Enter records

ID: 59887
Title: RAPID MOLECULAR DETECTION AND IDENTIFICATION OF AFLATOXINS PRODUCINGFUNGAL GENE, AFLQ, IN INFCTED MAIZE SAMPLES USING NORMAL PCR.
Author: Nagalakshmi S, Manorama K, Anurag Chathurvedi, Sreedhar M, Krishna Bhagavatula MR, Sunil-Chidambar K, Ravicharan A, Prathima N, Durgarani Ch. V and Reddy Vln.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 667-671 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Maize, Aflotoxins and PCR
Abstract: Maize (Zea mays L.) is one of the cereals which serves as a main source of food, forage and processed products for industry with a production of around 790 million tones. As a staple food it provides more than one-third of the calories and proteins in some countries. Aflatoxins are a group of chemicals produced by certain mold fungi such as Aspergillus flavus and Aspergillus parasiticus. Aflatoxins are harmful or fatal to livestock and human beings as they are carcinogenic. The present study was undertaken with the main aim of detecting the presence of these genes encoding the production of mycotoxin in the infected maize samples. One hundred and thirty maize samples infected with the fungi Aspergillus parasiticus and Aspergillus flavus were collected from the markets, godowns and farmers ' fields. DNA isolated from such maize samples was amplified in PCR using four sets of forward and reverse gene specific primers designed using DNASTAR Lasergene 8.0 version software from original gene sequences (obtained from GENBANK) of the specific gene, aflq. The fragments obtained were resolved on the bioanalyzer, DNA 1000 Labchip, for generating data on the size of amplified fragments. A 168 base pair fragment of aflq target sequence was amplified in twenty nine maize samples. This study helps in easy detection of mycotoxins present in the contaminated samples in storage which are unfit for consumption thereby avoiding the hazardous influence of such toxins on human and animal health.
Location: TE 12 New Biology Building
Literature cited 1: Bottalico, A. 2009. Aspergillus diseases of cereals: species complex and related mycotoxin profiles, in Europe. Journal of Plant Pathology. 80: 85-103. Niessen, L. 2007. PCR based diagnosis and quantification of mycotoxin fungi. International Journal of Food Microbiology. 119: 38-46.
Literature cited 2: Russell, R. and Paterson, M. 2006. Identification and quantification of mycotoxigenic fungi by PCR. Process Biochemistry. 41 (7): 1467-1474. Strange, R.N. and Scott, P.R. 2006. Plant Disease; a threat to global food security. Annual Review of Phytopathology. 43: 83-116.

ID: 59886
Title: UTILIZATION, ISOLATION AND CHARACTERIZATION OF CHLORELLA VULGARIS FOR CARBON SEQUESTRATION AND WASTE WATER TREATMENT BY PERFORMING FTIR SPECTRAL STUDIES.
Author: P.Indhumathi, M.Soundararajan, P.S. Syed Shabudeen, U.S. Shoba and E. Suresh.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 661-666 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Chlorella vulgaris, uv-visible , FTIR, SEM, Nitrate removal, Phosphate removal.
Abstract: Green microalgae Chlorella vulgaris species was collected from Bhavani Lake at Erode District, Tamil Nadu State, India. The Fourier Transform Infrared spectroscopy and Scanning Electron Microscopic studies were utilized to examine morphology of Chlorella vulgaris is attempted for its efficiency in removing nitrate and phosphate from waste water medium. These experimental studies reveal that, ' Chlorella vulgaris ' is having full-fledged efficiency in removing nitrates and phosphates at a time intravel of 6 days.
Location: TE 12 New Biology Building
Literature cited 1: Anderson, R.A. 2005. Algal Cultural Techniques, Elsevier academic press, London. Becker, W. 2004. Microalgae in human and animal nutrition. In: Richmond, A editor. Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Blackwell Publishing, 312-346.
Literature cited 2: Benning, L.G., Phoenix, V.R., Yee, N. and Tobin, M.J. 2004. Molecular characterization of cynobacterial silification using synchrotron infrared microspectroscopy. Geocimica et Cosmochimica Acta. 68: 729-741. Basterj, Korting, H.C., Traenkle, P. and Schmalreck, A.F. 1999. Identification of dermatophytes by Fourier Transform Infrared Spectroscopy, Mycoses ISSN 09333.42: 525-528.

