase and continued till last stationary phase. Maximum production of bacillocin and pyocin was found at 11 hours of incubation.
Location: TE 12 New Biology Building
Literature cited 1: Bizani, D. and Brandelli. 2000. A. Characterization of a bacteriocin produced by a newly isolated Bacillus sp strain 8A. J. Appl. Microbiol. 93: 512-519.
Buckenhuskes, H.J. 1993. Selection criteria for lactic acid bacteria to be used as a starter culture for various food commodities. FEMS Microbiol. Rev. 12: 253-272.
Literature cited 2: Cleveland, J., Montville, J.T., Ingolf, N. Chikindas, M.L. 2001. Bacteriocins-safe natural antimicrobial agent for food preservation. Int. J. Food Microbiology. 71: 1-20.
Cotter, D.D., Hill, C. and Ross, R.P. 2006. Class distinction for bacteriocins. Nature Reviews Microbiology. 4777-788.
ID: 59847
Title: ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF DUNALIELLA BARDWIL.
Author: A.Jenitta and S.Jayasuriya.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 285-293 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Dunaliella bardwil, Antioxidants, Radical scavenging activity, Polyphenolic compounds.
Abstract: An ideal antioxidant should be readily absorbed and quench free radicals, and chelate redox metals at physiologically relevant levels. The present study is focused on the antioxidant and free radical scavenging properties of Dunaliella bardawil. The objective of the present study is to evaluate the antioxidant activity of D. bardawil using seven methods viz., DPPH radical quenching method, H2O2 scavenging assay, No radical scavenging assay, reducing capacity assay and FTC method, TBA method and Total phenolic content.Ascorbic acid, ?-Tocopherol and vitamin C were used as standards. The methanolic extract of D.bardawil showed strong DPPH radical scavenging activity of 86.72 ? 0.33 % value with IC50 value of 2.25 ? 0.11 ?g/mL and H2O2 scavenging activity of 94.24 ? 1.33 % with IC50 value of 3.88 ? 0.67 ?g/mL, No radical scavenging activity with 63.55? 0.52 % with IC50 value of 3.22 ? 0.28 ?g/mL. The Dunaliella extract with concentrations higher than 4 mg/mL showed a higher reducing power than alpha-tocopherol and BHA.A volume of 800 ?L of 2.5 % linoleic acid in 99.8 % ethanol in a total volume of 20 mL, was found to produce the highest absorbance readings and both showing a slightly reduced absorbance after the 8th day. Maximum generation of lipid hydroperoxides was achieved by incubation at 37? C (body temperature). In Green phase the contents of phenolic compounds (mg/g) in aqueous extracts of D. bardawil was found 482. 18 ? 12.30 mg GAE /g dry wt and in red phase 525 ? 30.19 mg GAE /g dry wt. Dunaliella showed relatively fair antioxidant potential according to FTC and TBA method but comparatively low DPPH radical scavenging activity. From all these observations it can be concluded that, the algal extracts with high level of polyphenolic compounds and ? carotene in Dunaliella show excellent antioxidant activity in vitro systems.
Location: TE 12 New Biology Building
Literature cited 1: Abalde, J.J., Fabregas and Herrero, C. 1991. b-carotene, vitamin C and Vitamin E content of the marine microalgae Dnaliela tertiolecta cultured with different nitrogen sources. Bioresource Tech. 38: 121-5
Balakrishnan, N., Panda, A.B., Raja, N.R., Srivastava, A. and Prathani, R. 2009. The evaluation of nitric oxide scavenging activity of Acalypha indica Linn Root. Asian.J. Research Chem 2: 148-150.
Literature cited 2: Benzie, I.F.F. and Strain, J.J. 1999. Ferric reducing antioxidant function of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology.299: 15-27.
Cai, Y., Luo, Q. and Sun, M. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74: 2157-2184.
