ID: 55987
Title: Enrichment of protein content of pods and fruits of vegetable plants exposed to different percentages of vermicompost
Author: P K Ramasamy and S Umavathi
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Eudrilus eugeniae, vermicompost, Lady ' s finger (Hibiscus esculentus L). Indian butter bean (Dolichos lab lab L), tomato (Solanum lycopersicum L), pods, fruits, protein content
Abstract: The decline in the nutrient level of agricultural land is an important drawback due to continuous cultivation. In order to increase the soil fertility, inorganic fertilizers are being widely utilized in our cultivable lands. Even though they promote the growth of crops, their toxic effect is the negative impact by means of their over utilization. To overcome all these unwanted factors the application of organic manure especially vermicompost is recommended. Vermicompost is a rich source of macronutrients, micronutrients, vitamins, plant enzymes and plant growth hormones. So, the present investigation was performed to study the influence of vermixompost on protein content in the pods and fruits of Lady ' s finger (Hibiscus esculentus L.), Indian butter bean (Dolichos lab lab L.) and Tomato (Solanum lycopersicum L.). Different percentages of vermicompost (25%, 50%, 75% and 100%) derived from the earthworm, Eudrilus eugeniae was made with red soil. The Lady ' s finger (Hibiscus esculentus L), Indian butter bean (Dolichos lab lab L) and Tomato (Solanum lycopersicum) plants were cultivated in these vermicompost -red soil mixtures for 90 days period of exposure. At the end of 90 days the pods of Lady ' s finger and Indian butter bean and fruits of the tomato plant were collected and tested for the level of the protein content. In the present study, high level of protein content was noticed in the pods of the Lady ' s finger and fruits of the Tomato plants grown in 50% vermicompost concentration but at the same time the high level of protein was noticed in pods of Indian butter bean plant cultivated in 75% vermicompost concentration. However, the low level of protein was noticed in the pods of Lady ' s finger and Indian butter bean and in the fruits of tje Tomato from control plants of both plants.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55986
Title: Effect of vermiwash on laccase enzyme production by the newly isolated fungal isolate SMWF-10
Author: D Raghunathan and Kantha D Arunachalam
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Laccase, vermiwash, ABTS
Abstract: None
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55985
Title: Iron accumulation by Bacillus circulans
Author: K V Pavani, Kiran Kalia and Gayathramma K
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Iron, proteins,Bacillus circulans
Abstract: Biosorption technology has gained tremendous importance because microbes could be the cheapest source of detoxification of effluent streams. The capacity of Bacillus circulans isolated from industrial effluent to grow and concentrate metal from the differet concentration of iron (Ferrous Sulphate) containing medium was studied. Well defined growth of Bacillus circulans was observed only in the medium containing 0.2-4.0 m M of iron. The culture showed considerable inhibition of growth with iron as compared to the metal free controls. The maximum uptake of iron was observed in the cells grown in 3.0 m M concentration and further increase in the metal concentration was found to decrease its uptake. The cell membrane protein profile of the cells grown in metal free media showed the presence of ten different proteins. The total protein expression was reduced by the stress of iron.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55984
Title: AM colonization, nodulation and growth of Sesbania grandiflora tree species in ten social forestry nurseries
Author: Pavan Kumar Pindi
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: AM fungi, nurseries, Sesbania grandiflora, tree species
Abstract: S. grandiflora is a short-lived, quick - growing, soft wooded tree. It is grown for ornaments and is valued as food and also as a good fodder. Bark yields a good fibre and a gum and various parts have medicinal value. Variations in mycorrhizal colonization, nodulation and growth of Sesbania grandiflora tree species was investigated from ten social forestry nurseries. AM Infection and number of resting spores vareid with the type of soil. A direct correlation was observed between the phosphorus uptake with AM infection and nitrogen content with the number of root nodules. Sesbania grandiflora exhibited maximum colonization, spore population and nodulation in Gadwal nursery and minimum in Maktal.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55983
Title: Effect of gamma irradiation on aroma constituents of ginger rhizomes
Author: R Dhanya, B B Mishra and K M Khaleel
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Ginger, shelf-life, gamma radiation, volatile oil, GC-MS
Abstract: Fresh ginger rhizome is an important spice of medicianl and economic important which are prone to spoilage during storage. Gamma irradiation preservation and techniques area used to prolong the shelf life of these rhizomes. Gamma irradiation at the doses of 1, 3 and 5 kGy were carried out and volatile oil constituents of ginger rhizomes were analyzed by GC-MS. No statistical differences in volatile oil constituents were found between the radiation treated and control non-radiation treated rhizomes.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55982
Title: Effectiveness of free and immobilized cells of Aspergillus niger in the production of alpha-amylase from starch
Author: Neelam Gautam, K P Singh and D R Modi
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Aspergillus niger, alpha -amylase, immobilized cells, nitrogen source