ID: 59885
Title: IDENTIFICATION OF DRUG-RESISTANT BACTERIA AMONG EXPORT QUALITY SHRIMP SAMPLES IN BANGLADESH.
Author: Tasnia Ahmad, Sagar Baidya, Basudeb Chandra Sharma, Mushfia Malek, Kamalkanta Das, Mrityunjoy Acharjee, Saurab Kishore Munshi and Rashed Noor.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 655-660(2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Export quality shrimp, Pathogens, Antibiotic resistance, Microbiological quality, Consumer safety.
Abstract: Present study attempted to detect the pathogenic load n export quality shrimp samples and to demonstrate the drug resistance traits of the pathogenic isolates. Samples were found to be contaminated with Listeria spp. (1.0 x 105 cfu/g), Staphylococcus aureus (9.1 x 106 cfu/g) and Escheria coli (1.0 x 103 cfu/g). Study of antibiogram revealed that Listeria spp. was resistant against penicillin G and ampicillin, S. aureus against trimethoprim, erythromycin, sulfamethoxazole, and Escherichia coli against erythromycin and polymixin B. However, all the isolates were found to be susceptible against tetracycline, and most of them also exhibited a greater degree of susceptibility towards amoxicillin, ceftriaxone, ciprofloxacine, streptomycin, chloramphenicol and cefixime. The antibacterial activity activity of the shrimp blend was also examined. Interestingly, the head and body portions of the shrimp samples exhibited antibacterial activity against Staphylococcus spp. and Listeria spp.
Location: TE 12 New Biology Building
Literature cited 1: Alfrad, E.B. 2007. Bensons microbiological applications. McGraw-Hill Book Company, New York. Allerberger, F. and Mittermayer, H.2008. Antimicrobial stewardship. Clinical Microbiology and Infection. 14 (3): 197-199.
Literature cited 2: Antony, M.M, Jeyasekaran, G., Shakila, R.J. and Shanmugam, S.A. 2002. Microbiological quality of raw shrimps processed in seafood processing plants of Tuticorin, Tamil Nadu, India. Asian Fisheries Science. 15 (1): 33-41. Bauer, A., W., Kirby, W.M.M., Sherris, J.C. and Tierch, M. 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology. 45 (4) : 493-496.

ID: 59884
Title: MICROBIOLOGICAL STUDY OF DRINKING WATER: QUALITATIVE AND QUANTITATIVE APPROACH.
Author: Tasnia Ahmed, Mrityunjoy Acharjee, Md. Shohanur Rahman, Monirunnessa Meghla, Janifar Jamal, Saurab Kishore Munshi and Rashed Noor.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 647-654 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: MPN (Most Probable Number) Fecal contamination, Indicator bacteria, Pathogens, Drinking water.
Abstract: Consumption of drinking water contaminated with focally originated pathogenic bacteria is mostly responsible for the onset of water borne diseases outbreaks especially in developing countries. Current study attempted to analyze 25 treated drinking water samples both qualitative-and quantitatively from different areas of Dhaka metropolis, Bangladesh where 90 % cases of diseases (dysentery, typhoid, Cholera and diarrhea) have long been reported due to the water borne microorganisms diseases. Through the most probable number (MPN) method, 5 samples out of 25 were found to be non-potable as they had been contaminated with Escherichia coli indicating the risk for fecal contamination responsible for diseases outbreaks. Other contaminating Gram negative bacteria were characterized as Klebsiella spp, Alcaligenes faecalis, Pseudomonas spp. and Aeromonas spp. Intrestingly the presence of E.coli was detected in the same 5 samples within a range of 3.0 x 102 to 1.7 x 105 cfu/ml by the conventional cultural and biochemical methods. Moreover, a huge array of other pathogenic bacteria was also detected (5.0 x 102 -3.5 x 108 cfu/mL) through this method. Further detection of drug resistance traits among the bacterial isolates would be of public health significance.
Location: TE 12 New Biology Building
Literature cited 1: Abera, S., Zeyinudin, A., Kebede, B., Deriew, A., Ali, S.and Zemene, E. 2011.Bacteriological analysis of drinking water sources. Afr. J. Microbiol. Res. 5 (18): 2638-2641. Acharjee, M., Jahan, F., Rahman, F. and Noor, R. 2013. Bacterial proliferation in municipal water supplied in Mirpur locality of Dhaka city, Bangladesh. Clean Soil, Air, Water, DOI: 10. 1002./clen.201200618
Literature cited 2: Acharjee, M., Rahman, F., Beauty, S.A., Feroz, F., Rahman, M.M. and Noor, R. 2012. Microbiological study on supply water and treated water in Dhaka city, S.J.Microbiol. 1 (1): 42-45. Alfrad, E.B. 2007. Bensons Microbiological Applications. Mcgraw-Hill Book Company, New York.

