ID: 50767
Title: Biopreservative Potential of a Broad-Range Pediocin CP2 obtained from Pediococcus acidilactici MTCC 5101
Author: Baljinder Kaur and Praveen P. Balgir
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Pediocin PA-1, Pediocin CP2, Pediococcus acidilactici, Enterococcus faecalis, Bacteriocin
Abstract: A pediocin PA -1 producing natural Pediococcal isolate- Pediococcus acidilactici MTCC 5101 was obtained from chilly pickle. It produced a 3kDa, thermostable pediocin PA-1, designated as pediocin CP2. Twenty four different microbial strains were screened for determining its antimicrobial spectrum. It was found to inhibit food borne pathogens viz. Listeria monocytogenes, Enterococcus faecalis; opportunistic pathogens such as Pseudomonas putida and Pseudomonas aeruginosa; dental caries causing Streptococcus mutans; mucosal infections causing Neisseria mucosa; food spoilage bacteria including Leuconostoc mesenteroides, Clostridium sporogenes; apart from some LAB strains such as Pediococcus acidilactici LB42, P.pentosaceus, Lactobacillus brevis and a fungal strain of Aspergillus flavus. It was further assessed for its biopreservative potential in two model food systems. MIC valules of pediocin required imparting a specific antagonistic effect against E. faecalis and L. monocytogenes were determined in model milk and liquefied egg systems respectively. It was found to effectively inhibit the growth of food borne pathogenic bacteria viz. E.faecalis and Listeria monocytogenes for 120h at 4oC, with an immediate anti-faecal coliform effect. At 25oC inhibitory responses of pediocin CP2 were less effective as compared to storage temperature of 4oC.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50766
Title: Microbial Degradation of Ficus benghalensis Tree Leaves
Author: Prateek Shilpkar, Mayur Shah, Gaurav Gandhi and Urja Pandya
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Microbial degradation , Ficus benghalensis leaves, Co-digestion, Biomethanation
Abstract: Laboratory experiments were conducted at our centre during June 10, 2005 to December 10,2005 to isolate and identify various thermophilic as well as mesophilic cellulolytic organisms (Bacteria, fungi and Actinomycetes) to degrade Ficus benghalensis tree leaves. After identification the organisms were mass cultured and their potential degradation of cellulose, hemicellulose, starch, lignin etc and production of various enzymes viz cellulase, Carboxy Methyl Cellulase (CMCase), Filter Paperase (FPase), b glucosidase and Xylanase were checked. Result shows that combined use of cellulolytic bacteria, fungi and actinomycetes in equal proportion at thermophilic temperature (55 oC) gives highest biodegradability and enzyme production. Co- digestion of banyan leaves with buffalo dung for biomethanation gives significantly higher biogas production than that of buffalo dung alone at 6%total solids content of feeding slurry.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50765
Title: The Effect of Waxing , Fungicide and Hot Water Treatment to Postharvest Disease Severity of Pineapple (Ananas comosus)
Author: K.Vedhambigai, K.P.Chong and J.J.Silip
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Fungicide, Hot water dip, Waxing
Abstract: The study was done to determine the most effective treatment to control postharvest diseases of pneapple (Ananas comosus). Treatments applied were waxing, fungicide and hot water dip treatment. Fruits were visualized to evaluate whether the treatments influence their ripeness. The severity of damage was determined through visualization by naked eyes and also microbial growth on tissues isolated from inner and outer layer of pineapple. The fungus and bacterial growth was determined from the colony that formed on Potato Dextrose Agar (PDA) and Nutrient Agar (NA) media. All treatments helped to control postharvest disease of pineapple. The treatment controlled diseases in inner part of the fruits only but not effective in shell part. Waxing is the most effective treatment to control postharvest diseases in pineapple than both hot water dip treatment and fungicide treatment. Waxing not only controls the diseases but it helps to delay ripening.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50764
Title: Evaluation of Using Enzyme-Linked Immonosorbent Assay (ELISA) for the Determination of PAHs in Wetland and River Waters
Author: P.S.Talebi, S.M. Talebi, A.Abdollahi and S.V. Hossein
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: PAHs in river and wetland waters, ELISA and GC-MS method
Abstract: The possibility of determination of PAHs in river and wetland waters using enzyme-linked immonosorbent assay (ELISA) was investigated. Several water samples were collected from river Zayandeh- Rud and Ghav-Khoone wetland and analyzed by both ELISA and GC-MS methods and the results showed that there is a considerable differences between the PAHs concentrations obtained by the two techniques. The difference between the results obtained by the two methods for wetland water was much less than those obtained for river water. Futher investigation and analysis of individual PAHs in the samples showed that the concentrations of heavier PAHs that ELISA is more sensitive to them are higher in wetland water. However,ELISA is a rapid and less expensive method that can be used for the roughly evaluation of PAHs pollution in water bodies.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50763