Abstract: Alpha- amylase production has a wide range of application in many industries such as baking, brewing, wine and textile etc. The present study is concerned with the characterization of alpha-amylase producing Aspergillus niger isolate, optimization of medium composition, cultural conditions for alpha-amylase production, extraction and partial purification of extra celluar enzyme from a potential isolate, using both free and immobilized cells. The optimum pH, temperature and incubation period recorded for enzyme production were 5.0, 350 C and 5 days for immobilized cells and 5.0, 300 C and 5 days for free cells respectively. Among the carbon sources, starch was observed to be the best for a-amylase production. Peptone at 0.03% was an ideal nitrogen source. However, the surfactants that are Tween-80, Triton X-100 and SDS (Sodium dodecyl sulphate) at 0.02%, 0.002% and 0.0002% concentration respectively were most effective for enhancement of alpha-amylase production. Eventually, the characterization of the enzyme was performed by thin layer chromatography.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55981
Title: Epidemiological survey and PCR based detection of mutation for breast cancer in West Bengal, India
Author: Arpita Chatterjee and Gopeswar Mukherjee
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Breast cancer, rural Bengal, Mutation, BRCA2 gene
Abstract: Carcinoma breast was the most prevalent malignant condition among women world-wide. Present investigation on the incidence pattern of breast cancer in rural population of West Bengal revealed that among 3219 malignant cases 39.05% showed breast carcinoma including 99.20% females and 0.79% males. Cancer frequency was moderate during 2004 to 2006, but it increased dreastically from 2007 and reached maximum peak (28.00%) in 2009. Carcinoma breast was most prevalent in group O (32.86%) and in particularly middle age group (39.46% in 31-40 years age). A PCR based molecular approach was used to identify the mutation of BRCA2 genes and in very few cases (0.8%) BRCA2 999del5 mutation was detected.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55980
Title: Mycobiota and their relative percent incidence on freshly harvested brinjal seeds (Solanum melongena L) produced in Karnataka
Author: K M Jayaramaiah and G R Janardhana
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Mycobiota, percent incidence, solanum melongena, standard blotter method
Abstract: Ten different freshly harvested Brinjal seed varieties (Solanum melongena L) commonly produced in different districts of Karnataka were collected and screened for extent of mycobiota and thier the percent incidence by using standard blotter method. The seed health testing method revealed, at least 41 different species of fungi belonging to 22 genera. The dominant disease causign mycobiota recorded on popular varieties of brinjal include species of Alternaria, Bipolaris, Cercospora, Colletotrichum, Fusarium, Phoma and Phomopsis respectivley. The high incidence of mycoflora was responsible for reduced seed germiantion as evident from low percent seed germination.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55979
Title: Influence of bioinoculants on the growth of Gliricidia maculata in nursery conditions
Author: Pavan Kumar Pindi
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: AM fungi, nurseries, Gliricidia maculata
Abstract: Gliricidia is a small genus of trees and shrubs and native of tropical America. G. maculata is widely cultivated in the tropics as a shade and ornamental tree. Studies on influence of bioinoculants on growth of Gliricidia maculata tree species responded positively towards the different inoculations. However, the response varied with the type of bioinoculant. Dual inoculations of mycorrhizal fungi and Rhizobium have shown better results than the other types. Dual inoculation involving mycorrhizae and Azotobacter, Rhizobium and PSB performed better than single inoculations of mycorrhizae. A mixture of mycorrhizae appears to be better inoculum than the individual AM fungi. AM fungal species varied in thier efficiency in supporting the seedling growth. They further suggested that mixed inoculum might be used more efficiently than single inoculum for field inoculations.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55978
Title: Evaluation of termite mound soil for antiviral property with particular reference to measles and mumps
Author: A Yogamoorthi and Y Valentina
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Termite mound, soil, antiviral, cytotoxicity, MTT assay, measles, mumps
Abstract: Aquous and ethanol extracts of termite mound soi are evaluated for antiviral property using cell viability assay, antiviral assay, MTT assay and cytotoxicity assay. The results revealed that both extracts showed higher level of antiviral property to the tune of 1:256 dilution and highest activity towards measles when compared to its standard. The reason for such pharmacological bioactivity of the soil is discussed with previous reports.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55977
Title: Anti bacterial effect of selected medicianl plant extracts on strains of enterobacteriaceae isolated from sewage water, Visakhapatnam, India
Author: P V D Soujanya Kumari and A Balakrishna
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Medicinal plant extracts, enterobacteriaceae, sensitivity, resistance and violet red bile glucose agar