ID: 59883
Title: A REVIEW OF THE EFFECTS OF BALCONY ON INDOOR VENTILATION PERFORMANCE.
Author: Fatemeh Mozaffari Ghadikolaei, Dilshan Remaz Ossen and Mohd Farid Mohamed
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 639-645 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Balcony, Natural ventilation, Multi storey building.
Abstract: Incorporating airflow principles and techniques, and applying them in building designs can create comfortable indoor environments. Fa?ade characteristics, especially near the opening wall, can change airflow patterns and airspeed inside buildings. Balcony is an example of these features that can be used in front of openings to control the performance of indoor ventilation. Through literature review, this paper looks into the effeteness of the provision of balconies to induce natural ventilation in building. This paper reviews several studies on airflow patterns inside and outside buildings in single-sided and cross ventilation strategy as well as the impact of wind incidents, building height and balcony configuration on ventilation performance. The outcome of this study demonstrates that architects can incorporate balcony as a green design element to improve indoor natural ventilation.
Location: TE 12 New Biology Building
Literature cited 1: Ai, ZT, CM Mak, JL Niu, and ZR Li. 2011. The assessment of the performance of balconies using computational fluid dynamics. Building services Engineering Research and Technology 32 (3): 229-243. Ai, ZT, CM Mak, JL Niu, and ZRLi. 2011. Effect of balconies on thermal comfort in wind-induced, naturally ventilated low-rise buildings. BuildingServices Engineering Research and Technology 32 (3): 277-292.
Literature cited 2: Ai, ZT, CM Mak, JL Niu, ZR Li, and Q Zhou.2011. The Effect of Balconies on Ventilation Performance of Low-rise Buildings. Indoor and Built Environment20 (6): 649-660. Boutet, T.S. 1987. Controlling Air Movement: A Manual for Architects and Builders: McGraw-Hill.

ID: 59882
Title: WOUND HEALING ACTIVITY OF COLEUS AROMATICUS IN EXPERIMENTALLY INDUCED DIABETIC MICE.
Author: Kaleshware Muniandy, Zaiton Hassan and Mohd Hafez Mohd Isa
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 627-638 (2013.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Wound healing, Coleus aromaticus, Excision wound model, Dead space wound model, Granulation tissue, Epithelialization.
Abstract: Background. The ethanolic and aqueous extract of C. aromaticus leaves and roots (100 mg kg-1 day-1 for 10 days) were evaluated for its wound healing activity in Monosodium Glumate induced diabetic mice using excision and dead space wound models. Excision wound model was performed on five different groups of mice to measure the progress of wound area for ten consecutive days. Dead space wound model was performed on four different groups of mice to estimate wet and dry granulation tissue as well as to examine the histological appearances of granulation tissue after ten days. Ethanolic extract treated wounds was found to show 76.6 % of wound area reduction when it is compared with controls that exhibited 55.9 % of wound area reduction. The ethanolic extract treated wound had epithelised faster compared to controls. The wet and dry granulation tissue weight has increased significantly in extract treated mice when it is compared with the controls. Extract treated mice exhibited good proliferation of collagen tissues with satisfactory angiogenesis. Coleus aromaticus promotes significant wound healing in induced diabetic mice and evaluation of this activity on human organism is highly suggested.
Location: TE 12 New Biology Building
Literature cited 1: Adam, J., Singer Richard and Clerk, A.F.1999. Cutaneous wound healing. The New England Journal of Medicine. 1(1): 738-746. Ahmad Oryan, Aboutorab T. Naeini and Behrooz Nikhaval, 2010. Effect of aqueous extract of Aloe vera on experimental cutaneous wound healing in rats. Veterinarski Arhiv. 80 (4): 509-522.
Literature cited 2: Ailsa Sharp and Jane Clark, 2011. Diabetes and its effects on wound healing. Nursing Standrd. 25 (45): 41-47. Anand K. Jain, Ashish Dixit and Swaroop C. Mehta, 2012. Wound healing activity of aqueous extract of leaves and roots of Coleus aromaticus in rats. Acta Poloniae Pharmacutica-Drug Research. 69 (6): 1119-1123.