ID: 59846
Title: EVALUATION OF PHYTOTOXICITY OF METALIC NANOPARTICLES AGAINST AMARANTHUS RETROFLEXUS.
Author: A.Aruna, S.Karthick Raja Namasivayam and P.Sarath Chandu.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 279-284 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Phytotoxicity, Metallic nanoparticles, Amaranthus retroflexus
Abstract: Nanotechnology is currently employed as a tool to explore the darkest avenues of medical sciences in several ways like imaging, sensing, targeted drug delivery, gene delivery systems and artificial implants. Based on enhanced effectiveness, the new age drugs re-nanoparticles of polymers, metals or ceramics, which can combat conditions like cancer and fight human pathogens like bacteria. Developmental phytotoxicity of NMS is a critical knowledge gap because nanoparticles entering wastewater streams may predominantly be incorporated into sewage sludge and applied to agricultural fields. In the present study, phytotoxicity of chemogenic metallic nanoparticles such as silver, nano zero valent iron and zinc oxide against common weed against Amaranhus retroflexus. Nanoparticles were synthesized by chemical reduction of metal precursor with reducing agent and the synthesized nanoparticles characterized by scanning electron. Among the different nanoparticles treatment, zinc oxide nanoparticles showed distinct herbicidal activity at all the tested concentration followed by silver. The activity of the nano particles was at 30 ?C followed by 40 ? C. Chlorophyll content was highly reduced in all the tested concentration. Phyllosphere microbial population was not recorded in all the nanoparticles treatment.
Location: TE 12 New Biology Building
Literature cited 1: Ahmad, A.P. Mukherjee, S. Senapati, D. Mandal and Sasqv, M. 2005. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf. 8.28: 313-318.
Albrecht, M.A. and Raston, C.L. 2006. Green Chemistry. 8: 417-432.
Literature cited 2: Braga, L.C., A.A., Leite, K.G. Xavier, J.A. Takahashi, M.P Bemquerer and E. Chartone Souza. 2005. Can J. Microbiol. 51: 541-547.
Ganesh, S. and S. Karthick Raja Namasivayam , 2012. Biofilm Inhibitory Effect of Chemogenic Nano Zerovalent Iron Against Biofilm of Clinical Isolate of Staphylococcus aureus. Asian Journal of Chemistry. 24: 5533-5535.
ID: 59845
Title: : EFFECT OF DIFFERENT FACTORS ON THE PROTEOLYTIC ENZYMES ACTIVITY OF LACTOBACILLUS.
Author: P.Burde and A.O.Ingle
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 275-278 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Casein, Protease, Lactobacillus, Sodium chloride, D-glucose.
Abstract: Lactobacilli are isolated from curd samples. They also form part of mouth and alimentary tract of man. Proteolytic system in bacteria contribute to hydrolyse milk proteins and support for their ability to grow in milk and milk products. Casein is a family of phosphoproteins commonly found in mammalian milk. Casein hydrolyzing activity of the isolated bacteria is pH dependent. Also the separately supplemented sodium chloride 0.3 %, glucose 0. 5 % and maltose 0.2 % is found to increase the proteolytic activity significantly.
Location: TE 12 New Biology Building
Literature cited 1: Chouyyok, W., Wongmongkol, N., Siwarungson, N. and Prichnont, S. 2005. Extraction of alkaline protease using an aqueous two phase system from cell free Bacillus subtilis TISTR 25 fermentation broth. Process Biochemistry. 40: 3514-3518.
Gupta, R., Beg O.K. and Lorenz, P. 2002. Bacterial alkaline protease: Molecular approaches and industrial application. Applied Microbial Biotechnology. 59: 15-32.
Literature cited 2: Ljungh, A.B., Wadstrom, A., Torkel (eds). 2009. Lactobacillus Molecular Biology, From genomics to probiotics. Caister Academic Press.