Title: Biochemical Characterizations of few Medicinal plants with Reference to Antifibrinolytic Activity
Author: G.Renuga
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Plants extract, Enzymes Hypertrophy, Histopathological studies
Abstract: An attempt was made to understand the effect of plant extract on fibrinlytic properties. Three plants were selected plants such as Garlic, Onion and Ocimum sps and screened for their efficiency in solubilized blood clot. Extracts of selected plants were compared to identify the principle component has efficiency in solubililzed blood clot. Based on this properties garlic extract has been identified as right plant extract which can be used for further experimental studies, which has high efficiency in solubilized blood clot. The garlic extract has active principle component behing such effects could be isolated through the usual separation techniques and characterized. The component identified from garlic extract has antiplatelet action and its effect has been studied by administration of these extract into hypertrophy induced rat model. Cardiac hypertrophy has been monitored heart wt/body wt ratio at different intervals after aortic constriction along with oral administration of garlic extract in rat. Increased levels of marker ezymes such as CPK, LDH and SGOT in the operated animals , showing a slight reduced activity upon garlic extract administration levels again strengthen the view that the garlic extract may reduce the hypertrophy damage that occur in the operated (hypertrophy induced) animals. The score of necrosis and inflammation correlated with the decrease in severity of hypertrophy when treated with extract was observed by histopathological examination.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50762
Title: Biochemical and Cytological Basis fo Toxicity Lesions Produced by Cr(III) in Germinating Seeds of Lentil (Lens culinaris Medic.)
Author: Sanghamitra Nayak, Hemanta Kumar Patra and Siba Prasad Rath
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Lentil (Lens culinaris Medic.), seed germination, Chromium (III) toxicity, Biochemical lesions
Abstract: An experiment was conducted to study the toxicity effects of chromium (III) ions on seed germination, alterations of biochemical variables, mitotic cell cycle and chromosomes in germinated lentil (Lens culinaris Medic.cv.L-4076) seeds. Germination rate of lentil seeds was decreased gradually with the increase in Cr(III) concentrations. Germination was found to be inhibited with increasing supply of Cr(III) concentrations (expect at 0.001mM), leading to complete inhibition at 30.0 mM chromium supply. The increasing supply of Cr(III) ions also induced various biochemical lesions. During cytological investigation, the mitotic index (MI) gradually decreased with the increase in chromium concentrations which induced genetic damage causing mitotoxic and chromotoxic effects in the treatment materials
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50761
Title: Analysis of Heavy Metals content in Vegetables from Allahbad
Author: S.A.Siddiqui , Wahid Ali, H.S. Gupta and A. Chattree
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Heavy metals, Vegetables, Cadmium, Chromium , Nickel
Abstract: Heavy metal contents in food have become a consumer safety issue. The consumer has the right to know how much heavy metals get incorporated in the food he eats.A bioassay technique was used to investigate heavy Metals uptake by five selected vegetables vig. Radish (Raphanus sativus), beet (Beeta vulgaris), Turnip (Brassica rapa), carrot (Daucus caroto) and spinach (Spinaca oleracia) grown in the vicinity of Allahabad.The aim of present investigation is studying accumulation of heavy metals in green vegetables of Allahabad. The distribution of heavy metals (Co, Cd,Cu, Cr, Zn, Hg, Ag, As, Ni, Mn and Pb) in the green vegetables was estimated at an interval of one month for a period of one year. Which ranged from 0.02mg/kg to 8.92 mg/kg in overall analysis heavy metal contents in the green vegetables were higher with Cu, As and Hg and Lasser with Zn, Cr, Ni and Mn. There was significant correlation between the heavy metals content in vegetables grown in Allahabad. This was attributed to soil properties, climatic condition and translocation with in the plants.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50760
Title: In Vitro Regeneration and Mass Multiplication of Andrographis affinis Nees- An Endemic Medicinal Species from India
Author: S.Govindaraj and C.Alagesaboopathi
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Endemic, MS medium, Andrographis affinis, Tissue culture
