Enter records

ID: 59940
Title: Virulence marker based multiplex PCR for detecting internalin C (INL C), Internalin J (INL J) and Listeriolysin o (Hlya) genes in Listeria spp.
Author: Samir Das, S.V.S. Malik, Sameer Shrivatasava, Pradeep Gandhale, Satish Kumar, Shabu Shoukat, Durga P. Das, S.B. Barbuddhe and D.B. Rawool.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 909-915 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Listeria monocytogenes, Internalin C, Listeriolysin O, Peptide and ELISA.
Abstract: A multiplex PCR (mPCR) targeting virulence gene (s) of Listeria spp. i.e, Internalin C (inlC), Internalin J (inlJ) and Listeriolysin O (hlyA) in case of Listeria monocytogenes and inl C in case of Listeria ivanovii was developed and evaluated on standard strains of Listeria spp. as well as isolates (25) of Listeria spp. with known biochemical and pathogenicity profiles based on other in vitro and in-vivo pathogenicity test with an ultimate view to evaluate this mPCR for raid and reliable identification and pathogenecity testing of listeria isolates was done. Out of many known virulence marker, listeriolysin-o encoded by hlyA gene is till date a well recognized one with few errors; here in this study InlC and inlJ genes were also studied in conjunction with hlyA to strengthen the existing hlyA marker. The mPCR showed presence of inlC gene in majority but not all virulent strains of pathogenic listeriae, however, it was also detected in some non-pathogenic L.monocytogenes strains. The same trend had been seen in, inlJ also, which revealed although these genes are linked to virulence but may not be an essential a full proof virulence marker for Listeria spp. However, its combination with other more specific virulence marker (s) need to be evaluated on known positive and negative Listeria strains with known pathogenecity profiles on a large number of Listeria isolates of various kind before drawing any final conclusion.
Location: TE 15 New Biology Building
Literature cited 1: Barbuddhe, S.B. and Malik, S.V.S. 2009. Recent advances in the molecular diagnosis of listeriosis. In: ICAR sponsored winter school on ?Molecular diagnostic techniques for zoonotic and foodborne infections?, Feb.07-27, Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, pp. 191-199. Bierne, H., Sabet, C., Personnic, N. and Cossart P. 2007. Internains: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes.Microbes Infect. 9 (10): 1156-1166.
Literature cited 2: Bikandi, J., San Millan, R., Rementeria, A. and Garaizar, J. 2004. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR and endonuclease restriction. Bioinformatics. 22; 20 (5): 798-799. Chakraborty, T., Hain, T. and Domann, E. 2000. Genome organization and the evolution of the virulence gene locus in Listeria species. Int. J.Med. Microbiol. 290: 167-174.

ID: 59939
Title: Inula Racemosa inhibits quorum sensing in Chromobacterium violaceum and Pseudomonas aeruginosa PA 01
Author: H.S. Vasavi, A.B. Arun and P.D. Rekha.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 903-907 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Inula racemosa, Chromobacterium violaceum, Quorum sensing, Virulence, Pseudomonas aeruginosa.
Abstract: Plants used in traditional medicienes are a promising source of bioactive compounds and some can potentially inhibit bacterial quorum sensing (QS ), a cell-cell signaling mechanism mediated by autoinducers to regulate a range of biological functions including virulence in bacteria. Inhibition of QS offers an alternative strategy for antibiotic mediated bactericidal/bacteristatic approach and believed to reduce the risk for development of resistance. The primary objective of this study was to investigate the anti-QS activity of inula racemosa Hook f., a member of the family asteraceae. Anti-QS activity of the ethanol extract of I. racemosa was determined by biosensor bioassay using Chromobacterium violaceum ATCC 12472 and swarming motility in Pseudomonas aeruginosa PA01 was also determined. The ethanol extract of I.racemosa showed anti-QS activity in biosensor bioassay as indicated by clear turbid halo zone of violacein inhibition in C. violaceum CV026 culture plates. It also showed inhibition of violacein production by more than 80 % in C. violaceum ATCC12472 at 1 mg/ mL concentration and complete inhibition of swarming motility in P.aeruginosa PA01 at 0.1 mg/mL concentration. Meanwhile, the growth of C. violaceum and P. aeruginosa PA01 was not affected significantly at all the tested concentration of I.racemosa ethanol extract. The ability of plants to produce substances that affect QS regulation provides with important tool to manipulate gene expression and control bacterial infections in humans.
Location: TE 15 New Biology Building
Literature cited 1: Changhong, L.Mishrta, A.K., Bing, H.E. and Renxiang, T. 2001. Antimicrobial activites of isoalantolactone, a major sesquiterpene lactone of inula racemosa. Chinese Sci Bulletin. 46: 498-504. Chong, Y.M., Yin, W.F., Ho, C.Y., Mustafa, M.R. Hadi, H.A. and Awang, K. 2010. Malabaricone C from Myristica cinnamomea exhibits anti-quorum sensing activity.J. Nat. Prod. 74: 2261-2264.
Literature cited 2: Choo, J.H., Rukayadi, Y. and Hwang, J.K. 2006. Inhibition of bacterial quorum sensing by vanilla extract. Lett.Appl.Microbiol. 42: 637-641. Cui, X. and Harling, R. 2005. N-acyl-homoserine lactone-mediated quorum sensing blockage, a novel strategy for attenuating pathogenicity of Gram-negative bacterial plant pathogens. European J. Plant Pathol. 111: 327-339.