Makarova, K., Slesarey, A., Wolf, Y., Sorokin, A., Mirkin, B., Koonin, E., Pavlova, N. 2006. Comparative genomics of lactic acid bacteria. Proc. Natl.Aca. Sci. USA.
ID: 59844
Title: BIODEGRADATION OF ACID BLUE 113: KINETIC ANALYSIS AND PLACKETT BURMAN SCREENING DESIGN FOR MEDIUM COMPONENTS
Author: A.Karunya, C.Valli Nachiyar, Swetha Sunkar and S. Anuradha Jabasingh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 267-273 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Consortium, Biodegradation, AB 113, Kinetics, Screening.
Abstract: Four different bacterial strains have been isolated from dye containing effluent and are used for the construction of an aerobic consortium CN-1 along with Pseudomonas aeruginosa CLRI BL22 obtained from CLRI. These bacteria were identified as Citrobacter freundii (2 strains), Moraxella osloensis and Pseudomonas aeruginosa using 16 S r RNA analysis. The organisms constituting the bacterial consortium CN-1 have started to degrade AB 113 in their middle lag phase. They exhibited difference only in the rate in which they carry out the dye degradation. The consortium CN-1 showed gradual increase in the rate of degradation which started in the middle lag phase and reached a maximum by 10h following first order reaction with the rate constant, k1 value being 0.152 h-1. With respect to growth, the consortium showed an extended log phase before it reached its stationery phase by 24h. Screening for medium components using Plackett Burman Screening Design indicated dye concentration having larger effect followed by temperature and ammonium nitrate concentration. It can be seen with low concentration of dye and low concentration of glucose, dye decolorization by the consortium was predominating, provided temperature and ammonium nitrate concentration were held high with pH maintained at 9.
Location: TE 12 New Biology Building
Literature cited 1: Acuner, E., and Dilek, F.B. 2004. Treatment of tectilon yellow 2G by Chlorella vulgaris. Process Biochemistry. 39: 623-631.
Chang, J.S., Chou, C., Lin, P.J., Ho, J.Y. and Hu, T.L. 2001. Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola. Water Research. 35: 2841-2850.
Literature cited 2: Cudgar, R.J. 1985. ' Azodyes ' . In Kirk-othmer Encyclopedia Of Chemical Technology vol.3 Kroschwitz, J.I (Ed)., 4th Edition, Wiley, New York. Pp 821-875.
Copley, S.D. 2009. Evolution of efficient pathways for degradation of anthropogenic chemicals. Nature Chemical Biology. 5: 559-566.
ID: 59843
Title: ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF HIGH SALT TOLERANT BACTERIA WITH CMCase ABILITY.
Author: A.E. Ekram, M.P.E. Shaekh, I.Sarker, S.Zaman, Z.Ferdousi, S.M. Shahik, M.S. Islam, M.S. Noore and A.S.M.H.K. Chowdhury.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 259-266 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: CMCase activity, Penaeus monodon, Salt tolerance, Metabolism
Abstract: A single bacterial colony was isolated by plating the aseptically separated crushed-cuticle sample of Penaeus monodon on an agar solidified LB medium, supplemented with 10 g/ 100 mL salt concentration. The bacterium was found to be gram positive, non-motile, aerobic and lactose non-fermenting through morphological and biochemical tests. The optimum culture condition of the isolate was at pH 7.5 with temperature of 30 ? C. The bacteria showed the ability of utilizing arabinose, sucrose, maltose, fructose as carbon sources and viable cell count was 489 x 107 CFU /mL. Isolated bacterium showed citrate metabolism ability, the ability of breaking down the amino acid tryptophan into indole and no catalase activity. The bacterium was resistant to amoxicillin, penicillin and vancomycin and the MIC value against penicillin was 100 mg/ mL. The bacterial colony was also specially characterized for CMCase production activity. The molecular weight of isolated bacterial genomic DNA was above 10 kb with not much smearing. High salt tolerance ability up to 12 g/ 100 mL medium was observed while culturing on different salt concentrations. Purpose of the study was to screen out a potential source of salt concentrations. Purpose of the study was to screen out a potential source of salt tolerant gene with CMCase activity.
