Abstract: A new species of Glyptothorax is described form Karim Puzha, Nilambur, Western Ghats. The new species is different from all other known Glyptothorax species, characterized by dorsal fin serrated posteriorly plaited paired fins, adhesive apparatus with distinct central pit and occipital pricess not reaching the basal bone of the dorsal fin. During the course of survey of fishes in the Nilgiri Biosphere reserve (NBR), a specimen of a new Glyptothorax,Sisoridae was collected from the Karim Puzha and its tributary Panna Puzha, Maancheri, Nilmburforest (Kerala), a apart of the Nilgiri Biosphere reserve (NBR) of Western Ghats. Five specimens of Glyptothorax have been collected from river Karim Puzha during the survey. Occurrence of Tor khudree, a rare and threatened fish species of this rivers remarkable. Karim Puzha is a good breeding habitat for Tor khudree.