Abstract: Biogeographically, the hill chain of the Western Ghats constitutes the Malabar province of the oriental realm, running parallel to the Westcoast of India from 8°N to 21°N latitude around 1600 km. Rising up from a relatively narrow strip coast as its western border, the hills reach up to a height of 2800m before they merge to east with in the Deccan Plateau at an altitude of 500-600m. The average width of the mountain range is about 100 km. This bio-region is highly species rich and under constant threat due to human pressure and is considered one of the 18 biodiversity hot spots of the world. The methodology developed to assess biodiversity over the Western Ghats using Remote Sensing involves a synthesis of information derived at several spatial scales.