Location: TE 12 New Biology Building
Literature cited 1: Amina, A. and Mahgoub, H.S.A.M. 2011. Salt Inducible Proteins and Conjugal Gene Transfer of Halotolerant Staphylococcus Isolated From Salinity Soil. Egypt. J. Genet. Cytol. 40: 263-280.
Andersen, P.A., Kaasen I., Styrvold, O.B., Boulnois, G. and Trom, A.R. 1988. Molecular cloning, physical mapping and expression of the bet gene governing the osmoregulatory choline-glycine pathway of Escherichia coli. J. Gen. Microbio1.134: 1737-1746.
Literature cited 2: Annamalai, N., Thavasi, R., Vijayalakshmi, S. and Balasubramanian, T. 2011. A novel thermostable and halostable carboxymethylcellulase from marine bacterium Bacillus licheniformis AU01. World J Microbiol Biotechnol. 27: 2111-2115.
Bashan, Y., Moreno, M. and Troyo, E., 2000. Growth promotion of the seawater-irrigated oilseed halophyte Salicornia bigelovii inoculated with mangrove rhizosphere bacteria and halotolerant Azospirillum spp. Biol Fertil Soils. 32: 265-272.
ID: 59842
Title: CARBON DIOXIDE FIXATION BY CO-CULTURE GREEN MICROALGAE THROUGH OVERALL VOLUMETRIC MASS TRANSFER COEFFICIENT (KL A) OF CARBON DIOXIDE IN CLOSED SYSTEM.
Author: Astri Rinanti, Edwan Kardena, Dea Indriani Astuti and Kania Dewi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 253-258 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: CO2 removal, Biomitigation, CO2 fixation, Volumetric mass transfer coefficient, Co-culture.
Abstract: Culture conditions are very important to CO2 bio-fixation but its implementation is limited by the poor mass transfer efficiency. The three green microalgae, i.e. Chlorella, Scenedesmus obliquus, and Ankinstrodemus sp were cultured as co-culture in a vertical bubble photobioreactor as closed system, with 160 ?m size diameter of pore membrane sparger to explore the possibility of coupling CO2 biofixation affected the carbon mass transfer process. In this study, overall volumetric mass transfer coefficient (KL a) of CO2 were developed with operational culture conditions at temperatures (? C) of 30, light intensities (lux) of 4000 and photoperiodism (light/dark; hour) of 16/8, with various high concentration (%) CO2 supplied from the bottom of photobioreactor were 0, 2, 5, 7. The result showed that the highest KL a value (h-1) was 0.1419 5% pure CO2 supplied, gas flow rate 8 L/ min during 12 days experiment. While at this condition, the maximum dried biomass (g/mL) was 2.7 (? = 0.38) at 5 % pure CO2. It could be concluded that CO2 removal efficiency (%) affected by the KL a value, whereas the highest CO2 removal efficiency (%) of 49.02 occurred when the KL a value was highest.
Location: TE 12 New Biology Building
Literature cited 1: Becker, E.W. 1995. Microalgae: Biotechnology and Microbiology. Cambridge University Press, Cambridge.
Carvalho, A.P., Meireles L.A. and Malcata, F.X. 2006. Microalgal reactors: a review of enclosed system design and performances. Journal Biotechnology Progress. 6 (22): 1490-1506.
Literature cited 2: Contreras, Edgardo M. 2007. Carbon dioxide stripping in bubbled column. Journal of Industrial Engineering Chemical Resource, American Chemical Society. 46: 6332-6337.
de Morais, M.G. and Costa, J.A.V. 2007. Biofixation of carbon dioxide by Spirulina sp. and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor. Journal of Biotechnology, 129: 439-445.