ID: 59938
Title: Bioprocessing of an Eco-friendly microbial alginate and immobilization of an anticancer enzyme L-Glutaminase.
Author: E.SAM Jeeva Kumar, M.RaviKumar, Zahari Che Dan, S. Marylin Jeyapraya,
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 895-902 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Alginate, L-glutaminase, Immobilization, Biopolymer, Exopolysaccharide, Eco-friendly biopolymer.
Abstract: Seaweeds are the commercial source for the production of alginate, which I having a variety of applications. More precisely, they are used as viscosity enhances in food preparations and also in the field of biotechnology as good immobilizing substance for enzymes and proteins. The extraction of alginate causes considerable damage to ecology. The fish and marine life are interwoven to it in their habitat. Seaweeds are the bottom dweller of the food chain and they detoxify heavy metals such as cadmium and lead which will be a health concern if the products are manufactured out of it. Here we had isolated a soil dwelling microorganism found to be of Azobacter sp., which was potential enough to produce alginate. The parameters for the microbial production were optimized and the yield was significant after optimization. Temperature, pH, aeration and agitation of the fermentation medium were studied and optimized. The viscosity of the produced alginate of the fermentation medium were studied and optimized. The viscosity of the produced alginate was quiet appealing at all stages of the production. The strain yielded a maximum of 6.47 mg/mL of alginate. Microbial L-Glutaminase, an anticancer enzyme was selected for immobilization. The alginate produced was of good quality and the enzyme activity was steady for about 72 hours. The kinetics of the enzyme was studied using non-linear regression equation. Michelis Menten and lineweaver-burks plot were made. The enzyme Km and Vmax were found to be 459.2 mM and 1.383 mM/min respectively. The enzyme was found to have a high affinity towards its substrate.
Location: TE 15 New Biology Building
Literature cited 1: Adriana Navarro da Silva and Crispin Humberto Garcia-Cruz, 2010. Biopolymers by Azotobacter vinelandii. Department of Food Engineering and Technology, IBILCE/UNESP. Aravinth Vijay Jesuraj, S., Marylin Jeya Praya, S., BinoKingsley, R., Dinesh Kumar, L. and Ravikumar, M. 2013. Bioprocessing of AR isolates for economical production of L-Glutaminase by solid state fermentation. International Journal of ChemTech Research. 5 (4): 1428-1436.
Literature cited 2: Arruda, L.M.O and Vitolo, M. 1999. Characterization of invertase entrapped into calcium alginate beads. Applied Biochemistry and Biotechnology. 81: 23-34. Aquianti, L., Favilli, F. and Clementi, F. 2004. Comparison of different starategies for isolation and preliminary identification of Azotobacter from soil samples. Soil Biology & Biochemistry.