Abstract: An efficient protocol has been developed for plant regeneration form nodal explants of vitro grown Andrographis affinis Nees. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with various concentrations of BA, Kn either singly or in various combinations. The highest shoot regeneration frequency (100%) were obtained from nodal segment on MS medium fortified with 2mg/L BA and 1mg/L Kn. The regenerated shoots best on MS 2mg/ L IBA. Regenerated plantlets with well-developed shoot and roots were hardened, successfully transferred to soil and maintained in green house. The present In vitro procedure can be used in conservation and mass multiplication of this endemic medicine plant.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50759
Title: Karyotyping Studies of Traffic Workers Exposed to Auto Mobile Exhaust
Author: T.Yasodha, P.A. Balakumaran and M.Harikumar
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Genotoxicity, Karyotyping, Blood Cell Counts, Peripheral blood, Culture, Double monosomy
Abstract: Genotoxic effects of various chemicals and other toxic substances on workers exposed at their occupational settings, is noteworthy. This exposure results in damage of DNA, thus affecting its replicating ability. Its ability to carry information is also altered Clonefero, 1997. Cytogenetic testing of groups with known or suspected chemical exposure may reveal effect on genetic material on exposed individual. The present investigation focuses on geno-toxic effects in traffic workers exposed to automobile exhausts.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50758
Title: Serological Study on Prevalence of Human Brucellosis in Karnataka
Author: Annapurna S.Agasthya, Shrikrishna Isloor and K. Prabhudas
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Brucellosis, Zoonoses, Indirect ELISA, Sero-epidemiology, Pyrexia of unknown origin cases
Abstract: Brucellosis is a zoonotic disease caused by different species of Brucella. The present work was done at Project Directorate on Animal Disease Monitoring and Surveillance, Bangalore from 2004-2007 to study the sero-prevalence of human brucellosis by using the conventional Rose Bengal Plate agglutination Test (RBPT), Standard Tube Agglutination Test (STAT) and an indigenously standardised Indirect Enzyme Linked Immunosorbent Assay (ELISA). The study group comprised of 2770 samplses which included 618 individuals with a history of animal contact (Group 1), 1500 cases of Pyrexia of Unknown Origin (Group 2) and 652 individuals (Group 3) without a history of fever but with consistent joint pain, backache etc. The analysis of 2770 serum samples by RBPT and STAT revealed 4.98% (138 positives) sero-prevalence and indirect ELISA revealed 11.58% (321 positives) sero-prevalence. All the sero-positive cases were distributed among 10 to 60 years age group individuals . The highest incidence (38%) was found among 41-50 years age group (122 out of 321) followed by 119 out of 321 cases (37.07%) in the age group of 31-40, followed by 46 (14.33%) in the age group of 21-30. Twenty two cases out of 321 (6.85%)were among the age group of 51-60 years. Nine cases out of 321 (2.8%) were among the age group of 11-20 years. The least number of cases i.e. three out of 321 cases (0.93%) were recorded in the age group of <10 years. The indirect ELISA done by using the smooth lipopolysaccharide (S-LPS) antigen of Brucella abortus 99 was found more sensitive than the RBPT and STAT.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50757
Title: Evaluation of Bleach Ammonium Sulphate Sedimentation Method for Preparation of Acid Fast Bacilli smears for Panel Testing and Quality Control
Author: M.Chandrasekar, Sherafin Jancy Vincy and P. Venkatesan
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Panel testing, Bleach Ammonium Sulphate, Sedimentaion, Grade suspension
Abstract: Panel test (PT) slides can be prepared by sodium hydroxide (NaOH) or N-acetyl-L-Cysteine (NALC) sputum digestion method. To acquire the required quality for preparation of PT slides a new improved method is still needed. Bleach (5% sodium hypoclorite) kills the bacilli without altering their morphology and has the ability to digest the mucus. Ammonium Sulphate acts as a strong sedimentation agent. Hence, in the present study, Bleach Ammonium sulphate (BAS) reagent was used for the preparation of panel test (PT) slides. A total of 1250 slides in 10 batches were prepared as per the International guidelines. 300 slides were randomly selected and screened. Statistical analysis of results revealed Standard deviation (SD) for 3+, 2+, 1+, scantly grades as 2, 1, 10, 2 respectively. Mean -2 SD and Mean +2 SD were calculated, when compared with standard consistency table found to be within the limit and TRUE for all 10 batches.These results suggest BAS as alternate suitable sedimentation method for the preparation of PT slides.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50756
Title: How Elevated Levels of Cd, Cu and Pb in the Surface Sediments collected from the Drainage Receiving Metal Industrial Effluents? Comparison with Metal Industrial Drainage and Intertidal Sediments in Selangor , Malaysia
Author: C.K.Yap, M.S.Fairuz and W.H.Cheng
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Metal industrial drainage , Heavy metals , surface sediments