ID: 59881
Title: PRODUCTION, PARTIAL PURIFICATION ND BIOCHEMICAL CHARACTERIZATION OF POLYPHENOL OXIDASE FROM TRICOTHECIUM SP (ENDOPHYTIC FUNGUS OF COCOA PODHUSK)
Author: Sartini, ABD.Rauf Patong, Tjodi Harlim and Pirman
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2013
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 15 (4) 621-626 (2013)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Polyphenol oxidase, Tricothecium sp, Endophytic fungus, Theobroma cacao.
Abstract: Fungal strains are considered to be excellent sources for industrial polyphenol oxidase production. Polyphenol oxidase could be produced by endophytic fungus Trichothecium sp from podhusk of Theobroma cacao L. The aim of this research is to partial purify and characterize of the enzyme. The enzyme was produced by submerged fermentation using media with composition, i.e. 1% yeast extract, 25 ppm Cuso4.5H2O, 0.34 % gallic acid, 0.1% KH2PO4, 0.05 % MgSO4.7H2O. The steps of purification were done by 60-90 % (NH4)2SO4 precipitation, dialyzed, then partial purified by gel filtration chromatography using sephadex G-100, respectively. Polyacrylmide gel electrophoresis of the polyphenol oxidase showed 2 bands. Biochemical characteristics of the polyphenol oxidase are laccase and tyrosinase category. Opimum temperature and pH are 30 ? C and 7.0, respectively. The enzymes obeys Michealis-Menten kinetic with Km and Vmax values being 0.625 mM and 2083 U/mL, catechol as substrate.
Location: TE 12 New Biology Building
Literature cited 1: Brijwani, K., Rigdon, A. and Vadlani, P.V., 2010. Fungal Laccases: Production, Function and Applications in Food Processing. Enzyme Research. 11 pages. Chikezie, P.C. 2006. Extraction and Activity of Polyphenol Oxidase from Kolanuts (Cola nitida and Cola acuminate ) and Cocoa (Theobroma cacao). J.Agricul. and Food Sci.4
Literature cited 2: Duran, N. and Esposito, E. 2001. Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review. Applied Catalysis B: Environmental 28: 83-99. De Faria, R, O. de Moure, R.O Amazonas, V.R., Krieger, A.M.R.L and Mitchell, D.A. 2007. The biotechnological potential of mushroom tyrosinase. Food Technology and Biotechnology. 45 (3): 287-294.

ID: 59880
Title: STUDIES ON THE PATHOGENIC POTNTIAL OF RACES IN SPHAEROTHECA FULIGINEA INFECTING CUCURBITS IN EASTERN UTTAR PRADESH, (INDIA).
Author: Sanjay Gangwar and Kiran Mishra.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 465-468 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Powdery mildew, Races, Cucurbits, Host differential.
Abstract: Pathogenic potential of races in Sphaerotheca fuliginea on cucurbits were analysed in Eastern part of Uttar Pradesh (India). The study showed the occurrence of three races in S. fuliginea in this area. Race-3 was most wide spread and infected most of the cultivated cucurbits. Race-2 was restricted to green melon, Citrullus vulgaris var. fistulosus and Race-1 to sponge gourd, Luffa cylindrical. In addition Race-1 infected a few other cucurbits. L. cylendrica, C. vulgaris var. fistulous and C. sativus are proposed as differential hosts for identification of the three races in S. fuliginea isolates from cucurbits.
Location: TE 12 New Biology Building
Literature cited 1: Ballantyne, B.J. 1975. Powdery mildew of cucurbitaceae: Identity, distribution, host range and sources of resistance. Proc. Linn. Soc. New South Wales. 99: 100-120. Cohen, R., Burger, Y. and Katzir, N. 2004. Monitoring o physiological races of Podosphaera xanthii (Syn.Sphaerotheca fuliginea), the causal agent of powdery mildew in cucurbits, factors affecting race identification and the importance for research and commerce. Phyto-Parasitica. 32: 174-183.
Literature cited 2: Hosoya, K., Narisawa, K., Pitrat, N. and Ezura, H.1999. Race identification in powdey mildew (Sphaerothera fuliginea on melon (Cucurmis melo L.) in Japan. Plant Breeding. 188: 259-262. Kaur, J. and Jhooty, J.S. 1986. Presence of Race-3 of Sphaerotheca fuliginea on muskmelon in Panjab. Indian Phytopath. 39: 297-299.