ID: 59937
Title: Susceptibility profile of bacteria isolated from diabetic wound to silver nanoparticles synthesized using Murraya Koenigii.
Author: S. Anantha Suganiya and R.Mala.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 887-894 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Diabetic wound, Multi drug resistance, Pseudomonas, Murraya koenigii, Silver nanoparticles.
Abstract: The major problem of wound management and therapy is bacterial infection and wound sepsis, which is worst case may, lead to mortality. Proper management of wound and successful wound healing requires the identity of colonizing bacteria and its sensitivity to antibiotics , as most of the pathogens have developed resistance to commonly used antibiotics. Hence, the present work is aimed to evaluate the prevalence, etiology and susceptibility pattern of bacterial pathogens isolated from diabetic wounds to commonly used antibiotics and silver nanoparticles. The sample was collected from Government Hospital in Aruppukkottai, Virudhunagar District, Tamilnadu, India. Silver Nanoparticles were synthesized using Murraya Koenigii. Out of 5 pathogenic isolates, predominant pathogen (3) was Pseudomonas aeurogenosa. Others were Enterobacter sp and Staphylococcus sp. Antimicrobial susceptibility test for isolates were executed using disk and well diffusion method. Pathogens were resistant to gentamycine, neomycine, cephalosporin, penicillin, metrogel, silvergel and ciprofloxacin. But sensitivity was intermediate to ciprofloxacin and susceptible to Silver Nano particles. Nano particles were characterized by XRD and FTIR. MIC of Silver nano particles (SNP) to the isolated P.aeruginosa ASM 100 was 1.5 mg/mL and MBC was 6mg/mL. So the present study reveals that Pseudomonas was highly resistant to most of the drugs used to treat infection. Susceptibility towards silver nano particles opens a window foe new wound dressing with SNP synthesized using edible leaf.
Location: TE 15 New Biology Building
Literature cited 1: Alexander, M.F. 1994. Wound Infection. In: Nursing Practice Hospital and Home, The Adult.Edited by Margaret F. Alexander, Josephine N.Fawcett, Phyllis J. Runciman.Pp.703, Churchill Livingstone, New York. Amro, N.A., Kotra, L.P., Wadu-Mesthrige, K., Bulychev, A.Mobashery, S. and Liu, G. 2000. High-resolution atomic force microscopy studies of the Escherichia coli outer membrane: structural basis for permeability.Langm. 16, 2789-2796.
Literature cited 2: Andrews, J. 2001. Determination of minimum inhibitory concentrations.J.Antimicrob.Chemother.48 (Suppl 1): 5-16 Baram-Pinto, D., Shukla, S.Perkas, N., Gedanken, A. and Sarid, R. 2009. Inhibition of herpes simplex vitrus type 1 infection by silver nanoparticles capped with mercaptoethane sulfonate.Bioconjug.Chem.20: 1497-1502.

ID: 59936
Title: Callus induction and plant regeneration from adventitious buds of ginger (Zingiber officinale Rosc.)
Author: Neeta Shivakumar, A. Pushpa and S. Laxmi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 881-885 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Humnabad, Zingiber officinale, Plant regeneration, Callus.
Abstract: Young buds of Zingiber officanale (Rosc) Cv. Humnabad (a variety grown in Northern Karnataka, India) were used for the initiation of callus growth on MS medium supplemented with various concentrations of NAA and BAP. The percentage of callus formation and average weight of callus were obtained from young buds cultured on medium supplemented with MS +1 mg/L NAA. Shoots were successfully regenerated on the medium with concentrations of MS + 1mg/L NAA+0.1 mg/1 BAP. The rooted shoots were successfully acclimatized in the green house conditions.
Location: TE 15 New Biology Building
Literature cited 1: Anwar, H., Lutful, H., Abdul, K.P., Sultan, M., Ahmad, S. D., Shah, A.H. and Fahrat, B. 2010. Establishment of a suitable and reproducible protocol for in vitro regeneration of ginger (Zingiber officinale Rosc). Pak.J.Bot. 42 (2): 1065-1074. Babu, K.N., Samsudeen, K. and Ratnambal, M.J. 1992. In vitro plant regeneration from leaf-derived callus in ginger. Plant Cell, Tiss Cul. 29: 71-74.
Literature cited 2: De Lange, J.H., Willers, P. and Nel, M. 1987. Elimination of nematodes from ginger (Zingiber officinale Rosc). By tissue culture. J. Horti. Sci., 62: 249-252. Geetha, S.P., Manjula, C., Jhon, C.Z., Minoo, D., Nirmal Babu, K. and Ravindran, P.N. 1977. Micropropagation of Kaempferia sp. (K. Galanga and K. R otunda). J. Spice Arom. Corps. 6 (2): 129-135.