Abstract: The anti microbial activity of leaf and bark extracts prepared from 5 medicinally important plants like Terminalia alata, Dillenia pentagyna, Xylia xylocarpa, Pterocorpus marsupium and Lannea coromondelica were tested against 22 isolates belonging to the family Enterobacteriaceae by using agar diffusion method. The strains were isolated from sewage sample, collected from sewage treatment plant, Visakhapatnam, by using modified selective VRBG (Violet Red Bile Glucose) agar medium, which allows the recovery of all members of Enterobacteriaceae. The crude extracts prepared methanol showed zone of inhibition ranging from 0.1mm - 15mm. The leaf extract of Terminalia alata was capable of inhibiting maximum number (68%) of strains. The bark extrat of Lannea coromandelica and leaf extract of Dillenia pentagyna were capable of inhibiting 22% & 13% of the strians. The other two extracts were not showing any antibacterial activity. The result indicates that the leaf extract of Terminalia alata possessed potential antimicrobial activity against Enteric strains.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55976
Title: Detection of microbial contamination in foods with advanced techniques
Author: Sadaf Ahmad and Ahmad Ashfaq
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Food contamination, microbes, foreign material, detection techniques
Abstract: The presence of contaminants in food is of major concern to the producer. Mechanical separation techniques have been used for many years for finding foreign bodies in powdered and flowering products on the basis of size and weight. Optical inspection techniques extend the range of detectable foreign objects with regard to shape and colour in free-flowering materials. Metal detectors enable metallic particles inside the body of a product to be found. With advances in sensor technolgies and computing power, more advanced detection systems are becoming available. This paper summarizes the types of food contaminants and approaches to food quality assurance with respect to microbes and foreign body detection.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55975
Title: Antioxidant activity and antioxidant phytochemical analysis of mangrove species Sonneratta alba and Bruguiera cylindrica
Author: Poonam Gawali and B L Jadhav
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Mangroves, ROS, DPPH, reducing power, scavenging activity
Abstract: The antioxidant activity anad antioxidant phytochemicals in the stem and leaves of S. alba and B. cylindrica species of Mumbai coast were studied. Present results have confirmed scavenging free radicals potentials of B. cylindrica and S alba. IC50 values for DPPH radical scavenging activity of stem and leaves of S. alba was 62.5 and 87.5? g/mL while for B. cylindrica it was 162.5 and 175 ?g/mL respectively. The reducing power of all the extract of both the plant increased dose-dependently in which S. alba showed higher reducing power. Among the antioxidant phytochemicals both the plant revealed presence of flavonoids, tannins, triterpenes, anthraquinones, alkaloids and saponins.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55974
Title: Preliminary phytochemical investigation and antimicrobial activity of Sphaeranthus indicus Linn
Author: S Ahmed John and B Tamil Marai Selvi
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: Sphaeranthus indicus, plant extract, phytochemical analysis, antibacterial activity, agar diffusion
Abstract: Leaves, stem and roots of S. indicus were extracted separately with methanol, ethanol, chloroform, petroleum ether and hot water and the extracts were screened for its phytochemical constituents. The plant revealed the presence of alkaloids, saponins, tannins, flavonoids, steroids, terpenoids,cardiac glycosides, aminoacids, monosaccharides and reducing sugar. Leaves extracts showed significant amount of phytochemicals and hence antimicrobial studies of leaves extracts were carried out against bacterial species like Bacillus sp, Staphylococcus sp, Klebsiella sp, E coli, Pseudomonas sp using filter paper and agar well diffusion method at 4 different concentrations. Aqueous extract showed the highest inhibitory effect compared to all other extracts and it showed good inhibitory activity against Bacillus sp followed by Staphylococcus sp. The gram positive bacteria were found to be more susceptible than gram negative bacteria. Antifungal activity of methanol and ethanol extracts were tested against Penicillum sp and Aspergillus sp and the growth was found to be decreased with increasing concentration of the extracts.
Location: 241
Literature cited 1: None
Literature cited 2: None
ID: 55973
Title: Development of an enzyme linked immunosorbent assay for the detection of glyphosate in water samples using avian antibodies
Author: A Arul Selvi, M A Sreenivasa and H K Manonmani
Editor: Dr P K Wong, Dr R K Trivedy, Dr Sadhana Sharma
Year: 2011
Publisher: Global Science Publications, Vol 13, No 2, 2011
Source: Centre for Ecological Sciences
Reference: None
Subject: Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Keywords: ELISA, avian antibodies (IgY), detection of glyphosate, cross reactivity, food samples, specificity
Abstract: A simple competitive immunoassay was developed for the measurement of glyphosate in water samples. The assay employed the avian antibodies (IgY) that recognized glyphosate as a capture reagent and glyphosate - alkaline phosphatase conjugate as an enzyme label. The assay depended on the competitive binding reaction between the antiglyphosate antibody and glyphosate derived from water samples for binding sites with immobilized glyphosate- OVA conjugate. The concentration of glyphosate in the water samples was quantified by the ability of the pesticide present in water samples to inhibit the binding of the enzyme congugate to the antibody and subsequently the colour formation in the assay. The assay was specific to glyphosate with a limit of detection of 2 ng/mL. Mean analytical recovery of glyphosate in different water samples was 42.97 to 160.00%. The precision of the assay was satisfactory. Coefficients of variation were 0.002 to 1.24 and 0.055 to 1.41% for intra and inter assay precision respectively. The assay compared favourably with HPLC in its ability to accurately measure glyphosate in the water samples.
Location: 241
Literature cited 1: None
Literature cited 2: None