ID: 59879
Title: PHYSICAL, CHEMICAL AND MICROBIOLOGICAL CHARACTERISTICS OF A DUMP SITE NEAR THE UNIVERSITY OF PORT HARCOURT, PORT HARCOURT IN NIGER DELTA AREA OF NIGERIA.
Author: H.O. Stanley, I.F. Onianwah and P.O. Okerentugba.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 459-464 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Physicochemical, Microbiological characteristics, Dump Site, University of Port Harcourt, Niger Delta, Nigeria.
Abstract: The study was carried on the dump site at the University of Port Harcourt main gate and the characterization was done on microbial and physicochemical properties of the dump. The result of the analysis revealed that the site has pH range of 8.0 to 9.5 and a temperature range of 36 ? C to 42 ? C with a mean annual rainfall of 677.12 mm. The conductivity of the refuse samples (5.5 to 190.5 uscm1) was high due to the presence of ions. The concentration of Cadmium and Lead (1.74 to 2.91 mg/kg and 3.91 to 5.82 mg/kg respectively) were high compared to (0.36 to 0.89 mg/kg) Mercury and this may be attributed to the volatile nature of Mercury. The high values of Biochemical Oxygen Demand and Chemical Oxygen Demand is responsible for the high level of aerobic activities at the site. Further chemical analysis revealed the presence of Hydrocarbon (2.87 to 4.26 mg/kg). The total heterotrophic bacteria count ranged from 2.9 x107 to 9.2 x 107 cfu/ml and 3.0x 109 cfu/g; while total fungal count is 2.7 x 103 to 5.6 x 106 cfu/ml and 1.4 x 105 to 2.6 x 105 cfu/g. Aerobic microorganisms such as Shigella, Samonella, Pseudomonas, Bacillus, Micrococcus, Staphylococcus, Escherichia, Torulopsis, Candida, Saccharomyces, Rhizopus, Aspergillus, Fusarium and Mucor were isolated and characterized from the samples. The presence of heavy metals and pathogenic microorganisms poses danger to public health and as such, requires checks.
Location: TE 12 New Biology Building
Literature cited 1: Allen, A.R. 2001. Containment Landfill: The Myth of Sustainability. J. Eng. Geology. 603-19. Alloway, B.J. 1990. Cadmium. In B.J. Alloway (Ed). Heavy metals in soil. John Wiley and sons.New York. 100- 124.
Literature cited 2: Attahi, F. 1999. Abidjan, Cote d Ivoire. In Managing the Monster. Urban waste and governance in Africa, (ed. A.G. Onibokun) 11-48, IDRC, Ottawa. Aust, A.S., Lamar, R. and Arisoy, M. 1998. Degrading Environmentally persistent organic pollutant compounds. Environmental Contamination and Toxicology. 60: 872-876.