ID: 59935
Title: Production of Alkaline protease enzymes from marine actinomycetes and its antimicrobial and anti-inflammatory studies using mice model.
Author: T.S. Blessy Thayalin and V. Prabakaran
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 875-879 ( 2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Alkaline protease, Anti-inflammation, Streptomyces, Antimicrobial activity.
Abstract: Actinomycetes are gram positive bacteria comprising a group of unicellular microorganisms. Streptomyces is a well-known antibiotic producer. Marine actinomycetes have emerged as a rich source of novel compounds. Alkaline protease enzyme was isolated from Streptomycetes which has wide applications. In the present study, Streptomycetes were isolated from sediment samples collected from Mandapam coast, Gulf of Mannar and it was characterized. Antimicrobial activity was determined by disc diffusion method for the isolates MS-1 and MS-2 treated against human pathogens such as Salmonella, Klebsiella pneumonia, E.Coli and P. fluorescence. Ms-1 and MS-2 revelaed highest antibacterial alkaline protease activity compared to other isolates. Anti-inflammatory studies using mice model system were performed. The mice were treated with crude extracts of actinomycetes orally inorder to study anti-inflammation. It was found out that wihin 10 days the mice found to be cured from inflammation after oral supplementation of crude enzyme extract which indicated these bioactive compounds have analgesic properties.
Location: TE 15 New Biology Building
Literature cited 1: Amadi, J.E and Adeniyi, D.O. 2009. Mycotoxin production by fungi isolated from stored grains. African of Biotech. 8 (7): 1219-1221. Ahmad, S.K. and Singh, P.L. 1991. Mycofloral changes and aflatoxin contamination in stored chickpea seeds. Food. Addit. Contam. 8: 723-730.
Literature cited 2: Agarwal, V.K. and Singh, O.V. 1974. Routine testing of crop seeds for Fusarium moniliforme with a selective medium. Seed Sci Res.2: 19-22. Barbosa, L.N., Rall, V.L.M., Fernandes, A.A.H., Ushimaru, P.I., Probst, I.S. and Fernandes, A. 2009. Essential oils against foodborne pathogens and spoilage bacteria in minced meat. Food borne Pathog Dis. 6 (6): 725-728.

ID: 59934
Title: Antagonistic effect of essential oils against pathogenic storage Fungi of Cowpea.
Author: A.K. Sarma, M.R. Devi and A. Nigam.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 867-874(2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Antifungal, Infective mycoflora, Volatile oil, Vigna unguiculata.
Abstract: The effect of essential oil extracted from the clove (Scyzigum aromaticum), eucalyptus (Eucalyptus oblique), ginger (Zingiber officinale) and peppermint (Mentha piperita) were tested under in vitro condition for their inhibitory effect against twenty numbers of pathogenic fungi isolated from one year stored cowpea (Vigna unguiculata ) seeds at different concentrations (0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09 and 0.1 %) The antifungal activities of the oils were assessed by evaluating the percentage of infected grains. It was recorded that tested oils showed inhibitory effect against the pathogenic storage fungi. The fungi were also responded to the concentration of the oil, i.e. high concentration showed high inhibition. The antifungal activity of the essential oils at 0.02, 0.04, 0.06, 0.08 and 0.1 % concentration was also studied under in vivo condition. Results showed that, application of these oils decreases the incidence of pathogenic storage fungi. It was recorded that, number of fungi appeared at 0.1 % concentration was significantly less. Among the essential oils. The strongest antifungal activity was exhibited by ginger oil.
Location: TE 15 New Biology Building
Literature cited 1: Amadi, J.E. and Adeniyi, D.O. 2009. Mycotoxin production by fungi isolated from stored grains. African J of Biotech. 8 (7): 1219-1221. Ahmad, S.K. and Singh, P.L. 1991. Mycofloral changes and aflatoxin contamination in stored chickpea seeds. Food Addit. Contam. 8: 723-730
Literature cited 2: Agarwal, V.K. and Singh, O.V. 1974. Routine testing of crop seeds for Fusarium moniliforme with a selective medium. Seed. Sci. Res. 2: 19-22. Barbosa, L.N., Rall, V.L.M., Fernandes, A.A.H., Ushimaru, P.I., Probst, I.S. and Fernandes, A.2009. Essential oils against foodborne pathogens and spoilage bacteria in minced meat. Food borne Pathog Dis. 6 (6): 725-728.