Abstract: Surface sediments were collected in April 2005 from a drainage receiving metal industrial effluents from Serdang in addition to 6 sampling sites from intertidal area and 4 urban drainage sites. The sediment samples were analysed for Cd, Cu and Pb. The metal concentrations at the Serdang industrial drainage sediments were 15.9 ?g/g dw for Cd, 1003.5 ?g /g dw for Cu and 1267 ?g/g dw for Pb. These metal levels were significantly higher than those in the sediments collected from intertidal area (6 sites) and other urban drainages (4 sites) (Cd: 1.39-3.41 ?g /g dw; Cu :6.64- 122.7 ?g /g dw; Pb: 26.0-227.7 ?g /g dw). The total concentrations of Cu, Pb and Cd found at Serdang industrial drainage sediments were 48.76, 23.52 and 8.36 times higher than those in the other sites. Based on the metal concentrations in the four geochemical fractions, for Cu, the EFLE, acid- reducible and oxidisable - organic fractions in the sediment at the Serdang industrial drainage was 59.97, 35.26 and 103.74 times, respectively, higher than those in the other sites. For Pb, the EFLE , acid- reducible and oxidisable -organic fractions in the sediment at the Serdang industrial drainage was 6.17,11.8 and 12.39 times, respectively, higher than those in the other sites. Thus, the elevated concentrations of Cu, Cd and Pb in the sediments collected from Serdang industrial drainage sediments were evidently receiving point source industrial effluent from the nearby metal factory. Therefore treatment on the factory effluent based on the present findings is necessary.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50755
Title: Treatment of Phenol using Anolyte solution generated from the Stel System
Author: Sa ' ari Mustapha, S.T.Zaulfikar, M.Kamil Yusoff and M.Firuz Ramli
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Anolyte, Phenol, Degradation , Oxidation , STEL
Abstract: Anolyte solution generated from the STEL System has been used to treat an aqueous solution of phenol. It noticeably improved oxidation of phenol with a powerful oxidant such as ozone or hydrogen peroxide. Based on the result of the decreasing concentration of phenol and the increasing concentration of carbon dioxide, it was shown that the effect of the anolyte solution obtained. Parameters of study were phenol initial concentration, amount of anolyte, reaction time and pH. Original pH condition of the samples has indicated slightly effect to quantity and quality of the treatment . Up to 43% of phenol removal efficiency could be obtained for 6 hours of reaction time using 6 ml of the anolyte solution. Further addition of anolyte solution and extension of the reaction time, only gave a little decreasing effect to the concentration of phenol.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50754
Title: Impact of Paper Mill Effluent on Groundnut Soil Microflora and Soil Enzymes
Author: P.Dhevagi and G.Oblisami
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Paper mill-Treated waste water, Groundnut, Spermosphere microflora, Soil microflora
Abstract: Use of wastes from industries as soil amendments in agricultural production exemplifies a strategy for converting wastes to resources.The overall objective of this research was to evaluate the effect of treated paper mill effluent along with solid wastes on soil microflora and enzyme activities of groundnut.Effluent irrigation along with amendments increased the physico-chemical and biological properties of the soil. Irrigation with treated effluent increased the beneficial mivroflora like Azospirillum, Azotobacter,Rhizobium, soil enzyme activities and available nutrients.The amylase activity varied from 0.62 to 0.72 and 0.31 to 0.61 mg in the effluent and well water irrigated plots respectively. The CO2 evolved showed a variation of 3.98 to 6.25 mg in effluent irrigated plots and 2.63 to 3.58 mg in well water irrigated plots. The above study revealed that treated paper mill effluent irrigation along with amendments was not harmful to the soil microbial population and its activities rather than uncontrolled release of raw waste into the environment.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 50753
Title: Experimental Aflatoxicosis in Commercial Broilers: Pathomorphological Study of Small Intestine
Author: D.Srinivasa Rao and V.V. Vardhani
Editor: P.K.Wong, R.K.Trivedy, Sadhana Sharma
Year: 2008
Publisher: Asian Journal of Microbiology, Biotechnology & Environmental Sciences (AJMBES) Vol.10, No.2, 2008
Source: Centre for Ecological Science,Indian Institute of Science, Bangalore-12
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology, Environmental Sciences
Keywords: Broilers, Aflatoxin B1, Pathology, Duodenum, Jejunum and Ileum
Abstract: One week (170-180 gm wt.) old broilers wete employed to understand the effect of aflatoxin B1 (AFB1 ) on pathomorphology of small intestine. Two experimental groups treated with AFB1; A (0.01ng/bird) and B(0.1ng/bird) and another control group C were used in the present study. Controls as well as AFB1 treated animals were sacrificed on day 1, 3, 8 and 11 of experimental period and pieces of duodenum, jejunum and ileum were collected, processed, sectioned 95 to 6m) and strained (H&E Method) for histopathological study. It is interesting to note that AFB1
induced marked pathological changes in duodenum, jejunum and ileum of treated animals throughout the experimental period.
Location: 241
Literature cited 1: None
Literature cited 2: None