ID: 59878
Title: GROWTH OF Bacillus Megaterium CSK2, Bacillus Subtilis CSK3 AND Bacillus Subtilis CSK4 ISOLATED FROM COAL MIXED SOIL IN DIBENZOTHIOPHENE-CONTAINING MEDIUM.
Author: Megga Ratnasari Pikoli, Pingkan Aditiawati, Akhmaloka, Dea Indriani Astuti and Reni Wijayanti.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 453-460 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Bacillus megateium, Bacillus subtilis, Dibenzothiophene, Desulfurization, Coal.
Abstract: Biodesulfurization is an environmentally friendly technology to reduce organic sulfur in coal by utilizing bacterial metabolism. Isolation and identification of bacteria from coal-mixed soil from South Sumatra has been conducted in order to obtain bacteria which desulfurize organic sulfur in local coal. Three viable isolates, i.e CSK2, CSK3, and CSK4, were obtained by using a mineral medium containing glucose as carbon source and dibenzothiophene as sole sulfur source. Molecular identification of 16 SrRNA gene sequences showed that the isolates had 99% similarity to genus Bacillus, which CSK2 was related to B. megaterium, while the isolates CSK3 and CSK4 were related to B. subtilis. Growth examination of the isolates within 48 hours in the dibenzothiophene in the period of incubation was 37.4, 16.7, and 25.9 percent of the initial 0.1 mM dibenzothiophene, by B. megaterium CSK2, B. subtilis CSK3, and B. subtilis CSK4 respectively. The results showed that the isolates derived from coal-mixed soil have potential to be developed in desulfurization of organic sulfur in coal.
Location: TE 12 New Biology Building
Literature cited 1: British Petroleum. 2013. BP Statistical Review of World Energy June 2013. http: // www.bp.com/content/dam/bp/pdf/statistical -review/statistical_review_of _world_energy_2013.pdf. Constanti, M., Giralt, J., and Bordons, A. 1994. Desulphurization of dibenzothiophene by bacteria. World Journal of Microbiology and Biotechnology. 10 (5): 510-516.
Literature cited 2: Etemadifar, Z., Emtiazi, G., and Christofi, N. 2008. Enhanced desulfurization activity in protoplast transformed Rhodococcuserythropolis.Am.Eur.J.Agric.Environ.Sci 3: 795-801. Gunam, I.B.W . Yaku, Y., Hirano, M., Yamamura, K., Tomita, F., Sone, T. and Asano, K. 2006. Biodesulfurization of alkylated forms of dibenzothiophene and benzothiophene by Sphingomonassubarctica T7b. Journal of Bioscience and Bioengineering. 101 (4): 322-327.

ID: 59877
Title: EARTHWORM MEDIATED BIOREMEDIATION OF PHENOL
Author: Neethu Kamarudheen, Christy George, Ishani Bose, Mythili Sathiavelu and A.Sathiavelu.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 447-451 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Earthworm, Vermicomposting, Phenol, Bioremediation, 4-amino antipyrine.
Abstract: Our study dealt with the phenol bioremediation potential of Earthworms which were collected from a vermicomposting unit in Vellore, Tamil Nadu and maintained under proper growth conditions. Different concentrations of phenol ranging from 10 ppm to 50 ppm wee taken for the assays. All the bioremediation assays were done in the soil with phenol dispersed in water and remediation rates were observed for 24 hours, 48 hours, 72 hours, 96 hours and 120 hours. The initial concentration of phenol was increased eventually and the rate of bioremediation was estimated using 4-amino anti pyrine method. The phenol degradation activity of the gut microflora of the earthworm along with the soil microflora was also observed on a formulated media. The amount of phenol bioremediated was found higher in case of earthworm and not the microflora solely. It was found to be 30 ppm in 48 hours when the initial concentration was 50 ppm.
Location: TE 12 New Biology Building
Literature cited 1: Arun, K., Ashok, M. and Rajesh, S. 2011. Crude oil PAH constitution, degradation pathway and associated bioremediation microflora, International Journal of Environmental Sciences. 1 (7): 1420-1439. Chakrabarty, A.M. and Daubaras, D. 1992. The environment, microbes and bioremediation: microbial activities modulated by the environment. Biodegradation. 3: 125-135.
Literature cited 2: Dipty Singh and Fulekar, M.H. 2007. Bioremediation of phenol using microbial consortium in bioreactor, Innovative Romanian Food Biotechnology. 1: 31-36 Eijsackers, H., Van Gestel, C.A.M., De Jonge S., Muijs B., and Slijkerman D. 2001. Polycyclic Aromatic Hydrocarbon-Polluted Degraded Peat Sediments and Earthworms: A Mutual Interference. Ecotoxicology 10: 35-50.