ID: 59933
Title: Optimum dose of coagulant for natural surface waters.
Author: A.Khajababau and S.K.Gupta
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 859-866 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Water treatment, Coagulation and flocculation, Jar test, Surface water sources, Turbidity measurements.
Abstract: Conventionally in a water treatment plant fine suspended matter is made to agglomerate into larger size flocs by adding chemicals that neutralize the inherent negative charges on colloidal impurities that repel them from each other and interface with their settling mechanism. It is important that the exact dosage of the chemical (coagulant) is determined prior to addition, because if the dosage becomes more than the particles acquire net positive charge resulting in their repulsion from each other and non-settlement. In water treatment plant, optimum dose of coagulant is generally evaluated by jar test method. Limitation of this test is that it is time consuming, expensive, and the accuracy of results depends upon experience of the plant operator. Coagulant doses are often approximated, resulting in poor quality effluent from secondary settling tanks. In this study water samples were collected locally from different sources e.g. lake, river, village pond a canal. A review of the commonly available coagulants was made and Alum was identified as the potential coagulant for this study. Optimum alum dose tests were carried out on all samples and the results have been analyzed and presented in graphical format to give some idea to plant operator on the approximate values of optimum alum dosages for various sources of water in the region. It is hoped that this study shall reduce the time, effort and costs involved in finding the optimum alum dosages for these surface waters, because the operator can lay his/her hands on rough value as a starting point and then carry out the experimentation to refine his/her results. The study aims to reduce time, cost and effort in operation and maintenance of water treatment plants where turbidity values of the incoming water fluctuate dynamically with time, and offers a sustainable outcome to this issue.
Location: TE 15 New Biology Building
Literature cited 1: Black, A.P. and Chen, C.L. 1965. Electrophoretic studies of coagulation and flocculation of river sediment suspensions with aluminum sulfate. Journal of American Water Works Association. 57 (3): 354-362. John, S.A.A. 1988. Using Moringa seed as coagulants in developing countries.J Am Water Works Assoc.80: 43-50.
Literature cited 2: Lin, S.D. and Evans, R.L.(Personal communication). Physical, chemical and biological changes in water treatment. Illinois State Water Survey, unpublished data. O ' Melia, C.R. and Stumm, W. 1967 .Aggregation of silica dispersions by iron (111) Journal of Colloid and Interface Science. 23 (3): 437-447.

ID: 59932
Title: Effect of pesticide on metamorphosis of frog tadpole, Euphlyctis cynophlyctis.
Author: V.I. Kalamade.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 855-858 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Amphibian, Bioindicators, Pesticide, Hamla, Insect pest, Histological.
Abstract: Decline in the amphibian population has been noted since 1980s from all over the world. Thirty two percent of species are globally threatened nine to twelve species have become extinct since 1980s. This decline is perceived as one of the most critical threats to global biodiversity and environmental imbalance. Several causes are believed to be involved, including diseases, habitat destruction and modification, exploitation, pollution, pesticide use, climate change and increased UV radiation. However many of the causes of amphibian decline are still poorly understood. Contamination of environmental factors by pollutants have resulted in alarming decline in Amphibian as a bioindicators of environment factors by pollutants have resulted in alarming decline in Amphibian population, this fact has highlighted the importance of using Amphibians as a bioindicators of environmental pollution, this fact has highlighted the importance of using Amphibians species breed in rice field, but the pesticide Hamla (Trade name), which is the mixture of Chlorpyrifos and cypermethane, is sprayed to eradicate insect pest on rice. This may contaminate the breeding water to the harmful level. The present work had been carried out to study effect of the pesticide Hamla on metamorphosis of tadpole of Frog (Euphylyctis cynophlyctis) and on histological structure of intestine of tadpole treated with sub lethal levels of Hamla. Our results showed that sub lethal concentrations of Hamla arrested metamorphosis of tadpole and damaged the histological structure of intestine. It appears Hamla is one of the chemical agents responsible for alarming decline in Amphibian population.
Location: TE 15 New Biology Building
Literature cited 1: Cowman, D.F. and Mazanti, L.E. 2000. Ecitoxicology of new generation pesticides to Amphibians. In: Sparlinng, D.W., Linder, G., Bishop, C.A. (Eds) Ecotoxicology of Amphibians and Reptiles: SETAC Press, Pensacola, F.L, 233-268. Davidson, C. 2004. Declining downwind: Amphibian population declines in California and historical pesticide use. Ecological Applications. 14: 1892-1902.
Literature cited 2: Elimann, G.L., Courtney, K.D., Andres Jr., V. And Featherstone, R.M., 1961. A new and rapid colorometric determination of Acetylcholine-esterase metric determination of Acetylcholine-esterase activity. Biochemistry and pharmacology 7: 88-95. Fellers, G.M., McConnell, L.L., Preatt, D and Datta, S. 2003. Pesticides in mountain yellow-legged frogs (Rana mucosa) from the Sierra Nevada mountrains of California USA. Environmental Toxicology and Chemistry. 20: 179-184 (2000).

ID: 59931
Title: Molecular identity for the elite rice variety, Samba Mahsuri (BPT 5204) using SSR markers.
Author: P. Sravanthi, CH.V.Durga Rani, S. Nagalakshmi and M. Madhavi.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 847-854 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Samba Mahusuri, Rice (Oryza sativa L.)
Abstract: The investigation for genetic purity testing of Samba Mahusuri in rice (Oryza sativa L) using microsatellite markers was conducted to identify the signature markers to Samba Mahusuri and also to replace grow out test, which has been used conventionally for seed purity testing through DNA based assays. 40 SSR markers were used to screen the samba mahsuri (BPT 5204). About 38 popular rice varieties including Samba Mahsuri (BPT5204) were used in the present study. And SSR marker RM 11278 could efficiently used to generate molecular IDs for samba Mahsuri BPT 5204 respectively.
Location: TE 15 New Biology Building
Literature cited 1: Arunakumara, K., Durga Rani, Ch. V., Sivaramakrishnan, S., Sundaram, R.M and Vanisree, S. 2008. Assessment of genetic purity in rice cultivar, Samba Mahsuri (BPT 5204) using SSR markers.M.sc Ag (thesis) ANGRAU (Acharya N.G. Ranga Agricultural University ). Botstein, D., White, R.L., Skolnick, M and Davis, R.W. 1980. Construction of genetic linkage map in man using restriction fragment length polymorphism (RFLP). American Journal of Human Genetics. 32: 314-331.
Literature cited 2: Byerlee, D. 1996. Knowledge-intensive crop management technologies: Concepts, impacts and prospects in Asian agriculture. International Rice Research Conference, Bangkok, Thailand. Causse, M.A., Fulton, T.M., Cho, Y.G., Ahn, S.N., Ronald, P.C., Harrington, S.E., Second, G., McCouch, S.R. and Tanksley, S.D. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics.138: 1251-1274.

ID: 59930
Title: Experimental investigation of enhanced production of pectinase by immobilized Aspergillus Niger using low cost substrate.
Author: N.Kavitha, V.Sudarshana Deepa, T. Chitra Devi and R. Ravikumar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 841-846 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pectinase, Immobilized Aspergillus niger, Carrot, Corn, Submerged fermentation.
Abstract: The present study investigated the production of pectinase by immobilized Aspergillus niger NFCCI 2822 in submerged fermentation using low cost substrates of carrot and corn. Batch experiment was conducted at 30 ? C with various substrate concentrations at pH 4.0 with different time intervals to obtain the maximum yield of Pectinase. Results revealed that the medium containing carrot medium which was partially purified had the maximum enzyme activity of 147.02 U/mL than enzyme produced by the corn medium of 103.68 U/mL.Adinarayana, K., Jyothi, B. and Ellaih, P. 2005. Production of alkaline protease with immobilized cells of Bacillus subtilis PE-11 in various matrices by entrapment technique. AAPS Pharmaceutical Science and Technology. 6 (3): 391-397.
Location: TE 15 New Biology Building
Literature cited 1: Adinarayana, K., Jyothi, B. and Ellaih, P. 2005. Production of alkaline protease with immobilized cells of Bacilus subtilis PE-11 in various matrices by entrapment technique. AAPS Pharmaceutical Science and Technology. 6 (3): 391-397. Aguilar, G. and Huirton, C. 1990. Constitutive exopectinase produced by Aspergillus sp. CH-Y-1043 on different carbohydrate source. Biotechnology Letters. 12: 655-660.
Literature cited 2: Akhter, N., Alam Morshed, M.A., Uddin, A., Begum, F., Sultan, T. and Azad, A.K. 2011. Production of Pectinase by Aspergillus niger Cultured in Solid State Media. International Journal of Biosciences. 1 (1): 33-42. Anna, V., Nigar, B. and Venko, B. 2013. Cyclodextrin glucanotransferase production by free and agar gel immobilized cells of B. circulans ATCC 21783. Process Biochemistry. 38: 1585-1591.