ID: 59876
Title: MOLECULAR DOCKING STUDIES ON TRANSPOSASE PROTEIN OF STREPTOCOCCUS PNEUMONIAE-TCH8431/19A STRAIN.
Author: Balasankar Karavadi and M. Xavier Suresh
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 443-446 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Pneumonia, Streptococcus pneumonia, TCH8431/19A, Homology, Docking.
Abstract: Streptococcus pneumonia causes mucosal diseases in human upper respiratory tract. TCH8431/19A strain of Streptococcus pneumonia is extremely virulent in pneumococcal pathogenesis which contains Protein coding gene (HMPREF0837_11199) which synthesizes transposase protein. In this article we performed the homology modeling studies of the virulent protein which is synthesized by the protein coding gene and validated he nature of the receptor as a future drug target for TCH8431/19 A Strain of streptococcus pneumonia. We have also identified specific ligands for the above mentioned protein by using the virtual structure based ligand screening approach. Protein-ligand complexes have been analyzed by docking studies using Discovery Studio and interactions have also been visualized along with the validation of pharmacokinetic descriptors.
Location: TE 12 New Biology Building
Literature cited 1: Avery, O.T., MacLeod, C.M., McCarty, M. 1944. Studies on the chemical nature of the substance inducing transformation of pneumococcal types. J. Exp. Med.79: 137-158 Chou, J., Lemaux, P.G., Casadaban, M., Cohen, S.N. 1979. Transposition protein of Tn3: identification and characterization of an essential repressor controlled gene product. Nature. 282: 801-806
Literature cited 2: Joe Dundas et al. 2006. CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues. Nucl.Acids Res. 34: W116-W118. Kleckner, N. 1990. Regulation of transposition in bacteria. Annu. Rev. Cell Biol. 6: 297-327.

ID: 59875
Title: A STUDY ON THE LIME-CEMENT STABILIZED MARINE CLAY.
Author: D.Venkateswarlu, M. Anjan Kumar, G.V.R. Prasad Raju and D.S.V. Prasad.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 437-442 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Cement, Marine clay, Lime.
Abstract: Comprising over 70 % of earth ' s surface, water and its adjacent areas is undoubtedly the most precious natural resources that exist on our planet. Areas surrounded by the coast have been highly utilized since ancient times. Several National and International authorities have mandated to develop coastal structures as part of infrastructure development. The various potential problems associated with these Marine soils include land slippage, slope instability, poor foundation support and poor drainage. Problems often arise when roads or other structures are to be constructed on soft ground. In the case of Flexible Pavements, unless the subgrade is appropriately treated at the construction stage, the transportation cost will increase substantially due to deteriorated pavement performance. There are many methods of stabilization of subgrade marine clay. Treating the soil with lime, cement and lime-cement combination is a unique technique to improve the engineering properties of the marine clay. The main objective of this work is to study the effectiveness of additives at different percentages on the engineering properties of marine clay.
Location: TE 12 New Biology Building
Literature cited 1: Bansal, R.K., Pandey, P.K. and Singh, S.K. 1996. Improvement of typical clay for roads subgrades with hydrated lime. Proceedings of National conference on Problematic Subsoil Conditions, Terzghi-96, Kakinada, India, 1996, pp. 193-197. Basack, S. and Purkayastha, R.D.R.D. 2009. Engineering properties of marine clays from the eastern coast of India. Journal of Engineering and Technology Research. 1 (6): 109.114.
Literature cited 2: Kamon, M. and Nontananandh, S. 1991. Combining industrial wastes with lime for soil stabilization. Journal of Geotechnical Engineering.17 (1): 1-17. Katsutada Onitsuka, Chirdchamin Modmoltin and Masakazu KOUNO 2001. Investigation on Microstructure and Strength of Lime and Cement Stabilized Ariake Clay. Reports of the faculty of science and engineering saga University 30 (1).