ID: 59929
Title: Impact of Environmental acidic pH on oxidative enzymes of fresh water fish, Cyprinus carpio (L)
Author: U.Srineetha, M. Venkata Reedy and M. Bhaskar.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 835-839 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Acidic pH, Gill, Brain, Oxidative enzymes, Cyprinus carpio (L)
Abstract: In the present study the activities of oxidative enzymes like glucose-6-phosphate dehydrogenase (G-6-PDH), lactate dehydrogenase (LDH), glutamate dehydragenase (GDH), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) in gill and brain of Cyprinus carpio at 7th and 15th days of exposure to sub lethal concentration of acidic media (PH5.0) besides controls, are presented in Table 1 and 2. The activities of G-6-PDH, SDH and MDH decreased at day 7 and 15 in the gill and brain of experimental fish with a corresponding increased in the activities of LDH and GDH of experimental fish over control. But in between the two organs the degree of decrease was greater in gill than the brain of fish. Probably, more suppression of oxidative metabolism occurs in gills than the brain of fish. Interestingly, in the case of GDH activity was high in brain than the gill of fish.
Location: TE 15 New Biology Building
Literature cited 1: Bhaskar, M. and Govindappa, S. 1986. Effect of environmental activity and alkalinity on the physiology of Tilapia mossambica (Peters) during acclimation. Biochemical Systematics Ecology. 14: 439-443. Dheer, J.M.S., Dheer, T.R .and Mahajan, C.S. 1987.Haematological and haematopietic response to aid stress in an air breathing freshwater fish, Channa punctatus (Block). J. Fish. Biol. 26: 475-481.
Literature cited 2: Ghillebeart, F., Chaillou, C., Deschamps, F. and Rouboud, P. 1995. Toxic effects at three pH levels of two reference molecules on common carp embryo. Ecotoxicol Environ.Safe. 32: 19-28. Javed, M.H. Yousuf, F.A. Hussain, A.N., Ishaq, M. and Waqar, M.A. 1995. Purification and properties of LDH from liver of uromastix hardwickii. Comp. Biochem.Physiol. 11 (1B): 27-34.

ID: 59928
Title: Study of genomics data for developmental stage of shoot in Arabidopsis Thaliana using principal component analysis.
Author: Nitesh Chandra Mishra, Sunny Patel, Manish Sharma and Kiran Singh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 827-834 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Arabidopsis thaliana, K-means, Microarray, PCA.
Abstract: Time is moving for open and difficult challenges of survival process of living being in nature. We think everything about life depends on energy as nothing is beyond energy limits. Now this study is showing that High throughput gene expression analysis of shoot development in Arabidoopsis thaliana is becoming more and more important in many areas of biomedical research like functional genomics such as gene expression data analysis. cDNA microarray technology is one very promising approach for high throughout analysis and provides the opportunity to study gene expression patterns on a genomic scale. Thousands or even tens of thousands of genes can be spotted on a microscope slide and relative expression levels of each gene can be determined by measuring the fluorescence intensity of labeled mRNA hybridized to the arrays. Beyond simple discrimination of differentially expressed genes or diagnostic classification requires the clustering of genes from multiple experiments into groups with similar expression patterns. This information aims to identify relationships between the structural features, at the molecular level, of gene or gene products that predict the activity of the compound against the cell.
Location: TE 15 New Biology Building
Literature cited 1: Alexander Sturn 2000. Cluster Analysis for large Scale Gene Expression Studies. Arthur, D., Manthey, B. and Roeglin, H. 2009. K-means has polynomial smoothed complexity. Proceedings of the 50th Symposium on Foundations of Computer Science (FOCS).
Literature cited 2: Boyes, et al., 2001. The plant Cell.1499-1510. Clough, S.J. and Bent, A.F. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16 (6): 735-743. Doi: 10.1046/j.1365-313x.1998.00343.x.PMID 10069079.

ID: 59927
Title: Isolation and production of biosurfactant from marine Bacteria.
Author: A. Jayachitra, A. Soma Prabha, T.S. Blessy Thayalin and V. Prabakaran.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 819-825 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Biosurfactants, Bacillus Sp, Emulsification, Hemolysis, Oil displacement.
Abstract: Surfactants, surface active amphiphilic molecules, have a broad spectrum of applications, from everyday tasks such as washing the dishes to advanced oil recovery operations. Biosurfactants are amphiphilic compounds which are produced on living surfaces, mainly on surfaces of microorganisms or may be also secreted extra cellularly and it contains both hydrophilic and hydrophobic moieties which reduces the surface and interfacial tension of the surface and interface respectively. The present study demonstrated the isolation and characterization of biosurfactant producing microorganisms from sea water and sediment samples. The screening of biosurfactant producing microorganisms was carried out employing different parameters such as surface activity and emulsification activity. Our study revealed strain S2 and S3 showed positive results. Oil displacement test and Haemolytic activity revealed an enhanced activity. On analysis with emulsification activity with petrol as substrate it was found out that an increased emulsion with 72 % was recorded against Bacillus subtilis (S3) and followed by (S2) Bacillus cereus. Besides, on carbon sources tested as glucose, sucrose, petrol exhibited efficient biosurfactant production. An optimum pH of 8 was found better for surfactant production. The crude surfactant production from Bacillus obtained from marine samples were tested for antibacterial activity against pathogens Klebsiella pneumonia, Pseudomonas aeruginosa, Pseudomonas fluorescence and E.coli were found effective in inhibition of pathogens.
Location: TE 15 New Biology Building
Literature cited 1: Benincasa, M., Contiero, J., Manresa, M.A. and Morase, J.O. 2002. Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soap stock as the sale carbon sources. Journal of food Engineering. 54: 283-288. Cooper, D.G. and Goldenberg, B.G. 1987. Surface-active agents from two Bacillus species. Appl Environ Microbiol. 53 (2): 224-229.
Literature cited 2: Desai, J.D. and Banat, I.M. 1997. Microbial production of surfactants and their commercial potential. Microbiology and Molecular Biology Reviews. 61 (1): 47-64. Haddad, N.L.A., Wang, J.and Mu, B. 2009. Identification of a surfactant producing strain: Bacillus subtilis HOB2.Prot Pept Lett.16: 7-13.

ID: 59926
Title: Biodegradation of complex hydrocarbon compounds by soil isolates of Dehradun region, India.
Author: Kunal Madhav, Neha Tanwar, Nupur Gusain, Latika Sharma and Aditya Chaudhary.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (4) 811-817 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Naphthalene, Biodegradation, Paracocuus spp., Paenibacillus spp.
Abstract: PAH like naphthalene are so toxic there is interest in understanding the physicochemical processes and microbial degradation reactions that affect the mobility and fate of these compounds in groundwater and soil sediment system. The present work focuses on Biodegradation of petroleum Hydrocarbons by bacterial soil isolates of soil samples were collected in sterile containers from petrol pumps. The soil sample from selected sites were diluted and plated on sterile NAM plates. Colonies of Bacillus spp., Klebsiella spp., Paracoccus spp. and Paenibacillus spp. were obtained. The isolates obtained were then tested against Petrol, Diesel, Lubricating oil and Naphthalene which were incorporated in Basalt Salt Media (broth and agar) and were incubated at 30 ? C for five days. Readings from the broth culture was recorded every 24 hours at 595 nm for five days. Readings from the broth culture was recorded every hours at 595 nm for five days and the results were depicted graphically. The results obtained were compared with hydrocarbon utilizing Pseudomonas aeruginosa MTCC 2474 strain.
Location: TE 15 New Biology Building
Literature cited 1: Abd-Elasalam, E. Hassan, Hafez, E. Elsayed, Hussain, A. Azhar, Ali G. Amnay and El-Hanafy, Amr, 2009. Isolation and identification of three-rings polyaromatic hydrocarbons (anthracene and phenanthracene) degrading bacteria. American Journal of Agriculture and Environmental Science5 (1): 31-33. Alquati Claudia, Papacchini Maddalena, Riccardi Carmela, Spicagelea Sergio and Besttteti Giuseppinea, 2005. Diversity of Napthelene-degrading bacteria from a petroleum contaminated soil. Annuals of Microbiology. 55 (4): 237-242.
Literature cited 2: Bist Sandeep, Pandey Piyush, Sood Anchal, Sharma Shivesh and Bisht, N.S. 2010. Biodegradation of Napthelene and anthracene by chemo-tachtically rhizobacteria of Populus Deltoids. Brazalian Journal of Microbiology. 41: 922-930. Chaudhary Kumar Vivek and Borach Debajit. (2011).Isolation and molecular characterization of hydrocarbon degrading bacteria from tannery effluent. International Journal of Plant, Animal and Environmental Science. 1 (1): 2231-4490.