ID: 59874
Title: MARKER ANALYSIS AND FIELD SCREENING STUDIES IN RICE FOR BLAST RESISTANCE.
Author: A.Chandrasekar, N.Kumaradivel and R. Rabindran.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 433-436 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Rice, blast, Disease, Marker.
Abstract: Rice improvement for durable resistance to blast is difficult due to its complexity. Rice blast, caused by fungus Magnaporthegrisea, is a serious disease causing considerable economic damage worldwide. Blast is one of the destructive diseases, which causes maximum yield losses in rice. Best way to overcome the disease is to breed for disease resistant cultivars/parental lines of hybrids. The present study was conducted to analyse blast resistance in rice. Rice genotypes viz., VRPI, VRP2, B95-1 X Tetep, BPT5204 X Tetep, CO39 and CO43 were screened for blast resistance in artificial condition. The study indicated that the genes Pi9 and Pi54 recorded resistance reaction to blast pathogen occurring in Tamil Nadu. The blast resistance gene, Pi9 is present in VRP1 and VRP2 and another blast resistant gene Pi54-MAS were already identified for the genes Pi9 and Pi54 respectively. The present study indicated that the tightly linked markers showed polymorphism between resistance and susceptible rice genotypes indicating the possibility of initiating MAS for blast resistance in rice.
Location: TE 12 New Biology Building
Literature cited 1: Ashkani, S., Raffi, M.Y., Rusli, I., M. Sariah, Abdullah, S.N. A., Rahim, H.A. and Latif, M.A. 2012. SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryzasativa L) Plant Mol. Biol. Rep. 30: 79-86. Bonman, J.M. Khush, G.S. and Nelson, R.J. 1992. Breeding rice for resistance to pests. Annu Rev Phytopathol 30: 507-528.
Literature cited 2: Chen, D.H. Zeigler, R.S., Ahn, S. and Nelson, W. 1996. Phenotypic characterization of the rice blast resistance gene Pi2 (t). Plant Dis 80: 52-56. Filippi M.C and Prabhu, A.S. 2001. Phenotypic virulence analysis of Pyriculariagrisea isolates from Brazilian upland rice cultivars. Pesquisa Agropecuaria Brasileira 36: 27-35.

ID: 59873
Title: ARTIFICIAL SCREENING FOR BACTERIAL BLIGHT RESISTANCE AND VALIDATION OF MARKERS FOR MARKER-ASSISTED SELECTION.
Author: A.Chandrasekar, N.Kumaravadivel and R.Rabindran.
Editor: Dr. P.K. Wong, Dr. R.K. Trivedy
Year: 2014
Publisher: Global science publications
Source: Centre for Ecological Sciences
Reference: Asian Journal of Microbiology, Biotechnology & Environmental sciences vol. 16 (2) 429-432 (2014.)
Subject: Asian Journal of Microbiology, Biotechnology & Environmental sciences
Keywords: Rice, Bacterial leaf blight, Disease, Marker.
Abstract: Rice (Oryzasativa L.) is the most important food crop of the world. Breeding for resistance against Bacterial blight (BB), a destructive disease in rice, is a major challenge because of the rich diversity of the pathogen, Xanthomonasoryzaepv.oryzae. Rice genotypes viz., B95-1 X Abhaya (xa13), B95-1 XTetep (Xa21), B95-1 XKavya (Xa 21), B95-1 X Aganni (Xa21), ADT38 and CO43 were screened for bacterial blight resistance in artificial condition. The study indicated that the genes xa13and Xa21 recorded resistance reaction to bacterial blight pathogen occurring in Tamil Nadu. Tightly linked markers xa13-prom and Pta248 are available for the genes xa13 and Xa21 respectively.
Location: TE 12 New Biology Building
Literature cited 1: IRRI-Standard evaluation system.1996. International Rice Research Institute, Manila, Philippines. Jung-Pil, S. Jeung, J.U. Hwan Noh, T.Cho, Y.C. Park, S.H. Park, H.S.Shin, M.S. Kim, C.K. and Jena, K.K. 2013.
Literature cited 2: Kauffman, H.E., Reddy, A.P.K., Hsien, S.P.Y. and Merca, S.D. 1973. An improved technique for evaluating resistance of rice varieties to Xanthomonasoryzae. Plant Dis Rep 57: 537-541. Khush, G.S. and Virmani, S.S. 1985. Breeding rice for disease resistance. In Russell, G.E. (ed), Progress in Plant Breeding, Vol.I, Butterworths, London, 239-279.